Plant specific gene family, NAC (NAM, ATAF, and CUC) transcription factors have been characterized for their roles in plant growth, development, and stress tolerance. In this study, we isolated OsNAC69 gene and analysed expression level by inoculation of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo). NAC transcription factor family can be divided into five groups (I–V). On the basis of phylogenetic analysis, OsNAC69 was fall into group II. OsNAC69 was strongly induced 1 hr after infected with Xoo. To investigate its biological function in the rice, we constructed vector for overexpression in rice, and then generated transgenic rices. Gene expression of OsNAC69-overexpressed transgenic rice lines were analyzed by northern blot. Analysis of disease resistance to pathogen Xoo, nine OsNAC69-overexpressed transgenic rice lines showing high expression level of OsNAC69 were shown more resistant than wild type. These results suggest that OsNAC69 gene may play regulatory role during pathogen infection.

The antimicrobial peptide possesses defence system to virus, fungi and bacteria. To study antibiotic in plant, antimicrobial peptides were obtained by PCR analysis by primers designed from antimicrobial peptides (Gene bank accession no. NM-004345), cloned in pET28 expression vector and the vector transformed into E. coli. And this gene was inserted into Ti-plasmid VB2 vector, which contained the pGD1 promoter. The expression construction was transformed into Agrobacterium EHA105 and then plant tissues of rice (Oryza sativa). Seeds from transgenic plants (T0) were germinated on selective media containing spectinomycin 50 mg/L. Selected plants and wild type were analyzed by PCR and RT-PCR with pGD1 promoter region and transgene specific primer set. All transgenic plants showed expression pattern of similar levels. We showed that the chromobody is effective in binding GFPand antimicrobial peptide gene in tobacco leaf. Most interestingly, this can be applied to interfere with the function of GFP fusion protein and to mislocalize (trap) GFP fusions to the plant cytoplasm in order to alter the phenotype mediated by the targeted proteins. Bacterial blight disease was enhanced resistance in transgenic lines. These results showed that antibiotic peptides might show a broad-spectrum antimicrobial activity.

Japonica rice cultivars exhibit high susceptibility to bacterial blight(BB) disease due to genetic vulnerability in Korea. Korean japonica resistant rice cultivars mainly possess one of the genes, Xa1 or Xa3 for BB resistance. These resistance genes are becoming susceptible to K3a, resulting in serious rice yield reduction. This study was carried out to confirm the effect of xa13 gene pyramiding for developing of japonica rice cultivars resistant to BB pathogen breaking down Xa1 or Xa3. IRBB4 conferring Xa4 gene was resistant to K1(HB01013), K2(HB01014), K3(HB01015), and moderately resistant to K3a(HB01009). IRBB5 having xa5 gene was resistant to K1, K2, K3, and K3a. The recessive gene xa13 was resistant to K1 race but susceptible to K2, K3, and K3a. But Xa21 gene is susceptible to predominant K1 race but resistant to other races such as K2, K3, and K3a. Two genes Xa3 and xa13 were susceptible and Xa4 gene was moderate resistant to 24 isolates. xa5 and Xa21 genes were resistant to all isolates including K3a. When xa13 gene combined Xa4, xa5 and Xa21 genes, effect of xa13 gene pyramiding showed higher resistant reaction than that having singe gene out of Xa4, xa5, and Xa21. The order of resistance against 24 isolates breaking down Xa3 gene was IRBB55(xa13+Xa21) > IRBB53 (xa5+xa13) > IRBB51 (Xa4+xa13).

Near-isogenic lines (NILs) carrying bacterial blight resistance genes (Xa4, xa5 and Xa21) were developed in japonica rice using Suweon345 as genetic background. NILs were selected by gene specific DNA markers and inoculation of K1 or K3a race. NILs conferring Xa4 were resistant to K1, K2, K3, and moderately resistant to K3a. NILs conferring xa5 were resistant to K1, K2, K3, and K3a. NILs having Xa21 were susceptible to K1, while resistant to K2, K3 and K3a. Target genes of NILs with the genetic background of Suweon345 were also confirmed by using eleven Philippines races and International Rice Bacterial Blight (IRBB) NILs carrying Xa4, xa5 and Xa21. All NILs had no significant difference from their recurrent parents in the major agronomic traits except for panicle length and brown rice 1,000 grain weight. Heading date of NILs ranged from Aug. 10 to Aug. 11, which was similar to that of recurrent parent, Suweon345. Culm length, number of grains per panicle and ratio of ripened grain of NILs were similar to those of Suweon345. Milled rice of NILs was ranged from 4.82 to 4.93MT/ha. These NILs will be useful for improving resistance to K3a race of bacterial blight pathogens in Korean japonica cultivars.

