The members of the genus Flavivirus are noteworthy, as they cause infectious diseases in humans, such as Zika, denguefever, yellow fever, West Nile, and Japanese encephalitis. Due to the increased awareness of the public health risk posedby flavivirus-infected mosquitoes, mosquito collections were performed in six urban parks of South Korea, as the parksare designated for human recreation but also provide suitable habitats for mosquitoes. We examined the diversity andabundance of mosquito species and conducted molecular diagnostics for the detection of flavivirus infections. Monthlycollections were carried out in each park from March to August in 2017. A total of 4,851 mosquitoes (5 genera and13 species) were collected using BG-sentinel traps and then investigated for flavivirus infections. Pathogenic flavivirusinfections causing human diseases were not observed in the field-collected mosquitoes. However, insect-specific flavivirus(ISF) infections were detected in several mosquito pools. ISF has been previously known to enhance or suppress the replicationof medically important flaviviruses in co-infected mosquito cells. In this study, partial sequences of ISF were analyzed.However, further studies are needed in order to determine its genetic characterization and biological function in vivo.

The Pleurotus tuoliensis is a mushroom named Chinese ‘Bai-Ling-Gu’ from species of Pleurotus nebrodensis. we are mated for selection of cytoplasmic hybrid by mitochondria microsatellite marker and the method of Mon-Mon mating between monokaryotic strains derived from Pleurotus ferula ASI 0629 (Beesan No.1) and Pleurotus tuoliensis ASI 0663 (Baekryung 20). The cytoplasmic hybrids were identified 8 strains contained nuclear DNA bands of 'Baekryung 20' and mitochondrial DNA of monokaryon strains derived from Pleurotus ferula ASI 0629 (beesan No.1). The KiMB-Plft-15-81 was shown the best cultural characteristics, selected to be a new cultivar and designed as 'Baekwhang'. The 'Baekwhang' cab be grown without an extra after-ripening period, can utilize bottle cultivation of Pleurotus eryngii. And also the ‘Seolwon’ is similar to the existing Pleurotus tuoliensis in shape and physiological characteristics, formed high stipe. We therefore expect that this new strains will substitutional goods of Pleurotus eryngii.

The purpose of this study was to investigate the effects of lumbar stabilization and sling exercise on visual analogue scale (VAS), Korean Oswestry Disability Index (KODI), and stability index (ST) in patients with chronic lower back pain (CLBP). This study included participants with a VAS scale of 6 or higher and a KOD of 20-40%. They were randomly divided into a mat group (n=15) to perform lumbar stabilization exercises and a sling group (n=15) to perform sling exercises, and then they underwent a four-week experiment. The experimental results of this study were as follows: the VAS and KODI showed a statistically significant difference (P<.05) and a larger effect size within each group after the intervention, it exhibited no statistically significant difference between the two groups (P>.05). There were significant differences in ST before and after intervention in each group (P<.05), however there was no significant difference between the groups. The present study suggest that the effects of lumbar stabilization exercise and sling exercise are similar.

In a previous study, Peptoniphilus mikwangii was isolated from the human oral cavity as a new species. The purpose of this study was to develop P. mikwangii-specific PCR primers. The PCR primers were designed, based on the nucleotide sequence of 16S ribosomal RNA (16S rDNA). The specificity of the primers was tested using genomic DNAs of 3 strains of P. mikwangii and 27 strains (27 species) of non-P. mikwangii bacteria. The sensitivity of primers sensitivity was determined using PCR, with serial dilutions of the purified genomic DNAs (4 ng to 4 fg) of P. mikwangii KCOM 1628T. The data showed that P. mikwangii-specific qPCR primers (B134-F11/B134-R1 & B134-F5/B134-R5) could detect only P. mikwangii strains, and 400 fg or 40 fg of P. mikwangii genome DNA. These results suggest that PCR primers are useful in detecting P. mikwangii from the oral cavity.

