This study compared the antioxidant and mineral properties of the leaves and seeds of fifteen Korean adzuki bean (Vigna angularis L.) breeding lines. This study was conducted in an attempt to expand the use of Korean adzuki bean leaves. The potassium, calcium, magnesium, and sodium contents of the leaves were significantly higher than the seeds, in particularly, the potassium content. The leaves had approximately 3.3 times higher potassium content than the seeds. For instance, the potassium content of YA1317 leaves was 21% higher than that of Arari. The total polyphenol content and ABTS activity of Adzuki bean leaves were significantly higher than the seeds, as opposed to the total flavonoid content and DPPH scavenging activity. Among the 15 breeding lines, YA1402 had 1.2~3.2 times higher antioxidant content and activity as compared to the Arari variety. It was concluded that adzuki bean leaves had higher mineral content, antioxidant component and activity as compared to the seeds. Therefore, adzuki bean leaves could be used an ingredient for dishes and as a medicine.

Females of adzuki bean beetle (ABB), Callosobruchus chinensis (L.), a cosmopolitan field-to-storage pest of legumes, release sex pheromone consisting of two isomeric components, 2Z-homofarnesal and 2E-homofarnesal. Two day old virgin ABB adults were exposed to sub-lethal fumigation doses (LC25=0.0056 and LC50=0.0076 mgL-1) of phosphine (PH3) to evaluate the effects on adult longevity, fertility and sex pheromone production. Longevity (female; P < 0.001, male; P < 0.001), fecundity (P < 0.001) and sterility (P < 0.001) were significantly affected. The GC-MS for solid phase micro extraction exhibited the significant reduction of release of both of the pheromone components; 2Z-homofarnesal (P < 0.0038) and 2E-homofarnesal (P = 0.0009) when the females were exposed to LC25 dose of PH3. This study unveils the detrimental effects of PH3 fumigation on some of the biological parameters of ABB.

Appropriate water level is the primary factor for the optimal yield of crop plants. The required water level varies according to the variety of the crops. In the present study, we investigated the optimum requirement of groundwater level(GWL) to grow sorghum and adzuki bean under paddy field soil. Here, we cultivated sorghum and adzuki bean using lysimeter filled with paddy soil under GWL 0 cm(NT) and GWL(20, 40 cm) where GWL 20 cm is maintained as a waterlogging condition. The plant growth promoting attributes were measured on the first day after treatment(0 DAT), 10 DAT and 20 DAT. The results showed that the growth parameter such as shoot length, leaf length, leaf width, and stem thickness of both sorghum and adzuki bean were constantly increased and were found higher at GWL 40 cm(except stem thickness and leaf width in sorghum at 20 DAT). The physiological parameters such as chlorophyll content and stomatal conductance were also found higher at GWL 40 cm in all DAT. In addition, the elements like P and K contents in adzuki bean, and Ca content in sorghum were constantly increased and was found higher in GWL 40 cm at all DAT. These results suggest that the GWL of 40 cm is appropriate for production of sorghum and adzuki bean especially in case of paddy soil.

Azuki bean beetle (ABB), Callosobruchus chinensis (L.) is a cosmopolitan field-to-storage pest of legumes. Its female sex pheromone consists of two isomeric components, 2Z-homofarnesal and 2E-homofarnesal. Two day old virgin adults were exposed to sub-lethal fumigation doses (LC25=2.80 and LC50=3.32 mgL-1) of ethyl formate (EF) to see the effects on adult longevity, fecundity, sterility and female sex pheromone production. Longevity (female; P < 0.001, male; P < 0.001), fecundity (P < 0.001) and sterility (P < 0.001) were significantly affected. The Gas Chromatography-Mass Spectrometry for solid phase micro extraction revealed that release of both of the pheromone components were not affected by LC25 dose of EF. However, the fecundity (per female/day) was significantly (P < 0.001) stimulated by the tested EF doses when the female adults were treated irrespective of the male treatment, probably revealing a hormetic effect. This study provides insight into the effect of EF fumigation on biological activities of C. chinensis.