식물 특이 NAC (NAM, ATAF, and CUC) 전사인자는 식물 성장, 발달과 스트레스에 대한 저항성에 관여한다고 알려져 있다. 본 연구에서는 벼의 NAC 전사인자 중의 하나인 OsNAC69 유전자를 분리하였으며 유추된 아미노산 서열을 바탕으로 조사해본 결과 이 유전자는 NAC 전사인자의 5개 group 중에서 group II에 속하였다. 흰잎마름병균인 X. oryzae pv. oryzae (Xoo)를 처리하여 발현을 분석한 결과 접종 1시간 이

Bacterial blight(BB) caused by Xanthomonas oryzae pv. oryzae(Xoo) is one of the most economically destructive bacterial diseases of rice in worldwide. Utilization of resistant cultivars carrying resistant gene(s) is relatively an effect method to control this disease. About 34 resistant genes for BB resistance have been identified in many countries. Among them, Xa1 and Xa3 genes against bacterial blight have been incorporated into improved korean japonica rice varieties. Now, Ilmi carrying Xa1 gene and severial cultivars carrying Xa3 gene are widely grown in our country. In recent year, xa5, Xa21 and Xa23 genes are using in rice breeding programs for japonica resistant cultivars to bacterial blight. Resistant cultivars incorporated with a diverse single gene and two, three, or the more major gene necessite in the future.

벼 흰잎마름병은 세계적으로 벼 재배치에서 가장 문제시되는 병해충의 하나이다. 우리나라의 경우 상습발생지를 중심으로 Xa1과 Xa3 이 저항성 유전자로 활용되었으나, 소수의 저항원이 집중적으로 활용됨으로 인해 최근 이병화가 급속히 진행되고 있다. 특히 최근 Xa1과 Xa3 모두를 침해하는 새로운 균계 K3a가 확인됨에 따라 새로운 저항성 유전자의 동정 및 활용의 중요성이 높아가고 있다. 국내육성 자포니카품종 화성벼와 야생벼 O. minuta 간의 종간교잡을 통해 확립된 수원506호의 흰잎마름병에 대한 유전분석을 실시하였다. 수원506호와 통일계 품종인 밀양23호간의 교잡을 통해 확보한 F2 개체들을 활용하여 흰잎마름균주 HB3011 의 접종에 따른 병반장의 변이와 유전자지도 작성에 사용된 SSR 마커의 유전자형간의 연관성분석을 수행하였다. 수원506호의 흰잎마름병 저항성을 지배하며 우성유전자로 작용하는 주동유전인자가 염색체 4변 하단에서 SSR 마커 RM255 에 의해 표지 되었는데, 해당 염색체영역은 Xa1과 Xa2 및 Xa22 등이 보고되었던 영역과 매우 유사할 것으로 추정되었다.

야생벼은 재배벼의 친환경적성을 강화시킬 수 있는 병해충 저항성 및 불량환경에 견딜 수 있는 유용한 유전자들의 보고로 알려져 왔다. 국내에서 육성된 벼 품종인 '화성'(AA게놈)와 야생벼인 Oryza. minuta(BBCC 게놈; Acc.=101141)간의 교잡을 통하여 종간잡종 후대들이 육성되었다. 불화합성과 초기분리세대의 극심한 불임을 극복하기 위해 배주배양으로 F1 개체를 확보하였으며, '화성'으로의 여교잡을 수 차례 실시하였다. 확립된 계통들에 대

Currently, more than 33 resistance gene against bacterial blight(BB) have been identified and used to develop resistant cultivar. IRRI developed near isogenic lines carrying monogenic resistance gene and pyramided genes, IRBB lines. This study was conducted to identify the response of resistance gene to Korean bacterial blight races(K1, K2, K3, and K3a) in IRBB lines. Xa1(IRBB1) was resistant to K1 race and Xa2(IRBB2) was resistant to K1 and K2 race. Xa3(IRBB3 and IRBB203) exploited in many breeding programs in Korea were resistant to K1, K2, and K3 race but susceptible to K3a. Xa4(IRBB4 and IRBB204), xa5(IRBB5 and IRBB205), Xa7(IRBB7 and IRBB207) were resistant to four races. xa8(IRRR8) and Xa11(IRBB11 and IRBB211) showed same reaction of Xa2, resistant to K1 and K2. Xa10(IRBB10 and IRBB210), xa13(IRBB13), Xa14(IRBB14 and IRBB214) showed same reaction of Xa1, resistant to K1. Xa21, identified from the wild rice, Oryza longistaminata, is highly resistant to a broad spectrum of Xoo races. In this study, Xa21 was resistant to K2, K3, and K3a but susceptible to K1 widely spreading in Korea. So, Xa21 may be vulnerable to Korean bacterial blight. But pyramided lines with Xa21 showed high level of resistance to all races of Korea. Pyramided lines with Xa4 which conferred durable resistance in many commercial rice cultivar, xa5 which provides recessive resistance, and other resistance genes showed high level of resistance to Korean bacterial blight races.