Cherry leaf roll virus(CLRV)는 group IV positive sense ssRNA viruses, Nepovirus로 분류되는 식물병원성 바이러스이다. CLRV는 체리 등 목본 및 완두 등 콩과 작물을 자연 기주로 하며, 실험적으로 약 36개 과 이상의 넓은 기주 범위를 가지고 있어 국가적, 경제적 및 농가 개인적 피해를 야기 할 가능 성이 보고되고 있다. 현재 CLRV를 검출하는 방법으로 역전사(reverse transcription; RT)-nesdted 중 합효소연쇄반응(polymerase chain reaction; PCR) 이 활용되고 있으며, 다양한 기주로부터 CLRV를 검출하기 위해서는 검출 감도, 특이성, 반응 시간, 단순성 등이 중요 요소였다. 그러나 RT-nested PCR은 두 단계로 구성되어 있어 단순하지 않고, CLRV를 검출하는데 약 10시간 이상의 반응 시간이 소 요되었다. 이번 연구에서는 등온증폭법을 이용하여 단순하고 신속하게 CLRV를 검출하는 방법을 개발하 였다. 등온증폭 반응은 RT-nested PCR과 동등한 검출 감도로 CLRV를 검출 하였다. 그러나 반응 시간 을 약 2시간 수준으로 단축하였으며, 6개 영역을 사용하는 등온증폭 프라이머의 사용으로 더욱 특이적 으로 증폭 할 수 있었다.

Pine wilt disease is currently the most deadly forest disease in the world and is known to be caused by infection of Bursaphelenchus xylophilus. Until now, no method has been developed to confirm the pine tres infected with pine wood nematodes. In order to develop a method to diagnose pine wood nematode infection, we produced a monoclonal antibody against Expansin B3, which was found to be secreted by pine wood nematodes. ELISA assay using various monoclonal antibody confirmed that the pine trees infected with the rewarming can be detected. Further studies using our antibodies may help to develop a diagnostic method that can quickly confirm infection of pine wood nematodes in the field.

To investigate unknown molecualr and cellular mechanisms underlying polyphenisms in the migratory locusts, we performed whole transcriptome analysis from 12 different groups. Significantly differentially expressed coding and long non-coding (LNC) RNAs were present according to habitats, phases, and genders. Those differentially expressed coding and LNC RNAs were confirmed by quantitative-realtime-reverse transcriptase PCR. Those identified coding and LNC RNAs could be a target for developing environmental friendly management tools in the near future.

Rice stripe virus (RSV) is one of the serious plant pathogenic viruses for rice transmitted by small brown planthopper (SBPH), Laodalphax striatellus. RNA interference (RNAi) was recently suggested as a promising strategy for controlling insect pests, including those that serve as important vectors for plant pathogens. To disturb transmission of the RSV in SBPH using RNAi pathway, we chose nine genes highly expressed in RSV-viruliferous SBPH by transcriptome sequencing. These SBPH-derived dsRNAs were applied to the insects indirectly through xylem of rice leaves by irrigation. qPCR result demonstrated that five out of nine SBPH-derived dsRNAs successfully reduced the replication of RSV in viruliferous SBPH in dose-dependent manner, suggesting that these five dsRNAs could suppress replication of RSV and provide a new tool for RSV control strategy.

Insect infestation leads to huge loss of agricultural products and transmission of vector-borne diseases causing millions of deaths per annum. Juvenile hormone (JH) controls the development and reproduction of insects, therefore the grouth of insects can be inhibited by interfering the action of JH. Based on this, we developed a novel insect species-specific screening system to identify juvenile hormone antagonists (JHANs) from natural origin. These compounds can disrupt JH-mediated insect development by interfering the binding of a heterodimer, steroid receptor coactivator (SRC), with its partner protein, the methoprene-tolerant (Met) JH receptor. This screening system could be used as a new tool to develop eco-friendly and species-specific insecticides.

