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        검색결과 22

        1.
        2017.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        목적: 대학생 소비자의 안경구매행태와 소비가치가 소비만족도에 영향을 미치는지 연구하기 위하여 인구 통계학적 특성에 따라 안경구매행태에 차이가 있는지 확인하고 소비가치가 대학생 소비자의 소비만족도에 영향을 미치는지 확인한다. 방법: 본 연구대상의 표본은 2017년 6월 1일부터 6월 15일까지 익산 소재 대학교의 대학생들을 대상으로 직접 설문방식으로 조사하였고, 총 320부의 설문지를 배포하여 응답이 누락되어 분석에서 제외한 17부를 제외하고 최종 302부를 분석 자료로 이용하였다. 기술통계분석, 빈도분석, 카이스퀘어 검증, 다중회귀분석을 실시하였다. 결과: 조사대상자의 안경구매행태를 살펴보면, 평균안경교체주기는 ‘1년 이상 2년 미만’이 32.1%로 가장 높게 나타났고, 안경교체이유는 ‘도수 변화’가 39.1%로 가장 높게 나타났으며, 안경선택에 영향을 주는 사람 은 ‘나 자신’이 70.2%로 가장 높았다. 안경구매 시 가장 중요한 선택요인은 ‘디자인’이 가장 중요하다고 응답 한 경우가 52.6%로 가장 높게 나타났다. 인구통계학적 특성에 따른 안경구매행태의 차이를 분석한 결과, 여 성보다 남성이, 소득이 많은 사람이, 안경을 착용한지 얼마 되지 않은 대학생들이 더 안경교체를 쉽게 결정 하였다. 남성의 안경교체이유는 ‘안경파손’, ‘렌즈흠집’, ‘유행 뒤처짐’ 순이었으나 여성은 ‘도수 변화’가 51.9%로 압도적으로 높게 나타났다. 안경착용기간이 ‘10년이상’인 집단은 ‘도수 변화’ 비율이 48.8%로 다른 집단에 비해 높았다. 소득수준이 높은 집단이 소득수준이 낮은 집단보다 안경선택이유로 디자인을 최우선 하는 것으로 나타났고 소득수준이 낮은 집단이 높은 집단보다 안경선택이유로 가격을 최우선하는 것으로 나 타났다. 본 연구에서 조사대상자인 대학생들에게 안경구매는 미래보다는 현재에 가치를 둔 소비로 나타났으 며, 쾌락보다는 실용성에 가치를 둔 소비로 나타났다. 소비가치 중 쾌락적 소비가치 수준이 높을수록 통계적 으로 유의미하게 소비만족도에 정(+)의 영향을 미치는 것으로 나타났다. 결론: 대학생의 안경구매에 대한 소비만족도를 높이기 위해 쾌락적 소비가치를 높일 수 있는 다양한 컨 텐츠 개발과 서비스가 필요할 것으로 사료된다.
        4,500원
        2.
        2013.09 구독 인증기관 무료, 개인회원 유료
        Transgenic chickens have been spotlighted as an highly potent bioreactor for their fecundity, short generation time, and eggs associated with mass production of protein. In this study, we generated transgenic chickens exhibiting oviduct specific expression of human growth hormone fused to human transferrin for oral administration. Gene of the modified growth hormone located at downstream ovalbumin promoter (∼3.6 kb) was introduced to stage X blastodermal cell employing retrovirus vector system. Several transgenic chickens were successfully generated. However, genomic analyses showed unexpected deletion within the transgene. The modification of the transgene seemed to occur during germ cell formation because the deletion was detected only from the sperm DNA of the G0 founder animal. There was no evidence of deletion in the somatic cell DNA samples of the same chicken. Consequently, same pattern of the deletion was confirmed in both somatic and germ cells of the G1 progeny.
        4,000원
        8.
