The objective of this study was to examine effect of ethylene glycol for semen cryopreservation in Korean Jeju Black Bull. The semen was cryopreserved with extenders containing cryoprotectants (7% glycerol and 3%, 5%, 7% ethylene glycol) and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 min, 5 cm for 10 min and 8 cm for 10 min. And then frozen straw was plunged into LN2. Post-thawed sperm motility, viability and membrane integrity were significantly higher in 5% ethylene glycol (72.5±5.00%, 54.88±0.66% and 46.00±2.40%; p<0.05). Motility and viability were similar between 7% glycerol and 5% ethylene glycol. However, the membrane integrity was significantly higher in 5% ethylene glycol (34.69±4.64% vs 46.00±2.40%; p<0.05). The viability and membrane integrity were significantly higher in 5 cm for 10 min and 8 cm for 10 min than 3 cm for 5 min (viability: 55.81±2.94, 55.19±3.34 vs 47.94±3.48%; p<0.05 and membrane integrity: 44.94±3.51, 46.06±2.25 vs 40.38±1.03%; p<0.05). The percentage of capacitated sperm assessed by CTC staining, percentage of F pattern was higher in 7% glycerol, 5% and 7% ethylene glycol, and AR pattern was significantly higher in 3% ethylene glycol. F pattern was significantly increased in 5 cm for 10 min and 8 cm for 10 min (p<0.05), but AR pattern was significantly increased in 3 cm for 5 min (p<0.05).

This study was carried out to establish most suitable freezing condition, to evaluate the different glycerol concentration of freezing and thawing rates on motility, viability, membrane integrity and acrosome intecrity of frozen Korean Jeju Black Bull spermatozoa, Semen was collected from a Korean Jeju Black Bull using an artificial vagina and transported to the laboratory. The semen was extended gradually 1:5 then cooled slowly for 2 hrs to 4. The semen was diluted 1:1 with cryoprotectant extenders (3%, 5% and 7% glycerol) and equilibrated for 2 hrs at cold chamber and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 min and above 8 cm for 10 min. And then the frozen straw was plunged into LN. The presented straws were examined the viability and motility after thawed at 37 water bath. The viability and membrane integrity immediately post-thawing were significantly higher in samples frozen in 7% glycerol than 3% and 5% glycerol (p<0.05). After CTC staining to assess acrosome integrity, F pattern was significantly increased, but B pattern was significantly decreased in 7% glycerol (p<0.05). Freezing distance of 5 cm from liquid nitrogen and pre-cooling for 10 min yield better survival and membrane integrity, but not significant difference. However, AR pattern according to CTC staining was significantly decreased in 3 cm for 5 min.

폴리머 시멘트 고화체는 일반 몰타르 내의 시멘트 수화물을 폴리머 개질제를 이용하여 부분적으로 대 체함으로써 그 기능을 강화시킨 복합재료로써, 특히 시멘트 몰타르에 폴리머를 첨가하는 것은 그 화학적 내구성을 향상시킨다고 알려져 있다. 따라서 본 연구에서는 고화재료로서의 폴리머 시멘트에 대한 낮은 침투성 및 낮은 이온 확산도 등과 같은 향상된 화학적 내구성을 확인하기 위하여 폴리머 시멘트 시편들을 제조하였다. 이때 폴리머의 함량은 0에서부터 30%까지 변화시켰으며, 물에 대한 시멘트 비(W/C)를 33%와 50%로 각각 유지 시켰다. 충분히 경화시킨 후에, 제조된 시편들에 대한 구조적 건전성을 압축강도와 수침법에 의한 공극도를 통하여 평가하였다. 그 결과, W/C 비가 33%이고, 폴리머 함량이 약 10%인 폴리 머 시멘트 시편에서 가장 향상된 개질변화를 얻을 수 있었다. 끝으로 이 최적의 조합비를 가지는 시편에 대하여 ANS 16.1에 따르는 침출시험을 수행하였으며, 그 결과를 일반 시멘트 고화체와 비교하였다.

