시설재배농가 확대 및 농약허용물질목록관리제도 시행으로 농업해충 방제를 위한 천적활용은 향후 늘어날 것으로 예상된다. 시설작물 재배시 발생하는 주요해충은 진딧물류, 잎응애류, 가루이류, 총채벌레류 등으로 4그룹 이상의 해충이 작물 재배기간 내 혼재되어 발생하고 있다. 이들을 방제하기 위하여 천적 활용시 밀도 유지를 위한 잦은 방사는 농가가 사용을 기피하게 만드는 주요 요인이 되고 있다. 이러한 문제를 해결하기 위하여 천적을 적극 활용하는 네덜란드를 중심으로 시설온실에서 다포식성천적 등을 적절하게 사용할 수 있는 방안으로 해충발생전 천적 방사, 대체먹이 활용 등을 이용하고자 하는 연구가 이루어져 왔으며, 본 발표를 통해 국내에 활용 가능한 천적 연구에 대한 정보를 공유하고자 한다.

2017년부터 2018년까지 전북지역 오디 생산용 뽕나무에 발생하는 주요 해충의 발생 양상과 유기농업자재에 의한 뽕나무이 방제 효과를 조사하였다. 부안과 익산지역을 중심으로 수확 전 발생하는 주요 해충은 뽕나무이, 미국흰불나방, 뽕나무명나방, 노린재류, 꽃매미 6종이었으며 수확 후에는 미국흰불나방, 뽕나무명나방, 총채벌레, 미국선녀벌레, 노린재류 등 5종이 발생하였다. 또한 오디 생산용 뽕나무 재배시 가장 문제가 되는 병해충인 균핵병과 뽕나무이를 동시에 친환경적으로 관리하기 위해서는 데리스 추출물(데리스 90%) 250배와 황토유황 500배를 혼합하여 처리하는 것이 방제효과 97.6%로 효과적이었다. 선발된 유기농업자재에 효과적인 처리시기는 4월 상·중순이며, 7일 간격으로 3회 방제하면 94% 이상의 방제효과가 있고 약해도 발생하지 않아 유기농업자재를 이용한 뽕나무이의 친환경방제가 가능할 것으로 판단되었다.

Insecticide Resistant Management (IRM) and Positive List System (PLS) is the main keyword in current insect pest management. So we are trying to develop a integrated insect pests control prescription issuing system based on big data which linked National Crop Pest Management System (NCPMS). To manage the insecticide resistance, IRAC suggest some tips for effective control : choosing the effective insecticide and alteration of these insecticides based on MOA. Then, how to distinguish the effective or non-effective insecticide. Until now, bioassay is the right answer. However that is time-consuming, labor-intensive and costly process. So we have been steadily advancing molecular based insecticide resistance diagnosis for supporting and/or substitution of classical bioassay. And it will link to PLS. Here we discuss more detailed methodology of molecular based insecticide resistance diagnosis and its application for the insect pests control prescription issuing system.

드론을 이용한 배추 해충 방제기준을 설정하기 위하여, 드론의 살포높이(3, 4, 5 m), 진행속도(3, 4 m/sec) 에 따른 하향풍 속도, 살포 폭, 그리고 낙하 입자수와 입자크기를 감수지를 이용하여 조사하였고, 항공방제용 농약 4종을 이용하여 배추 주요해충인 배추좀나방, 파밤나방, 담배 거세미나방에 대하여 완전치사농도와 약량을 실험실에서 검정하였다. 드론의 농약 살포시 면별 낙하입자비율은 표면 80.5, 수직면 14.8, 밑면 4.7%였고, 살포높이에 따른 낙하입자수는 3 m = 53, 4 m = 40, 5 m = 39개/cm2였다. 비행속도별 낙하입자수는 3 m/sec = 62, 4 m/sec = 25 개/cm2였다. 실내시험에서 배추좀나방의 완전치사농도와 치사량이 클로르페나피르액상수화제(20배, 0.5 μl) 비스트리플루론 ․ 클로르페나피르 액상수화제(25배, 0.5 μl)였다. 파밤나방에 대하여는 클로르페나피르액상수화제(20배, 1 μl), 비스트리플루론 ․ 클로르페나피르액상수화제(20 배, 1 μl)였고, 담배거세미나방에 대하여는 클로르페나피르액상수화제(20배, 1 μl), 비스트리플루론 ․ 클로르페나피르액상수화제(20배, 0.5 μl)였 다. 따라서 드론을 이용하여 배추 주요해충을 방제하는 방법으로 클로르페나피르액상수화제 또는 비스트리플루론 ․ 크로르페나피르액상수화제 를 20배액으로 희석하여 3 m 높이에서 3 m/sec 속도로 살포하면 72개/cm2의 농약입자가 낙하하므로 해충방제에 효과적일 것으로 판단된다.

