Background: The cumulus cells (CC) play an essential role in protecting oocytes and providing molecular signals for meiotic and cytoplasmic maturation. Gonadotropins stimulate CC proliferation, promote the release of factors that resume oocyte maturation, and activate small G proteins. Among these, Ras, a GTP-binding protein, participates in signaling pathways that regulate cell growth, division, and proliferation. This study aimed to investigate the changes in the Ras subfamily and gonadotropin receptor expression during porcine CC maturation. Methods: Cumulus-oocyte complexes (COCs) were incubated in a medium supplemented with follicular stimulating hormone (FSH), luteinizing hormone (LH), and epidermal growth factor (EGF) for 44 hours. CCs were collected from the COCs at 0, 22, and 44 hours, and mRNA expression levels of gonadotropin and growth factor receptors (FSHR, LHR, EGFR), Ras subfamily members (H-Ras, K-Ras, N-Ras, R-Ras), and Ras GTPases (RASA1, SOS1) were analyzed using quantitative RT-PCR. Results: The results revealed that LHR and R-Ras mRNA expression significantly increased only at 44 hours compared to the 0-hour group (p < 0.05). Conversely, RASA1 mRNA levels decreased significantly at the same time points. No significant changes were observed in H-Ras, K-Ras, N-Ras , or SOS1 expression. Conclusions: In conclusion, the observed increase in LHR and R-Ras and the decrease in RASA1 provide new insights into the molecular dynamics of Ras subfamily members during porcine CC maturation, contributing to a better understanding of the regulatory mechanisms underlying oocyte development.

Cell adhesion plays an important role in the differentiation of the morphogenesis and the trophectoderm epithelium of the blastocyst. In the porcine embryo, CDH1 mediated adhesion initiates at compaction before blastocyst formation, regulated post-translationally via protein kinase C and other signaling molecules. Here we focus on muscle RAS oncogene homolog (M-RAS), which is the closest relative to the RAS related proteins and shares most regulatory and effector interactions. To characterize the effects of M-RAS on embryo compaction, we used gain- and loss-of-function strategies in porcine embryos, in which M-RAS gene structure and protein sequence are conserved. We showed that knockdown of M-RAS in zygotes reduced embryo development abilities and CDH1 expression. Moreover, the phosphorylation of ERK was also decreased in M-RAS KD embryos. Overexpression of M-RAS allows M-RAS KD embryos to rescue the embryo compaction and blastocyst formation. Collectively, these results highlight novel conserved and multiple effects of M-RAS during porcine embryo development.

본 연구는 지리산 국립공원 내 유역면적 25km2인 달궁 유역의 홍수범람 및 침수 모의를 위하여 수행하였다. 수문모형으로 HEC-HMS를 이용하여 강우-유출해석에 의한 홍수량을 추정하였으며, 달궁 유역 침수 모의를 위한 수리모형으로는 HEC-RAS를 선정하였다. HEC- HMS를 이용하여 홍수량을 추정하고, 하천 단면 현지측량 자료를 HEC-RAS에 입력하여 지형자료를 생성한 뒤, HEC-RAS를 통해 입력된 지형자료와 HEC-HMS가 계산한 홍수량 자료와 현지에서 계측된 수위 및 유량자료를 이용하여 수면곡선과 유속 등을 계산하여, 강우사상에 따라 침수 모의를 수행하였다. 침수 모의 결과, 국립공원관리공단에서 제시하고 있는 재해요인 기준과 같이 시간당 70.0∼80.0mm 이상의 집중호우 시에는 침수 및 범람의 우려가 있을 것으로 판단된다. 한편, 침수 모의 결과로 얻은 최고수위점 자료를 바탕으로 ArcView를 이용하여 침수지도를 작성하였다. 본 연구를 통해 얻어진 국립공원의 산지하천 유역에 대한 침수 모의 결과는 국립공원의 침수재해지도의 작성 및 침수재해에 대한 관리 대책 수립에 활용될 수 있을 것으로 판단된다.

Several tumor animal models have been provided as a tool for developing cancer therapy. Here, we developed rapid, easy-to use, and cost-effective new rat animal model for invasion and metastasis of cancer using genetically k-ras-induced rat kidney cells(RK3E-ras). We observed tumor as early as 3 days after injection of RK3E-ras cells in subcutaneous of Sprague-Dawley rats. Tumor size and volume were increased exponentially for 2 weeks. The tail vein injected rats obtained the lethal infiltration in the lung within 2 weeks. This tumor animal model has great potential for studying cancer processes and short-term screening of variable cancer therapy strategy.

The principles to attain improved accuracy in a new controller for large CCD and mosaics detectors with the application of 16- and 32-bit DSP are presented.

In recent localized heavy rainfalls have been arising from abnormal climate change. People are concerning about damages with increasing the frequency of flooding. Therefore, we need to understand river hydraulic characteristics and management to reduce damage from flooding. To study hydraulic characterization of Sincheon experimental catchment HEC-RAS (Hydrologic Engineering Center River Analysis System) model which provided by U.S Army Corps of Engineers (USACE) was applied. This study analyzed and compared water level the frequency flood for 100 years and 200 years by clark unit Hydrography. The change of the water level of Daejeon bridge, Sincheon bridge and Singi bridge showed increased for all conditions. The flow rate for the Daejeon bridge and the Sincheon bridge showed an increase, but the Sinki bridge showed a decreasing flow rate overally, except for 1hour-100 years. The verification result showed that the model was able to simulate the water level with 0.4709 coefficient of determination and error ration ranging from 1 to 3%.

하천에 설치되는 강변저류지의 효율적 설계를 위해서는 정확한 부정류 흐름 해석이 반드시 필요하다. 일반적으로 하천의 부정류 수치모의는 1차원 부정류 수치모형인 HEC-RAS를 많이 사용하고 있다. 하지만 강변저류지가 있는 하도에서의 부정류 흐름에 대한 수리실험과 현장 모니터링 자료가 거의 없기 때문에 HEC-RAS를 이용한 부정류 수치모의 결과의 신뢰도를 확신하기가 어렵다. 따라서 본 연구에서는 부정류 수리실험을 수행하여 강변저류지가 있는 하도에서의 HEC-RAS를 이용한 부정류 수치모의 적용성을 검토하였다. HEC-RAS 적용성 검토는 직선수로에 강변저류지가 설치되어 있거나 그렇지 않은 경우에 대해 부정류 수리실험을 실시하여 측정된 결과와 비교하여 평가하였다. 특히, 강변저류지가 설치되어 있는 경우는 강변저류지의 저류용량이 충분하여 하도에서 강변저류지로 통하는 위어에 완전 횡월류 흐름만 발생하는 경우에 대해 실험을 수행하였다. 강변저류지가 설치되지 않은 직선수로에 대한 HEC-RAS의 수위 계산 결과는 최대 3% 오차를 유량은 최대 1% 오차를 보였으며, 강변저류지가 설치된 경우의 HEC-RAS 수위 계산 결과는 최대 4% 오차를 유량은 최대 2% 오차를 나타냈다.