인간은 누구나 자연으로의 回歸와 名利를 추구하는 현실 安住의 矛盾된 개념 이 意識속에 內在해 있다. 인간이 世慾을 버리기는 쉽지 않지만 超然히 마음을 비우고 歸去來의 삶을 실천한 사람은 전설 속의 許由, 춘추시대 吳나라의 季札, 後漢光武帝의 벗 嚴子陵, 東晉의 陶淵明, 우리나라의 元天錫(1330～?), 南道振 (1674～1735), 李夏坤(1677～1724) 같은 분들이 있다. 麗末三隱의 한 분인 吉冶隱도 高麗의 滅亡이라는 時代狀況에서 隱逸의 삶을 선택한 분이다. 冶隱의 文學이나 思想, 節義등에 관해서는 이미 여러분의 論稿 가 나와 있고, 야은의 思想的·節義的측면을 살피면서 文學的특성을 살핀 論議 의 방향도 크게 다르지는 않다. 本稿의 목적은 麗末三隱에 대한 再檢討와, 冶隱의 文學에 나타난 삶과 隱逸의 정신적 배경을 살피는 데 있다. 아울러 새로 발견된 冶隱詩稿를 소개하는 것도 이 논문의 목적 중 하나다. 論旨를 전개하면서 그때그때 先行硏究의 성과를 살피되 이미 잘 알려진 내용은 再論하지 않고, 本稿에 꼭 필요한 부분들만 살피겠다.

Structural property in communities of aquatic insects and benthic macroinvertebrates could reflect the impact of environment on aquatic ecosystems. Benthic macroinvertebrates were collected at 18 different lakes across different levels of environmental impact in Korea. Community responses were analyzed by species abundance distribution (SAD). Rank-abundance (or relative abundance) curves were evaluated by classical SAD (Geometric series, Log-series, and Lognormal distribution) and niche apportionment models (dominance pre-emption, random assortment, random fraction, and dominance decay). Geometric series was more accepted in lakes among classical SAD models. Niche apportionment models were observed in a diverse manner across different level of ecological integrity in lakes. Dominance decay model and random fraction model were frequently fitted to the lakes with higher integrity, while dominance pre-emption and random assortment models tend to be presented in low to middle range in integrity.

The missiological tension with regard to the question of salvation and humanization is dynamic, and it is found in the different perspectives between the WCC camp and Lausanne camp or evangelical and ecumenical camps on the mission. Although the naps are getting narrowed thanks to the holistic missiology, the tension still exists in the Korean context, of which example is the CCK’s case that raised objection against hosting 2013 WCC conference. This paper is, focusing on the holistic feature of humanization and salvation, to provide a missiological base to overcome the problem caused by the discrepancy between K. Barth’s “God’s humanity” and Korean Christians’ inhumanity. It analyses style and patterns of criticism on Korean Christians, and argues that the culprit which causes Korean Christians' humanity to be criticised negatively is Christ-centered and Church-centered attitude of the Korean church, which is Korean evangelical camp’s theological stance. It points out that this is caused by lack of “God’s humanity” which helps Christians remain faithful to their own identity and mission. Therefore it suggests that this problem is solved when Christians acknowledge their own raison d'etre to realize God's humanity, and that by so doing the holistic balance between salvation and humanization is to be achieved. It also suggests ecological system and internet as a sample of holistic mission field which makes the interdependent mission possible.

