재래유자, 개량종 I(유자+탱자), 개량종 II(유자+유자) 세가 지 유자의 종류별, 부위별 이화학적인 특성과 유기산, 지방산 및 향기성분을 분석하였고, 총 페놀 화합물을 분석하는 실험 을 수행하였다. 중량은 재래종이, 껍질(혹은 껍질+씨)의 비율 은 개량종이 높았다. 단백질은 재래종보다 개량종Ⅰ의 껍질 에서 다소 높게 나타났고, 회분도 개량종의 껍질에서 다소 높 았다. Vit. C는 과즙의 경우 재래종에서 29.6 ㎎%로 껍질에서 는 개량종 I이 57.7 ㎎%로 다소 높았다. 유리당은 과당이 주 였는데, 개량종 I에서 가장 높은 함량을, 유기산은 구연산이 대부분을 차지하였는데, 개량종 II에서 가장 유기산 함량이 높았다. 유자의 지방산 조성은 리놀레인산이 가장 많았고, 과 피와 과즙에서 품종별 특징을 보이고 있었다. 향기성분은 dl-Limonene이 향기성분의 64~70%를 차지하는 물질로, gammaterpinene과 함께 개량종 II에서 유의하게 높았다. 유자에 함 유된 총 페놀 함량은 품종별로는 유의한 차이를 보이지 않았 으나, 과즙에 비해 과피에 2.5배 정도 많은 양이 들어 있었다.

This study was conducted to find out the variation in agronomic trait and chemical composition in the collected Perilla frutescens from China and Japan. From the results of growth investigation, the maximum number if branches was 26.7ea in Japan 134 line, followed by 25 nodes number in China 119 line. Among the different lines investigated, maximum number of panicle number (108.8) were observed in China 114 line. 1000 seed weight was maximum (4.12 g) in China 118 line. Flowering time of different collected lines varied significantly with average value of 175.5 days and the average line required for maturation of seedlings was 205.1 days. Plant height was the highest (248.9 ㎝) in China 107 line. Highest number of total picking leaves was 965ea, and the average picked period was 54 days. The major phenol compounds contained in Perilla frutescens showed wide variation for Syringic acid, Benzoic acid, Naringin, o-Coumaric acid, Myricetin, Naringenin and Hesperetin. Japan 139 line showed the highest level of total phenol contents (8254.0 ㎍/g, dry weight).

Prunus sargentii of Rosaceae familiy, has been reported to have radical scavenging activity and anti-inflammatory effect. On these facts, this research was conducted to evaluate pharmaceutical activities of the bark extracts P. sargentii. Free radical scavenging activities of fraction (Fr) -5~10 isolated from P. sargentii was higher than 80% respectively at 10ppm. The superoxide dismutase (SOD)-like activity of Fr-5, 9 were about 97, 84%, respectively at 1,000 ppm. Xanthine oxidase inhibitory effect of Fr-9, 10 were about 75, 78%, respectively at 1,000 ppm. The tyrosinase inhibitory effect related to skin-whitening was 72, 68% in Fr-2, 9 isolated from Prunus sargentii R. at 1,000 ppm. Hyaluronidase inhibition activity related to the anti-inflammation effect was 98% for Fr-8 at 500 ppm. Isolation of the methanol soluble fraction from P. sargentii yielded two major phenol compounds, (-)-epicatechin and taxifolin. The structure of the compound was certainly determined by chemical analyses, as well as NMR and Mass spectroscopy. The present study was carried out in a search for new cosmetic material from the bark from P. sargentii. and, (-)-epicatechin and taxifolin were isolated as active principles. So P. sargentii R. methanolic extracts may be used for the cosmetic material.

채소류 21종, 과일류 10종, 식용꽃 5종과 wine 2종(복분자주, 포도주)의 poly-phenol함량과 항 산화력을 Folin-Ciocalteu 방법과 DPPH 방법을 사용하여 분석하였다. Poly-phenol함량의 범위는 채소는 최저치가 515.12ppm(무)이고 최고치가 2,385.44ppm(깻잎)으로 평균이 1,117.67ppm고, 과일은 최저치가522.43ppm(사과)이고 최고치가 2,453.32ppm(머루)이었고, 식용 꽃은 최저치가 962.44ppm(흰소국) 최고치가 5,610.80ppm(붉은장미)이었다. 복분자주는 671.52ppm 이고 포도주는 1,200.00ppm 이었다. 항 산화력의 범위는 채소는 최저가 0.16%(호박)에서 최고가 59.97%(냉이)이고, 과일은 최소가 5.62ppm(배)에서 최고가 79.62%(머루)이었고, 식용 꽃은 최저가 4.70ppm(금어초)에서 최고가 86.53%(진달래)이고, 복분자주는 52.02%이며, 포도주는 78.67%였다. 모든 재료들 중 식용꽃이 poly-phenol량과 항 산화력이 가장 높았고, poly-phenol 함량과 항 산화력의 상관관계는 채소에서는 인정하기 어려웠으나, 과일, 식용 꽃에서는 높았다. 같은 식물이라도 색깔이 짙은 부분이 옅은 부분보다 poly-phenol 함량과 항 산화력이 높았다.

뽕나무 열매(오디)를 이용하여 천연항산화제를 개발할 목적으로 오디로부터 페놀성 물질을 추출하여 항산화 활성을 측정하였다. 페놀성 물질의 추출은 60 ethanol을 사용하여추출하는 것이 수율이 가장 좋았으며, rutin과 isoquercetrin의함량이 높았다. 추출된 오디 추출물의 ABTS radical decolorization과 antioxidant protection factor(PF)를 살펴 본 결과 ABTS는 80 추출물이 약 98의

The objective of this study was to determine the role of storing years with the variation of total phenol and individual phenolic compounds in soybean (Glycine max L.) seeds. The total phenol content varied from 0.36 to 0.42% over four years, with the highest value (0.42%) found at storage for two and three years. Among the nine soybean varieties examined, Daweonkong had the highest total average phenol content (0.58%). The total content of 11 phenolics varied from 730.0 to 1812.8 ~mu~textrmg~;~textrmg-1 over storage for four years, and the highest concentration (1812.8 ~mu~textrmg~;~textrmg-1 ) was found at storage for two years. Myeongjunamulkong (1465.4 ~mu~textrmg~;~textrmg-1 ) had the highest mean content among the nine soybean varieties. The total content of 11 phenolic compounds measured in this study occupied from 20.96 to 47.73% of the total phenol contents. The highest total phenol contents were in seeds with black coats (5279.4 ~mu~textrmg~;~textrmg-1 ), while the highest concentration of individual phenolic compounds were in seeds with green coats (1419.5 ~mu~textrmg~;~textrmg-1 ). Our study suggests that it may be feasible to improve soybean varieties with high functional substances such as phenolic compounds.