The purpose of this study is to select various insect species for healing resources and develop a healing program in order to use insect as a healing agriculture. In this study, there are two kinds of breeding kit were developed, one for Gryllus bimaculatus and the other for Oxya chinensis sinuosa. Using these insect breeding kits, we conducted a survey of 60 children and the elderly. In the case of children, the results of the insect breeding satisfaction showed that 30.6% said that the sound of crickets was very good, and 11.1% said that it was good. In addition, the higher the child's awareness of insects, the higher the proportion of children who wanted to raise insects in the future. As a result of a survey of seniors, 45.2% do not like insects and 51.6% are not interested, meaning that most seniors are not very interested in insects. However, the emotions after breeding insects showed positive results, with 45.2% saying their personality became brighter, 48.4% reducing their anger, 48.4% relieving their irritation, 54.8% relieving loneliness, 58.1% feeling more responsible, and 51.6% developing intimacy.

계란의 비린내 강도는 가금류의 종류와 개인의 인식에 따라 다르지만 여러 요인에 의해 발생할 수 있다. 특히 트 리메틸아민(TMA)이 원인으로 밝혀졌다. 주목할만한 점은 리보플라빈이 TMA를 무취의 트리메틸아민-N-옥사이드로 전환시키는 역할을 하는 효소인 플라빈 함유 모노옥시게 나제 3의 활성을 증가시킬 수 있다는 점이다. 본 연구는 난황의 TMA 함량을 분석하여 비린내에 대한 기여도를 평 가하고, 비린내를 방지하는 방법을 개발하는 것을 목적으 로 하였다. 고체상 미세추출-기체 크로마토그래피/질량 분 석법을 사용하여 리보플라빈이 강화, 보충된 사료를 먹인 암탉의 난황에 있는 휘발성 화합물을 감지하고 정량화했다. 또한 샘플 간 휘발성 물질의 상대적 함량을 비교하기 위해 전자코를 이용하여 상관관계 연구를 수행하였다. TMA는 콜린이 함유된사료를 섭취한 가금류의 난황에서 고농도로 검출되었지만 리보플라빈이 보충된 사료를 섭취한 가금류 에서는 검출되지 않았다. 전반적으로, 이 연구는 리보플라 빈이 TMA를 포함하여 계란에 존재하는 휘발성 물질의 양 과 품질에 영향을 미친다는 것을 시사한다. 이러한 발견 이 계란의 비린내를 줄이는 것은 물론 품질 향상에 기여 하기를 기대한다.

본 연구에서는 국내산 돌미나리에서 GABA생성능이 있 는 신규 유산균을 분리, 동정한 결과, Enteroccoccus casseliflavus로 확인되었다. 최근까지 Lactobacillus속과 같 은 GABA 생성 유산균에 대한 보고는 많이 되고 있고, 일 부 Enterococcs속 유산균도 보고되고 있으나 E. casseliflavus 종에 대한 보고는 없었다. 따라서 본 연구에서는 E. casseliflavus PL05 균주에 대한 GABA 생성 최적 조건을 찾기 위하여 배지의 유형, 생육 온도, 초기 pH 조건, 배양 시간, MGS 농도 및 탄소원을 포함한 다양한 조건을 테스 트하였다. PL05 균주는 MRS 혹은 TSB 배지보다 BHI 배 지에서 생육이 잘 되었으며, 배지의 초기 pH는 7-9 조건 에서 가장 생육이 왕성하였고, GABA 생성 조건 역시 유 사한 결과로 확인되었다. GABA의 기질에 해당하는 MSG 의 농도별 GABA 생성량을 조사한 결과, 7%에서 가장 높 은 생성량을 나타내었으나 5%에서도 유사한 수준으로 확 인되어 효율적인 측면에서 5%가 적합할 것으로 판단된다. 탄소원에 따른 생육 및 GABA 생성량은 말토오스를 사용 하였을 때 가장 높은 것으로 확인되었고, 이러한 최적의 조건들로 최종 테스트를 진행한 결과, 24시간째 140.06±0.71 mM의 GABA가 생성되었고, 전환율은 78.95%로 확인되 었다. 또한 반코마이신을 포함한 10개의 항생제에 대한 감 수성을 조사한 결과 내성이 없는 것을 확인하였다.

As the number of North Korean refugees increases in South Korean, their acculturation to life in their host country is coming to be an important social issue. This study explores some clothing-related barriers experienced by North Korean refugees and their moderating effects on acculturation to South Korea. Data were collected using a self-administered survey of 163 female and 37 male North Korean refugees in South Korea aged 20 to 69 years. Descriptive analyses, t-tests, ANOVA, Duncan tests, and moderated multiple regression were conducted using SPSS 20.0 and Process Macro v.3.3. The results show that the North Korean refugees who participated in the study had experienced clothing-related barriers regarding fashion terminology and shopping rituals in South Korea. In particular, those in their 60s perceived more clothing-related barriers than those in their 20s and 30s. Next, the clothing-related barriers experienced by North Korean refugees have a negative moderating influence on the relationship between self-esteem and acculturation in South Korea. This study provides a deeper understanding of the relationship between fashion and the acculturation of North Korean refugees to South Korea. The results of the study can be also helpful of government policy makers, practitioners, and academics to develop education programs for North Korean refugees.