The beneficial effect of glycerol as a cryoprotectant, especially for sperm cryopreservation, has been shown in many studies. However, glycerol is toxic to living cells, and boar sperm in particular show greater sensitivity to glycerol than sperm from other domestic animals. Amides have been studied as alternative cryoprotectants for freezing stallion sperm. Sperm frozen in methylformamide or dimethylformamide as cryoprotectants show similar motility when thawed compared with sperm frozen in glycerol. We evaluated the cryoprotective effects of dimethylformamide on boar sperm freezing. To test the effect of amides, the concentration of boar semen was adjusted to , and seminal plasma was removed using Hulsen solution. After centrifugation, the pellet was diluted in modified-Modena B extender. Lactose-egg yolk (LEY) extender was used as the cooling extender. The freezing extender was madeed aaddition of the optimal amount of glycerol and amides to LEY-Glycerol-Orvus ES Paste extender, and this extender was used for the second dilution. Diluted sperm were frozen in liquid nitrogen using the 0.5 mL straw method. Sperm frozen in extender with glycerol as a cderol were compared with those frozen in extender including the different amides. Sperm were tested for motility, viability, the sperm chromatin structure assay, and normal apical ridge after thawing. The percent of motile sperm diluted in glycerol was as high as that in the stallion study (61%). Dimethylformamide showed positive effects on sperm quality and was better than glycerol. Methylformamide provided similar sperm quality as glycerol. Therefore, dimethylformamide is useful for reducing cryoinjury in boar sperm and is expected to be useful as an alternative cryoprotectant.

The objective of this study is to evaluate the mechanical behaviors and structural integrity of the weldment of high strength steel by using an acoustic emission (AE) techniques. Monotonic simple tension and AE tests were conducted against the 3 kinds of welded specimen. In order to analysis the effectiveness of weldability, joinability and structural integrity, we used K-means clustering method as a unsupervised learning pattern recognition algorithm for obtained multi-variate AE main data sets, such as AE counts, energy, amplitude, hits, risetime, duration, counts to peak and rms signals. Through the experimental results, the effectiveness of the proposed method is discussed.

The objective of this study was to determine the potential hazardous effects of sorting process by flowcytometry on the quality of boar spermatozoa by flowcytometer. Freshly collected boar semen was diluted and divided into two groups; control none sorted and sorted. Sperms in sorted group were processed with flowcytometer for cell sorting with 100 uM nozzle under the 20 psi pressure. Measurements on each parameter were made at two time points, 0hr (right after sorting) and 24 hr post sorting. Although there was a tendency of lower viability in sorted group than none sorted control group, the percentage of live cells in control(75.83+-6.92 & 59.53+-10.34) was not significantly different from sorted (59.7+-7.34 & 43.97+-3.76) at both 0 and 24 hr post sorting. However, sorted sperm showed significantly lower mitochondrial function compared to the control at both 0 h (79.37+-3.22 vs. 63.50+-10.05) and 24hr(67.27+-3.22 vs. 46.97+-5.37) time points (p<0.007). Sperm DNA fragmentation rate was significantly lower in control (22.0+-7.04) than that of sorted (32.27+-7.49) at 24 hr time point (p<0.0002). Taken together, these data suggested thatsorting process by flowcytometer may have influenced sperm motility rather than viability. Also high speed sperm sorting by flowcytometer has significant effects on DNA fragmentation on elapsed time after sorting.

The objective of this study is to evaluate the mechanical behaviors and structural integrity of the weldment of high strength steel by using an acoustic emission (AE) techniques. Simple tension and AE tests were conducted against the 3 kind of welding test specimens. In order to analysis the effectiveness of weldability, joinability and structural integrity, we used K-means clustering method as a unsupervised learning pattern recognition algorithm for obtained multivariate AE main data sets, such as AE counts, energy, amplitude, hits, risetime, duration, counts to peak and rms signals. Through the experimental results, the effectiveness of the proposed method is discussed.