We investigated the influence of germination and high hydrostatic pressure (HHP) treatment conditions on the conversion of functional compounds and antioxidant activity in adzuki bean. The adzuki bean germinated at 25°C for three- or six-days, and was later subjected to HHP at 0.1, 50, 100, or 150 MPa for 24 h. The highest polyphenol content (5.36 mg gallic acid equivalents (GAE)/g) and flavonoid content (0.91 mg catechin equivalents (CE)/g) were observed after germination for six days and HHP treatment at 100 MPa for 24 h, respectively. The total phenolic acid contents increased with increasing applied pressure from 88.86 to 208.26 μg/g (100MPa, 24h). Phenolic acids are divided into two categories; those that exhibit increased content upon HHP treatment, and those that exhibit decreased content. The increasing phenolic acids were gallic acid, chlorogenic acid, (+)-catechin, ρ-coumaric acid, ferulic acid, heperidin, salicylic acid, protocatechuic acid, cinnamic acid, naringenin. The total anthocyanin content decreased with increasing applied pressure from 22.42 mg/100 g to 6.28 mg/100 g (150 MPa, 24 h). The highest ABTS radical scavenging activity (8.02 mg eq AA/g) and DPPH radical scavenging activity (1.22 eq Trolox/g) were observed after germination for six days and HHP treatment at 100MPa for 24h, respectively. These results suggested that the combination of HHP and germination can lead to improved functionality in adzuki bean.

Although expression of foreign genes in a crop species has been target techniques for powerful protection against insect pests, classical breeding programs using varietal resistance of a crop are still being processed. It is, however, frequently difficult to find key products expressed from resistance-related genes in the variety, and those action mechanism. Here, an unterminated story about seed of a mungbean (Vigna radiata) variety with high resistance against the adzuki bean weevil and the bean bug is introduced. The two insects cannot survive on seeds of the variety, although they well develop on susceptible ones. Molecular markers linked to the resistance were selected through BAC library screening and near isogenic lines, and finally a bruchid resistance gene was suggested after map-based cloning approaches. Starvation and chemicals were suggested for a resistant mechanism and a related factor through feeding and behavior experiments, respectively. The seed flour was extracted with organic solvents, and isolated into several fractions on chromatography. Several peaks on HPLC in a fraction were related with the high mortality of the bean bug. A partial structure in an isolated chemical was observed before full identification.

1. Pepsin과 pancreatin 소화물들을 비교한 결과, 순수한 팥 단백질 중에서 소화효소 저항성을 가지는 단백질이 존재하는 것으로 확인되었다. 2. 팥의 주요 단백질을 제거하고 pepsin과 pancreatin으로 소화시켰을 때 더 많은 분해가 일어나는 것으로 보아 팥의 주요단백질들 중에 소화효소 저항성을 가지는 것이 많을 것이라 추측된다. 3. 팥의 주요 단백질들은 장 점막세포와는 크게 작용하지 않는 것을 확인할 수 있었다. 4. 팥 단백질의 데이터베이스 구축과 팥 단백질이 다른 영양소들과의 상호작용을 하는 지에 대한 연구가 진행되어야 할 것이다.

This study was conducted to determine the oviposition preference and development of adzuki bean weevil, Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) on five different leguminous seeds a) cowpea, b) white kidney bean c) soybean cultivar Daepung d) mung bean and e) azuki bean. A multiple choice test and a nochoice test were conducted to record the number of eggs laid, total developmental time, number of adults emerged, and adult longevity of C. chinensis on the five different leguminous seeds. Both choice and no-choice tests revealed cowpea seed as the most preferred oviposition substrate. Total developmental time from egg to adult stage ranged from 28 to 31 days; shortest of that on the cowpea followed by adzuki and mung bean seeds. Successful development of C. chinensis was not witnessed on soybean or on kidney bean. Rate of adult emergence was higher on adzuki and mung bean seeds with longer adult longevity of those emerged from adzuki bean and cowpea. Higher egg laying preference and shorter developmental time with longer adult longevity of the beetle on cowpea probably signifies importance of physical as well as chemical attributes of the seed as an oviposition substrate other than evolutionary history of the insect. Cowpea seeds can possibly be used as a pull stimulus either as a trap crop or as a stimulant in the mechanical traps against C. chinensis where other leguminous host crops are grown as main crops.