Biofilms of oral microbes can cause various diseases in the oral cavity, such as dental caries, periodontitis and mucosal disease. Electrolyzed water generated by an electric current passed via water using a metal electrode has an antimicrobial effect on pathogenic bacteria which cause food poisoning. This study investigated the antimicrobial activity of electrolyzed waters using various metal electrodes on the floatage and biofilms of oral microbes. The electrolyzed water was generated by passing electric current using copper, silver and platinum electrodes. The electrolyzed water has a neutral pH. Streptococcus mutans, Porphyromonas gingivalis and Tannerella forsythia were cultured, and were used to form a biofilm using specific media. The floatage and biofilm of the microbes were then treated with the electrolyzed water. The electrolyzed water using platinum electrode (EWP) exhibited strong antimicrobial activity against the floatage and biofilm of the oral microbes. However, the electrolyzed water using copper and silver electrodes had no effect. The EWP disrupted the biofilm of oral microbes, except the S. mutans biofilm. Comparing the different electrolyzed waters that we created the platinum electrode generated water may be an ideal candidate for prevention of dental caries and periodontitis.

Explicit environmental attitude measures tend to produce strong socially desirable responses (Bruni & Schultz, 2010; Milfont & Duckitt, 2010; Stern & Dietz, 1994) and fail to capture more than what participants consciously recall (e.g., explicit belief, past behavior, Dunlap, Van Liere, Mertig, Jones, 2000; Schultz, Shriver, Tabanico, & Khazian, 2004). A number of studies have highlighted the popularity of environmental issues in common discourse in various countries, which leads individuals to eagerly express pro-environmental explicit attitudes (Dunlap, 1998; Leiserowitz, 2005; Meijers & Stapel, 2011). Therefore, an explicit measure of environmental attitudes would hardly be an unbiased indicator of prior knowledge and experience when testing the effect of subtle feeling on judgments: although a participant explicitly reports favorable attitudes toward environment or related issues, it does not necessarily mean that she or he has knowledge and experience to hinder the regulatory fit effects. Using the Implicit Association Test, we examined whether the extent to which individuals are implicitly biased toward (or against) sustainability moderates the regulatory focus by construal level interaction. Scores from the indirect measure of attitudes served as the indicator of the strength of associations between the concept of sustainability and evaluations that may result form its frequent pairing with desirable consumption patterns or repeated exposure to persuasive messages promoting sustainability. Specifically, we showed that the regulatory focus – construal level fit effect will be greater among individuals who have not formed significant associations about sustainability since these individuals are more likely to rely on feeling right from regulatory fit rather than using prior experience or knowledge.

캄보디아의 카사바는 최근 벼 다음으로 매우 중요한 작물로 부상하고 있다. 국내 소비와 수출이 증대됨에 따라 지난 10년 동안 급성장을 하고 있다. 최근의 재배면적 변화를 보면 2000 년 16,279 ha에서 2012년 361,854 ha로 급격하게 증가되었다. 생산량도 2000년 147,763톤에서 2012년 7,613,698톤으로 약 52배 늘었다. 단위면적당 수확량을 보면 ha당 2000년 10톤 내 외에서 최근 우기재배의 경우 21톤, 건기의 경우 약 29톤에 이르고 있다. Battambang, Kampong Cham 및 Banteay Mean Chey 등이 주산지이며, 이들 지역의 생산량은 전체의 48%를 차지하고 있다. 수량 감소 주요인은 토양 양분결핍, 재배기간의 단축, 잡초 피해 등으로 분석되었다. 따라서 생산성이 저조한 지역에서도 수량 감소 요인을 개선하게 되면 금후 수량성을 높일 수 있을 것으로 기대하고 있다. 세계 시장에서 캄보디아의 카사바 경쟁력을 높이기 위해서 는 수량성 증대와 생산비 절감이 절실하다. 영양적 측면에서 전분함량 증대, 전분의 기능성 확대, 영양가치의 향상 등도 요 구된다. 가공면에서는 가공공장의 확대, 가공효율 개선, 새로 운 가공기술과 상품 개발 등도 개선되어야 한다. 특히 새로운 시장 개척과 생산, 가공 및 유통 등의 긴밀한 협력체계 구축 이 필요하다 하겠다. 이 밖에도 높은 인플레이션에 대한 인건 비, 농산물 및 서비스가격의 상승 등도 해결되어야 한다. 또한 다수확 품종의 지속적인 공급과 농가의 기술 평준화가 선행되 어야 한다. 지역 및 국제시장에서의 카사바 가격에 대한 정보 도 충분하지 않다. 그리고 농가의 관개시설 미비에 따른 천연 강수량 의존성과 설비 보완업체 부족 등도 문제다. 이러한 점 을 해결하기 위해서는 카사바에 대한 명확한 정책 및 관련기 관의 설립 기반이 마련되어야 하고 기관과 재정의 강화뿐만 아니라 효율적인 마케팅 체계를 다양화하고 카사바 부가가치 의 향상이 필요하다고 본다.

