Transplantation of stem cells, such as mesenchymal stem cells (MSCs), is a promising strategy for treating several types of intractable disorders. Mechanistically, it could not only replace damaged cells by direct contribution, but also establish an anti-inflammatory or immunomodulatory microenvironment. However, the cellular mechanisms underlying molecular and biological properties of stem cells during ex vivo expansion and also after transplantation in pathological environments remain largely elusive. We recently developed the cyanoacrylamide-based coumarin derivatives (Fluorescent real-time thiol tracer; FreSHtracer*) reversibly react with glutathione for monitoring of glutathione levels in living stem cells. These probes revealed that glutathione levels are heterogeneous among subcellular organelles and among individual cells and show dynamic changes and heterogeneity in repopulating stem cells depending on oxidative-stress or culture conditions. Importantly, a subpopulation of stem cells with high-glutathione levels exhibited increased self-renewal and migration activities in vitro and showed improved therapeutic efficiency in treating asthma. Furthermore, employing a novel combination of longitudinal intravital confocal fluorescence imaging and microcystoscopy in living animals, we investigated the distributions and properties of transplanted multipotent MSCs derived from human embryonic stem cells at single-cell resolution in real-time by performing confocal imaging of bladder tissues in a rat model of IC/BPS for up to 6 months post-transplantation. These novel real-time monitoring strategies demonstrate the novel molecular insight for maintaining stem cell functions and also enhance understanding of the in vivo behaviors of the engrafted stem cells, which is crucial to determine the efficacy and safety of stem cell-based therapies. This strategy may facilitate the translation of various stem cell-based approaches into clinical practice.

Honey bee, Apis mellifera L., have been widely used as a model organism for biological science because of its highly developed sociality, specialized labor division and passive population management. In order to examine the expression patterns of genes putatively involved in social development in honey bee, quantitative real-time PCR (qRT-PCR) that has been widely used to investigate the expression level of target gene can be used in honey bee study. However, the selection and validation of optimal reference genes is a crucial step prior to running qRT-PCR. In the present study, therefore, the seasonal expression stability of five candidate reference genes in the abdomen of forager and nurse was investigated using three programs (geNorm, NormFinder and BestKeeper), and selected reference genes were validated by the normalization of expression level of vg encoding vitellogenin. Although three programs revealed slightly different gene stability values, overall the combination of two genes (rpS18 and gapdh encoding ribosomal protein S18 and glyceraldehyde-3-phosphate dehydrogenase, respectively) was resulted in the most suitable use for normalization of the target gene in forager. However, a single gene, either rpL32 or rpS18 in the nurse or either rpL32, rpS18, or gapdh in the comparison between foragers and nurses, were suggested to be applied for normalization of seasonal and labor-specific gene expression by qRT-PCR.

The fruit fly, Drosophila melanogaster, is a good model organism in various areas of biological science. Since D. melanogaster has been thought to be adapted to the chemical stress environment caused by the overripen, decay and fermented fruits, identification of the genes involved in chemical tolerance and investigation of their expression patterns are essential for better understanding of the physiological evolution in D. melanogaster. For investigation of the gene expression level, quantitative real-time PCR (qRT-PCR) can be applied to quantify gene expression level and selection of reliable reference gene(s) for normalization is an accurate step. In the present study, therefore, we validated the expression stabilities of ten candidate reference genes using three softwares (geNorm, NormFinder and BestKeeper) in D. melanogaster exposed to different concentrations of acetic acid, ethanol and 2-phenylethanol. Although three programs resulted in slightly different gene stability ranks, but overall tbp encoding TATA box binding protein was most stable gene in acetic acid and ethanol exposed fly, while nd encoding NADH dehydrogenase was the most suitable reference gene in the case of 2-phenylethanol treatment. In the comparison of three chemical treatment condition, nd was also suggested to be most optimal reference gene. In addition, optimal number of reference gene for accurate normalization was calculated by geNorm pairwise analysis, and selection of multiple reference genes was suggested to be better for target gene normalization method than use of a single reference gene.