본 연구는 농업유전자원센터에 보존되어있는 우리나라 원산재래종 팥 유전자원 164점을 대상으로 농업기초형질을 조사하고, 팥 근연종을 포함하여 180점을 대상으로 분자마커를 이용하여 우리나라 재래종 팥 유전자원의 유전적 다양성을 분석한결과는 다음과 같다. 1. 기초 특성조사 된 자원의 개화일수는 최소 44일에서 최대 83일, 평균 67.1일이었으며 성숙일수는 최소 27일, 최대 42일, 평균 34.2일 이었고 생육일수는 최소 83일, 최대 118일, 평균 101.2이었다. 조사된 자원들은 북한원산자원들이 남한원산자원들보다 다소 일찍 개화하나 늦게성숙하는 경향을 보였다. 2. 대비품종인 충주팥의 개화일수는 65일, 성숙일수는 42일, 생육일수는 107일로 대비품종에 비해 우리 재래종 팥 자원의 평균개화일수는 약 2일 늦은 반면 성숙일수는 약 8일정도 빨랐고, 생육일수는 약 6일정도 빨라 평가 대상재래종자원은 대비품종인 충주팥에 비해 조숙종이 많은것으로 조사되었다. 3. 본 시험에 이용된 우리나라 재래종 자원은 일본의 기상생태형에 배치해본 결과 비교적 조생종에 속하는 것으로분석되었다. 4. 10개의 SSR 마커를 이용하여 다양성을 분석한 결과 PIC0.70을 얻었으며, 집단별로 분석한 결과 다양성은 야생종집단이 가장 다양성이 높고 남한원산 자원이 가장 낮은것으로 조사되었다.

Three lepidopteran insect pests of the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), the soybean pod worm, Matsumuraeses phaseoli (Lepidoptera: Tortricidae) and an Ostrinia spp. (Lepidoptera: Pyralidae) attacking adzuki bean, Vigna angularis, were confirmed as the major insect pests during the reproductive developmental stage of adzuki bean, and M. vitrata existed dominantly. Almost all plants in the adzuki bean field were in jured by at least one of the three species, and 15~60% of reproductive organs were injured. Unbloomed inflorescence occurred continuously through the reproductive stage of adzuki bean. While the flower was many at the early stage and its number soon decreased, the pod was few at the early stage, but its number increased soon and maintained at a constant level. The results suggested that the adzuki bean injured by insect pests compensate the flower loss by developing new inflorescence, but the compensation do not cause the development of new pods. While M. vitrata and M. phaseoli were observed in flowers, pods and stem mainly during the first half of reproductive stage of adzuki bean, Ostrinia spp. was observed only in pods and stem during the second half. In addition, while all instars of larvae of M. vitrata were observed, larvae between the third and fifth instars for M. pahseoli and Ostrinia spp. were observed.

The adzuki bean (Vigna angularis L.) is a red-grained legume that has a number of essential nutrients and is used in traditional dishes in Asia. Adzuki bean industrial by-products are also a potential low-cost source of some unique bioactive polyphenols. Hence, here, the authors aimed to perform a comparative study of the phytochemical profiles of the leaves and seeds of the adzuki bean and compare their antioxidant, α-glucosidase inhibition, and tyrosinase inhibition activity. The authors assessed antioxidant activity by DPPH, ABTS, FRAP, PR, TPC, and SOD assays, which showed wide variation, respectively. From the relative antioxidant capacity index results, 10 adzuki bean landraces were selected to compare for phytochemicals and bioactivity using leaf and seed extracts. Antioxidant, α-glucosidase inhibition, and tyrosinase inhibition activity in the leaf extracts were higher than in the seed extracts, and there were more flavonols and isoflavones in the leaf extracts than in the seed extracts. This study demonstrated that adzuki bean leaf extracts could be a new natural antioxidant or antidiabetic agent and a skin whitener and can also be used in industrial applications.

This study investigated the changes in yields, antioxidant compounds, and antioxidant activities among 11 adzuki bean cultivars cultivated in a paddy field with somewhat poorly drained soil. The adzuki bean cultivars were cultivated in the paddy field from 2015 to 2016 in Milyang, Gyeongsangnam, Korea. Average soil moisture content was 16.5% in 2015 and 31.0% in 2016 at the experimental site during adzuki bean cultivation. As the soil moisture content increased, most of the adzuki bean cultivars showed deceases in stem height, first setting pod node, number of pods, 100 seed weight, and yield. Chungju-pat produced the greatest yields among the 11 cultivars in 2015 and 2016, whereas Hongeon had the smallest yields. Adzuki bean yields after paddy field cultivation was lower for all cultivars than for adzuki bean yields from the same cultivars after upland field cultivation. Chungju-pat and Chilbo-pat showed the smallest decreases in yields after paddy field cultivation, whereas Hongeon and Kumsil showed the greatest yield reductions. There were no significant differences in proximate composition. Some mineral components (P2O5, Ca, and Mg) were statistically different across cultivars. Chungju-pat had the highest Ca and Mg contents, but antioxidant components (polyphenol and flavonoids) and antioxidant activities (ABTS and DPPH) were highest in Saegil and lowest in Jungbu-pat. PCA and clustering analyses, based on the growth, yield, and antioxidant component measurements, performed to identify which variables contributed the most to separating adzuki bean cultivars or to grouping cultivars with similar characteristics. These analyses showed that the antioxidant components and antioxidant activities had the most influence on grouping cultivars together. Among the 11 cultivars, Saegil was statistically different from the other cultivars, but the other 10 cultivars were not significantly different under paddy field cultivation. Soil moisture content affected adzuki bean yield and antioxidant component contents. An increase in soil moisture led to a decrease in yield, but an increase in antioxidant components. These results provide information that will improve the selection of an appropriate adzuki bean cultivar for use in paddy fields.

