This study was conducted to investigate nuclear remodeling and developmental rate following nuclear transfer of fetal fibroblast cells, ear skin cells and oviduct epithelial cells into porcine recipient oocytes. To test par-thenogenetic activation, oocytes were treated with a 6-dimethylaminopurine (6-DMAP), a single DC-pulse (DC), calcium ionomycin (ionomycin), DC+6-DMAP and ionomycin + 6-DMAP after in vitro maturation. For nuclear transfer, in vitro matured oocytes were enucleated, and donor cells were transferred into oocytes. Cloned embryos were fused and stimulated with 6-DMAP for 4 h and cultured in vitro for 6 days. Among treatments for parthenogenesis, the activation rate of DC +6-DMAP treatment was significantly higher than that of single treatment roups (p<0.01), except for DC treatment group. However, the difference was not significant in activation rate compared to other complex treatment groups. Nuclear swelling of the cloned embryos was initiated at 60 min after stimulation and increased afterwards. Fusion rates were not different among different donor cells. Cleavage rates of DC treatment groups were significantly higher than those of DC+6-DMAP treatment groups (p<0.05) in case that fetal fibroblast and ear cells were used for nuclear donor. The cloned embryos from developed to blastocysts in oviduct epithelial cell nuclear transfer with DC+6-DMAP treatment was significantly higher compared to those with DC only treatment (p<0.05). However, no blastocyst was developed from nuclear transfer of fetal fibroblast and ear cells regardless of activation treatments. Based on these results, a proper activation stimulation may be necessary to increase the activation rate and the development to blastocyst in cloned porcine embryos.

The $18^{th}$ century's Industrial Revolution brought about influx of commerce, industry, and agricultural population into the urban areas, entering the $19^{th}$ century. Quality of rural communities declined, and the exploding population in the cities gave rise to various problems. 'Mietskaserne' Blockhousing was constructed by the 'Hobrechtplan', but led to social problems such as poor living conditions, insufficient sunshine due to overcrowding, slums due to incomplete urban infrastructure, epidemics, and so on. Starting in the early $20^{th}$ century, Berlin has driven forward a remodeling plan under the motto of 'critical reconstruction (kritische Rekonstruktion)'. It is performed in the place, which represents the vicissitudinous history of Berlin with site plans coexisting past with presence, using modern vocabulary of architectural forms. Reconstructing a city is a process which not only raises the economic value of each building consisting a city, but also a redevelopment process that brings out cultural value of an era. When a new era emerges buildings get reconstructed or rebuilt, and thereby form the identity of a city by reflecting its society, culture, politics, economy, and history. Old German architecture were not destroyed or rebuilt recklessly just by the fact that they are functionally or aesthetically outdated. Each building is treated as precious cultural heritage reflecting the history. This is how Berlin is being transformed today.

This study researched problems of safety inspection method and current legislative system for the structure safety evaluation of Rahmen structure affected by remodeling. The elements of weight increase were examined in terms of differences of load moment, shear force, compressive stress and amount of steel before and after remodeling by structure analysis. The thorough examination for impacts of weight increase is indispensable to change of use or extension.

본 연구에서는 노후화된 공동주택 수직 증축 리모델링 시 면진시스템의 적용성을 검토 및 면진시스템 적용을 위한 기초자료를 제공할 목적으로 동일한 구조로 수직 증축 시 수직 층수와 면진주기에 따른 최적 면진주기를 산정하였다. 해석결과, 3개층 수직 증축 시에는 면진주기를 비면진 건물 주기의 2배 이상, 2개층 수직 증축 시에는 비면진 건물 주기의 3배 이상을 확보하여야만 충분한 면진효과를 나타내는 것을 알 수 있었다. 또한, 1개층 수직 증축 시에는 비면진 건물 주기의 4배 이상을 확보하여야만 면진효과를 나타내었다. 위의 결과를 활용하여 3개층 수직증축 리모델링 대상 공동주택에 면진시스템을 적용하여 그 효과를 검증하였다. 최상층 최대응답가속도가 비면진 건물의 최대응답가속도 보다 X방향의 경우에는 약 70%로, Y방향의 경우에는 약 65%의 감소를 나타내었다. 또한, 밑면 전단력의 경우, 비면진 건물과 비교하여 X방향 및 Y방향 모두 약 30% 감소하는 것으로 나타났다.