담관내 유두 종양은 미세한 섬유혈관 중심을 감싸는 수많은 엽상의 유두상 주름이 발생하는 것을 특징으로, 종양자체나 혹은 종양의 잔해로 인한 폐쇄 혹은 과도하게 분비되는 점액으로 인해 담관이 막혀, 간내 담관이 확장되는 다발성의 종양으로, 췌관내 점액 유두 종양과 개념적으로 대척되어 이들 사이의 연관성을 연구 중이다. 최근 발열을 주소로 내원한 환자에서 복부초음파에서 간농양이 의심되어, 간농양의 원인을 찾고자 시행한 추가적 영상 검사에서 담관내 유두 종양이 의심되어 수술을 시행한 결과, 고도의 이형성을 동반한 점액 분비하는 간내 담관내 유두 종양으로 진단되었던 1예를 경험하였기에 문헌고찰과 함께 보고한다.

Background : Ginseng (Panax ginseng C.A. Meyer) is one of the most important medicinal plants in Korea, but its yields are often reduced by a variety of root pathogens. The root rot of ginseng is a destructive soil-borne disease caused by Cylindrocarpon destructans (teleomorph: Ilyonectria radicicola). To monitor contamination with C. destructans in ginseng harvested in 2015 were sampled from 57 different growing fields. The spore number of C. destructans was quantified by use of a specific primers and selective media (radicicol) in soils of ginseng fields. Methods and Results : The ginseng samples were surface-sterilized and placed on potato dextrose agar plates for 7 day incubation at 20℃. Emerging fungal colonies were counted primarily based on colony and conidia morphology. Further species level identification was confirmed by ITS rDNA sequencing. For quantification of the soil-borne C. destructans, the genomic DNA was extracted from the soil using a NucleoSpin soil kit (MN, Germany). Density of C. destructans was determined by species specific real time PCR (qPCR). The qPCR was completed by running a melting curve analysis. Conclusion : The C. destructans associated with root rot disease of ginseng were detected in more than 60% in pyeongtaek-1, pochenon-1, jecheon-1, chungju-1 and jinan-4. As results of the study, the correlation between pathogen density and identification clearly clarified in the soil.

The aim of this study was to isolate chicken feather-degrading bacteria with high keratinolytic activity and to investigate cultural conditions affecting keratinolytic enzyme production by a selected isolate. A chicken feather-degrading bacterial strain CH3 was isolated from poultry wastes. Isolate CH3 degraded whole chicken feather completely within 3 days. On the basis of phenotypical and 16S rDNA studies, isolate CH3 was identified as Bacillus thuringiensis CH3. This strain is the first B. thuringiensis described as a feather degrader. The bacterium grew with an optimum at pH 8.0 and 37℃, where maximum keratinolytic activity was also observed. The composition of optimal medium for keratinolytic enzyme production was feather 0.1%, sucrose 0.7%, casein 0.3%, K2HPO4 0.03%, KH2PO4 0.04%, MgCl2 0.01% and NaCl 0.05%, respectively. The keratinolytic enzyme had a pH and temperature optima 9.0 and 45℃, respectively. The keratinolytic activity was inhibited ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, and metal ions like Hg2+, Cu2+ and Zn2+. The enzyme activated by Fe2+, dithiothreitol and 2-mercaptoethanol.

The removal of nitrogen compounds from a wastewater is essential and it is often accomplished by biological process. An aerobic nitrate-removing bacterium was isolated from a municipal sewage treatment plant and soil. On the basis of its morphological, cultural and physiological characteristics and 16S rRNA sequencing data, this strain was identified as Pseudomonas fluorescens, and named as P. fluorescens K4. The optimal conditions of the initial pH and temperature of media for its growth were 7.0~8.0 and 30℃, respectively. P. fluorescens K4 was able to remove 99.9% of nitrate after 24 h in a culture. The strain could grow with a nitrate concentration up to 800 mg/l and was able to remove 99.9% of nitrate after 104 h of incubation. The optimal electron donor was sodium citrate for a nitrate removal. The strain K4 showed a capability of a complete nitrate removal when the initial C/N ratio was 1.0. An effect of the initial seed concentration was observed for a cell of 10% (v/v) for a nitrate removal. Especially P. fluorescens K4 could completely remove 200 mg/l ammonium for 3 days.