This study was conducted to establish the optimal temperature condition before oocyte activation in B6D2 F1 mouse. In experiment 1, two embryo culture media (CZB vs KSOM) were evaluated for the development of activated mouse oocytes. Parthenogenetic embryos cultured in KSOM showed better blastocyst development than ones cultured in CZB(56.2% vs 81.0%, p<0.01). Two-hour of pre-incubation before activation significantly reduced the number of hatched blastocysts in KSOM (22.0% versus 8.8%, p<0.05). In experiment 2, recovered oocytes were pre-incubated at different temperature conditions before activation. The experimental groups were divided by 5 as follows. Group A: pre-incubation for 120 min at 37℃, Group B: pre-incubation at 37℃ for 90 min then at 25℃ for 30 min, Group C: pre-incubation at 37℃ for 60 min then at 25℃ for 60 min, Group D: pre-incubation at 37℃ for 30 min then at 25℃ for 90 min, and Group E: pre-incubation at 25℃ for 120 min before activation. Group A (67.6%) and B (66.7%) showed better development to the blastocyst stage than other groups (Group C: 50.0%, Group D: 49.2%, Group E: 33.3%, p<0.05). The present study indicates that the temperature before activation affects the development of B6D2 F1 mouse parthenogenetic oocytes and exposure to room temperature should be limited to 30-min when the oocytes are left in HEPES-buffered medium for micromanipulation.

잎응애류의 포식성 천적인 민깨알반날개의 실내사육에 필요한 몇 가지 생태적 특성 즉 산란 습성, 성충우화율, 저장온도 그리고 귤응애의 밀도억제효과를 시험하였다. 민깨알반날개 암컷성충은 유자잎 뒷면에 를 산란하였으며, 그중 를 귤응애의 탈피각이나 배설물로 알 표면을 피복하였다. 과수원 내 밭토양에서는 민깨알반날개의 우화율이 였는데, 밭토양 대신 원예용상토를 사용했을 때 로 향상되었다. 대부분의 유충은 지면으로부터 2 cm이내 깊이에서 번데기가 되었다. 성충 저장에 가장 적합한 온도는 발육영점온도인 이며, 저장 10, 20, 30일 후에 생존율은 각각 96.7, 73.3, 였다. 유자에 발생한 귤응애 방제를 위한 효율적인 방사비율은 150:1 미만으로 방사하였을 때 가장 효율적이었다.

본 연구는 가축유전자원의 효율적 보존을 위해 정조세포를 줄기세포 형태로 장기보관하면서 필요에 따라 증식, 분화를 통해 가축의 복원에 활용하기 위한 연구의 일부로 진행되었다. 정조세포를 분리하여 배양한 결과 배양온도는 다른 세포들과는 달리 에 세포분열이 활발하였으며, TCM199에 FCS를 첨가한 배양액과 세르톨리세포 공배양으로 정조세포의 배양을 지지하였다. 40일령이 지나면서 정조세포 콜로니 즉 germline stem cells를 형성하였으며, 일부에

The main goal of this work is to study the effects of temperature and volume fraction of fiber on the Charpy impact test with GF/PP composites. The critical fracture energy and failure mechanisms of GF/PP composites are investigated in the temperature range of 60℃ to -50℃ by impact test. The critical fracture energy increased as the fiber volume fraction ratio increased. The critical fracture energy shows a maximum at ambient temperature and it tends to decreases as temperature goes up or goes down. Major failure mechanisms can be classified such as fiber matrix debonding, fiber pull-out and/or delamination and matrix deformation.