        2017.09 KCI 등재 서비스 종료(열람 제한)
        The purpose of this study is to investigate the differences fatty acids and hormonal parameters in the spawning and non-spawning season between the diploid and induced triploid Far Eastern catfish, Silurus asotus. The measured triploids were produced by cold shock for 50 min at 4℃ in May 2014, the spawning season of diploid was in May, and the nonspawning season was designated in January. Estradiol and testosterone and gonadosomatic index of diploid were higher than those of induced triploid in spawning season (P<0.05), and those of diploid in spawning season were higher than nonspawning season. On the other hand, thyroid stimulating hormone and thyroxine of induced triploid were higher than those of diploid in spawning season (P<0.05). Erythrocyte count of diploid was higher than that of induced triploid in spawning season and non-spawning seasons. Mean corpuscular volume and mean corpuscular hemoglobin of induced triploid were higher than those of diploid in both seasons (P<0.05). Percentages of total saturated fatty acids and n-3 polyunsaturated fatty acids of induced triploid were higher than those of diploid in spawning season, but those of diploid were higher in non-spawning season (P<0.05). Percentages of total mono unsaturated fatty acids and total n-6 polyunsaturated fatty acids of diploid were higher than those of induced triploids in spawning season, while those of induced triploid in non-spawning season were higher (P<0.05). Therefore, induced triploids in the spawning season tend to concentrate on growth and lipid-synthesization, whereas, diploids concentrate on reproduction and gonadal maturation rather than on growth. In non-spawning season, growth and lipid- synthesization were not significantly different between diploid and induced triploid.
        9.
        2013.08 서비스 종료(열람 제한)
        The inflammatory response to infections, such as bacteria and viruses is mediated by multiple host factors. The tumor related-genes are the important cytokines in mammals. However, a number of tumor related-genes are not identified in the rock bream. Here, we have reported the identification and molecular characterization of the tumor related genes. The LPS-induced TNF-α factor 1 and 2 (LITAF1, LITAF2), tumor necrosis factor superfamily member 14 (TNFSF14), tumor necrosis factor receptor superfamily member 14 (TNFRSF14) and translationally controlled tumor protein (TCTP), programmed cell death 10 (PCD10) from rock bream are used for the under investigations. The LITAF1 and LITAF2 consist of 138 and 163 amino acids with a conserved LITAF domain. TNFSF14 and TNFRSF14 comprise 266 and 181 amino acid, respectively. TCTP encompasses of 170 amino acid containing two conserved TCTP signatures. Furthermore PCD10 consists of 210 amino acids. Using quantitative real-time PCR, we have obtained expression analysis results of LITAF1 and 2, TNFSF14, TNFRSF14, TCTP, PCD10 in the various tissue. Compared to the control, the tumor related genes mRNA is detected at a high levels in gill (LITAF1, TCTP), intestine (LITAF2), liver (PCD10), spleen (TNFSF14) and RBC (TNFRSF14). We have also performed gene expression analysis in the kidney, spleen, liver and gill after challenging with Streptococcus iniae, Edwardsiella tarda and Red seabream iridovirus. We have acquired the dynamic regulated mRNA expression to each of pathogen according to the tissue. Expression of tumor related-genes mRNA are significantly increased by infected with pathogens in most of the tissue. But oddly, PCD10 mRNA is expressed significantly decreased by S. iniae infection in all of tissues. Our results reveal that rock bream tumor relatived-genes may be involved in rock bream immune responses to pathogen infections, as well as, they also act like potential biomarkers for innate immunity.
        10.
        2012.09 서비스 종료(열람 제한)
        Translationally controlled tumor protein (TCTP) is one of important immune regulator. TCTP has been implicated in cellular processes including the cell growth, cell cycle progression, apoptosis regulation and the protection of cells against various stress condition. In this study, we cloned and characterized TCTP from rock bream (Oplegnathus fasciatus), which is an economically important species in the Korea aquaculture industry. The Full-length of rock bream TCTP (RbTCTP) cDNA was 1041 bp and contained an open reading frame (ORF) of 513 bp, which encoded 170 amino acid sequence. The 5' untranslated region (UTR) was 90 bp while the 3' UTR was 538 bp, containing a polyadenylation signal (ATTAAA). The identity of amino acid sequence was 76%, 75% and 74% in tilapia, orange-spotted grouper and Japanese seaperch, respectively. The positions of microtuble-binding region, Ca⁺ binding region and TCTP signature regions in RbTCTP were similar with those of other fish species and mammalian. The RbTCTP mRNA was expressed highest in the muscle. Expression of TCTP mRNA were significantly variable according to injection of red seabream iridovirus (RSIV), Streptococcosis (S. iniae) and Edwardsiella tarda (E. tarda).
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