The effect of the surrounding vegetation on the seed germination and growth of mountain-cultivated ginseng (MCG, Panax ginseng C.A. Meyer) was investigated. Seed germination rate and growth were tested for allelopathy effects on four forest tree species after treatment with fallen leaves and leaf extracts. In the case of soil treatment through fallen leaves and crushed leaves, the germination rate was lower in the Quercus myrsinifolia treatments, and the average germination time was slower when Chamaecyparis obtusa was treated. In the case of Pinus densiflora and Quercus variabilis, which are used in most of the MCG cultivation areas, they did not have a significant effect on seed germination. In the fallen leaves treatments, the stem showed a tendency to lengthen. The hot water extract treatment showed a higher germination percentage than the cold water extract treatment. The extract treatment showed a deficient germination percentage of some MCG seeds. However, in the case of the treatments except for this, the germination percentage was similar to that of the control treatment. However, the Mean Germination Time, germination rate, and germination value were faster and higher than the control treatment. As a result of calculating the allelopathic index (AI) of MCG according to the extract treatment of 4 species, most had a negative effect on germination, and P. densiflora and Q. variabilis extracts showed the most significant effect. The ginsenoside content was higher in the fallen leaves treatment than in the control. The above results will help select and manage MCG plantations.

본 연구는 allelopathic potential을 지닌 벼의 교잡종을 포장에서 재배하여 그 식물체의 잎을 생물검정에 이용하여 allelopathy 효과 유무를 검정하고, 생물검정으로 선발된 교잡종을 대상으로 phenolic compounds를 조사하여 allelochemical로써의 가능성을 확인하고 나아가 phenolic compounds 생합성에 관여하는 효소의 활성 측정과 이 효소의 작용에 의해 유도되는 phenolic compounds를 구명한 결과는 다음과 같다. 1. 포장상태에서 피를 대상으로 생물검정을 실시한 결과 양친으로는 Kouketsumochi, Woo co chin yu에서 공통적으로 90% 이상의 높은 피뿌리 생장 억제율을 보였고, 교잡종에서는 Kouketsumochi/Woo co chin yu, Dongjinbyeo/Kouketsumochi, Dongjinbyeo/Woo co chin yu 조합에서 80% 이상의 높은 억제율을 나타냈다. 2. Kouketsumochi의 PAL 활성은 protein 1kg당 로 타 공시품종보다 높았다. 또한 Kouketsumochi와의 교잡종도 타교잡종보다 높은 것으로 나타났다. 3. Kouketsumochi의 cinnamate 함량은 생체중 1g당 으로서 다른품종이나 교잡종보다 2~5배 높았고, Kouketsumochi와의 교잡종도 타교잡종보다 높았다.

This study was conducted to evaluate the allelopathic effect of aqueous extract of Ganghwa domestic mugwort (Artemisia spp.) on vegetables and its related allelochemicals. When the receptor vegetables, such as Chinese cabbage, lettuce, and red radish, were treated with aqueous extract obtained from Sajabalssuk (A. sp*I), Ssajuarissuk (A. sp*II) or Ssajarissuk (A. sp*III), their germination rate, leaf number, plant height, and root length were restricted with increasing concentration of aqueous extract. Allelopathic effect was the highest in radish, than lettuce and Chinese cabbage in order. The growth of topplant were more inhibited then root growth observing in restriction of plant height, root length, and chlorophyll contents. The plant height, the root length of red radish were 53.3 and 61.2% and their fresh weights were 19.8 and 26.4% compared to those of controls, respectively. A. sp*III showed the highest allelopathic effect among the donor plants. In HPLC analysis, 7 phenol compounds were identified in A. sp*I and A. sp*II, and, in A. sp*III, and hydroxybenzoic acid and phenylacetic acid were further identified as allelochemicals. It is considered that their plant growths were variously inhibited by the amounts and types of allelochemicals in aqueous extracts. To increase the productivity of farm land after cultivation of mugwort, these results can be useful to select the following field crops.

Oryzalexine A, a potent growth inhibitor against several weeds such as Digitaria sanguinalis (L) Scop., and Amaranthus lividus L. was purified by conventional solvent partitioning and column chromatographies. This substance showed strong inhibitory activity on several weeds: Germination of seeds of Poa annua L. was inhibited by 36.5 % at 1.0 mM and Amaranthus lividus L. by 56.1% at 2.5mM. Growth of root and shoot of Digitaria sanguinalis (L.) Scop. by 10.2% and 22.4% at 2.5mM, respectively. These suggest that Oryzalexine A in rice straw might affect the germination and growth of susceptible weeds and other plants.

본 실험은 포장에서 주로 발생하는 velvetleaf등을 비롯하여 7종류의 잡초를 마른 상태와 생체상태로 각각 수확하여 지상부와 지하부로 나누어 물질을 추출 이들을 alfalfa 종자 발아와 생육정도 검정에 사용하여 지상부와 지하부의 생육억제정도를 비교하였으며 이 중 억제효과가 제일 큰 velvet-leaf의 추출물은 여러 농도로 silica sand와 혼합하여 alfalfa에 대한 타감작용을 검토하였다. 1. 7종류 잡초의 지상부와 지하부의 추출물 처리는 alfalfa의 발아율, 발아세, 유근의 길이, 무게 등을 억제시켰다. 지상부와 지하부의 억제효과를 비교하면 지상부의 추출물이 지하부의 추출물 보다 더 큰 타감작용을 보였으며, 이 중에서 velvetleaf 추출물이 가장 억제적이었고, crab-grass 추출물은 가장 낮은 억제 정도를 보였다. 또 alfalfa 발아와 유근의 생육에 대한 velvetleaf의 건조 추출물과 생체 추출물을 비교하면 건조 추출물이 더 억제적이었다. 2. 농도에 따르는 velvetleaf 추출물 처리에서는 농도가 증가할수록 alfalfa 발아율, 유근의 길이, 무게 등이 대조구와 비교하여 상대적으로 더 억제되었다. 3. 건조된 velvetleaf 잔기를 silica sand와 혼합처리에서 잔기의 비율이 증가 될수록 alfalfa의 출현율과 생존율이 더 억제되었으며 그 정도는 잔기 비율 1%에서 가장 억제적이었다. 4. 잡초의 추출물과 잔기의 처리는 alfalfa의 발아와 생육에 억제적으로 작용 하여 타감작용이 인정되었다.