고등식물에 있어서 엽록체에 존재하는 저 분자량 heat shock protein (smHSP)은 식물의 내열성 획득에 있어서 필수유전자임이 mutant를 이용한 유전학적인 연구에 의해 보고된 바 있다. 고온내성이 강한 작물인 벼로부터 엽록체 smHSP cDNA를 분리하고자 벼의 잎에서 분리한 mRNA로 작성한 cDNA library로부터 screening하였다. 선발된 smHSP cDNA는 1,026 bp의 염기로 구성되어 있었으며, 239개의 아미노산

사람 성장호르몬 수용체(hGHR) cDNA는 PCR방법에 의하여 fagment로서 보고되어진 바 있으나, liver cDNA로 부터 전장을 cloning한 보고는 없는 실정으로 본 연구에서는 기능을 가진 약 4.6kbp의 cDNA hGHR을 cloning 하는데 성공하였다. 먼저 cloning하기 위하여 human liver mRNA와 human breast cancer tissue로부터 회수한 mRNA를 RT-PCR방법에 의하여 human cDNA l

To investigate the involvement of prepro-proopiomelanocortins (POMCs) with hypermelanosi on the blind side of olive flounder, Paralichthys olivaceus, we surveyed tissue sites in which the three POMCs genes are expressed, and compared pituitary-ofPOMCs (ofPOMC-Ⅰ, Ⅱ and Ⅲ) mRNA activities between the normal and the hypermelano flounders. For the studies, we refereed to the information of ofPOMC-Ⅰ and Ⅱ genes in NCBI, but we try to newly discover ofPOMC-Ⅲ sequence. At first, we cloned and characterized cDNA encoding its ofPOMC-Ⅲ from the pituitary of olive flounder The ofPOMC-Ⅲ cDNA consists of 1,258 bp nucleotides and 7 peptides (Signal peptide, N-POMC, α-MSH, CLIP, N-β-LPH, β-MSH and β-END-like peptide) encoded in 231 amino acids. In the test of tissue distribution of ofPOMCs in olive flounder, we found that ofPOMC-Ⅰ is expressed in pitutary only, but ofPOMC-Ⅱ and Ⅲ are expressed in all tissues examined. In the comparison of ofPOMCs mRNA expression between the normal and the hypermelano fish, expression of ofPOMC-Ⅰ, Ⅱ mRNA was significantly higher in the hypermelano group than in normal group, but ofPOMC-Ⅲ was not significantly different between two groups.

고려 인삼(Panax ginseng)의 뿌리로부터 ribulose-1,5-bisphosphate carboxylase small subunit(rbcS) 유전자를 선발하여 sequence 분석을 실시하였다. 고려 인삼 rbcS cDNA는 790 bp 염기로 구성되어 있으며, 183개의 아미노산(pI 8.37)을 코드하는 549 bp의 ORF를 가지고 있고 단백질의 분자량은 20.5 kDa으로 추정되었다. 인삼 rbcS는 기존에 보고된 것과 유사성을 나타내었으며, Helianthus annuus(CAA68490)에서 분리된 것과 78%의 높은 상동성을 보였다. 기존에 데이터베이스에 축적되어 있는 다른 식물체로부터 분리된 rbcS와 아미노산 서열을 비교한 결과 인삼의 ybcS는 H. annuus (CAA68490), C. morifolium (AAO25119), L. sativa (Q40250)와 밀접한 유연관계에 있는 것으로 조사되었다.

A class I type 2 metallothionein (CMet2) cDNA from taproot of Codonopsis lanceolata was isolated and characterized. A CMet2 cDNA was 572 nucleotides long and had an open reading frame of 234 bp with a deduced amino acid sequence of 78 residues (pI = 4.99). The deduced amino acid sequence of CMet2 matched to the previously reported type 2 metallothionein-like protein genes and showed 74% identity with that of G. max (BAD18377) and C. arietinum (CAA65009). Expression of CMet2 by the RT-PCR was increased at 1 hr after cadmium and hydrogen peroxide treatment, respectively.

A full-length cDNA (PPrx1) encoding peroxidase has been isolated and its nucleotide sequence determined from flower bud in ginseng plant (Panax ginseng). A PPrx1 cDNA is 1192 nucleotides long and has an open reading frame of 1062 bp with a deduced amino acid sequence of 354 residues (pI 7.53). The deduced amino acid sequence of PPrx1 matched to the previously reported peroxidase protein genes. The PPrx1 showed a high similarity with the 64% identity with peroxidase of N. tabacum (AAK52084). In the phylogenetic analysis based on the amino acid residues, the PPrx1 was closer with peroxidase of G. max (AAD37376).

Ribosomal protein complex with ribosomal RNA to form the subunits of the ribosome serve essential functions in protein synthesis. A full-length cDNA (PRPS4) encoding ribosomal protein S4 has been isolated and its nucleotide sequence determined in ginseng plant (Panax ginseng). A PRPS4 cDNA is 1105 nucleotides long and has an open reading frame of 792 bp with a deduced amino acid sequence of 264 residues (pI 10.67). The deduced amino acid sequence of PRPS4 matched to the previously reported ribosomal protein S4 genes. Their degree of amino acid identity ranged from 68 to 92%. Phylogenetic analysis based on the amino acid residues showed that the PRPS4 grouped with ribosomal protein S4 of S. tuberosum (CAA54095).

A gene coding for the GST of cotton (Gh-5) was cloned into Escherichia coli and experssed. The enzyme remained within the cytoplasm of E. coli. An 696 bp open reading frame was in the 988 base pair fragment of the recombinant plasmid pET-30b(+). The deduced protein sequence consists of 232 amino acids and has a molecular mass of 30235.58 Da. The cloned enzyme conjugated reduced glutathione and 1-chloro-2,4-dinitrobenzene (CDNB). Plant GST cDNA was expressed in microbe and produced polypeptide had function as an enzyme.