This is analyzed using the finite element method which is appling excellent isoparametric curve element in the aspect of large usages of dynamic responses in which is regarding geometric and material nonlinear of a large scale shell structure of an airplane, a submarine, a ship, and an ocean structure. The solution of dynamic equations is got by direct integration method using time-stepping procedure and regarding Central Difference Method of the both solutions. But because formal matrix factorization is not necessary in each time step and it does not take less time to compute relatively, this method must be regarded very few time steps on the condition. Axisymmatric shell problems are inspected using 8 node Isoparametric element in this paper. Partial axisymmatric spherical shell is used as a model to analyze axisymmatric nonlinear dynamic behavior regarding. Total Lagrangian formulation in geometric nonlinear behavior and elastio-viscoplastic in material nonlinear behavior.

한국산 멧누에(Bomdyx mandarina)chorion 유전자의 형질 발현기구를 규명하기 위한 연구의 일환으로 본 실험을 실시했다. 멧누에의 난각구조를 주사형 전자현마경에 의해 관찰한 결과 매우 특이적인 구조가 인정되었다. 즉 원추상의 불규칙 돌기에 의한 돌기구조층과 이 돌기 구조층을 덮고 있는 한 층의 얇은 덮개 구조층이 그것이다. 2차원 전기영동법에 의해 chorion 단백질을 분석한 결과, 난각을 구성하는 주요 단백질 성분은 등전점 4~6, 분자량 6~30 kd로 밝혀졌다. 특히 특이난각구조와 관련된 특이단백질 성분을 검출하였으며 이들의 대부분은 고cysteine 단백질인 것으로 추정했다. 이상의 연구결과에 의해 멧누에 난각의 특이 구조 형성에 따른 유전자발현기구 규명을 위한 기초자료가 얻어졌다.

본 연구에서는 개선된 degenerated 쉘 유한요소의 탄소성 및 기하학적 비선형 해석에의 적용성을 고찰하였다. 본 연구의 개선된 degenerated 쉘요소는 shear locking 해결에 우수한 결과를 보인 가정된 전단변형도를 대치사용하고, membrance locking 현상을 제거하기위해 평면내 변형도의 구성시 감차적분을 행하며, 쉘요소 자체의 거동을 보완하기위해 비적합변위형을 선택적으로 추가하였다. 본 요소는 shear/membrance locking이 발생하지 않으며, 전달가능한 거짓 영에너지모드도 나타나지않는다. 소성변형 정형화에서는 적층모델을 사용하며, 재료는 von Mises항복조건을 따른다고 가정한다. 유한변형을 고려한 기하학적 비선형 방정식을 total lagrangian수식화를 사용하여 정형화 하였고, 비선형 방정식은 하중제어 및 변위제어법을 사용한 Newton-Raphson 반복법으로 반복 계산한다. 여러 예제해석을 통하여 본 개선된 degenerated 쉘 유한요소의 정확도를 고찰하였다.

p- 收敬有限要素法은 補間~f!{의 ìxf!{ p를 증가시키는 동안 해석하려 는 領域의 分劃올 固定시키는 有限要素해 석 의 새 로 운 접 근 방볍이다 이 논분에서 는 混合寫像함수에 기 초 한 새로 운 p-~~ p皆層要素 뜰 이용한 젤 모 델 의 컴퓨터 i훌行에 초점 올 두었다. Pinch Test 문 제와 원 통 형 헬지붕문제 를 통해 제안 된 웰 모 델 의 剛體運動， round-off 오차， 그 리 고 收飯性둥 이 검토되 었다

본 연구에서는 쉘구조물의 해석을 위한 개선된 degenerated 쉘유한요소를 제시하였다. 본 연구의 개선된 degenerated 쉘요소는 shear locking 해결에 우수한 결과를 보인 가정된 전단변형도를 사용하고, membrane locking 현상을 제거하기 위해 평면내 변형도의 구성시 감차적분을 행하며, 쉘요소자체의 거동을 보완하기위해 비적합 변위형을 선택적으로 추가하였다. 본 요소는 기존 degenerated 요소계열에서 가장 큰 문제점중의 하나인 locking 현상과 전달가능한 거짓영에너지모드가 발생하지 않으며, 조각시험도 통과한다. 본 개선된 쉘요소의 거동을 알아보기위해 다수의 예제시험을 행하였다. 수치시험결과 본 요소는 빠른 수렴성과 안정성을 보여준다.