This study investigated the changes of antioxidant compounds and antioxidant activity of adzuki bean by drainage methods in poorly drained sloping paddy field. The soil moisture contents of under pipe and open ditch drainage on very poorly drained paddy soil were 18.52±4.58 and 19.01±4.25%, and imperfectly drained paddy soil were 14.87±4.82 and 18.64±3.85%, respectively. Moisture, protein, fat and ash contents of adzuki bean with drainage methods were 10.10~11.60, 14.13~21.75, 0.02~0.73 and 2.81~3.45 g/100 g, respectively. The total polyphenol, flavonoid and tannin contents, and radical scavenging activity of adzuki bean showed significant differences by drainage methods. The total polyphenol, flavonoid, and tannin contents by drainage methods were 2.73~4.14 mg GAE/g, 1.07~1.43 mg CE/g, and 1.27~1.84 mg TAE/g, respectively. The DPPH and ABTS radical scavenging activities were 2.84~4.47 and 5.11~6.74 mg TE/g, respectively. The antioxidant compounds and radical scavenging activity of the adzuki bean by drainage methods were frequently affected soil water.

국내 전역에서 수집된 팥 150 유전자원을 공시하여 작물학적 형질 특성조사를 통한 품종육성의 기초자료를 제공코자 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 신육형 빈도를 보면 무한신육형이 96.6%(145자원)를 차지하여 가장 많았으며, 유한신육형 및 반무한신육형은 각 각 2.0%(3자원), 1.3%(2자원)를 차지하였다. 2. 화색은 황색이 98.0%(147자원)를 차지하였으며, 협색은 노란색, 갈색, 농갈색, 흑황색, 혼합색의 5가지의 색으로 확인되었다. 이중 가장 많은 협색은 황색으로 64.3%(96자원)를 차지하였다. 종피색은 갈색, 농갈색, 흑색, 흰색, 혼합색, 적갈색, 황색 및 녹색으로 8가지의 립색을 나타내었으며, 적갈색 및 갈색계통이 전체의 66.5%를 차지하였다. 3. 생육일수는 89.0~142.0일의 분포를 보여 변이의 폭이 상당히 컷으며, 평균은 115.0일이었다. 생육일수의 빈도는 131.0~140.0일로 이에 속하는 자원이 37.2%(56 자원)를 차지하였으며, 다음은 121~130일 사이가 27.3%(41자원)를 차지하였다. 4. 협장은 5.9~10.1 cm를 나타내어 변이 폭이 컷으며, 평균은 7.8 cm이었다. 협장은 7.1~8.0 cm 사이에 속하는 자원이 45.1%(68 자원)로 가장 많았으며, 다음은 8.1~9.0 cm에 속하는 자원이 32.2%(48 자원)를 차지하였다. 5. 개체당 평균 협수는 28.5협이었으며 150 유전자원의 협수분포를 보면 5.0~132.5협을 나타내어 변이의 폭이 컷다. 개체당 협수가 가장 많은 자원은 11.0~20.0협 사이로 30.8%(46 자원)를 나타내었으며, 다음은 21.0~30.0협에 속하는 자원이 28.6%(43자원)를 차지하였다. 6. 협당립수는 5.3~11.3립을 나타내어 변이의 폭이 컷으며, 평균은 8.2립이었다. 협당립수가 가장 많은 그룹은 7.1~8.0립으로 32.2%(48자원)를 차지하였으며, 다음은 8.1~9.0립 사이에 속하는 자원이 28.2%(42자원)를 차지하였다. 7. 100립중은 범위가 5.7~23.0 g으로 변이 폭이 컷으며, 평균은 12.9 g이었다. 100립중의 분포는 9.01~13.0 g사이에 속하는 자원이 전체의 43.4%(65 자원)로 가장 많았으며, 다음은 13.01~17.00 g이 28.5%(43 자원)를 차지하였다.