PURPOSES :With the increasing number of older drivers in an aging society, there is a growing need for research and planning on traffic safety for the older drivers using an improved road geometry design. This study also proposed a modified urban road interchange design, which aims to keep the older drivers away from accident-prone and high-traffic areas of the city.METHODS:In this study, we examined accident data records of older drivers to identify accident-prone zones and intersections; we studied the road geometry at these zones and analyzed if it was an underlying cause for higher number of accidents. Based on the research and subsequent analysis, we suggested plans for improvement of road geometry design at these intersections.RESULTS:By studying historic data and analyzing factors that affect the likelihood of accidents of vehicles driven by older drivers and after studying suitable traffic accident prediction models, we identified the major variables that need to be modified at accident-prone intersections, such as the width of a left turn lane at an intersection and the radius of the right turn lane at a street corner. The results have a significance probability of less than 0.001 and a 95% confidence level. To improve safety at the identified intersection, this study suggests the installation of a left-turn-lane-shaped Positive Offset and a right-turn-lane-shaped Slip Lane concept and an adjustment of intervals between intersections.

The aminoacyl-tRNA synthetases (ARSs) are ancient house-keeping enzymes that catalyze the ligation of tRNAs to their cognate amino acids in the first step of protein synthesis. During the evolution of higher eukaryotes, cytoplasmic ARSs have undergone significant changes including the addition of new domains that are not part of the enzymatic core. These additional regions have been found to be associated with a broad range of biological functions beyond protein synthesis. The non-translational functions of ARSs appear to be regulated by their presence within a cytoplasmic multi-tRNA synthetase complex (MSC), which is assembled through the appended domains. We recently reported that the MSC member glutamylprolyl- tRNA synthetase (EPRS) promotes antiviral gene expression through its infection-specific phosphorylation and release from the MSC. Here, we conducted transcriptome analysis of influenza A virusinfected cells. We particularly focused on the analysis of chemokine-related gene expression, in combination with chemokine array analysis against virus infection. Moreover, the correlation between chemokine expression pattern and EPRS function in response to different stimuli was assessed. The results showed that viral infection increases interferon-response and pro-inflammatory chemokine expression. In contrast, the level of chemokine expression was suppressed in interferon-γ treated cells. Thus, these results further demonstrate the previously reported stimulus-specific EPRS functions in immune responses.

Neuron-specific enolase (NSE), a brain specific isoenzyme of the glycolytic enzyme, is characterized by its consistent occurrence in the cytoplasm of mature neurons. The purpose of the present study was to investigate the expression of NSE in the developing tongue of Korean native goats. The tongues of the fetuses were removed from 2- to 4-year-old female Korean native goats by caesarean section performed under general anesthesia. The expression of NSE in the developing tongue of goat fetuses (60, 90, 120, and 150 days) was studied using immunohistochemistry. In 60-day-old fetuses, NSE-immunoreactivity (IR) exhibited weak appearance in lamina propria of the basal portion and the apical epithelia of the tongue. In 90-day-old fetuses, NSE-nerve fibers were extended in the core part of connective tissue, and primordia of the taste buds was moderately positive. In 120-day-old fetuses, NSE-IR was strongly expressed in taste buds and gustatory nerve fibers. In neonates, the taste buds of vallate papillae were strongly positive for NSE, and development of nerve fibers was synapsed with connective tissue of well innervated taste buds. These results indicate that NSE expressions were associated with the sign of nerve innervation in prenatal development of goat tongues and NSE may be a useful neuronal marker to understand the development of gustatory nerve innervations.

This research is about visualizing data for efficient use in agriculture, by measuring the understanding of graphs according to their features. A questionnaire consisting of one graph, either a bar graph or line graph, and a question about data, was made. According to the results, the time spent answering the question did not differ between the line graph and the bar graph. However, the rate of correct answers differed: that of the bar graph was 50% and the line graph was 0%. This implies that participants answered using the bar graph more accurately regarding whether the data involved understanding the absolute value. From this result, it is clear that using proper features of graphs according to the characteristics of data improves the understanding of information. In addition, a strategy to utilize information and communication technology in agriculture effectively was discussed.

