The ant species, Vollenhovia emeryi, is distributed in Far East. The species can be divided into two major groups by their wing morphology of reproductives: short-winged and long-winged. A nationwide survey of the species was conducted for analyzing the mitochondrial haplotype diversity and genetic population structure. We collected 91 samples from 40 locations. A total of the 1239 bp partial COI (cytochrome C oxidase 1) region was used for the analyses. We found the total of 21 haplotypes. The mitochondrial haplotypes may correspond to the wing morphology. The genetic population structure examined potential geographic barriers of gene flow such as distance, mountains, rivers and plains which are non-mountain areas to prevent dispersal through mountain range. The result implied that no barriers considered in this study affected differently gene flow. Therefore, the behavioral characteristics of the ant may be the causal constraint of its genetic exchange.

본 연구는 자란개체군을 식물사회학적 방법으로 분류하고, 식생과 환경과의 상관관계를 밝히고자 분포서열법을사용하여 분석하였다. 자란개체군은 노간주나무 우점개체군, 산철쭉 우점개체군, 붉나무 우점개체군, 자란 전형개체군으로 분류되었다. 자란개체군은 주로 한반도의 남서해안을 따라 분포하고 해발고 4-40m의 해안가 낮은 사면의 평균 41.7˚의 다소 급한 경사지에 위치하고 있다. 토양의 이화학적 성분을 분석한 결과 유기물함량은 2.38-6.70%, 전질소함량 0.09-0.27%, 치환성 K 1.08-1.72cmol+/kg, 치환성 Ca 3.56-7.71cmol+/kg, 치환성 Mg 1.52-3.21cmol+/kg, 양이온치환용량 5.28-16.95cmol+/kg이며, 토양 pH는 4.60-6.01인 것으로 조사되었다. 산철쭉 우점개체군은 경사가 다소 급하고 토양내 양이온치환용량과 pH, 전질소, 유기물함량이 가장 높은 지역에 분포하였다. 붉나무 우점개체군은 경사가 다소 완만하고 양이온치환용량, pH, 전질소, 유기물함량이 상대적으로 낮은 지역에 분포하는 것으로 나타났다. 그리고 노간 주나무 우점개체군과 자란 전형개체군은 중간입지에 분포하는 것으로 나타났다. IUCN의 평가기준에 따라 취약종으로 지정된 희귀식물인 자란은 자생지가 해안가를 따라 분포하는 개체군의 생육특성 상 개발로 인한 훼손으로부터 자생지 의 보전을 위한 구체적인 대책 마련이 요구된다.

The soybean aphid, Aphis glycines Matsumura, was recently introduced from Asia into North America (NA) where it has become a serious pest of soybeans. This invasive pest has rapidly spread throughout the midwestern United States and southern Canada since 2000. We examined 585 individuals obtained from 23 different collections in USA, Korea, China, and Japan using eight microsatellite loci. Based on analysis of multilocus genotype, gene diversity and number of alleles in NA were averaging 0.40 and 2.70, whereas in Asia averaging 0.55 and 4.32, respectively. The factorial correspondence analysis displayed that some Korean populations were closely related to the NA populations. Structure analysis resulted in two conspicuous clusters, NA and Asia, as the most likelihood number of clusters (K). Bayesian assignment tests revealed that Osan and Milyang populations were most likely assigned to the NA populations. Bottleneck test did not show significance of genetic bottleneck in all populations. We also discuss the invasive history of the soybean aphid in light of population genetics.

본 연구는 제주도 용수저수지 주변 호랑가시나무개체군을 식물사회학적 방법으로 분류하고, 식생과 토양과의 상관관계를 밝히고자 분포서열법에 의한 분석을 실시하였다. 호랑가시나무개체군은 꾸지뽕나무우점개체군, 상동나무우점개체군, 예덕나무우점개체군으로 분류되었다. 토양분석 결과 유기물함량 14.62~17.35%, 전질소함량 0.39~0.51%, 유효인산함량 8.83~20.15mg/kg, 치환성 K 0.44~0.64cmol+/kg, Ca 5.79~6.87cmol+/kg, Mg 3.43~4.19cmol+/kg이며, 토양 pH는 5.41~5.80인 것으로 조사되었다. 예덕나무우점개체군은 유효인산과 유기물함량이 많고 치환성 K, Mg의 양료가 적은 입지에 분포하는 것으로 분석되었다. 상동나무우점개체군과 꾸지뽕나무우점개체군은 유효인산과 유기물함량이 적고 치환성 K, Mg의 양료가 많은 입지에 분포하는 것으로 조사되었다. 호랑가시나무 자생지 보호를 위해 수관층을 피압하고 있는 덩굴식물 제거작업이 이루어져야 하며, 지속적인 생태모니터링과 자생지 보존을 위한 관리방안이 수립되어야 한다.