이 연구에서는 새로운 웰要素分解의 方法을 定立하고， 이에 의거하여 간단하고， 효율성이 높고， 普遍性 이 큰 웰 有限要素룹 개발하고자 하였다. 實際의 要素는 廠念的인 Translational Element 와 Difference Element 로 分解되며， 要素의 영알f立벼힘훌t는 이 두 成分要-素의 變{立며힘~를 결합하여 얻는다. 要素分解의 基#ζ假定블 단리 함에 따라서 세 가지 의 基本形要素에 도달헬 수 있다 基本形要素칠 보완하여 Locking現像을 제가하고 수렴성을 높히는 방안으로서 減훌積分， 內部自有度의 追加및 Mixed Fomulation을 검토하였으며， 要素의 Spurious Mode를 힘j뼈하는 방법을 고안하였다. 혔植 分析을 통해서 要素의 有效性과 했率性을 검정하였다.

1. 반구형 각의 분포 및 집중하중에 의한 좌굴응력 해석은 변형된 각의 형상에 따라 타원체 각의 응력으로 해석함이 타당하다. 2. 일정 한계이상의 형상계수를 갖는 반 구형각에 대하여서는 재료상수를 형상계수의 승수로 고려한 수정된 임계좌굴 하중으로 탄소성 좌굴을 판정함이 더 양호한 결을 준다. 3. 탄소성 좌굴에 있어서 소모된 소성변형 에너지를 계산하기 위하여 항성변형 에너지를 계산하기 위하여 항상선를 따르는 에너지법을 이용하면 양호한 결과를 얻을 수 있다.

한국국적의 유조선 35척의 외판 및 격벽의 부식도를 선령별로 조사한 결과를 요약하면 다음과 같다. 1. 선령 12년급의 외판의 평균부식도는 경흘수선 및 하부 만곡부선에서는 선수부가, 건현 및 상부만곡부선에서는 중앙부가, 양재흘수선에서는 선미부가 가장 높다. 2. 선령 20년급의 외판의 평균부식도는 경흘수선 및 상.하부만곡부선에서는 선수부가, 건현중앙선에서는 중앙부가, 만재 흘수선에서는 선미부가 가장 높다. 3. 측수조와 중앙유조의 횡격벽의 평균부식도는 어느 선령에서나 정부선, 상부선, 중앙선에서는 측수조가 높고, 하부선과 저부선에서는 중앙유조가 높다. 4. 중앙유조와 양측 유조의 횡격벽부식도 어느 선령에서나 중앙유조의 횡격벽 쪽이 높다. 5. 중앙유조의 종.횡격벽의 부식도는 격벽의 중앙선상부에서는 어느 선령에서나 종벽부 쪽이 높다. 6. 내부수조 및 중앙유조의 횡격벽과 같은 높이에 있는 선각외판의 건현부의 평균부식도를 비교하면, 격벽상부의 부식도가 외판의 그것보다 크다.

한국국적을 가진 일반화물선 30척과 원목운반선 15척의 외판부식도를 선령별로 조사한 결과는 다음과 같다. 1. 일반화물선 1) 전평균부식도는 어느 선령급에서나 경흘수선부가 가장 크다. 2) 선수미방향의 전평균부식도는 어느 선령급에서나 선수부가 중앙부나 선미부보다 크나, 큰 차이는 없다. 3) 깊이 방향의 부식도는 선령 16년 이하에서 건현부, 만재흘수선부, 하부만곡부, 경흘수선부의 순으로 크다. 또 35년에 있어 부식도는 경흘수선부와 만재흘수선부는 건현중앙부에 약 3배, 상부만곡부는 그것의 약 2배 되어 수선부가 가장 부식이 심함을 나타낸다. 2. 원목운반선 1) 전평균부식도는 어느 선령급에서나 상부만곡부가 가장 크다. 2) 선수미방향의 부식도는 어느 선령급에서나 선수부가 가장 크나, 큰 차이는 없다. 3) 깊이 방향의 부식도는 어느 선령급에서나 건현부가 가장 작고, 상부만곡부가 가장 커서 그 부식도는 건현부의 약 2배가 된다. 3. 일반화물선과 원목운반선. 원목운반선의 부식도의 전평균은 일반화물선의 그것의 약 3배나 된다.

