This study was carried out to produce stable evening primrose oil in water emulsion by using various emulsifier with HLB (8.6, 12, 16.7), concentration (0-45%) and emulsification methods such as high-speed emulsification (7,000 rpm, 2 min) and high-pressure homogenization (10,000 psi, 1 cycle). And then properties of evening primrose oil in water emulsion was evaluated with keeping at room temperature and 40oC during 28 days. Lower HLB 8.6 and high viscosity emulsifier added emulsions were not appropriate for high-pressure homogenization and were separated in a day. The optimum emulsification condition was HLB 12 and high-pressure homogenization (10,000 psi, 1 cycle) for evening primrose oil in water emulsion. These emulsions produced by optimum condition were not separated with the aqueous phase and the oil phase and they were nano-sized around 200 nm, higher zeta-potential (±mV), mono-polydispersed (<0.3), and less oxidized (<0.4) during 28 days.

세라마이드는 일반적으로 화장품 산업에서 피부의 보습 및 피부장벽강화에 도움을 주는 성분으로 많은 연구와 개발이 활발하게 이루어지고 있다. 이번 연구에서는 일반적인 합성 세라마이드가 아닌 달맞이꽃오일에서 얻은 천연 세라마이드를 함유한 보습크림이 피부의 보습 및 경피수분손실량의 변화에 어떤 영향을 미치는지를 확인하였다. 세라마이드를 함유한 보습크림이 세라마이드들 함유하지 않은 보습크림과 비교하여 피부보습력과 경피수분손실량에서 우수한 결과 값을 나타내었음을 확인하였다.

Hemp seed oil and evening primrose oil were incorporated into the diets of laying hens for 5 weeks and the level of gamma fatty acid in the eggs that the treated hens laid was then evaluated. Hens were fed corn-soybean based diets that contained 5% tallow, 5% corn oil (CO), 5% hemp seed oil (HSO), or 5% evening primrose oil (EPO). The hemp seed oil and evening primrose oil influenced the amount of gamma linolenic acid found in the eggs through blood. The level of gamma linolenic acid in the plasma was significantly higher in hens that received the HSO and EPO diets than in those that received the tallow and CO diets. The HSO and EPO diets led to a 1.09% and 4.87% increase in egg gamma linolenic acids, respectively, when compared with eggs produced by hens treated with tallow and CO. Taken together, these data demonstrate that healthy eggs with increased gamma linolenic acids can be generated by minor diet modifications when hemp seed oil or evening primrose oil is included in the hen diet.

This study examined the effects of different levels of evening primrose oil(EPO) on the accumulation of γ-fatty acids in broiler meat. Six hundred one-day-old male chicks (Ross strain) from commercial broilers were divided randomly into 6 groups × 4 repeat pens. The broilers were fed experimental diets containing 4.0% tallow(control), 0.5% EPO, 0.7% mixed oil(EPO 70 : soy bean oil 30), 1.5% EPO, 3.0% EPO or 4.0% EPO for 35 days. There was a significant difference in body weight gain between the control and treatment groups except for the 0.5% EPO group (p<0.05). There was a significant difference in the percentage of thigh and breast weight against the carcass weight between control and treatment groups except for the 0.5% EPO group in the thigh and 0.5% EPO and 4.0% EPO groups in the breast weight (p<0.05). The saturated fatty acid levels of the skin and breast muscle lipid of the broilers fed diets containing EPO were significantly lower than that of the control group (p<0.05), while the level of unsaturated fatty acid was significantly higher than that of the control group (p<0.05). The γ-fatty acid(GLA, gamma-linolenic acid, 18:3n-6) level was particularly higher in the chicken meat lipids from the broilers fed EPO than in the control group (p< 0.05). This shows that feeding EPO to chicks can produce novel functional broiler meat that is enriched in gamma-linolenic acid.

The objective of this study was to determine the effect of dietary oils on the levels of the γ-linolenic acid in chicken meat lipids. Three hundred ten five, 1-d old, male, Ross strain, broiler chicks were fed for 35 d to compare diets containing evening primrose oil(EPO) and hemp seed oil(HO) to a control diet. Fatty acid composition of lipid from chicken skin, thigh and breast muscle were determined at the end of the trial. The level of γ-linolenic acid of lipids from chicken meat fed diets containing EPO or HO was significantly higher than that of the control group(p〈0.05). The level of γ-linolenic acid of lipids from chicken skin was highest in the group, which had been fed the EPO 0.85%, followed in order by EPO 0.7%, 0.5%, EPO mixed oil, HO and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken skin between the control and treatment groups(p〈0.05). The level of γ-linolenic acid of lipids from chicken thigh muscle was also similar to skin, and significantly higher than that of the control group(p〈0.05). The level of γ-linolenic acid of lipids from chicken breast muscle was highest in the group, which had been fed the EPO 0.5%, followed in order by EPO 0.7%, 0.85%, HO 0.5% and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken breast muscle between the control and treatment groups(p〈0.05).

This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at 4 ± 1.0℃. Seed size ranged 0.44 ± 0.07 × 0.61 ± 0.04 ㎜, and weight of 1,000 seeds was 51.78 ± 0.021 ㎎. Thus the seed was classified as ‘dwarf seeds’. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at 20℃ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be 20℃ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in GA3 and kinetin for 24 hours. Especially, 200~500 ㎎/L GA3 treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as GA3 was thought to be a useful method for raising seedling uniformly.

한국에서 자생군락이 비교적 큰 3지역에서 채집한 달맞이꽃 종자의 필수 지방산을 Capillary column gas chromatography로 분석하였다. 각 지방산의 조성 함량은 palmitic acid가 6.19-6. 73%, stearic acid가 1.84-l.99%, oleic acid 가 6.73-9.10%, linoleic acid가 74.41-75.53%이었다. 특히 prostaglandin의 전구체인 GLA는 청주가 9.14%, 제천이 9.32%이고, 안동이 8.31%이었다. 따라서 한국의 자생 달맞이꽃은 GLA자원으로 유용한 것으로 생각된다.