The purpose of this study was to examine the damage rate of insect pests on chestnut orchards. and to investigate the effects of varying methods of insect pests control. The results revealed that there was a statistically significant(p<0.05) difference among early-ripenign, middleripening, and late-ripening cultivars. The damage rate of Dichocrocis punctiferalis was highest in early-ripening cultivars(24.22%), followed by middle-ripening(22.71%) and late-ripening(20.25%). The damage rate of Curculio sikkimensis was highest in late-ripening cultivars(24.52%), followed by middle-ripening(22.61%) and early-ripening(19.96%). There was a statistically significant(p<0.05) difference in damage rates after insect pests control. Two methods of insect pests control, using either helicopter or an unmanned drone, were used. The results revealed significant differences (p<0.05) between D. punctiferalis and C. sikkimensis. Changes in the damage rates of D. punctiferalis after drone control were 8.97%~9.62%, and that after helicopter control was 14.82~15.94%, which was 20.25%~24.22% significant difference(p<0.05) from control site. Changes in damage rates of C. sikkimensis after drone spraying was 6.77~7.72%, and that after helicopter spraying was 12.62~14.94%, which was 19.96~24.52% significant difference(p<0.05) from control sites. Control effects of D. punctiferalis by drone spraying was 59.06% and helicopter spraying was 30.82%. Control effects of C. sikkimensis by drone spraying was 66.97% and helicopter spraying was 40.78%.

이중가닥 RNA (double-stranded RNA, dsRNA)는 표적 유전자의 발현을 억제하는 기능으로 해충방제에 응용되었다. 인테그린은 α와 β 단위체로 구성된 이량체 막 단백질이다. 진핵생명체에서 인테그린은 세포-세포 및 세포-세포외기질의 상호연결에 중요한 역할을 담당한다. 인 테그린 β 단위체 발현을 억제하는 특정한 dsRNA (= dsINT)는 해당 곤충에 뚜렷한 치사효과를 유발한다. 또한, dsINT를 발현시키는 형질전환 된 대장균도 해당 곤충에 뚜렷한 살충력을 가진다. 그러나 이 세균 살충제의 야외 적용을 위해서는 제형화 기술이 필요했다. 본 연구는 dsINT를 발현하는 재조합 세균을 동결 건조시켜 대상 곤충에 대해 살충효능을 검정하였다. 동결 건조된 세균은 파밤나방(Spodoptera exigua) 종령 유충에 높은 섭식독을 일으켰다. 파밤나방에 대해서 Bacillus thuringiensis 상용 살충제 처리는 불과 60%의 살충력을 보이는 반면, 동결 건조된 dsINT 발현 세균과 혼합 처리할 때 살충력은 크게 증가하였다. dsINT 발현 세균은 해당 인테그린 염기서열 유사성에 따라 차이를 보이는 해충 종에 선 택적 독성을 나타냈다. 이 결과는 인테그린에 특이적 dsRNA를 발현하는 세균이 동결 건조 제형화 조건하에서도 살충력을 유지한다는 것을 나타 냈다.

Insect pests on cruciferous crops and their natural enemies were surveyed during 2009~2011 in Daegwallyeong highland region which has been famous for summer Kimchi cabbage production in Korea since 1970s. Totally 15 insect pests have been reported in cabbage field in Daegwallyeong. Diamondback moth (DBM, Plutella xylostella) imported cabbage worm (Artogeia rapae L.), cabbage armyworm (CAW, Mamestra brassicae L.), cabbage looper (CL, Trichoplusia ni), cabbage sawfly (Athalia rosae ruficornis Jakovlev), and turnip aphid (Liphapis pseudobrassicae (Davis)) were the dominant pest species among the 15 species. For monitoring as well as controlling insects with weak flight, yellow sticky cards (10×15㎝) were used in cabbage fields. The sticky cards were hanged on plastic stick and placed at a 2-5 m distance within cabbage field (1,000㎡). Sex pheromone traps were employed for monitoring and controlling insects with strong flight. The survey result showed that there was significantly reduced pest occurrences in treated, compared to untreated condition. Similarly, The results from the sex pheromone traps were coincident with those of sticky trap. DBM, CAW and CL were found less in the treated than untreated fields; by 67.5%, 70.6% and 44.0%, respectively. Although the control efficacy of such traps was less than that of chemical spray, the use of sticky card trap combined with sex pheromone trap could be useful management tools for controlling insect pests in cabbage fields.