장미 식물공장식 양액재배는 고품질의 절화를 대량으로 계획 생산할 수 있는 시스템으로 장미 수출증가에 따라 기존의 재배 방식에서 식물공장 형태로의 전환이 필수적이다. 또한 식물공장에서의 경제적이고, 효율적인 생산을 위한 지하부 환경의 최적 관리 기술 개발이 절실한 실정이다. 따라서 장미 식물공장에 적합한 single-stemmed rose 수경재배시 배양액의 온도과 같은 지하부 환경요인이 장미의 생육과 품질에 미치는 영향을 알아보고자 실험하였다. 배양액의 온도에 따른 'Red Velvet'의 광합성률, 증산량, 생육 및 수량이 15~20℃에서 높게 나타나 적정 배양액 온도임을 알 수 있었다. 반면에 'Vital'은 10~15℃에서 생육이 가장 왕성하고 30℃의 고온에서는 거의 생육이 이루어지지 않아 'Red Velvet'과는 달리 고온의 배양액에 대한 적응력이 낮은 것으로 나타났다.

The microstructure and mechanical property of hot-pressed composites with a different temperature for atmosphere changing from H to Ar have been studied. When atmosphere-changed from H to Ar gas at 145, the hot-pressed composite was characterized by inhomogeneous microstructure and low fracture strength. On the contrary, when atmosphere-changed at low temperature of 110 the composite showed more homogeneous microstructure, higher fracture strength and smaller deviation in strength. Based on the thermodynamic consideration and microstructural analysis, it was interpreted that the Cu wetting behavior relating to the formation of CuAlO is probably responsible for strong dependence of microstructure on atmosphere changing temperature. The reason for a strong sensitivity of fracture strength and especially of its deviation to atmosphere changing temperature was explained by the microstructural inhomogeneity and by the role of CuAlO phase on the interfacial bonding strength.

In the present study, the focus is on the analysis of the effect of the mold dimensions on the temperature distribution of a die during plasma activated sintering. The temperature distribution of a cylindrical mold with various dimensions was measured using K-type thermocouples. The temperature homogeneity of the die was studied based on the direction and dimensions of the die. A temperature gradient existed in the radial direction of the die during the plasma activated sintering. Also, the magnitude of the temperature gradient was increased with increasing sintering temperature. In the longitudinal direction, however, there was no temperature gradient. The temperature gradient of the die in the radial direction strongly depended on a ratio of die volume to punch area

Synthesis gas is a high valued compound as a basic chemicals at various chemical processes. Synthesis gas is mainly produced commercially by a steam reforming process. However, the process is highly endothermic so that the process is very energy-consuming process. Thus, this study was carried out to produce synthesis gas by the partial oxidation of methane to decrease the energy cost. The effects of reaction temperature and flow rate of reactants on the methane conversion, product selectivity, product ratio, and carbon deposition were investigated with 13wt% Ni/MgO catalyst in a fluidized bed reactor. With the fluidized bed reactor, CH4 conversion was 91%, and Hz and CO selectivities were both 98% at 850℃ and total flow rate of 100 mL/min. These values were higher than those of fixed bed reactor. From this result, we found that with the use of the fluidized bed reactor it was possible to avoid the disadvantage of fixed bed reactor (explosion) and increase the productivity of synthesis gas.

착색단고추 비닐하우스 토경재배시 품종별 온도조건에 따른 열과 발생원인을 구명코자 하였다 열과발생률은 품종간에는 Fiesta가 Jubilee보다 높았고 온도처리간에는 34℃ 고온구에서 높았다. 그리고 환경조건이 좋아지는 4월 이후에 급격히 증가하였다. 과육특성 조사결과 Fiesta품종과 고온처리구에서 과육경도와 건물률이 낮았으나 과육두께는 경향이 일정치 않았다. 과육의 무기성분 함량은 품종간, 온도처리간에 차이가 없었다. 수확시 뿌리상태는 Fiesta품종이 Jubilee보다 좋았고, 온도처리간에는 차이가 없었다. 이상의 결과로 과육의 경도, 건물률, 치밀도 및 뿌리상태 등이 열과발생에 직접적인 영향을 미치고 과육두께나 과육의 무기성분 함량등은 열과발생과 상관이 없는 것으로 나타났다.