The natural environment of Gulup-do should be preserved due to its unique ecological characteristics. A recent report stated that CJ group, one of Korea’s major business conglomerates (chaebols), plans to construct golf courses and resort facilities in Gulup-do. Such projects will destroy the delicate animal habitats of the island. The harmful chemical runoff from golf courses will also significantly damage the ecosystem. The purpose of this paper is to show that the development of Gulup-do will adversely affect the ecology of the island.

본 문에서는 중국의 1956년 최초로 자연보호구 설치이후 자연보호구사업의 변화과정, IUCN과 중국의 자연보호구 정의를 비교한 토대 위에서 중국의 자연보호구 지정 목적과 의의를 설명하고 자연보호구의 현황을 소 하였다. 또한 중국의 50여년간의 자연보존과 자연보호구사업 변화과정에서 자연보호구사업과 연관성이 있는 법률과 규정의 제정 과정을 분석하고 자연보존과 자연보호구사업에서 이러한 여러가지 법률과 법규의 역할을 검토하였 며 이를 토대로 중국에서 자연보존 및 자연보호구사업 추진과정에서 점차 형성된 전반적인 자연보호구 지정과 설치 련 절차 및 자연보호구의 3개 종류, 9가지 유형에 대한 분류체계를 상세하게 설명하고 중국 실정에 부합되는 자 보호구 관리체제 와 특성에 대해서도 소개하였다. 마지막으로 중국의 자연보호구 중장기사업계획을 소개하고 현재 중 의 자연보호구 관리사업과정에서 존재하는 문제점과 향후 해결하고자 하는 문제점에 대해서도 검토하고 해결방향을 제시하였다.

콘택트렌즈 관리용액의 보존제 성분 중 Benzalkonium Chloride<BAC) 가 L929세포 주에 세포독성과 세포 자연사룰 유도하는지 조사하고자 시행하였다. 생쥐 섬유모세포 인 L929 cell line을 배양하여 양성 대조군으로 0.01% H20~， 음성 대조군으로 Media 를 처리하였으며 세포독성은 BAC룰 배양세포에 시간별 (24， 때hr) ， 농도별(0.0001 ， 0.001, 0.005, 0.01, 0.05, 0.1%) 로 처리하여 대조군과 처리꾼올 비교하여 세포독성 농 도를 산출하였다. Hematoxylin-Eosin Staining후 광학현미 경으로 형태적 변화를 관 찰하고， MTT Assay와 LDH Activity 로 검정하였으며， MTT 정량과 LDH(Lactate Dehydrogenase) 활성도로 세포독성 농도룹 산출하였다. MTT 정량결과， 세포 독성이 0.0001% 에서는 8%, 0.005% 에서는 30%, 0.001%에서논 65%5'_ 농도 의존적으로 독성 이 나따났으며， 0.001% 에서 LDH 유출정도가 대조꾼과 비교하여 차이를 나타내지 않 았으나， 0.01% 의 농도에서 2.9 배， 0.1%의 농도에서 대조군에 비해 3.35배정도 높은 LDH 의 유출율을 보였다. 세포자연사 (Apoptosis) 륜 관찰하기 위 해 Hoechst33342와 DNA Laddering과 Flow cytometry를 실시한 결과， 0.1%에서쓴 세포괴사 (necrosis) 에 의해， 0.001%-0.01%에서는 세포자연사(apoptosis) 에 의한다는 것을 알 수 있었다.

주왕산 국립공원의 자연보존지구를 중심으로 분포하고 있는 천연림의 생육현황과 구조를 정확히 파악하고자, 이 지역에 21개의 방형구(20×20m)를 설치하여 식생을 조사하였다. Cluster 분석한 결과 두 개의 집단으로 분류되었다. 수종간의 상관관계는 서어나무와 물푸레나무, 졸참나무와 굴참나무, 생강나무와 국수나무 등의 수종들 간에는 비교적 높은 정의 상관관계를, 고로쇠나무와 쇠물푸레, 당단풍과 굴참나무 둥의 수종들 간에는 높은 부의 상관관계를 보였다. 본 조사지의 종다양도는 1.1306~l.2688로 비교적 높게 나타났다.