There are relatively few cases of classical biological control in Korea. During 1904 to 1905 the wooly apple aphid, Eriosoma langigerum, had been happen to invade into Korea from Japan together with imported seedling of apple and its damage spread to Jinampo near Pyoung-Yang City and it occurred all across the country by 1934. In 1934 a parasitic wasp, Aphelinus mali, was introduced and released to apple orchards and found established in nature. And it was the first case of classical biological control in Korea. Afterward couple of classical biological control programs against invaded pests were conducted mainly by the RDA with variable results. For example the orange scale, Ceroplastes rubens, and the Eggar which were successfully controlled by introduction Anicetus beneficus and Thecodiplosis pinico, respectively.

This study was conducted to develop the biological insect pest control on pear orchard by using the native natural enemy from 2011 to 2013. We selected the green lacewing, Chrysoperla nipponensis (Okamoto) for useful natural enemy and developed the artificial mass rearing technique, and tested finally in pear orchard located in Anseong city. Nine species of insect pests including Pseudococcus comstocki on pear and seven species of natural enemies including C. nipponensis were investigated in Gyeonggi area. The optimum developmental temperature, humidity and photoperiod for C. nipponensis were 20 ∼25℃, 60∼70%, and 16L : 8D respectively. One C. nipponensis during larval stage could feed about 440 2nd nymphs of P. comstocki at 20℃. The more prey density increase, the more feeding amount C. nipponensis larva was increased. The eggs of almond moth, Ephestia cautella were useful as a substitute diet for natural enemy, C. nipponensis. Three commercial insecticides which registered as insecticide on pear shown the low toxicity to natural enemy, C. nipponensis will be suitable for integrated pest management (IPM) in pear orchards. C. nipponensis shown the about 80% of control value against the P. comstocki in field test when had committed from the early May to the early July. Controlling the P. comstocki by using of C. nipponensis, not only the 30% of yield was increased, but also the quality especially fruit sugar was increased.

This study reports the results of natural enemy application against four insect pests on greenhouse sweet pepper in winter cropping system. Orius laevigatus (3.3/㎡) was released at two different times (Nov. 2005 & Feb. 2006) to control Frankliniella occidentalis, western flower thrips (WFT). Throughout the cropping season, the lowest level recorded of WFT was less than 2.0 individuals per yellow sticky trap. Amblyseius swirskii (232.3/㎡) was released four times (Nov. 2007 ~ Aug. 2008) to control Bemisia tabaci, tobacco whitefly (TWF). Until July 2008, TWF population had been suppressed lower than 6.6/trap. Phytoseiulus persimilis (44.5/㎡) was released seven times (Apr. 2008 ~ Aug. 2008) to control Tetranychus kanzawai, tea red spider mite (TRSM). As a result, TRSM population was suppressed lower than 1.7/leaf. To control aphids, Aphidius colemani (9.5/㎡) was released seven times (Oct. 2004 ~ Jan. 2005), with the transplantation of banker plants (5.5pot/660㎡). As a whole, aphid"s population has been successfully reduced to less than 2.0/leaf.

Dynamics of prey/host and predator/parasitoid interaction has been the main concern of the population ecology during the second half of the 20th century. Many theoretical and experimental studies have been conducted and discussed. The main questions were whether the interaction could be in stable state and whether the parasitoid/predator could regulate the host/prey populations, and if so at what levels. The questions are not yet solved, but some hypotheses have been presented that the prey/predator interaction could be in stable state, when both the predator and prey population are under intraspecific competition so that both populations could be limited at a low levels. This prerequisite, however, could not be easily satisfied in nature and several hypotheses have been discussed up to date. Biological control of pests, which is based on the predator-prey system dynamics, has not always been successful, because we do not understand the systems sufficiently. Thus, most successful cases of the biological control were dependent upon ‘trial and error’. But as the predator-prey system dynamics could be well understood in the future, more systemic control program of biological control could be possible. In this review, we analyzed the studies for biological control of pests in Korea based on 164 cases reported in the last forty years and will consider the future goal of the studies for more reliable control programs.