To improve the environmental management and resources, in this study, we aimed to investigate the effect of adding oyster shell powder to Hanwoo manure on its characteristics and microbial composition during the storage period. Additives were deposited on top of the manure surface at the rate of 0, 0.5, and 1% of oyster shell powder per 200 g of Hanwoo manure in a plastic container with three replicates; however, untreated manure litter served as the control. Manure characteristics (dry matter, organic matter and crude ash) and microbial composition (lactic acid bacteria, yeast, Bacillus subtilis, Salmonella, and E.coli) were evaluated at day 0, 2, 4, and 8. Manure characteristics exhibited an effect on dry matter, organic matter, and crude ash at day 2 and 8 (p<0.05), and not for day 0 and 4 (p>0.05). With the exception of yeast content at day 4 of storage, lactic acid bacteria, yeast, Bacillus subtilis, Salmonella, and E.coli exhibited no significant differences in all conditions during the storage period. Conclusively, addition of 1% oyster shell powder to Hanwoo manure resulted in slightly better manure characteristics; however, its microbial composition remained unchanged.

To evaluate the remediation performance of recycled oyster shell powders to control nutrients release from polluted sediments. Different types of recycled oyster shell powder were applied on separated bottom sediments. The first type of oyster shell powder is Calcined Oyster Shell Powder (COSP) and another consist of ultrasonicated oyster shell powder (SOSP) which were composed of calcium peroxide. The recycled oyster shell powders were improving the water quality as slow oxygen releasing compound. The experimental results indicated that the Dissolved Oxygen (DO) in the treated columns were higher than the control column. pH was increased in the both experimental columns due to the hydrolysis of CaO2. Meanwhile, recycled oyster shell powders could prevent the nutrients (nitrogen and phosphorus) release from sediments into the overlying water. In addition, the total nitrogen and total phosphorus concentrations of the COSP applied column were decreased 27% and 20% compared to the control column respectively and the SOSP applied column were decreased 33% and 27% compared to control in the overlying water. It was proved that, COSP and SOSP can effectively adsorb phosphorus from sediments and prevent phosphorus release into overlying water from bottom sediments. In conclusion, COSP and SOSP applications was increased DO in the overlying water and nutrient released controlled effectively from the sediment.

The purpose of this study was to investigate the egg freshness and eggshell characteristics of old laying hens by adding sea urchin shell powder to the feed of such hens to achieve the objectives continuously pursued by environmental management, such as business profitability and waste resource utilization. A total of 90 Hy-Line Brown layers (66 weeks of age) were randomly divided into 2 treatments with 3 replicates, each of which consisted of 15 older layers, for 4 weeks. These treatments included a control (i.e., 0%) and 2% sea urchin shell powder. The 2% supplementation of sea urchin shell powder increased the Haugh unit (HU) at 2 and 4 weeks and eggshell strength at 4 wks compared to the controls (p<0.05). The HU at 0 weeks, eggshell thickness from 0 through 3 weeks, and eggshell strength at 0 and 2 weeks were not different between treatments (p>0.05). These results suggest that the addition of 2% sea urchin powder to the feed of laying hens improves egg freshness and eggshell strength in old laying hens.

