Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 g/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.

Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 μg/mL and LOQ was 15.12 μg/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.

목적 : 세 가지 컴퓨터 마우스 작업 시 보조기 유형(미착용, 손목보조기, 팔꿉보조기)에 따른 근활성도 차이를 비교하는 것이다. 연구방법 : 부산광역시에서 근무하는 20∼40대 사무직 근로자 36명을 대상으로 보조기 유형(미착용, 손목보조기, 팔꿉보 조기)에 따라 세 가지 컴퓨터 마우스 작업에 대한 근활성도를 비교하였다. 세 가지 마우스 작업은 과제 1(8개의 칸에 마우 스 왼쪽 버튼을 이용하여 지그재그 클릭하기), 과제 2(검지를 이용하여 6페이지 분량의 문서를 마우스 휠을 돌려서 가장 마지막 페이지로 이동하였다가 다시 되돌아 오기), 과제 3(모니터 바탕화면에 깔린 아이콘 별로 폴더에 드래그하여 옮기기 로 구성하였다. 과제 수행 중 대상자의 근활성도는 표면 근전도 장비를 사용하여 등세모근, 손가락굽힘근, 긴노쪽손목폄 근, 자쪽손목폄근을 측정하였고, 결과분석은 반복측정 분산분석을 사용하였다. 결과 : 과제 1에서 손목보조기 착용 시 긴노쪽손목폄근의 근활성도가 유의하게 감소되었고, 팔꿉보조기 착용 시에는 긴노 쪽손목폄근 및 자쪽손목폄근의 근활성도가 유의하게 감소되었다. 과제 2에서는 손목보조기 착용 시 및 팔꿉보조기 착용 시 모두 긴노쪽손목폄근과 자쪽손목폄근의 근활성도가 유의하게 감소되었다. 과제 3에서는 미착용, 손목보조기 착용, 팔 꿉보조기 착용 간 통계학적으로 유의한 근활성도 차이가 없었다. 결론 : 과제 유형에 따라 손목보조기 또는 팔꿉보조기의 착용 효과가 근육별로 다르게 나타났으며, 일부 과제에서 보조기 착용이 긴노쪽손목폄근 또는 자쪽손목폄근의 근활성도 감소에 효과가 있는 것으로 사료된다.