본 연구는 방충망 시설이 생육환경에 미치는 영향을 구명하기 위한 기초자료로 활용되도록 무봉지 재배를 위한 망 시설 테스트베드를 설치하고 온도, 습도, 풍속 및 일사량을 수집한 기상자료를 분석하여 망 시설 내·외부의 미기상 특성을 확인하고자 하였으며, 기상자료를 분석한 결과는 다음과 같다. 온도와 습도의 경우 망의 피복 여부에 따라 0.2oC, 3.5~4.7%의 차이가 발생하였으며 실험구 대비 대조구에서 수분부족분이 증가하는 경향을 보였다. 외부와 망 시설 내부에서 일사 투과율은 격자 크기에 따라 큰 차이를 나타냈다. 특히 격자 크기가 2mm인 경우 외부와의 일사 투과율이 최대 50%정도까 지 감소하는 것으로 나타났으며, 이는 과실 생육에 큰 영향을 미칠 수 있기 때문에 격자 크기 선택에 있어서 고려해야할 요소로 판단된다. 태풍 솔릭 내습시의 망 시설 내?외부의 풍속을 비교한 결과, 7 m/s이상의 고풍속 일 때 격자 크기가 4 mm 망 시설은 40% 이상, 2 mm 의 경우 50% 이상의 풍속 감소율을 보였다. 또한 풍속 저감 효과로 인한 낙과 피해도 현저하게 감소하는 것으로 나타났다. 이러한 연구결과는 망 피복에 따른 과수의 생육환경에 미치는 영향과 최근에 잦은 이상기상 발생으로 망 시설을 적용한 이상기상 피해저감 효과 등의 기초자료로 활용될 수 있을 것으로 판단된다.

생활권 수목은 도시림, 생활림, 가로수 등 국민들의 일상생활을 하는 구역이나 장소에 식재된 수목으로 산림이 아닌 지역의 수목으로, 도시민들이 쉽게 접근⋅이용 할 수 있는 공간이다. 현재 생활권 수목의 해충 관리를 위해 농약을 살포하는 화학적 방제가 주로 이루어지고 있으며, 경기도 지역 아파트의 경우 수목관리를 위해 65%는 1년에 4회 이상 약제를 살포하는 것으로 나타났다. 주요 수목 해충 129종 중 방제 약제가 등록된 해충은 33%인 42종뿐으로, 생활권 수목의 화학적 방제는 약제의 오남용 등의 문제가 발생하고 있다. 생활권 수목의 해충 관리를 위해서는 생물적 방제방법과 같은 친환경적 방제방법에 대한 요구가 커지고 있으나, 현재까지 생활권 수목의 생물적 방제방법은 연구와 적용이 거의 이루어지지 않은 상태이므로 향후 많은 발전 가능성을 가지고 있다.

최근 안전한 먹거리에 대한 관심 제고와 고품질 대추 생산에 대한 관심 급증으로 친환경적인 해충방제법의 필요성이 높아지고 있다. 친환경적 해충방제를 위하여 비가림하우스 별 방충망을 설치하여 해충방제 효과와 과실 생육을 조사하였다. 시험에 사용된 방충방 규격은 각각 25, 18, 6mesh로 대추나무잎혹파리, 장님노린재, 복숭아심식나 방의 차단효과를 보았다. 대추나무잎혹파리의 경우 피해엽율이 1.2%, 10.8%, 14.3%, 무처리구는 15%였으며 장님노린 재의 경우 피해눈율이 6.9%, 5.8%, 20.41%, 28.2%였고, 심식나방은 피해과율이 0.0%, 1.0%, 0.0%, 32.0%로 나타났다. 방충망처리에 따른 대추생육은 처리구와 무처리구에서 차이가 거의 없었다. 대추재배농가의 방충망 도입이 해충 방제에 효과가 큰 것으로 판단되며 문제가 되고 있는 세가지 해충을 모두 방제하기 위해서는 25mesh로 처리하는 것이 가장 효과적인 것으로 판단된다.

해충방제용 미생물 선발을 위해 2018년도에 공원·산림·유기농 근권 토양 87지점을 수집하여 갈색거저리를 이용한 insect-bait법 및 자연조건에서의 감염사충으로부터 곤충병원성 곰팡이를 132균주 분리하였다. 분리된 균주는 점박이응 애, 복숭아혹진딧물에 대한 살충활성 스크리닝을 통하여 각 해충별 살충활성을 보이는 56균주를 선발하였다. 선발된 균주들은 rDNA의 ITS영역을 이용하여 분류동정 하였다. 추가 적용범위 확대를 위해 무잎벌(종령 유충), 거세미나방류 (3~6령 유충)와 같은 지하부 이동성 해충의 방제 가능성을 탐색하기 위해 선발 곤층병원성 곰팡이의 포자 현탁액의 관주처리 방법으로 살충활성을 검정 중이다.