Genetic variation within and among 12 populations of whip grass in south China were investigated using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP). High genetic diversity was found in whipgrass by two molecular makers (PPB=86.21%, I=0.357 based on ISSR; PPB=82.21%, I=0.352 based on SRAP). However, there was relatively low genetic diversity at population levels. A high degree of genetic differentiation among populations was detected based on different measures and different molecular markers. We also found that SRAP markers were more efficient than ISSR markers in this species. Based on these findings, sampling strategy was proposed for successfully utilizing the genetic resource of this species.

본 연구는 군산 월명공원내 청사조 자생지의 식생구조 및 청사조 개체군 동태를 파악하여 청사조 생태 및 자생지 보존계획에 기초자료를 제공할 목적으로 수행되었다. 청사조 자생지에 대한 조사연구와 분석한 결과는 다음과 같다. 청사조 자생지의 입지로는 해발고 81~93m의 급경사지 해안사면으로 토성은 미사질양토였고, 토양pH는 4.1~5범위로 비교적 강산성을 띠었다. 조사구내 관속식물은 33과 51속 54종 6변종 1품종으로 총 61종이 확인되었는데, 이중 목본식물은 37종(60.7%), 초본식물은 24종(39.3%)이었다. 이중 청사조가 식물구계학적 특정식물종 V 등급으로 평가되었다. 청사조는 7 군데에서 군락으로 출현하였는데 월명산에서는 졸참나무, 사방오리나무, 굴피나무, 아까시나무 군락의 하층부인 관목층에서 중요치가 각 30, 15, 27, 65%로, 지피층에서는 중요치가 각 12, 27, 20, 18%로 주로 우점하며 출현하였고, 장계산에서는 3군데의 소나무 군락 하층부인 관목층에서는 중요치가 각 18, 45, 35%로, 지피층에서는 중요치가 각 11, 18, 21%로 우점하면서 나타났다. 청사조와 더불어 항수반종으로서는 국수나무와 쥐똥나무였다. 청사조의 개체군에 대한 전수조사에서 총 103개체가 확인되었고, 개체군의 공간분포에 따른 출현 형태는 전형적인 집중분포 형태를 띠고 있었다. 청사조 개체들의 평균 수고는 133cm, 평균 근원경은 4.4cm, 평균 가지의 분지수는 9.4개로 나타났다. 청사조 자생지의 보존 및 관리방안으로는 현 자생지의 지속적인 모니터링과 더불어 청사조의 꽃, 열매, 번식에 대한 기초연구가 선행되어야 하며, 자생군락지의 보호를 위해 휀스 등의 보호시설을 설치하고, 자생지내 경쟁수종의 제거 등을 제시하였다.

금오산과 가야산 지역에 분포하는 대마참나물 개체군이 발견되었으며, 이 개체군의 생태학적 특성을 밝히기 위하여 식생구조와 토양을 분석하였다. 대마참나물 개체군은 대마참나물 전형 우점개체군과 자주꿩의다리 우점개체군으로 분류되었다. 대마참나물 개체군 분포 지역의 유기물 함량은 25.0~32.3%, 전질소 함량은 0.83~1.04%, 유효인산 함량은 5.58~20.76ppm, 양이온치환용량(CEC)은 46.3~62.9이고, 토양 PH는 4.5~5.1로 나타났다. 대마참나물 개체군과 환경 요인들과의 상관관계를 보면, 전형 우점개체군은 양료 중 치환성 K, Ca, Mg와 양이온치환용량이 다소 많고 해발고가 비교적 낮은 입지에 분포하고 있으며, 자주꿩의다리 우점개체군은 전형우점개체군보다 해발고가 다소 높고, 양료 중 치환성 K, Ca, Mg와 양이온치환용량이 다소 적은 입지에 분포하고 있었다.