Korean Japonica resistant rice cultivars mainly posses one of the genes, Xa1 or Xa3 for BB resistance. These resistance genes are becoming susceptible to K3a, resulting in the breakdown of resistance of Japonica cultivars. Especially, glutinous rice cultivars exhibit high susceptibility to bacterial blight(BB) in Korea. This study was carried out to develop glutinous Japonica near-isogenic lines(NILs) conferring useful single gene such as Xa2, Xa3, xa5, Xa21, etc. Six NILs conferring Xa2, Xa3, xa5, xa8, Xa14 and Xa21 genes were developed. Six NILs with Sangjuchalbyeo genetic background were selected by DNA markers and inoculation of K1, K2, K3 and K3a race. NILs having Xa14 was resistant to K1 and susceptible to K2, K3 and K3a. NILs with Xa2 and xa8 was resistant to K1, K2 and susceptible to K3 and K3a. NIL with Xa3 was resistant to K1, K2, K3, but susceptible to K3a. NIL with xa5 was resistant to K1, K2, K3, and K3a. NIL having Xa21 was susceptible to K1, while resistant to K2, K3 and K3a. Identification of xa5 and Xa21 genes were carried out by STS markers. Heading date of NILs ranged from Jul. 31 to Aug. 6. Culm length, No. of grains per panicle and ratio of ripened grain of NILs were a little different to those of Sangjuchalbyeo. Milled rice of NILs were ranged from 437kg/10a to 463. Agronomic traits of NILs containing Xa2, Xa3, xa5, xa8, Xa14 and Xa21 were similar or a little different to those of recurrent parent. These NILs would be useful to develop durable resistant glutinous varieties in Korea.

"진백"은 국립식량과학원 벼맥류부에서 2008년도에 육성한 중만생 고품질 벼흰잎마름병 신균계, K3a 저항성 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1. 남서해안지, 호남 및 영남평야지 보통기 보비재배에서 평균 출수기가 8월 18일로 남평벼보다 1일 늦은 중만생종이다. 2. 간장은 71 cm로 '남평벼'에 비해 8 cm 정도 작고 주당 수수는 남평벼와 비슷하며 수당립수는 적고 등숙비율이 다소 높은 중립종에 속한다. 3. 위조현상은 나타나지

벼흰잎마름병 저항성 유전자 Xa3을 침해하는 K3a 균계를 포함하여 24개의 균주에 대한 단일저항성 유전자와 2개 이상의 주동저항성 유전자가 결합된 계통에 대한 결과를 요약하면 다음과 같다. 1. 벼흰잎마름병 저항성 유전자 Xa3, Xa4, xa5 및 Xa7은 K1, K2, K3 균계에 저항성 반응을 보이며 K3a 균계에 대하여 Xa4는 중도저항성, xa5 및 Xa21은 저항성반응을 보였다. 2. 벼흰잎마름병 저항성 유전자 Xa3을 침해하는 24개 균주

‘Gangbaek’ is a japonica rice variety developed and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2006. ‘Gangbaek’ was derived from a cross between ‘Suweon345’ with good grain quality and ‘DV85’ resistant to bacterial blight, K3a. F1 plants were grown in the greenhouse in winter of 1992/1993 and backcrossed with ‘Suweon345’ as the recurrent parent. Plants resistant to K3a race of bacterial blight (BB) were selected from BC1F1 to BC4F1 and used as parents in the backcrossing processes. This variety has about 120 days growth duration from transplanting to harvesting in west-southern coast and Honam plain of Korea. It is about 69 cm in culm length and tolerance to lodging. In reaction to biotic and abiotic stresses, it shows moderately resistance to blast, and resistance to bacterial blight pathogen, K1, K2, K3 and K3a, but susceptible to other major diseases and insect pests. The milled rice of ‘Gangbaek’ exhibits translucent, relatively clear non-glutinous endosperm and midium short grain. It has lower amylose content of 18.6% and protein content of 6.4% compared with ‘Nampyeongbyeo’. The milled rice yield performance of this variety is about 5.28 MT/ha in local adaptability test for three years. This cultivar would be adaptable to the bacterial blight-prone area in the south-western coastal and Honam plain of Korea.