과일이나 농작물의 부패 및 발효 환경에서는 Methanol, Ethanol, Acetic acid을 비롯한 다양한 화학물질들이 생산된다. Drosophila melanogaster는 이러한 발효·부패 환경에 서식하면서 일정 농도 이상의 다양한 화학물질에 지속적으로 노출되어 생존하도록 적응되어온 것으로 생각된다. 다양한 화학물질이 포함한 환경에 안정적으로 서식하기 위해서는 D. melanogaster는 화학물질에 능동적으로 반응하여 해독 유전자나 대사 관련 유전자의 발현량을 변화 시킴으로써 발효·부패 환경에서 생성되는 화학물질에 대한 높은 내성을 가지고 있을 것으로 판단된다. 현재까지 유전자의 발현량 측정을 위해 real-time PCR를 이용하여 reference gene의 발현량을 기준으로 정량화하는 방법이 가장 널리 사용되고 있다. 그러나 조직별, 환경별, 발달단계를 비롯한 다양한 조건에서 안정적으로 발현되는 reference 유전자 선정이 필수적으로 선행되어야 하므로 본 연구에서는 발효·부패 환경에서 생산되는 두 화학물질인 Methanol과 Ethyl Acetate에 노출된 D. melanogaster에서 안정적으로 발현되는 reference gene을 찾는 연구를 실시하였다. 본 연구에서는 다양한 농도의 Methanol과 Ethyl Acetate을 D. melanogaster에 노출시킨 후 RNA 추출과 cDNA 합성을 실시였고, 5가지 후보 reference gene (hsp22, nd, rpL18, tbp and ef-1b)의 안정적 발현 여부를 qRT-PCR을 통해 조사하였으며, 유전자 발현의 안정성을 측정하는 3가지 프로그램(geNorm, NormFinder, BestKeeper)을 이용해 비교·분석하였다. 본 학회에서는 연구의 과정과 그 결과를 발표하고자 한다.

기후온난화 현상이 지속되고 있는 제주지역에서 환경적으로 다른 지역 모기의 계절적 발생밀도를 조사하기 위해 제주시의 국제공항, 항만 구역과 축사 그리고 서귀포 도심지의 11지점을 선정하여 3월부터 11월까지 매달 2회씩 Black light trap과 BG sentinel trap을 이용하여 모기를 채집하였다. 채집된 모기는 5속 7종. 6,042마리였으며, 이 중 빨간집모기(Culex pipiens)가 4,159마리(68.8%)로 우점종이었으며 흰줄숲모기 (Aedes albopictus)는 1,348마리(24.4%)였다. Black light trap를 이용한 채집에서 중앙동주민센터는 트랩당 72.8마리를 채집하여 모기 밀도가 가장 높게 나타났으며 제주국제공항은 트랩당 1.4마리로 가장 낮게 나타났다. BG sentinel trap을 이용한 채집에서는 항만에서 트랩당 71.7마리로 가장 많았고 도심지의 걸매생태공원에서 28.3마리로 가장 낮았다. 시기별로 모기의 밀도는 5월부터 증가하기 시작하여 8월에 1,156마리 (19.1%)로 가장 높은 밀도를 나타내었다. 채집된 암컷모기를 종별, 시기별, 지점별로 나누어 pool당 50마리 이하로 설정하여 총 364 pools에서 flavivirus 존재여부를 real time RT-PCR로 검사하였으나, 검출되지 않았다.

In this study, the odor generated in a livestock farm with 500 heads of finisher breed in 661 m² was monitored during 6 months using a gas sensor, a wired / wireless communication system and database server. Odor unit, ammonia, hydrogen sulfide, and total volatile organic compounds (TVOC) were monitored using the gas sensor. To show the tendency of odorous substances generation, the odor concentration was shown in the graph on a monthly and daily basis. Among the analysis items, the maximum generation of odor was found to be closely related to the generation of hydrogen sulfide. Through observing the daily and monthly trends of odor substances, it was found that each substance was a useful indicator for monitoring odor, because ammonia, hydrogen sulfide, odor and TVOC were increased and decreased in a similar pattern. The odors were highest in the hours of the early morning (00:00-05:00), the evening (18:00-23:00), and the morning (06:00-11:00) in a day. After the use of the microbial agent was discontinued in autumn (October), anaerobic digestion of the manure in a pit proceeded and the amount of hydrogen sulfide increased. Therefore, despite a slight decrease in ammonia production, the odor unit level did not decrease after October but rather was somewhat increased. In the future, the use of the odor monitoring system is expected to improve the efficiency of odor sources management.

