The purpose of this study was to evaluate the role of survivin in various salivary gland tumors. For this study, total 18 cases of salivary gland tumors; 6 cases of benign and 12 cases of malignant tumors were used as experimental group. In benign tumors; pleomorphic adenoma, oncocytoma, and in malignant tumors; adenoid cystic carcinoma, mucoepidermoid tumor, high grade and low grade malignancy each, adenocarcinoma, acinic cell adenocarcinoma cases were included. And for the control group, fresh submandibular glands were attained from gnathosurgical specimen. All the specimens, experimental, control group were fixed in 10% neutral formalin solutions, embedded in paraffin, sectioned 5um or more in thickness, stained with the hematoxylin and eosin, mounted and examined under the microscope. For the immunohistochemical studies, all the specimens were activated with survivin monoclonal, and secondary antibodies as usual manners, and taken photos on various pathologic fields analysed with the image analysis system, and evaluated the positive and negative stained area in the tumors on each images and statistically analyzed with SPSS 15.0 program. Attained result as follows. In control group, in part, acini cells show positive reaction on the nuclei, negative on the all most of the cytoplasm, more intense reaction on the cytoplasm and nuclei on the serous demilune (47.33%). In experimental group, all the specimens show survivin positive reaction on the cytoplasm with/or without positive reaction on nuclei according to the tumors, in benign tumors; pleomorphic adenoma (63.48%), oncocytoma (56.31%), each and in malignant tumors; adenoid cystic carcinoma (87.6%), acinic cell adenocarcinoma (56.35%). adenocarcinoma (67.47%), mucoepidermoid carcinoma, low grade (70.76%). high grade (55.23%). Survivin expression shows higher in tumors compare to that on the control group (p<0.05), but between the malignant tumors no significant are not noted(p>0.005). Survivin expression is strongly related to the malignancy of salivary gland tumors

The purpose of this study was to evaJ uate the role of integrin a 3 and integrin ß 1 expression in the saivary gJand tumors. For this study, 11 specimens diagnosed as pleomorpic adenoma, adenoid cystic carcinoma, adenocarcinoma, mucoe pidermoid carcimoma referred to the Dept. of Oral Pathology‘ School of Dentistry, Kyung Hee University, 2 specimens 01' normaJ submandibular gland tissues were used as experimental, control groups respectively, All the tissues experimental and control group wel'e fixed in neutral formaJin solution and embedded in paraffin, seriaJ tissue section were made 511m in thickness and processed in the standard way for immunohistochemical method, using primary antibody against integrin a 3, and integrin ß 1 each was diluted at 1;100 followed by the poly- horse radish peroxidase detection system with DAB as chormogen counterstained with Mayel ’s hematoxylin stain method and mounted And examined unde1' the biologic micro scope with the criteria of no epitheliaJ stain, weak 01' focal epithelial stain, moderate 01' focal intensive epithelial s tain. intense generalized epithelial staining for the epithelial, and connective tissue components in no1'mal salivary gland, and saivary g land tumors : pleomorphic adenoma‘ adenoid cystic carcinoma, adenoca1'cinoma, mucoepide1'moid ca1'cinoma on each On the integ1'in α 3 reaction, negative to minimal posit ive reaction was noted on the salivary gland twnors and nor mal subma ndibular gland tlssues On the integrin ß 1 reactions, intense 1'eaction is shown on the serous demilune and ductal cells , and partly on the serous acini in submandibula1' gland tlssues On the integrin ß 1 reactions to pleomorphic adenoma tissues, moderate reactions were noted on the ductal celJs and myoepithelial cells. On the integrin ß 1 reactions to adenoid cystic ca rci noma‘ adenocarcinoma, mucoepidermoid ca1'cinoma tissues, intense reactions were shown on the neo plastic cell s , This resuJt suggest that integrin a 3. integrin ß 1 could be a 1'ole inducing the tumorigenesis.

1'he purpose 0 1' this study was to evaluate the ro1e of c• fos and c-jun expression in the salivary gland tumol‘ s , For this study‘ 11 s ubj ects of sali vary gland tumors ; 4 su이 ects of p1eomorphic adenomas, 3 s ubj ects of adenoid cystic car cinomas , 2 s ubjects of adenocar cinomas, 2 subjects of mucoepidermoid tumors, referred to the Dept, of Ora l Path College of Dentis t l'Y, Kyung Hee Univer sity, were used as experimental group, and 2 subj ect s of normal minor salivary gla nds without a ny infla mmator y changes, were used as control group respectively, All the tissues experimenta l and control group were fixed in 10% neutral formalin solution and embedded in paraffin , serial ti ssue section were made 5 I1I1l in thickness a nd processed in the s t andard way for immunohistochemical method, using primary and secondar y a ntibodi es, for c-fos, c-jun, foll owed by the Streptavidin-Horse Radish Peroxidase, all BioGenex U,S,A, made, appli cat ion counter s t ained with Mayer's hematoxylin stain method, mounted And examined under the biologic mi croscope, with the criteria : -(no epitheli al s ta in), +(weak or focal epithelial stain), ++(moder a te or focal intensive epithelial sta in)‘ +++(intense generali zed epithelial staining) for the epithelial, and stromal ti ssues on each Attained results as follows : 1 1n nonna l minor saliva ry gla nds , it is noted that negative responses on the acini minimal res ponses in nucl ei and cytoplams of se rous demilun e, myoepi theli al cells, and intensive r esponses in nuclei and cytoplam of ducta l cells to c-fos and c-jun, 2 1n the res ponses to c-fos, positive responses in nuclei and cytoplasms of the lining cells o[ the ad e nαd tissues, e pidermoid, and mucous cells in mucoepidermoid tumors are noted and in other tumor tissuses, negative nuclei wi th pos itive cyto plasms are revealed 3 1n the responses to c- jun, it is noted that positive r es ponse in nuclei and cytolasm i n the cells of adenoid tissues in pl eomorphic adenoma, epidermoid cell s, mucous cell in mucoe pi dermoid tumor but in other tumor s, only positive responses in cytoplasm are noted, Intensive r esponses on c-fos‘ c-jun were noted on the high a typical cells 1'his results suggest that c-fos and c-jun may be affected t o the reactivation on growt h and development of the salivary gland tumors