월출산국립공원 전 지역을 대상으로 146개의 조사구를 설치하여 상록활엽수 개체군 동태를조사한 결과, 월출산국립공원내 자생하는 것으로 확인한 상록활엽수종은 총 13종이었고, 주요 곡간부를 중심으로 최대 해발 450m까지 분포하는 것으로 조사되었다. 월출산국립공원에 자생하는 상록활엽수중 동백나무, 사스레피나무, 붉가시나무는 식물군락을 이루며 분포하였으나, 참식나무, 모새나무, 차나무, 보리밥나무, 광나무, 자금우는 반상이나 단독 형태로 분포하였다. 지피식물인 마삭줄, 백화등, 송악은 국지적으로 희소하게 출현하였다. 월출산국립공원의 일부 지역에서 소규모 형태로 붉가시나무림이 분포하였고, 상록활엽수들은 소나무군락, 굴참나무군락, 상수리나무군락의 임상층에서 주로 분포하고 있었다. 붉가시나무군락은 그 세력이 확장될 것으로 생각되며, 붉가시나무가 생육하고 있는 소나무군락과 낙엽활엽수림군락들은 붉가시나무군락으로 식생천이가 예상된다.

1991~1994년에 수원에서 땅강아지 연령 분포의 계절적 변화와 산란수를 조사하였다. 새로 부화한 약충은 주로 9~10월에 우화하므로써 1년에 1회 경과하고, 일부가 약충으로 월도하여 8월경 우화하므로써 2년에 1회 경과하는 것으로 보였다. 산란실당 란수는 야외 월동 성충을 실내에서 산란시켯을 때 평균 36.002.84립, 양외채집 산란실에서 47.684.35립이었다. 자충당 산란실수는 평균 1.170.19개로 0~3의 범위를 보였다.

In crop breeding program, information about genetic dissimilarity on breeding resources is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations and inbred breeding. This study aimed to evaluate the genetic variation in Kenyan sunflower breeding lines based on simple sequence repeat (SSR). A total of 83 alleles were detected at 32 SSR loci. The allele number per locus ranged from 2 to 7 with an average of 2.7 alleles per locus detected from the 24 sunflower accessions and the average value of polymorphic information contents (PIC) were 0.384. A cluster analysis based on the genetic similarity coefficients was conducted and the 24 sunflower breeding resources were classified into three groups. The principal coordinates (PCoA) revealed 34% and 13.38% respectively, and 47.38% of total variation. It was found that the genetic diversity within the Kenyan sunflower breeding resources was narrower than that in other sunflower germplasm resources, suggesting the importance and feasibility of introducing elite genotypes from different origins for selection of breeding lines with broader genetic base in Kenyan sunflower breeding program.

We collected 32 maize inbred lines from eastern cereal and oilseed research center in Canada to develop new maize varieties. We also evaluated genetic diversity, genetic relationships, and population structure using 35 SSR markers. A total of 269 alleles were revealed in 35 loci with an average of 7.69 and a range between 3 and 15 alleles per locus. The genetic diversity values varied from 0.176 to 0.889 with an average of 0.691. The polymorphic information content varied from 0.171 to 0.879 with an average of 0.659. Population structure analysis indicated that 32 Canadian maize inbred lines comprised four major groups and one admixed group based on a membership probability threshold of 0.80. The four major groups contained 13, 2, 5 and 2 maize inbred lines, respectively. From genetic relationships analysis, the all inbred lines were divided into three main groups at 26% genetic similarity. Group I included 22 inbred lines, and Group II included 9 inbred lines. Group III consist of only one inbred line. The results in this study would be useful for the improvement and development of new cultivars, planning crosses for hybrids or development of inbred line in maize breeding program