주택법에 근거하여 철근콘크리트 공동주택의 리모델링 시 최대 3개층의 수직증축 및 기존 세대수의 15%까지 증축이 가능하게 되 었다. 수직증축 리모델링 가능 여부는 안전진단 기준 및 매뉴얼을 바탕으로 기울기 및 침하, 내하력, 내구성 평가 부문에 대한 조사를 통하여 평가하고 판정하도록 되어 있다. 그러나 현행 증축형 리모델링 안전진단 기준 및 매뉴얼은 재건축 안전진단 기준과 비교하여 조사대상표본 수를 증대시키는 등 보수적인 것으로 평가되고 있으며, 특히 내구성 평가 부문에 검토 및 개선이 필요한 것으로 생각된다. 따라서 본 연구에서는 수도권 지역 공동주택의 증축형 리모델링 안전진단 조사 사례에 대한 내구성 조사 항목 중 콘크리트 탄산화를 대상으로 통계적인 수법에 의해 표본 수 합리화 방안을 제시하였다.

The 'Housing Act (‘14.04), which permits the remodeling of old apartment buildings, has been implemented. In particular, vertical remodeling is generally implemented by adding 2~3 floors above the top floor of existing apartments. Technically, it is necessary to reinforce the existing pile due to the increase of vertical, horizontal, seismic load, etc. However, the existing piles are loaded simultaneously and so each pile was constructed at limited base space for application of reinforcement method. The bearing capacity of existing piles decreases due to vertical expansion so the bearing capacity of the piles must be confirmed by the indirect method(laboratory test etc.) in the first safety diagnosis and by the direct method(field test etc.) in the second safety diagnosis.

Most of the apartment buildings to be remodeled are in the form of wall structures, and walls are very important structural members. When remodeling, there are cases where existing walls are demolished and new walls are installed. If a wall is newly installed, the continuity of the wall from the foundation to the top floor must be ensured. Some slabs prevents the vertical continuity of the reinforced wall. It is because the upper and lower wall reinforcing bars must be connected through the demolished slab and the concrete must be poured. Therefore, in this study, the method to secure the vertical continuity of the reinforced wall during the remodeling will be examined.

Standard reinforcing technology of remodeling will be established through the actual remolding cases. Reinforcement techniques can be classified as a technique of seismic retrofit technologies, vertical and horizontal expansion joint detail, footing reinforcing technology, and general repair method.

In this study, existing wall-type apartment in 1990s is evaluated by KBC2009. Based on structural calculation, seismic performance evaluation is performed with dynamic nonlinear analysis. After that, this building is extended up to three stories in vertical direction. In this study, the seismic performance evaluation results of wall-type apartment can be used in performance-based design of the vertical extension remodeling.

The recent revision of Housing Act allow vertical extension up to maximum 3 stories and increase the ratio of floor area to site up to 15%. Currently, the feasibility of performing vertical extension is evaluated based on safety diagnosis provisions and manuals for vertical extension with preliminary investigations on slope, deflection, load-bearing capacity, and durability. However, a need for more reasonable evaluation methodology for the preliminary investigation is still required because the current procedures are borrowed from safety diagnosis provisions and manuals for reconstruction without detailed examinations on evaluation criteria and sampling methods. Accordingly, this study is intended to suggest a method to obtain feasible sampling numbers for durability assessment by analyzing field data on concrete carbonation, chloride content, and steel corrosion. The results of this study are expected to be beneficial for establishing more reasonable field sampling number, and in turn, more reliable durability assessment protocol for vertical extension.

In the recent remodeling construction for old apartment, it is general to extend the parking space for the increased cars for residents. This paper reviews the extension methods of parking space and presents the problems that must be solved for the construction. In this paper, three extension cases of parking space in the remodeling projects were reviewed. As a result, the demand for the extension needs to be analyzed for the various cases and the technologies for the best-fitted construction should be suggested based on the demand.