This study suggests a model of production information system that can reduce manufacturing lead time and uniformize quality by using DNC S/W as a part of constructing production information management system in the industrial field of the existing marine engine block manufacturing companies. Under the effect of development of this system, the NC machine interface device can be installed in the control computer to obtain the quality information of the workpiece in real time so that the time to inspect the process quality and verify the product defect information can be reduced by more than 70%. In addition, the reliability of quality information has been improved and the external credibility has been improved. It took 30 minutes for operator to obtain, analyze and manage the quality information when the existing USB memory is used, but the communication between the NC controller computer and the NC controller in real time was completed to analyze the workpiece within 10 seconds.

본 연구는 국내에서 생산되거나 해외에서 수입되어 국내에서 유통되는 수산물 중에서 두족류를 문어류, 낙지류, 오징어류, 주꾸미류, 꼴뚜기류의 5개 그룹으로 구분하여 분석하였다. 두족류 5개 그룹을 판별을 하기 위해 미토콘드리아에 존재하는 유전자를 분석하였고, 그 중에서 COI (mitochondrial cytochrome C oxidase subunit I), 16s rRNA (16s ribosomal RNA), 12s rRNA (12s ribosomal RNA) 내에서 상당히 유사한 DNA 서열 부분과 일부 서열 변화 부분이 확인되었다. 명확하게 두족류 5개 그룹 판별을 하기 위해 COI, 16s rRNA, 12s rRNA 유전자의 일부 서열 변화 부분에서 그룹 특이적 프라이머 세트를 디자인하였다. 국내·외에서 확보한 두족류 시료(참문어, 낙지, 살오징어, 아메리카 대왕오징어, 갑오징어, 주꾸미, 모래주꾸미, 하이야주꾸미, 참꼴뚜기, 창꼴뚜기, 한치꼴뚜기)의 genomic DNA을 추출하여 각 그룹의 특이적 프라이머를 이용하여 SYBR 기반의 real-time PCR 시스템에 의해 분석되었고, threshold cycle (Ct) value와 같은 real-time PCR 결과 분석에 의해 두족류 내 그룹 판별이 가능하였다(Table 3).