Background : Codonopsis lanceolata is a perennial plant of Campanulaceae with characteristic flavor and aroma and this plant has saponin, flavonoid, and inulin, which are reported to have physiological activity and antioxidant activity. In contrast, breeding or study of C. lanceolata varieties had not been done for a long time. Genetic polymorphism and phylogenetic relationship analysis of the plants by region of the crops can help the collection of genetic backgroud data for variety development. Methods and Results : In this study, we collected 26 C. lanceolata lines (95 individual plants) from 26 regions in Korea. We genotyped the collected lines using SSR markers developed in the previous study and analyzed the population structure based on the results. Population structures were analyzed using model-based STRUCTURE software (version 2.3.4) using the following parameters: Number of clusters (K) set = 1 to 12; Number of Iterations = 5; Length of Burning Period = 100,000; Number of MCMC (Markov Chain Monte Carlo) Reps after Burnin = 100,000. As a result, Of the 26 collections, were genetically grouped into 6 or 7 groups. Conclusion : The 26 C. lanceolata collections (95 individual plants) were genetically grouped but not grouped by collected regions. These results suggest that C. lanceolata has diverse genetic backgrounds and this data could be used as a basis for genetic polymorphism analysis of Codonopsis species.

Understanding the genetic variation among landrace collections is important for crop improvement and utilization of valuable genetic resources. The present study was carried out to analyse the genetic diversity and associated population structure of 621 foxtail millet accessions of Korean landraces using 22 EST-SSR markers. A total of 121 alleles were detected from all accessions with an average of 5.5 alleles per microsatellite locus. The average values of gene diversity, polymorphism information content, and expected heterozygosity were 0.518, 0.594, and 0.034, respectively. Following the unweighted neighbor-joining method with arithmetic mean based clustering using binary data of polymorphic markers, the genotypes were grouped into 3 clusters, and population structure analysis also separated into 3 populations. Principal coordinate analysis (PCoA) explained a variation of 13.88% and 10.99% by first and second coordinates, respectively. However, in PCoA analysis, clear population-level clusters could not be found. This pattern of distribution might be the result of gene flow via germplasm exchanges in nearby regions. The results indicate that these Korean landraces of foxtail millet exhibit a moderate level of diversity. This study demonstrated that molecular marker strategies could contribute to a better understanding of the genetic structure in foxtail millet germplasm, and provides potentially useful information for developing conservation and breeding strategies.

Assessing genetic diversity, population structure, and linkage disequilibrium is important in identifying potential parental lines for breeding programs. In this study, we assessed the genetic and phenotypic variation of 174 normal maize (Zea mays) inbred lines and made association analyses with respect to nine agronomical traits, using 150 simple sequence repeats (SSR). From population structure analysis, the lines were divided into three groups. Association analysis was done with a mixed linear model and a general linear model. Twenty one marker-trait associations involving 19 SSR markers were observed using the mixed model, with a significance level of P<0.01. All of these associations, as well as 120 additional marker-trait associations involving 77 SSR markers, were observed with the general model. Two significant marker-trait associations (SMTAs) were detected at P ≤ 0.0001. In the mixed linear model, one locus was associated with water content, two loci were associated with 100-kernel weight, setted ear length, ear thickness and stem thickness; three loci were associated with ear height, four loci were associated with total kernel weight and five loci were associated with plant height. These results should prove useful to breeders in the selection of parental lines and markers.

Soybean (Glycine max L.) is crucial legume crop as source of high quality vegetable protein and oil, and Korea is regarded as a part of center of soybean origin. To expand the information of conserved genetic diversity, we analyzed the genetic variability of soybean collection mainly introduced Korean accessions using 75 microsatellite markers. A total of 1,503 alleles with an average value of 20.0 alleles were detected among 644 accessions. Korean collection revealed average allele number of 13.4 while Chinese, Japanese and Southeast Asian accessions showed 9.0, 5.4 and 6.5 mean alleles, respectively. Especially, Korean accessions showed more number of private allele per locus as 3.4 contrary to other geographical groups. The mean expected heterozygosity and polymorphic information content was 0.654 and 0.616, respectively, and expected heterozygosity values were not significantly distinguished according to the geographical groups. The phylogenetic dendrogram and deduced population structure based on DNA profiles of 75 SSR loci showed Korean accessions formed distinct gene pool against Chinese accessions, and could be divided into five subpopulations. Korean soybean accessions have specific genetic diversity and might be serve the valuable alleles for bio-industry as a part of the center of soybean origin.