This study was carried out to investigate efficacy of aerosol sanitizer with natural antimicrobial and organic acids against Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes. The artificially inoculated pathogens on stainless steel coupon were treated with grapefruit seed extract (GFE), acetic acid,citric acid and lactic acid in model cabinet for 5 min. The number of three foodborne pathogens with individual treatment was reduced by 0.34-3.77 log units, treatment with GEF + organic acid was reduced by 1.72-3.89 log units and treatment with GEF + organic acid + alcohol was reduced by 1.46-5.05 log units. By treatment with GEF + lactic acid + alcohol in scale-up model system for 10 min. Populations of E. coli O157:H7, S. Typhimurium and L. monocytogenes were reduced by 3.42, 2.72 and 2.30 log units from the untreated control respectively. From the above result,aerosol sanitizer with natural antimicrobial agents and organic acid can be used as an environmental sanitation method with satisfying the consumer demand on safe food.

Antifungal activity of seven natural antimicrobials, such as ε-polylysine, extract of Yucca shidigera, TLS (vitamin B1 derivative), BMB-FS, chitooligosaccharide, KDSP 001, and KDSP 002 were investigated for their applications in functional beverage. Five fungi including Aspergillus niger, Penicillium citrinum, Rhizopus oryzae, Fusarium moniliforme, and Mucor rouxii were applied as test fungi and mininum inhibitory concentrations (MICs) of antimicrobials were examined. TLS exhibited the strongst antifungal activity among tested antimicrobials and the growth of all fungi was inibited at 100 ppm. The antifungal activity of BMB-FS appeared different. The growth of Fusarium moniliforme was inhibited by BMB-FS at 100 ppm, but it exhibit antifungal activity on P. citrinum, and M. rouxii at 1000ppm. MICs of TLS appeared to be 60 ppm for four test fungi except A. niger (100 ppm). These results indicate the possible usage of TLS and BMB-FS as natural antimicrobials in functional beverage.

The purpose of this study was to investigate the antimicrobial activities of natural antimicrobials (10 formulas, F1~F10) against yeasts in functional beverages. The growth rates of yeasts were different with the ten different natural antimicrobial formulas tested. Yeasts grew for 14 days and the antimicrobial effect was observed between 14 and 18 days. Levels of S. cerevisiae, Z. bailii, and P. membranaefaciens were reduced to the limit of detection (ND) < 10 CFU/mL) after 28 days. Resistance against the antimicrobial effect was greatest for P. membranaefaciens, which grew to a level of 0.12~1.48 log10 CFU/mL after 14 days and was reduced to a level of 1.61~3.55 log10 CFU/mL after 28 days. The resistance of C. albicans was also high with a growth level of 0.13~1.28 log10 CFU/mL after 14 days and reduction to 1.51~5.30 log10 CFU/mL after 28 days. The antimicrobial effect of F10 was strongest for P. membranaefaciens. Every treatment reduced the microbial levels to 2.68~5.62 log10 CFU/mL after 6 months. F2, F4, F5, F6, and F10 reduced the C. albicans level to ND after 28 days while F1, F3, F8, and F9 reduced yeasts to the ND level after 6 months. The antimicrobial activities observed here will be useful for development of natural antimicrobials.

본 실험에서는 가공 두부를 천연항균소재인 자몽종자추출 물의 유도체에 침지 처리한 후 꺼내어 상온에서 저장하면서 가공 두부의 품질(총균수, 수분 함량, 조지방의 함량, 조단백의 함량, 표면색도)변화에 미치는 영향을 무처리 대조구와 비교하면서 조사하였다. 천연항균소재의 희석액에 침지처리한 가공두부는 대조구에 비하여 수분함량, 조단백 및 조지방 함량이 높게 나타났으며, 총균수는 천연항균소재 처리구의 증가폭이 무처리 대조구에 비해 감소하는 경향으로 나타

항균력의 우수성이 확인된 천연항균소재인 대황추출물, 황련추출물 및 자몽종자추출물을 시설채소산물인 호박의 선도유지용 표면처리제 및 포장소재로 개발하여 저장실험을 실시한 견과는 다음과 같다. 호박을 항균소재에 침지처리한 후, 항균필름에 포장하고 1에서 저장하면서 저장호박의 경도, ascortbic acid함량, 중량손실을, 미생물 성장도 및 부패율을 측정한 결과, 대조구에 비교해서 긍정적인 결과를 얻을 수 있었다. 이는 대조구(LDPE) 필름에 비교

풋고추를 수확하여 선도를 유지할 수 있도록 하기 위하여 천연항균소재를 이용한 항균용제와 항균포장필름에 대한 각각의 효과와 두 가지를 병행한 상승효과를 확인하기 위하여 풋고추를 항균용제에 침지 처리후 1에 저장하면서 품질변화를 측정하였다. 그 결과 항균용제와 항균포장필름에 대한 각각의 효과뿐만 아니라 두 가지를 병행하여 저장하는 경우 상승효과가 더욱 두드러지게 나타나는 것을 확인 할 수 있었다. 따라서 풋고추의 선도를 유지하는 데에 천연항균소재가

To investigate the effect of several bactericides and fungicides against fruit & vegetable decay grapefruit seed extract(GFSE) was tested in a dilution solution. GFSE was shown to be effective against decay and rind breakdown and to extend the shelf-life of fruits and vegetables during storage and tiansport. Antimicrobial efficiency of GFSE on a wide spectra of gram + & - bacteria, moulds and yeasts was demonstrated by the measurement of minimal inhibitory concentrations performed (Bacteria 10-500ppm, Fungi:250-1,000ppm, Yeasts:100-250ppm). GFSE was separated and extracted into water-soluble fraction, water-insoluble and non-dialyzed fraction to isolate the antimicrobial substances. The water-soluble fraction showed the most active antimicrobial effect. The antimicrobial substances were isolated by gas chromatography. As the result of the isolation using GC, Peak-D was found to be the antimicrobial compound in GFSE. The identification of the most antimicrobial substance was carried out by using GC-MS.