Introduction Recently, the popularization of mobile devices has been changing the pattern of customers’ life. In the online market, the distribution market has been also expanding from PC-based online to mobile shopping using smart phones. As the shopping market using mobile has grown rapidly in the developing smart age, the interest of home shopping is moving toward mobile media. Through the home shopping company's web site and mobile application, live broadcasting, real-time communication service, and other customers’ review are performed at the same time. Home shopping customers who have questions or inquiries about products are meeting salespeople through home shopping mobile application and real-time communication service. The significance of this study is to present effective strategies for connecting live home shopping and real-time communication of mobile application, thereby enabling customers to make smart consumption. Through this service, home shopping company can perceive customers’ needs as well as form cocreation value with customers. In addition, home shopping company aims to provide a more useful and effective method in the process of providing real-time communication service by checking the form of customers participating in real-time communication service. Theoretical Development Although there are many prior researches on adaptive selling on salespeople in offline, research on effective sales strategies of live home shopping and real-time communication using application has not yet been conducted. Nowadays, many customers' purchasing methods are moving from offline to online. Therefore, by referring to similarities between virtual world and actual society in Internet space studied in many previous studies, we would like to find out whether the offline advantage of "adaptive selling tactics" in the sales field can be applied to on-line. Adaptive selling means changing sales behavior throughout the interaction or interaction with the customer based on perceived information about the sales situation. Salespeople need to change their sales behavior in order to meet each sales situation or individual customer's desires. Adaptive selling can be said to modify the communication style and content according to the customer's desire (Weitz, Sujan & Sujan, 1986; Spiro & Weitz, 1990). The main reason for the co-creation value activities of companies and customers is that the marketing paradigm is shifting from good-dominant logic to service-dominant logic (Lusch & Vargo, 2006). This means that new value creation areas of new product development are expanding as customers participate in various decision-making related to the companies (Payne, Storbacka & Frow, 2008). To realize the value proposition, a firm must co-create value with its customers by means of direct interaction (Grönroos & Voima, 2013). Research Design The contents of this study are as follows. First, we divide the adaptive selling strategies of salespeople in real life into live home shopping and mobile application roles, and then examine whether live home shopping and mobile application affect customer trust. In previous study, Frazier and Summers (1984) proposed six tactics – information exchange, recommendation, request, threats, promises, and legalistic pleas, and Richard et al. (2006) proposed another six tactics. Anderson and Weitz (1989) define trust as one party’s belief that its needs will be fulfilled in the future by actions undertaken by the other party. Second, we investigate the effect of customer trust on home shopping commitment relationship. Ganesan (1994) defined relationship commitment as the desire to develop stability of relationship, the belief that the relationship is sustainable, and the willingness to make short-term sacrifices to maintain the relationship. Through this process, we are able to confirm the possibility of replacing actual meeting with tool called online real - time communication. Third, we want to find out the effect of home shopping commitment relationship on brand commitment relationship. Even if the same brand product, we can know the effect of commitment relationship depending on home shopping company. Finally, we are going to find out how to create co-creation values with customers by real-time communication service to solve the questions and problems of customers immediately. The creation of co-creation values between customers and companies can increase the success rate of new product development that reflects the core needs of customers and reduce resources such as corporate marketing and R & D, which can ultimately serve as a driving force for creating sustainable competitive advantage (Vernette & Hamdi-kidar, 2013).

With the increasing prevalence of smartphones, marketers have a keen interest in the mobile channel hoping to enhance the effectiveness of marketing activities and improve sales performance. Prior research on mobile marketing examines for example, coupon redemption, time sensitivity, and message optimization, and find that marketing activities and the corresponding performance should be understood based on contexts in which such actions are employed. Following this stream of research, we focus on the context in which consumers interact with mobile phones, and evaluate the role of real-time mobile coupons in driving e-commerce interest. To this end, we collaborate with an online retailer selling beauty products as well as a credit card company distributing mobile coupons on behalf of the online retailer. Target customers are selected using prior usage of credit cards such as category preference and spending amount. When SMS texts are being sent to smartphones with transaction confirmation, mobile coupons are attached to such texts in order to induce target consumers to sign up for the online retailer. Thus our data comes from two sources. The credit card company provides transaction information along with demographics and the online retailer track consumers’ behavior in log and purchase data. Our empirical analyses suggest that real-time contexts matter in determining the effectiveness of mobile coupons. To be specific, consumers who have just spent a small amount of money show a greater propensity to redeem the coupons despite small rewards. Also, heavy users of e-commerce tend to be more responsive to the coupons promoting the online retailer whereas those with large spending on beauty products are less likely to sign up. We discuss theoretical contributions of these findings and offer practical insights.