Biological-control-based-integrated-pest-management of major pests occurring on sweet pepper in greenhouse during summer season was tried. As many as 2.1 Orius laevigatus per ㎡ were released in two times on June 6 and 19, and the population of thrips was kept under control and accordingly the damage was negligible throughout the season. To control aphids, a total of 0.8 Aphidius colemani per ㎡ were released in four times, 0.2 of them at a time, flonicamid on May 14 and July 18 and pymetrozine on June 14 and September 4 were sprayed on the spots of high aphid occurrence to reduce the release of the wasp, and the density of aphids was kept under control. Whitefly was controlled successfully by releasing a total of 343.4 Amblyseius swirski per ㎡ in nine times, 38.1 of them at a time, from May 9 until November 12 and dinotefuran was sprayed on November 12 when the density of whitefly increased up to 200 per trap. Tetranichus kanzawai was controlled by both Phytoseiulus persimilis which was released a total of 44.4 per ㎡ in five times 8.9 of them at a time from May 23 to September 10, and the A. swirski which was released for the control of whitefly.

Methyl bromide (MeBr) which is widely used in most of the countries for quarantine purpose, has been designated and under control as ozone depleting substance. This study is on the toxicity of ethanedinitrile as a MeBr alternative to control several wood-related insect pests. LC50 value of Ethanedinitrile(EDN) for Reticulitemes speratus, Tomicus piniperda adults at 21℃ was 0.26, 4.83 mg/L respectively. LC99 value of EDN for R. speratus at open status and closed wooden cubes was 0.65, 0.62 mg/L respectively, whereas MB was 13.96, 31.10 mg/L, which means that the toxicity of EDN for R. spratus is greater and EDN penetrates better into wood as compared with MB. The morality of EDN 20.3 mg/L(6hr) at 25℃ for Crytogenius lutats in wood was 90.3% in larvae, 58.6% in pupae and 89.5% in adults, which means that pupae were more resistant than larvae and adult. All stages of C. lutats were disinfected with EDN at or more than 29.9.mg/L, 6hr(CT product 89 mg hr/L) at 25℃. In sorption and application test, CT product of EDN 50 g/㎥(6hr) was 157 g hr/㎥ in the conditions of 21% wood moisture content with 25% loading, 171 g hr/㎥ in 54% wood moisture content with 25% loading and 158 g hr/㎥ in 54% wood moisture content with 50% loading, respectively. In all treatments, adults of Cryphalus fulvus and R. speratus were completely killed. The minimal CT product (158 g hr/m3) of 6hrs treatment was higher than CT product to disinfect C. lutats (89 mg hr/L). It is expected that it can be used as alternative fumigant of MeBr for woods.

The devastating insect pests of the tomato (Lycopersicon esculentum) were surveyed in environmentally friendly (no-pesticide and organic) greenhouses located in the provinces of Gyeonggi, Gyeongsang, Gangwon, Chungcheong and Jeju. Whitefly, leaf miner, aphid and moth were recorded as the major pests in these surveys. Among them, whitefly was the most serious pest of greenhouse cultivated tomato and major species was Trialeurodes vaporariorum. The population densities of T. vaporariorum was 0~36 nymph per leaf. Population of moths Spodoptera litura were the highest in summer season. Population of the whitefly was considered to be influenced by the previous cultural methods. Farmers in organic cultivation, used insect natural enemy, commercial EFAM (Environment Friendly Agricultural Material), yellow sticky trap or home made pesticides. Commercial microbial pesticides were applied to evaluate their insecticidal activities against whitefly. The results revealed that microbial pesticides exhibited 65.3 to 100% whitefly nymph mortality. Further, pesticidal activities of Cooking Oil and Yolk (COY), plant extracts and neem oil were evaluated against other tomato pests as well.

A new Bacillus subtilis isolate showed high anti-fungal activities (more than 80% control efficacy) against several plant diseases such as rice blast (Magnaporthe grisea), tomato gray mold (Botrytis cinerea), tomato late blight (Phytophthora infestans) and wheat leaf rust (Puccinia recondita). We tried to confer an insecticidal activity to this B. subtilis isolate for constructing a recombinant strain which has dual functions, anti-fungal and insecticidal activity. The insecticidal cry1Ac gene of B. thuringiensis was constructed under its own promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K-1Ac). The plasmid, pHT1K-1Ac was introduced into B. subtilis isolate by electroporation and the transformant was confirmed by PCR with cry1Ac specific primers. B. subtilis transformant produced a parasporal inclusion in the cells as in B. thuringiensis and the size of that protein was appox. 130 kDa. The insecticidal activity of the transformant was checked against lepidopteran pest, Plutella xylostella. This result suggests that this recombinant B. subtilis strain shows the possibility of controlling harmful insect pests as well as plant fungal diseases simultaneously at one crop, and both culture broth and harvested cells of this strain can be used as individual biological control agents separately for integrated crop protection.