자원의 재활용 관점에서 굴패각의 소성에 관한 연구가 많이 진행되고 있다. 굴패각을 소성시켜 만들어진 생석회는 건식으로 사용되기도 하고 물과 반응을 시켜 액상소석회로 변환시킨 뒤 사용되기도 한다. 그러나 굴패각은 석회석과는 약간 다른 소성 및 액상소석회 변화의 특성을 보여준다. 본 연구에서는 굴패각과 이를 비교하기 위한 석회석을 소성시켜 생석회를 만든 후 이를 다양한 온도의 물과 반응시켜 액상소석회로 변환 실험을 실시하였다. 액상소석회로 변환 후 150 μm의 체를 이용하여 거르고 액상소석회로의 전환률을 계 산하였다. 소성된 석회석은 모든 온도에서 액상소석회로 전환되었다. 그러나 굴패각의 경우 본 연구의 실험 조건 중 30oC와 50oC에서 액상소석회로 변환되지 않고 오히려 물과의 반응을 통하여 만들어진 Ca(OH)2의 존 재로 질량이 증가하였으며 90oC에서도 석회석 보다는 낮은 액상소석회 전환률을 보여주었다. 굴패각에서 보여주는 이러한 차이는 굴패각의 각주층과 진주층에서 발견되는 단백질의 일종인 콘키올린이 높은 온도에서도 분해되지 않아 물과의 반응을 감소시켜 생기는 결과로 일부 설명할 수 있다. 그러나 콘키올린이 존재하지 않 는 초크층에서도 석회석 보다 액상소석회의 변화률이 낮음을 보여준다. 이것은 석회석에는 거의 존재하지 않 으나 굴패각에서 미량으로 존재하는 Na에 의하여 소성 시 패각의 방해석이 공융용융체 형성과 같은 추가적인 반응에 의한 것으로 생각된다.

In this study, we performed a sediment elution experiment to evaluate water quality in terms of phosphorus, as influenced by the dissolved oxygen consumed by sediments. Three separate model column treatments, namely, raw, calcined, and sonicated oyster shell powders, were used in this experiment. Essential phosphorus fractions were examined to verify their roles in nutrient release from sediment based on correlation analyses. When treated with calcined or sonicated oyster shell powder, the sediment-water interface became “less anaerobic,” thereby producing conditions conducive to partial oxidation and activities of aerobic bacteria. Sediment Oxygen Demand (SOD) was found to be closely correlated with the growth of algae, which confirmed an intermittent input of organic biomass at the sediment surface. SOD was positively correlated with exchangeable and loosely adsorbed phosphorus and organic phosphorus, owing to the accumulation of unbound algal biomass-derived phosphates in sediment, whereas it was negatively correlated with ferric iron-bound phosphorus or calcium fluorapatite-bound phosphorus, which were present in the form of "insoluble" complexes, thereby facilitating the free migration of sulfate-reducing bacteria or limiting the release from complexes, depending on applied local conditions. PCR-denaturing gradient gel electrophoresis revealed that iron-reducing bacteria were the dominant species in control and non-calcined oyster shell columns, whereas certain sulfur-oxidizing bacteria were identified in the column treated with calcined oyster powder.

Bioremediation in situ is heavily dependent on the oxygenic environment which would privide the dwelling microorganism with sufficient oxygen. The situation could be easily resolved with supply of an Oxygen Releasing Compound (ORC). In this paper we prepared that sort of material out of oyster shell powder (mostly calcium carbonate) that prevails every shore areas of the country. We used two different oxidizing methods in the first step of the whole manufacturing process–conventional heating in a furnace and an ultrasound generator to obtain calcium oxide. Then that calcium oxide was further oxidized into calcium peroxide which may release oxygen under a moisturized condition. The oxygen releasing experiments were run to test the performance of our products, and to determine the gas kinetics during the experiments. Interestingly, calcium peroxide derived from ultrasound treatment was much more energy-effective as ORC than that from furnace heating although the heat derived process was better than that of ultrasound in terms of oxygen content and its releasing rate. We also found that most of the data collected from the gas releasing experiments fairly supported an ordinary 1st order kinetics to oxygen concentration, which shaped a sharp discharge of oxygen at the very early moment of each test.

In this study, the anti-oxidant, whitening, and anti-wrinkle effects of Castanea crenata inner shell extracts processed by enzyme treatment and pressurized extraction were investigated. The Castanea crenata inner shell was first hydrolyzed using celluclast, viscozyme, or hemicellulase. Then, it was subjected to pressure extraction for different durations (30, 60, and 120 min). The yields of the Castanea crenata inner shell extracts processed by different enzyme treatments followed by pressurized extraction for different times are in the range of 12.42-29.80%. The total polyphenol, flavonoid, and tannin contents of the C30m (celluclast enzyme and autoclave extracts at 30 min) extract were 15.48, 10.82, and 15.82 g/100 g, respectively. The total sugar content of the H120m(hemicellulase enzyme and autoclave extracts at 120 min) extract is 61.07 g/100 g. The 1,1-diphenyl-2-pycrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities of the C30m extract at 1,000 μg/mL are 89.20, and 81.96%, respectively. The superoxide radical scavenging and ferric-reducing antioxidant power of the C30m extract at 1,000 μg/mL are 67.63% and 1,324.79 μM, respectively. Further, the tyrosinase and elastase inhibition activity of the C30m extract at 1,000 μg/mL are 61.32, and 61.06%, respectively. Our results indicate that the Castanea crenata inner shell extracts processed by enzyme treatment followed by pressurized extraction could have beneficial effects on facial skin and they should be considered for use in new functional cosmetics.