소비자들의 구매 욕구를 충족시키기 위하여 상업시설에서는 판매와 관련된 다양한 공간표현이 요구되고, 소비자의 시선을 끄는 상품의 배치나 공간구성은 곧 마케팅 전략으로 이어질 수 있다. 사람의 눈은 가장 많은 정보를 획득하는 감각기관으로 시각정보에 대한 분석은 <공간과 사용자>의 상호간의 시각적 관련성을 파악할 수 있는데, 그간 연구에서는 실험이미지를 통해 정지된 화상에 대한 분석이 대다수 이며, 상업공간의 이동영상을 바탕으로 공간에 대한 시선정보를 분석한 연구는 미비하다. 이에 본 연구에서는 상업시설의 이동경로를 촬영한 동영상을 실험에 사용함으로써 공간분석에 있어 사실감이 부여된 공간 사용자 시선정보를 통한 감성반응을 분석하였다. 이동동선의 직선구간을 분석구간으로 설정하고, 획득된 데이터를 바탕으로 5개의 주시강도 범위에 대한 구간특성을 살펴본 결과, 이동동선의 시작점인 A구간(직선구간-1)에서는 주시강도가 낮게 나타났으며, B구간(직선구간-2)에서 가장 높은 주시강도가 나타난 것을 알 수 있다. 이는 피험자들의 B구간부터 안정적인 공간 탐색을 한 것으로 볼 수 있으며, 실험영상에 대한 적응시간이 필요한 것을 알 수 있다. 주시집중구역에 대한 공간특성을 살펴보면, 6개 구간 중 4개 구간에서 우측매장의 진열방식에 대한 시선의 집중이 더 높게 나타난 것을 알 수 있다. 소비자의 시선이 높게 머무른 요소는 소품위주로 시선이 유도되었으며, 매장 내 쇼케이스 같은 진열형식에 시선정보가 높게 나타난 것을 알 수 있다. 이러한 분석방법을 통해 소비자의 시선이 선호하는 상품진열방법, 상품요소 배치 등 직접적인 디자인 자료로 활용 가치가 높게 제공 될 수 있을 것으로 사료된다.

Structure behaviors resulting from an earthquake are experimentally simulated mainly through a shaking table test. As for large-scale structures, however, size effects over a miniature may make it difficult to assess actual behaviors properly. To address this problem, research on the hybrid simulation is being conducted actively. This method is to implement numerical analysis on framework members that affect the general behavior of the structure dominantly through an actual scale experiment and on the rest parts by applying the substructuring technique. However, existing studies on hybrid simulation focus mainly on Slow experimental methods, which are disadvantageous in that it is unable to assess behaviors close to the actual level if material properties change depending on the speed or the influence of inertial force is significant. The present study aims to establish a Real-time hybrid simulation system capable of excitation based on the actual time history and to verify its performance and applicability. The hybrid simulation system built up in this study utilizes the ATS Compensator system, CR integrator, etc. in order to make the target displacement the same with the measured displacement on the basis of MATLAB/Simulink. The target structure was a 2-span bridge and an RC pier to support it was produced as an experimental model in order for the shaking table test and Slow and Real-time hybrid simulations. Behaviors that result from the earthquake of El Centro were examined, and the results were analyzed comparatively. In comparison with the results of the shaking table test, the Real-time hybrid simulation produced more similar maximum displacement and vibration behaviors than the Slow hybrid simulation. Hence, it is thought that the Real-time hybrid simulation proposed in this study can be utilized usefully in seismic capacity assessment of structural systems such as RC pier that are highly non-linear and time-dependent.

Three CNN (Convolutional Neural Network) models of GoogLeNet, VGGNet, and Alexnet were evaluated to select the best deep learning based image analysis mothod that can detect pavement distresses of pothole, spalling, and punchout on expressway. Education data was obtained using pavement surface images of 11,056km length taken by Gopro camera equipped with an expressway patrol car. Also, deep learning framework of Caffe developed by Berkeley Vision and Learning Center was evaluated to use the three CNN models with other frameworks of Tensorflow developed by Google, and CNTK developed by Microsoft. After determing the optimal CNN model applicable for the distress detection, the analyzed images and corresponding GPS locations, distress sizes (greater than distress length of 150mm), required repair material quantities are trasmitted to local maintenance office using LTE wireless communication system through ICT center in Korea Expressway Corporation. It was found out that the GoogLeNet, AlexNet, and VGG-16 models coupled with the Caffe framework can detect pavement distresses by accuracy of 93%, 86%, and 72%, respectively. In addition to four distress image groups of cracking, spalling, pothole, and punchout, 22 different image groups of lane marking, grooving, patching area, joint, and so on were finally classified to improve the distress detection rate.