덕유산 국립공원의 고산지대 (해발 1,350m이상)를 대상으로 삼림식생의 실태와 삼림군집 구조를 정확히 파악하고자, 자연보존지구에 29개의 방형구(20×20m)를 설치하여 식생을 조사하였다. Cluster 분석한 결과 네 개의 집단으로 분류되었다. 수종간의 상관관계는 신갈나무와 들메나무, 들메나무와 노린재나무 및 싸리, 시닥나무와 주목, 함박꽃나무와 작살나무, 주목과 구상나무 등의 수종들 간에는 비교적 높은 정의 상관관계를, 신갈나무와 시닥나무, 주목 및 괴불나무 등의 수종들 간에는 높은 부의 상관관계를 보였다. 본 조사지의 종다양도는 0.9969~l.2217로 비교적 높게 나타났다.

This study aimed to develop strategies to re-establish the Park Nature Conservation Area in Bukhansan National Park, reflecting landscape ecological value by using the zonation program Marxan with Zones. Planning unit was set by watershed, and the basic data were mapped, considering topographical and ecological values. Mapped indicators were analyzed with the application framework of Marxan with Zones by indexing some indicators. The zones divided into Park Nature Conservation Area (Zone A), Park Nature Environment AreaⅠ(Zone B) which is reflected on the concept of Potential Park Nature Conservation Area and Park Nature Environment AreaⅡ(Zone C). The best solution for each of the scenarios was fixed through the sensitiveness analysis. From these, the final solution was selected considering five criteria including area ratio of conservation area and grouping. Lastly, the final solution was verified in the overlapped analysis with recent zonation. According to the results, the number of watersheds was 77, with an average area of 1,007,481 m2. In terms of basic mapping and indexation, the slope index and number of landscape resources for topographical property were average 0.22 and 38 places, respectively. Biotope index was average 0.69 and legally protected species was 14 species, reflecting ecological values. As the social and economic indicators, trail index was average 0.04, and the number of tour and management facilities was 43 places. Through the framework of Marxan with Zones, the best solution for scenario 1 which was set by the highest conservation criteria was selected as the final solution, and the area ratio of Park Nature Conservation Area and grouping was excellent. As the result of overlapped analysis, suggested zonation of the Park Nature Conservation was better than the recent zonation in the area raito (28.3%), biotope gradeⅠ(15.4%) and the distribution points (10 places) of legally protected species with verification of proper distribution of conservation features according to the zone.

Juniperus chinensis (Cupressaceae) is a valuable woody plant resource, but this rare plant is only distributed in restricted regions in Korea. In this study, we surveyed the geographic distribution regions, variation of sex ratio and the dynamics of natural populations of J. chinensis. in order to establish conservation strategies for the sustainable conservation of this important resource tree. The 17 natural populations of J. chinensis are restrictively distributed in the shore cliffs of (1) Ulleungdo island, (2) Gyeongju-si to Gangneung-si of the eastern coast, the riverside cliff along (3) Donggang-river of Jungsun-gun and Pyeongchang-gun and (4) branch of Nakdonggang-river in Uiseong-gun. The populations in Ulleungdo island showed continuous population distribution of over 200 individuals, but populations in other regions had discontinuous population distribution of under 200. The population of J. chinensis surveyed in Korea was estimated in ca. 3,200 individuals. The sex ratio (male/female) among populations of J. chinensis was diverse with variation from 0.46~1.67 range, but showed no significance. The sex ratio of the total individuals by species level was 0.74, and the percentage of female tree was significantly higher than the male. The results of dynamics analysis within population among regions showed that seedlings and saplings of J. chinensis in three regions surveyed were continuously regenerating. The distribution of DBH class showed a stable population structure of inverse J shape graph, but fragmentation and decrease in populations because of invading broad-leaved shrubs and trees from the surroundings were concerned. Further studies, and in situ and ex situ conservation strategies for the sustainable conservation on natural populations of this rare resources woody plant, J. chinensis in Korea are recommended.

In Korea, a regulation of visual impact evaluation on development plan in natural environment is provided at the Natural Environment Conservation Act, but it was difficult to obtained the effectiveness enough to conserve natural landscape. So, the visual impact review on development plan is introduced to the act, through the revision of the Natural Environment Conservation Act in 2005. The basic directions of visual impact review are preservation, restoration, view protection, and harmony. The items of review are as follows; (1) development alternations for the provided plan, (2) executive reduction plan of visual impacts including consideration of growth of plants, (3) deliberation process between persons or parties concerned, etc. For rapid settlement of visual impact review system, it needs that the validity, the reliability, and the objectivity should be confirmed through steady research about reasonable guidance of review.