The average BOD concentration was found to be about 269mg/L before a process innovation, but after the innovation, it became 30mg/L, which satisfied the effluent standard of 120mg/L. The removal effluent standard of 120mg/L. The removal efficiency was about 60～80%, and the concentration of the treated water was found th be low. after the process innovation, the average COD concentration was 29mg/L, and the CODmn removal efficiency became low to the level of about 65～76%, which was found lower than the effluent standard of 130mg/L. After the process innovation the SS average concentration of the treated water was 13mg/L, which was lower than that before the innovation (32mg/L). By the activated sludge process innovation, the SS removal efficiency was improved to be 30～70%. The average concentration of total coliform before the process innovation was 6100 count/mL because an Activated sludge process only occasionally pass over the allowed standard(The average number of the total coliform of Activated sludge process treated water was about 8100 count/mL), UV disinfection process was introduced. after the introduction, the average number of the total coliform was 1800 count/mL, which satisfied the allowed effluent standard of 3000 count/mL.

한국형 잔디에 있어 병원성 (라지 팻치) 감염이 산화적 스트레스의 발현에 미치는 영향을 규명하기 위하여 라지 팻치에 감염된 잔디의 항산화 효소의 활성을 조사하였다. 처리 후 6일 동안 이틀간격으로 잎과 뿌리 시료에 대해 각각 분석하였다. 초기 2일 동안 SOD효소 활성은 처리 간 아무런 차이가 나타나지 않았으나 처리 후 6일차에는 대조구에 비해 라지 팻치에 감염된 잎에서 48% 뿌리에서 49% 각각 높게 나타났다. CAT 효소활성은 처리 후 2일간 잎에

한국형 잔디에 있어 병원성 (라지 팻치) 감염이 산화적 스트레스의 발현에 미치는 영향을 규명하기 위해 라지 팻치에 감염된 잔디의 생육, 뿌리 건사율, 과산화수소 농도 및 지질과산화 정도를 감염이되지 않은 대조구와 비교하였다. 처리 후 6일 동안 이틀간격으로 잎과 뿌리 시료에 대해 각각 분석하였다. 라지 팻치에 감염된 잔디 뿌리의 건사율은 대조구에 비해 약 30% 증가하였다. 건물 함량은 4일째까지는 유의적인 차이가 없었으며 6일차에서만 라지 팻치 감염에

병원균 감염에 의한 식물체내 프롤린과 암모니아의 농도 변화와 그것의 스트레스 생리학적 의미를 구명하기 위하여 Rhizoctonia spp.를 처리 후 라지 펫치에 감염된 잔디의 생육 및 관련 화학적 성분을 감염이 되지 않은 대조구와 비교하였다. 라지 펫치에 감염된 잔디의 뿌리의 건사율은 대조구에 비해 약 30% 증가하였다. 가용성 단백질 농도는 병원균 처리후 6일째 잎의 경우를 제외하고는 라지 펫치 감염에 따른 유의적인 영향이 없었다. 암모니아 농도 역

Cervical carcinoma is the 1st most common malignancy in korean females. HPV have been strongly linked to progression of cervical carcinoma. E6 and E7 as a small DNA virus encoding two major oncoproteins of HPV can act together to produce efficient immortalization of primary human epithelial cells, providing further evidence for the role of HPV in tumorogenesis. It is important to pursue the development of Immortalized human epithelial keratinocyte(IHEK) culture model which could be related to the pathogenesis between cervical and oral carcinoma. If we establish IHEK transfected by E6E7 gene, IHEK will be accepted as a model system for HPV-linked cervical carcinogenesis. The purpose of this study were to culture primarily normal human epithelial keratinocyte(NHEK), and to establish IHEK for applying these results to cervical and oral carcinogenesis in the future. The obtained results were as follows. 1. After 7-9 passages, cultured NHEK was almost senesce and disappeared, but cultured IHEK showed most basal cell and monolayer of polyhedral cells under 0.05mM Ca++, while small area of stratification and flattened epithelial cells with irregular border under 1.2mM Ca++. 2. The cultured IHEK showed relatively resistant growth to high calcium condition. 3. The mRNA E6E7 in cultured IHEK by RT-PCR was detected. 4. During the terminal defferentiation in cultured NHEK and IHEK, increase of insoluble cornified cell envelope formation was accompanied with induction of TGase 1 activity. But the cultured IHEK showed less CEM and TGase 1 activity than those of cultured NHEK. 5. Cultured IHEK showed non-tumorogenecity, but week anchorage independence. From the aboving results, we have developed technique to transform NHEK into IHEK by transfecting cells with E6E7 gene. Cultured IHEK was established as intermediate stage cell for studying the pathogenesis of human cervical carcinoma.

본 연구는 기호성이 높으며 여름철 고온에 강한 새로운 tall fescue 품종을 구명하기 위해 초지조성 후 5년째인 1997년 방목기간 동안에 북제주군 한림읍 이시돌 목장에서 홀스타인 육성우(방목개시시 체중 110kg 내외)를 이용하여 구당 250(50mx50m)의 면적에서 2처리(T1 = Fawn tall fescue + orchardgrass + perennial ryegrass + white clover, T2 = Roa tall fescue )