The purpose of this study was to evaluate the effect of sea urchin shell powder, used in broiler diet, on Esherichia coli and Salmonella in litter produced by the broilers. A total of 120 broiler chickens were fed 1 of 3 treatment diets (10 chickens per pen) in a randomized block design treatments with 4 replications. Sea urchin shell powder was used in the concentrations of 0.5% and 1% in the basal diets; the control diet was constituted of basal diet. During the 3-week feeding trials, none of the treatments significantly affected the E. coli populations in poultry litter at weeks 0 and 1, nor did they affect the and S. enterica populations at weeks 1 and 3. However, dietary sea urchin shell powder addition affected the population of E. coli at weeks 2 and 3, and that of S. entericaat weeks 0 and 2 (P<0.05). It is therefore concluded that the use of dietary sea urchin shell powder (0.5% and 1%) will be beneficial enough to reduce E. coli, rather than S. enterica in poultry litter over short-term periods.

To produce palm kernel shell (PKS) biocrude oil, a bubbling fluidized bed pyrolyzer was used with different sample sizes and reaction temperatures. The PKS sample sizes used were 0.1 ~ 0.4 mm, 0.4 ~ 1.0 mm, and 1.0 ~ 2.0 mm and the reaction temperature were 465oC, 490oC, 530oC, 560oC, and 590oC. The yield of PKS biocrude oil increased with decreasing the sample size. The maximum yield of PKS biocrude oil was 47.31% at 560oC with a PKS sample size of 0.1 ~ 0.4 mm. In addition, the maximum energy yield of PKS biocrude oil was 45.05% at 560oC and size 0.1 ~ 0.4 mm. Among the characteristics of PKS biocrude oil, the high heating values are from 15.98 MJ/Kg to 20.29 MJ/Kg, the moisture content is from 20.14wt.% to 31.57wt.%, and the viscosity ranges from 0.0117 N s/m2 to 0.0408 N s/m2. In addition, proximate analyses and elemental analysis of PKS biocrude oil were conducted.

A series of laboratory tests were carried out to verify the strength characteristics of soil-cement improved by the biomineralization of microorganisms and to evaluate the utilizations of soil-cement mixed with oyster shell as an alternative aggregate. The higher the mixed oyster shell ratio, the lower the specific gravity and dry density. The mixing processes were more likely to change the liquid limit and plasticity index of the soil-cement into those for sand. The uniaxial compressive strength of soil-cement mixed with 20% oyster shell and 1 × 103 cells/mL of microorganisms satisfied the requirements of 3,000 kPa for seven days of curing in the Standard Specification for Public Works.

Background: There is an increasing surplus of chestnut that are abandoned due to their failure to meet customer awareness. Thus, we investigated the anti-proliferative and apoptotic effects of chestnut (Castanea crenata) inner shell extracts in hepatocarcinoma HepG2 cells as a potential source of anti-cancer materials. Methods and Results: Distilled water extract (CI-W) and ethanol extract (CI-E) were prepared from chestnut inner shell and evaluated their anti-proliferative effects in vitro. Each extract significantly decreased the cell viability of HepG2 cells in a dose- and time-dependent manner. Indeed, the morphology of HepG2 cells treated with CI-W or CI-D was distorted to shrunken cell masses. Furthermore, it was revealed that their extracts induced cell death as evidenced by increased reactive oxygen species (ROS), formation of apoptotic body and condensation. In addition, Their extracts clearly modulated the down regulated of Bcl-2 (anti-apoptoic)/ Bax (pro-apoptotic) family and cleaved caspase-3 as an effector caspase in a dose-dependent manner. Conclusion: These results indicate that the extracts of chestnut inner shell can be used as an anti-proliferative therapeutic agent or functional food.