최근 수목 병해충을 방제, 예방하기 위해 나무주사법이 많이 사용되고 있다. 나무주사법은 기상에 상관없이 약제처리가 가능하며, 약제사용량 감소와 천적등 비표적 생물에 대한 영향을 줄일 수 있다는 장점이 있다. 하지만 현재 국내에서 나무주사법은 산림 해충을 대상으로만 등록, 적용되어 있을 뿐, 과수에 등록된 제품은 없다. 과수에 나무주사법이 적용되기 위해서는 농약잔류와 약효에 대한 연구가 필요하다. 본 연구에서는 복숭아(천중도)에 Acetamiprid 10% SL을 나무주사 후, 잔류성과 약효를 조사하여 실용가능성을 확인하였다. 약제가 나무주사된 나무는 복숭아 벚나무깍지벌레(Pseudaulacaspis prunicola)에 대하여 방제효과가 있으나, 가지별로 불균일한 약효와 잔류성을 보여 향후 수형, 흉고직경 등을 고려한 나무주사제의 처리법 최적화에 대한 연구가 지속적으로 필요할 것으로 생각된다.

The continuous emergence of chemical-mediated residual toxicity and insect resistance have enforced the regulation of synthetic pesticides. Synthetic pesticides with novel mode of actions could be developed to overcome these issues, but as an alternative biopesticides with more efficacious control activity could be developed by the advanced technology. In pest management, entomopathogenic fungi have high potential in reducing pest population to an economic threshold, and some of isolates have been commercialized. However still a novel application strategy needs to be considered for successful industrialization. An insight on fungal genes in whole genome and transcriptome levels is necessary to understand the role of genes in pathogenesis and genetic diversity of fungal genes. Herein, we have identified the genetic differences of entomopathogenic fungi using whole genome sequencing of Beauveria bassiana (Bb) and tried to understand the interaction between fungus and insect using RNA-seq. We have obtained the whole genome sequence of Bb JEF-007 using PacBio sequencing technology and compared the transcriptomes of Bb JEF-007 and bean bug, Riptortus pedestris before and after the fungal infection using Hiseq 2000 system.

드론을 이용한 밭작물 병해충 방제기준을 설정하기 위하여 드론의 살포높이(3, 4, 5m), 진행속도(3m/sec, 4m/sec) 에 따른 하향풍 속도, 살포 폭, 약량별 감수지에서 입자크기, 살포조건별 낙하입자수 등 드론의 농약 살포특성을 조사하였다. 또, 배추의 주요해충인 배추좀나방, 파밤나방, 담배거세미나방에 대하여 항공방제용 농약 4종을 대상으로 치사농도와 약량을 검정하였다. 드론 표면별 평균 낙하입자비율은 표면 80.5%, 사면 14.8%, 밑면 4.7%였으며, 살포높이별 평균낙하입자수는 3m=53개/㎠, 4m=40개/㎠, 5m=39개/㎠였고, 진행속도별 평균낙하 입자수는 3m/sec=62개/㎠, 4m/sec는 25개/㎠였다. 약제별 배추좀나방 치사농도와 치사량은 승승장구 (10배, 2㎕), 포워드(15배, 1㎕), 섹큐어(20배, 0.5㎕), 레이서((25배, 0.5㎕)였으며, 파밤나방에 대하여는 섹큐어(20배, 1㎕), 레이서((20배, 1㎕), 담배거세미나방은 섹큐어(20배, 1㎕), 레이서((20배, 0.5㎕)였다. 따라서 배추 주요해충 방제를 위한 농약살포방법은 섹큐어액상수화제, 레이서액상수화제를 20배액으로 희석하여 3m 높이에서 3m/sec속도로 살포시 낙하 입자수 72개/㎠로 해충방제에 효과적이었다.

이중나선형 RNA (dsRNA)를 이용한 유전자 발현 억제기술이 다양한 생명체에서 기능 유전체학을 연구하는 데 이용되고 있다. 이 기술의 원리는 전사후 단계에서 유전자 발현을 조절하는 RNA 간섭에 기인된다. dsRNA를 이용하여 특정 유전자의 발현 억제는 심각한 치사효과를 줄 수 있다. 이러한 분자기작을 해충 방제에 적용하여 특정 dsRNA를 이용한 새로운 살충제를 개발하고 있다. 본 종설은 dsRNA를 이용한 RNA 간섭 원리를 설명하고 이를 이용한 해충 방제를 구현한 여러 예를 살펴본다. 그리고 해충방제를 실현시키기 위해 현재 이 기술이 담고 있는 한계 를 고찰하고 이에 대한 대응 방안을 본 종설에서 제공하고자 한다.