The objective of this study was to establish the optimal conditions for hypoosmotic swelling (HOS) test to assess the functional integrity of the membranes of boar fresh or frozen/thawed spermatozoa. When pooled semen sample was incubated for 30 min at 37℃ with different test solution of varied osmolarity, the highest percentage of HOS positive spermatozoa was observed in a 150 mOsmol fructose/Na-citrate solution (33.6%). Incubation time did not affect significantly the score of HOS positive spermatozoa observed in a 150 mOsmol fructose/Na-citrate solution at 37℃, but the osmolarity affected the score of HOS positive spermatozoa under the same condition above. Fresh semen was significantly better than frozen/thawed semen in semen parameters evaluated such as motility, viability, membrane integrity and lipid peroxidation (p<0.05). In the relationships of sperm parameters, motility vs viability, motility vs membrane integrity and viability vs membrane integrity were positively correlated (0.82～0.94) but lipid peroxidation vs other estimated factors was negatively correlated (－0.90～－0.98). Among the evaluation methods, motility vs viability, motility vs membrane integrity and lipid peroxidation vs other estimated factors were significantly correlated (p<0.05). These results of this study indicate that the optimal condition of HOST in boar spermatozoa is a 150 mOsmol fructose/Na-citrate solution for 30 min incubation at 37℃ and HOST can substitute the examination of motility, viability and lipid peroxidation.

Hypoosmotic swelling test (HOST) is used for evaluating the plasma membrane function and fertilizing ability in mammal spermatozoa. However, HOS solutions and experimental conditions have not been determined clearly for assessing canine spermatozoa. This study was conducted to examine the HOS solutions and assay conditions, including incubation time (30 to 120 min), storage temperature (4, 17 and 20℃), semen status (fresh and frozen). Maximum spermatozoal plasma membrane swelling was obtained in an 150 mOsm Na-citrate/Fructose solutions with an incubation time for 45 min. The storage temperature and semen status affected the percentage of HOS positive spermatozoa. The HOS test adapted to canine spermatozoa in this study was simple and highly consistent assay with good repeatability. The optimal condition of HOST in canine spermatozoa is an 150 mOsm Na-citrate/Fructose solutions with an incubation time for 45 min regardless of semen storage temperature and semen status.

This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than 80% of fresh sperm washed with mTLP-PVA medium at 20℃ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane (36.4~46.9%) and nonfunctional mitochondrion (55.1~71.1%) in the mTLP-PVA and BTS washing media at 20℃. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at 4℃ washing temperature than at 20℃ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

철근콘크리트 구조물의 보수ㆍ보강 등의 유지관리를 위해서는 내구성과 내하성을 동시에 고려한 건전성평가의 의사결정기준이 절실히 요구된다. 본 논문은 CART-ANFIS을 사용하는 철근콘크리트 구조물에 대하여 효율적인 모델을 나타내었다. 철근콘크리트 구조물의 손상과 진단 등에 활용되어온 분류형 전문가시스템의 일종인 퍼지이론을 이용한 결정목 구조와 기존의 인공신경망을 이용한 결정목 구조의 건전성평가를 비교 분석한다. 손상된 철근콘크리트의 내구성 회복을 위한 보강설계 이론과 내하력 증가를 위한 보장설계 이론을 정립시켜 손상검출의 산정식을 유도하였다. 본 연구의 건전성 평가시스템 모델을 이용함으로서 보다 효율적인 철근콘크리트 유지관리 뿐만 아니라 생애주기비용 예측을 수행 할 수 있다.

본 연구에서는 토마토 종자의 priming 및 발아과정 중 세포막의 기능이 종자활력에 미치는 영향을 구명하고자 하였다. 토마토 종자의 적정 priming 처리제는 150 mL의 KNO3였고, priming 처리된 종자는 발아촉진에 유효하였으며, 그 효과는 저온에서 현저하였다. KNO3로 priming은 처리과정중 처리제에서 분리된 이온이 종자내로 이동하였다. Priming 처리과정중 전기전도도는 발아속도 단축에 가장 효과적이었던 KNO3에서 처리개시 직후 약간 낮았다가 그 후 처리최종일까지 일정한 수준을 유지하였다. 발아기간중 용액의 전기전도도는 KNO3 용액으로 priming 처리된 종자에서는 낮았으나, K3PO4 용액으로 priming 종자에서는 높게 나타났다. 발아촉진에 가장 효과적이었던 priming 처리제인 150 mL의 KNO3 용액으로 priming 처리하면 처리과정중 단백질, 아미노산, 가용성 당의 유출량은 K3PO4 및 침지종자에 비해 낮았으며, 그 효과는 발아시에도 유지되었다.