Post-translational modifications of nucleosomal core histones play important roles in biological processes via altering chromatin structure and creating target sites for proteins acting on chromatin. Molecular genetic studies with Arabidopsis have verified several epigenetic factors that regulate flowering time. However, the roles of chromatin remodeling factors have not been well explored in rice. Here, we identified chromatin remodeling factors, OsVIL1, 2, and 4 (Oryza sativa VIN3-LIKE) genes, that regulate grain yield. OsVIL proteins contain a plant homeodomain (PHD) finger, which is a conserved motif of histone binding proteins. We showed that plant height and number of spikelets per panicle were increased in the OsVIL2-overexpression (OsVIL2-OX) and osvil4 plants, respectively. Each mutants (OsVIL2-OX and osvil4) exhibited longer internodes and thicker stems than wild type controls. Histochemical analysis revealed that cells are smaller in OsVIL2-OX and osvil4 plants. We performed an RNA-seq using 1st internodes of WT and OsVIL2-OX stems and got the suppressed target genes in the OsVIL2-OX. OsCKX2, which encodes cytokinin oxidase/dehydrogenase is one of the suppressed genes in the OX plants and we verified decrease of that gene using qRT-PCR and closed chromatins of OsCKX2 were enriched in the OX plants by using ChIP. As results of these, cytokinins were enriched in the OX plants. These demonstrate that OsVIL2 and OsVIL4 antagonistically regulate plant height and number of spikelets by controlling cytokinin contents. Like OsVIL2-OX and osvil4 plants, besides, OsVIL1-OX plants were also shown increased plant height and biomass. We propose that OsVILs may be used for improving grain yield by increasing biomass.

세계적으로 에너지나 자원의 절약 및 환경에 대한 관심이 높은 시점에서 건축물의 재생은 환경, 생태적 측면의 접근뿐만 아니라, 기존의 자원이나 자산의 보존·재생에 의한 문화계승이라는 측면에서 높게 평가받고 있다. 본 연구는 다양한 재생 건축물의 사례 연구를 통해 기존 용도의 변경여부, 기존 건축물 내·외부 보존 정도 및 방식, 공간 형태 및 프로그램 변경 등 다각도의 분석을 실시하고, 이를 바탕으로 향후 건축물 리모델링의 계획 방향 설정 시 참고 가능한 기초자료를 구축하는 것을 목적으로 한다. 연구의 결과를 살펴보면 다음과 같다. 첫째, 용도변화는 기존 건물의 원용도를 새로운 용도로 변경하는 ‘전용형’이 6사례(75%)로 나타났고, 나머지는 원용도를 이어서 사용하는 ‘계속형’이었다. 전용형의 경우 원용도는 창고, 가압장, 공원 관리사무소로 다양하지만 모두 공공적 성격의 문화공간으로 변경되었다. 둘째, 공간변화의 유형과 공간 리모델링 시 원형의 보존 및 개조 수준을 조사한 결과, 외관은 부분개조를 하되 사례별로 원형의 형태, 재질, 색상 등의 보존 정도가 달랐다. 내부 역시 부분개조가 대부분이었고, 구조적, 기능적 보강이 필요한 부분에만 새로 보수를 하고 기둥, 벽면, 천장 등의 원형을 최대한 디자인에 반영하는 형식이었다. 셋째, 재생을 통해 획득할 수 있는 가치요소 중 경제적 가치는 모든 사례에서 나타난 반면, 역사성과 기능적 가치는 사례별로 차이를 보였다.

In terms of both quality of rural lifestyle and cultural awareness, the importance of community facility is being emphasized. Nevertheless, by occasion of graying, population outflow and revenue loss, rural society faces the erosion of community. For these reasons, government is promotion an community space construction project on rural areas which is lack of community facilities. Whereas the projects are just one off thing not include upkeeping, so lots of functional overlapping community facilities are formed in same place. In this study has classified rural community facility remodeling element and designed rural community facility evaluation system of each elements. In order to implement of purpose, it has categorized community remodeling index in rural area by literature analysis and site survey. For setting remodeling rating system, this study analysed preceding research data and conducted a Delphi survey of 30 experts. As a result, this study deducted 29 categories of community remodeling index and 21 categorizes of remodeling rating clause. Taking this result, this study progressed case study on seonheul-ri in Jeju. Implications, limitations and future research directions are presented. It would be groundwork for remodeling system development in rural community facility.