Insect pests have been a serious problem over many years and remain a major threat for food production. Although chemical pesticides are major pest control strategies, use of microorganisms such as entomopathogenic bacteria, fungi, viruses and nematodes have continuously increase last few decades to minimize the use of agrichemicals. According to BBC research, the global biocontrol market was about $2.1 billion in 2011, and this is expected to rise $3 ~ 4 billion by 2017. Over 50 entomopathogens are commercially produced and used augmentatively as microbial pesticides. About 175 biopesticide active ingredients and 700 products have been registered worldwide. Bacillus thuringiensis (Bt), Beauveria bassiana, Metarhizium spp., nuclear polyhedrosis virus and Steinernema spp. are the most popular control agents used in plant protection. Among the microbial control agents Bt products have more than 50% of market share. In Korea, only 13 environmentally-friendly crop protectants were registered for insect pest control in 2015. Market share is very low and has grown slowly. We will discuss how we can expand the market with our techniques.

A fumigant, methyl bromide (MB), has been widely used to control insect pests on various post-harvest agricultural products. Due to its negative side effects, such as ozone deplete and phytotoxicity, MB should be replaced and alternative control technique ought to be developed. Furthermore, organic agricultural products need to be treated with non-chemical methods. To meet these needs, controlled atmosphere and temperature treatment technique (CATTS) has been developed to control insect and mite pests infesting various post-harvest fruits. CATTS uses heat treatment at 46℃with a gradual increase of temperature program under ananoxia condition (less than 1% O2 and 15% CO2). The heating rate at ramping step is crucial to prevent any heat damage to fruits by mimicking natural conditions during summer period experienced by fruits in orchards. The exposure period to the high temperature is minimized by anoxia condition, which prevents any recovery metabolism occurring in the target insect/mite pests after the heat treatment. To develop MB alternative technique for exporting apples, CATTS conditions have been determined against several quarantine insect/mite pest, which are restricted from Korean apple import countries. Target insect pests include Grapholita molesta, Adoxophyes paraorana, Tetranychus viennensis, Conogethes punctiferalis, and Carposina sasakii. In each insect species, differences in susceptibility to high temperature were analyzed in all developmental stages. The highest tolerant developmental stages were adults in T. viennensis, but the fifth instar larvae in G. molesta, C. punctiferalis, C. sasakii, and A. paraorana. Gas conditions of CATTS kept 1% O2 and 15% CO2. After the internal fruit temperature increase from 25℃ to 44℃ during ramping stage (120-150 min), CATTS treatment lasted for 1-2 h. Under 1 h CATTS treatment, 100% mortality was recorded in all assays using more than 3,000 individuals of each species of T. viennensis adults, G. molesta fifth instar, C. sasakii fifth instar, and A. paraorana fifth instar. With 2 h CATTS treatment, 100% mortality was recorded in C. punctiferalis assay using 3,022 fifth instar larvae. Under 1 h CATTS treatment, no fruit damage was recorded in Fuji variety of apples. Treatment cost of CATTS is higher than that of methyl bromide (MB) treatment. However, once CATTS is equipped with nitrogen generator, it would be cheaper than MB in treatment cost. CATTS is a promising post-harvest pest control technique to replace MB treatment. Especially, CATTS would be an ideal control technique to disinfect any pests against organic agricultural products.

Symbiotic bacteria are common in insects. Because symbiotic bacteria are known to intimately affect the various aspects of insect host biology, ideally insects can be controlled by manipulating their symbiont. However, the attempts to control insects through their symbiont have been very limited. The paucity of the insect pest control using their symbiont is most likely due to the poor understanding of the symbiotic interactions between host insect and symbiont, which is attributed to the difficulty in cultivation of insect symbionts. However, the recently established bean bug, Riptortus pedestris, symbiotic system provides good opportunities to study insect’s symbiont in molecular level through their cultivable symbionts. Bean bugs acquire genus Burkholderia cells from environment and harbor them as their gut symbionts in the specialized posterior midgut. The genome of the Burkholderia symbiont was sequenced, and the genomic information has been used to generate the genetically manipulated Burkholderia symbiont strains. After orally administering the mutant Burkholderia symbionts into bean bugs for symbiotic association, the bacterial colonization levels in the host gut and host phenotypes were analyzed. As a result, we have identified novel symbiotic factors necessary for establishing successful association with host. Our recent understandings on the bacterial symbiotic factors demonstrate a great possibility to control the bean bug pest using genetically modified Burkholderia symbiont.