본 연구는 평택항을 통항하는 선박의 항해 안전을 확보하기 위해 교통안전특정해역 설정의 필요성을 정량적인 데이터로 제시하고자 하였다. 연구 비교 대상 6개 항만과 평택항과의 안개 일수, 도선 거리, 대형선 입항 척수, 조업 어선 척수를 비교 분석하였다. 연구결과 평택항은 잦은 시계 제한의 영향을 받는 역 조건의 가운데 부산, 부산신항, 울산, 포항항보다 약 3.5 ~ 6배 더 긴 도선 구간을 항해 해야한다. 특히 어장이 형성되는 5 ~ 7월에는 통항로에 위치한 35척의 조업 어선을 피하기 위해 대형선이 역주행하는 상황이 발생하여 상대선박과의 충돌 위험을 갖는 교통 환경임을 확인하였다. 따라서 평택항 접근수역에 교통안전특정해역으로 설정한다면, 대형선이 조업 어선 보다 법적 우위를 갖게 되므로 통항 안전성을 확보할 수 있을 것으로 판단된다.

Early pregnancy results in th production of various signal molecules such as steroids, prostaglandins, and many protein factors. The proteins especially produced by the placenta have been used to detect pregnancy for many years in other species. More recently, pregnancy-specific protein B, which is a placental glycoprotein can be measured by RIA or proteomic methods in serum of pregnant cow. And 2D Fluorescence difference gel electrophoresis (DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein expression analysis between samples. For this reason, we are analyzed serum of bovine. The purpose of this study was to apply DIGE technique for identification of bovine pregnancy-specific proteins using bovine pregnant and non-pregnant serum samples. Serums of 2 pregnant Holstein dairy cattle at day 21 after AI and those of 2 non-pregnant were used in this study. The molecular weight and charge matched cyanine dyes enable pre-electrophoretic labeling of non-pregnancy and pregnant serum proteins which are then mixed and labeled with Cy2 were used as an internal standard. Two pools of proteins are labeled with Cy3 and Cy5 fluorescent dyes, respectively. Labeled proteins with Cy2, Cy3 and Cy5 mixed together and separated in same gel and then were detected by fluorescence image analyzer. The 2D DIGE analysis using fluorescence CyDye flour showed higher sensitivity and better reproducible results than conventional 2D gel electrophoresis. Approximately 1,500 protein spots were detected by 2D DIGE. The differentially expressed proteins were identified by MALDI-TOF Mass spectrometer. Total 16 protein spots differentially expressed in the pregnant serum were detected, among which 7 spots were up-regulated proteins identified as conglutinin precursor, modified bovine fibrinogen, IgG1 etc, and 6 spots were down-regulated proteins identified as hemoglobin, complement component 3, bovine fibrinogen, IgG2a etc. These results indicated that DIGE system could be advantageous for the analysis of serum proteomics diversified by physiological conditions.

Loop-mediated isothermal amplification (LAMP) is a rapid, specific, cost-effective detection method by amplifying nucleic acid under isothermal conditions. In this study, we used LAMP for detection of Hamiltonella defensa that lives as a facultive endosymbiont of whitefly ‘Bemisia tabaci’. We designed the Hamiltonella-specific primers by targeting 16S ribosomal RNA gene and validated the specificity of one primer set. To find the optimum temperature for our primer set, the LAMP reaction was held at the temperature, 60℃, 62℃ and 65℃. As a result, 62℃ was the optimum reation temperature for LAMP reaction. Specificity of primer set was tested by the reaction to both Trialeurodes vaporariorum and B. tabaci. After the whole procedure, the amplicons by LAMP were visualized by adding SYBR Green to the reaction tube.