This study suggested a new real-time traffic signal operation algorithm using combined data of travel time and occupancy rate. This study applied the travel time data to traffic signal control system, and developed the signal operation algorithm based on saturation degree that was calculated using the travel time data. This algorithm calculates a queue length using a delay model, and converts the queue length to the saturation degree. Moreover, it calculates signal timing variables using this combined saturation degree. This study conducted a microscopic simulation for effectiveness evaluation. We checked that the average intersection delay decreased by up to 27 percent. Moreover, we checked that this signal operation algorithm could respond to a traffic condition of oversaturation and loop detector error effectively and usefully. In korea, sectional traffic detection systems are being installed in various ITS projects, such as Advanced Transportation Management System(ATMS) and Urban Transportation Information System(UTIS). This study has important significance in the sense that it is new methodology to accept the sectional detection system in traffic signal control system.

Honey bee has been widely used as a model insect for biological sciences because of its sociality and specialized labor division. For the investigation of the seasonal and labor-dependent expression patterns of genes putatively involved in its sociality, quantitative real-time PCR (qRT-PCR) can be applied to quantify gene expression level and selection of reliable reference gene(s) for normalization is an accurate step. In this study, using three softwares (geNorm, NormFinder and BestKeeper), we evaluated seasonal expression stabilities of four reference genes that have been widely used for qRT-PCR in forager and nurse heads. Among four candidates, two genes, rpS18 and gapdh, were suggested to be the optimal reference genes for qRT-PCR.

Salmonella는 전세계적으로 식중독을 유발하는 주요 원 인 균으로서, 식중독을 유발하는 Salmonella를 신속하게 검출하는 방법은 식품 안전을 위한 중요한 도구이다. Realtime PCR은 식중독균을 검출하기 위한 신속검사법으로 널 리 사용되어 왔다. 최근에는 NBS LabChip real-time PCR 이라는 새로운 시스템이 칩타입으로 조작이 간편하며 초 고속의 real-time PCR 시스템이라는 보고가 있었다. 본 연 구에서는 살모넬라의 신속한 검출을 위하여 NBS LabChip real-time PCR에 기반하여 real-time PCR 반응 시간이 20 분 이내인 검출법을 확인하고자 하였다. 프라이머와 프로 브 설계를 위해 두 개의 타겟 유전자(invA, stn)가 선택되 었으며, 특이도와 민감도(검출한계)를 평가함으로 개발된 검출법을 검증하고자 하였다. 본 연구에서는 특이도 검증을 위해 Salmonella 균주 42주와 Non-Salmonella 균주 21 주를 포함하였으며, 본 방법으로 Salmonella 42주에 대해 서만 정확하게 검출이 가능하였다. 검출한계는 살모넬라 genome DNA 기준으로 101 copies/μL 였으며, 소시지에서 는 4시간 증균 이후 접종균수로서 101CFU/g 에서 102 CFU/ g까지 검출이 가능하였다. 본 연구에서 개발된 검출법은 신속한 식중독 원인조사에 활용될 수 있을 것으로 기대된다.

Background: The continuous co-contraction of the trunk muscles through trunk stabilization exercises is important to patients with lumbar spinal stenosis (LSS). However, intentional abdominal muscle contraction (IAMC) for trunk stabilization has been used only for specific training in the treatment room. Objects: The purpose of this study was to provide feedback to adults with LSS to enable IAMC during activities of daily living (ADLs). Methods: The participants with spinal stenosis were divided into an experimental group of 15 adults and a control group of 16 adults. Electromyographic signals were measured while the subjects kept their both hands held up at 90°. The measured muscles were the rectus abdominis (RA), external oblique (EO), internal oblique (IO), and erector spinae (ES). Pelvic tilt was measured using a digital pelvic inclinometer. The degree of pain was measured using the visual analogue scale (VAS) and functional capacity was measured using the Korean version of the Oswestry disability index (KODI). Results: While the experimental group showed statistically significantly higher activities in the RA, EO, and IO after the intervention compared with the control group. Pelvic tilt was significantly decreased only in the experimental group. Both the experimental and control groups exhibited statistically significant declines in the VAS and KODI (p<.01). In terms of the levels of changes, the experimental group exhibited a statistically significant larger decline only in the VAS and the pelvic tilt when compared with the control group (p<.05). Conclusions: The subjects could stabilize their trunks, and relieve their pain and dysfunctions and reduce pelvic tilt by learning abdominal muscle contraction during ADLs.