Bacillus thuringiensis (Bt) is a Gram-positive and soil-dwelling bacterium and well known for its ability to produce insecticidal parasporal crystalline protein inclusions, which have attracted worldwide interest for effective pest management. A diseased silkworm by Bt was first discovered by Japanese scientist Ishiwata Shigetane in 1901, and in 10 years, it was re-discovered in Germany by Ernst Berliner, who isolated it as the cause of a disease called Schlaffsucht in flour moth caterpillars. The first commercial product, Bt. kurstaki HD-1 was released to a market in France in 1938. Optimization of mass production for crystal production and cost down enabled the industrialization to be successful, and now many products, such as WP, EC, SC and tablets are used worldwide. In 1976 Robert A. Zakharyan found that plasmids in Bt are involved in the production of crystal proteins and endospores. Pore formation model and signal transduction model were revealed to explain the mode of action of Bt. Works on Bt resistance included a group of receptors of crystal proteins, such as cadherin, APN and ALP. In 1996, a Bt cry gene was integrated to cotton, which successfully reached markets. AtMT technology was used to generate Bt crops. Now the area planted worldwide to genetically engineered Bt crops increased to 66 million hectares. Refuge may be particularly important in slowing the spread of insects resistant to the Bt insecticides. Researchers are trying to increase the insecticidal efficacy of integrated Bt crystal proteins using recent biotechnology.

본 연구에서는 과수의 형태, 거리에 따라 약대가 항상 적정 살포 거리를 유지하며 작업을 진행할 수 있도록 초음파 센서의 신호를 실시간으로 받아 약대를 제어하였고, 또한 약대의 보호를 위하여 진행 방향에 장애물이 존재할 경우 회피할 수 있도록 프로그래밍 하였다. 제작된 시스템으로 현장에서 비 자동제어 상태와 자동제어 상태로 실험을 수행하였다. 실험은 과수의 기둥과 잎 부위에 감수지를 일정간격으로 설치하고 시스템을 이용하여 분사한 후 감수지의 영상을 스캔하여 영상처리를 통해 분석하는 방법으로 이루어졌다. 상 방향 분사 실험에서는 시스템과 대상의 거리가 0.9m~1.1m로 설정해둔 적정거리를 벗어나지 않았기 때문에 비 자동제어와 자동제어 상태 모두 양호한 결과를 보였다. 하지만 측 방향 분 사 실험에서는 비 자동제어 시 우측 열은 98.09%의 분 사율을 보였으나 좌측 열은 69.25%로 낮게 나타났다. 이는 실험이 수행된 배 과원의 경우 과수의 좌측 열이 수평하게 식재되어 있지 않았기 때문으로 비자동제어 상태에서는 좌측열의 과수에 분사되는 양이 줄어들었으나 자동제어 상태에서는 좌, 우측열의 과수에 분사되는 양이 각각 92.66%, 94.64%로 균일하게 나타났다. 시스템 의 제어 속도를 측정하기 위하여 방향 별 약대의 속도를 측정하였고 각각의 속도는 수직방향 100mm/s, 수평 방향 100mm/s, 각 변화 3o/s로 측정되었다. 초기 목적했 던 바와 같이 과수의 형태, 거리에 따라 약대가 적정 거리를 유지하며 작업을 진행함으로 인해서 균일한 살포량을 유지 할 수 있음을 확인하였다.

A postharvest treatment called CATTS (controlled atmosphere and temperature treatment system) has been used as an alternative nonchemical measure for methyl bromide fumigant treatment. This study applied CATTS to control the red flour beetle, Tribolium castaneum, infesting stored grains. Adults of T. castaneum were susceptible to 46℃ heat treatment. The susceptibility was further enhanced by addition of CA conditions (15% CO₂ and 1% O₂). When CATTS (46℃, 15% CO₂, 16℃/h treating rate) was applied to different developmental stages of T. castaneum, it showed 100% control efficacy by 120 min exposure. There was a variation in CATTS susceptibility among developmental stages, in which late instar larvae were most tolerant. Heat shock proteins of T. castaneum appeared to be implicated in the tolerance of CATTS.

In Korea, agricultural environment has been rapidly changed by several factors and the directions of agricultural policy of government have not been maintained by the sudden changes in recent years. The paradigm of agriculture policy is shifted from the maintenance and conservation of agricultural production to the increase of economic, social and ecological value based on agricultural profitability. The agricultural policy and production system are changed by following main social concerns; first the concern of the exhaustion of natural resources, second the quality of life is decreased by the physical and biological change of climate disturbance, third the deterioration of living environment by human activity and finally, the attention of food security by the unbalance and decrease of food production. Furthermore, in spite of low food (23%) and energy (29%) self-sufficiency, it would seem that the importance of the expansion and improvement of food production is not considered by agricultural policy makers. The foundation of agricultural production is collapsed and weakened because the policy makers decide to relegate the importance of food production to lower priority. Our present life style will be changed by the exhaustion of carbon energy. As a result, the production system of agricultural products will be improved and the price of the products will be increased. It is discussed how our agricultural environment will be changed with the surroundings, what factors, specially climate changes, will be considered to research pest management, and how the strategy of green pest management based on sustainable agriculture will be possible.

This study was conducted for establishment of Economic injury levels(EILs) of the tea red spider mite, Tetranychus kanzawai on Rubus coreanus. T. kanzawai was innoculated on May 7 in differently 0, 5, 10, 20 and 40 adults per plant. And pesticide was treated in late April, early May, middle May and late May, respectively. After inoculated of T. kanzawai, the density was increased until the mid-June and decreased gradually in all plots. And higher inoculation density were increased higher than lower inoculation density. Growth variables were not different among experimental plots except number of fruit set, but the number of fruit set and yields were decreased with increasing initial mite density. Densities of T. kanzawai on treated time of pesticide was lower in later treatment time than early treatment time. The late April treatments were not effective of pesticide in harvesting season. The rates of yield loss increased with increasing initial mite density. The relationship between initial T. kanzawai densities and yield losses was well described by a linear regression, Y = 0.6545X + 3.0425, R2=0.9313. Based on the relationship, the number of adults per plant which can cause 5% loss of yield was estimated to be approximately 3.0. And EILs was estimated to be approximately 8.3 adults/leaf in mid-May.

Oriental persimmon, Diospyros kaki Thunb., endemic to East Asia is one of the major fruit crops in Korea. We conducted the faunal survey of mites on persimmon trees in Korea from June to September 2006, especially focusing on herbivorous and predacious mites. Mites of Tetranychidae and Tenuipalpidae were dominantly collected as herbivores, while those of Phytoseiidae and Stigmaeidae were predominant as predators. All identified tenuipalpid mites were Tenuipalpus zhizhilashviliae Reck. Most of the collected tetranychid mites were found to belong to the genus Tetranychus. To clarify the species identity, additional collections of tetraychid mites during summer 2007 on sweet persimmon were made. The mites were identified as Tetranychus urticae Koch. Four phytoseiid species, Neoseiulus womersleyi (Schicha), Amblyseius eharai Amitai and Swirski, Phytoseius (Dubininellus) rubii Xin, Liang and Ke and Typhlodromus (Anthoseius) vulgaris Ehara were collected. Among them, A. eharai was the most dominant species. Seventeen populations of two spotted mites (TSM) were observed 3 times per month from May to October to figure out their fluctuations at the site of individual farmer’s orchard from Sacheon, Sancheong, and Jinju in Gyeongsangnam- do and Gwangyang, Gurye, and Suncheon in Jeollanam-do. Among them, only 2 sites were properly managed, 5 sites were required to control but the farmers had little information on the mite and its damage, though 10 orchards were not in jeopardy. Number of TSM reached more than 400 at its maximum when 100 leaves were randomly observed at orchards from Sacheon, Okgok, and Muncheok, showing remarkably discolored leaves. For the control of TSM in fields by chemical means, it was tried to select an effective miticides in persimmon fields. Control activity of spiromesifen 20SC showed 99.0% and 98.1% and the activity of acequinocyl 15SC showed 90.8% and 99.0% in Jinju and Sacheon at 20 days after treatment, respectively. It was tried to understand the cause of the fluctuations of TSM populations on the viewpoints of pesticide spray, density of predacious mites, rainfalls, and weeds in the persimmon orchards. Various factors considered to contribute to the cause of population fluctuations, depending upon the situations of each orchard. To develop as a potential resource of biological control agents, it was tried to find out winter spatial distribution and movement of Amblyseius eharai (Acari: Phytoseiidae) on persimmon trees in Korea using artificial materials. We attached Phyto traps and urethane foam on persimmon trees in early November 2007 in Sacheon, Korea to estimate overwintering ecology of the predominant phytoseiid species Amblyseius eharai on persimmon. Most of A. eharai were found on the samples of branches, such as pedicel. In early spring, A. eharai was abundantly collected in the weekly and long-term traps before the leaf extension of persimmon trees, which additionally enhanced the possibility that A. eharai overwintered on the trees.