작물생육의 품질 및 생산량에 중요한 영향을 미치는 온실 내 환경관리에 대한 연구는 활발히 진행되고 있다. 주로 온실 내 환경분포를 측정하는 방법으로는 한 두 지점에 대해서만 측정하여 온실 전체를 관리하는 시스템 으로 이루어졌으며 기존 환경데이터 측정방식은 각각의 데이터 로거 및 센서간의 배선들로 인하여 복잡한 시스템으로 구성되었다. 본 연구에서는 온실 내 설치 된 각 환경센서들로부터 지점별 데이터를 획득하고 획득된 데이터는 모니터링 프로그 램을 통하여 공기유동흐름을 측정하는 장치를 개발하였다. CAN 네트워크 통신을 통하여 환경센서들의 배선 토폴로 지를 간소화 했으며 프로토콜의 견고함으로 온실 내 모니 터링을 안정적으로 데이터를 수집할 수 있도록 구현되었다. 온실 내 공간의 환경요인 분포(온·습도 및 풍속 등) 들을 12개 지점에 배치하고 온·습도 및 풍속의 환경 데이 터는 상세히 파악할 수 있도록 X, Y, Z 축으로 다수의 측 정점(총 36점)을 선정하였다. 데이터 손실 및 다양한 온실 조건을 고려하여 비트레이트를 저속 125kbit/s로 구현하여 온실 내 100m 구역내에서 센서를 추가적으로 연장(총 90 개)할 수 있도록 구축되었다. 온도, 습도, 일사량, 풍향, 풍 속, 대기압 및 강우량 등 측정된 데이터는 LabVIEW에 연 동되어 실시간으로 센서 정보 출력이 가능하도록 구현되었다. 온실 내 환경 분포는 사용자의 편의에 따라 환경분포를 수평(XZ), 수직(YZ)축으로 가시화 할 수 있으며, 보간의 범위를 원하는 값으로 설정하여 보간 할 수 있도록 구현되었다. 추후에 온실 내의 공간에 따라 온도, 습도, 풍속, CO2 등의 환경 측정 실험을 통하여 CFD 모델링과의 검증 및 비교에 활용할 수 있을 것으로 판단된다.

Korean small and medium sized software companies have tried to export their solutions or services to overseas markets. In 2016, exports of the software industry increased by 6.0% from the previous year, and the value added of the industry was 2.2 times higher than that of the manufacturing industry. From a long-term perspective, it is important to secure a global competitive advantage in order to sustain the export high value-added of the software industry. The obstacles to entry into the overseas market of small to medium enterprises are as follows: first, difficulty in product development and localization of marketing; second, lack of investment for overseas expansion; and finally, competitiveness of software technology. In particular, To overcome such obstacles, Korean small and medium sized software companies should increase the technical perfection and secure software export competitiveness. The paper presents a payment settlement framework enabling adaptive reuse and semiautomatic development of global payment settlement services. The quantitative and qualitative evaluation results are presented with domestic and overseas case studies as follows: Firstly, semi-automatic development is realized successfully by applying the framework. Secondly, it is possible to maintain consistent quality of software and to deliver maintenance services without relying on the internal human resources. Thirdly, it is possible to reduce the project duration of the same development cope to less than 50% by applying the framework. Finally, because it is based on BPMN 2.0, which is a high level design diagram, it is expected that it will be easy to implement through components connection and reduce difficulties in technology transfer and localization. Also, at the time of runtime operation, it will be effective to understand the design idea easily and to carry out additional developments without human resource who participated in the initial project.