비피도박테리아 증식인자인 1,4-dihydroxy-2-naphthoica cid 생산 유산균주 LP2가 선행연구에서 자연치즈로부터 분리되었다. LP2 균주는16S rRNA 유전자 염기서열 분석에 의한 균주동정에서 Lactobacillus plantarum(99% 일치율)과 가장 높은 상동성을 나타내어 Lactobacillus plantarum LP2 균주로 명명하였다. LP2 균주의 배양 상등액은 Helicobacter pylori KCTC 12083 균주에 대하여 항균활성을 나타내었으며 이는 생산된 DHNA에 기인하는 것으로 추측되었다.

농산물 중 hexaconazole 분석을 위해, GC-NPD를 이용하여 다종농약 다성분 분석법을 적용하고자 본 연구를 수행하였다. Hexaconazole의 다종농약 다성분 분석법 유효성 검증은 직선성, 정확성, 정밀성, 검출한계 및 정량한계로 하였다. 0.025~5.0 mg/L의 농도에서 검량선의 상관계수(R2)는 0.999이상의 우수한 직선성을 보였다. 상추에 0.04~4.0 mg/kg을 첨가한 농약의 회수율은 89.42~94.15%였고, RSD는 7.78%이하의 재현성을 나타냈다. 검출한계는 0.04mg/kg였고, 정량한계는 0.11 mg/kg였다. 일간 및 일내 정밀도는 각각 2.42~3.49%와 4.90~7.78%였다. 본 연구결과에서 다종농약 다성분 분석법을 이용한 hexaconazole분석은 매우 정확하고, 높은 재현성을 보여, 농산물 중 hexaconazole 분석에 적용할 수 있을 것으로 판단된다.

이 연구는 패류가 다량으로 양식되고 있는 남해안 일부해역 해수의 세균학적 위생안전성을 평가하기 위하여 조사해역 내에 영향을 줄 수 있다고 판단되는 해수 14곳을 선정하여 2014년 3월부터 10월까지 총 6차례 조사를 실시하였으며 그 결과는 다음과 같다. 해수는 수온이 평균 21.2℃, 염분농도는 32.43‰, pH는 7.92, 용존산소는 7.83 mg/L으로 조사되어 우리나라 수질환경기준으로 볼 때 1등급으로 조사되었다. 전도도는 44,600 us/cm, 탁도는 1.93 NTU로 조사되었다. 남해안 일부 조사해역 해수의 위생학적 안전성을 조사한 결과 대장균군은 평균 < 1.8~790 MPN/100 mL, 90번째값이 168.1 MPN/100 mL, 기하학적 평균이 12.8 MPN/100 mL으로 나타났고, 분변계대장균은 < 1.8~790 MPN/100 mL,90번째 값이 50.5 MPN/100 mL, 기하학적 평균값이 6.5 MPN/100 mL로 조사되어 정착성 수산물 생산해역 위생관리기준 중 청정해역의 기하학적 평균 기준치인 14 MPN/100 mL를 넘지 않아 청정해역으로 나타났다. 그러나 5월과 7월에 43 MPN/100 mL를 초과하는 시료가 35.7%와 28.6%로 KSSP 기준치인 10%를 초과하여 계절에 따라서 다소 차이가 있는 것으로 나타났다. 조사해역은 폐쇄성 내만으로 적은 오염원에도 크게 영향을 받을 수 있으므로계절에 따라서 철저한 해역의 관리가 요구되었다.

본 연구에서는 소셜미디어 콘텐츠를 소프트웨어 시스템을 이용하거나 정성적으로 분석함으로써 삭카린나트륨에 대한 소비자 인식도를 조사하고자 하였다. 첫 번째로, 1년(2013.7~2014.6)의 기간 동안 네이버와 카페에서 작성된 글들을 수집하였으며, 수집된 글들은 무료 텍스트 분석 소프트웨어인 TONK를 사용하여 분석되었다. 블로그와 카페에서 작성된 글들은 주로 삭카린나트륨을 사용하지 않은 제품에 대한 것으로 파악되었으며, 기타 높은 단맛과낮은 열량을 보이는 삭카린나트륨의 특성, 식품첨가물 제거법에 대한 내용이 있는 것으로 파악되었다. 두 번째로, 네이버 트렌드 검색 서비스를 이용하여 삭카린나트륨에 대한 검색량 증가 구간에 발생한 언론보도 사건을 조사하였다. PC 검색량의 경우 총 11개 증가 구간 중 6개의 구간에서, 모바일 검색량의 총 12개 증가 구간 중 8개의 구간에서 뉴스에서 삭카린나트륨 사용허용 품목 확대에 대해 보도한 것으로 나타났다. 세 번째로 식품의약품안전처의 삭카린나트륨 사용 허용품목 확대 발표를 보도한 연합뉴스의 댓글을 분석하였다. 공감수 100개 이상인 댓글의 내용을 분석 결과 정부 불신을 비판하는 댓글들이 가장많았으며, 그 외 관련 제품의 가격 비판 댓글과 기업 불신을 드러내는 댓글들이 파악되었다. 시판 제품들의 표시사항 분석 결과, 삭카린나트륨을 첨가하지 않은 제품들은 삭카린나트륨 무첨가라는 표시를 강조하고 있는 것으로 조사되었다. 본 연구 결과 소비자들은 삭카린나트륨의 사용 확대에 관심이 있으며, 사용 확대 보도시에 검색량이 증가하였다. 따라서, 매체들은 삭카린나트륨 사용 확대에관하여 보도를 할 때에 과학적인 근거에 바탕하여 확대배경이나 적정 섭취량 등을 함께 알리는 것이 소비자의 올바른 인식에 도움을 줄 것이다.

본 연구는 학교급식위생에 중요한 역할을 하는 조리종사자의 위생관리수행도에 도움이 될 수 있는 기초위생자료 제공을 위해 급식소의 일반사항, 위생교육에 대한 조리종사자의 태도, 그리고 위생관리수행수준의 관계를 규명하였다. 경남지역에 소재하고 있는 초ㆍ중ㆍ고등학교급식소 23곳의 조리종사자 185명을 대상으로 데이터를 수집하고 분석한 결과, 급식운영형태에서는 직영보다 위탁이 위생관리 수행수준이 더 높게 나타났으며, 하루제공 끼니수 에서는 1식이 2식, 3식을 하는 집단보다 위생관리 수행수준이 더 높은 것을 알 수 있었다. 조리종사자의 위생교육 태도에 따른 위생관리 수행수준 차이를 살펴본 결과,위생교육에 대한 태도가 좋을수록 위생관리 수행도가 높아짐을 알 수 있었다. 본 연구의 결과로부터 급식소의 일반사항과 학교 조리종사자의 위생교육에 대한 태도는 위생관리 수행수준을 결정하는 중요한 요인임이 밝혀졌다.

본 연구는 경기도, 인천 및 서울 지역에 있는 50 곳의 가정 내 주방을 대상으로 미생물학적 오염 정도에 대해 조사하여 가정 내 주방 위생 관리 실태를 평가하고자 하였다. 행주, 냉장고 칸, 도마의 대장균군과 다양한 병원성미생물의 존재 여부에 대한 실험을 실시하였다. 주방기구의 대장균군을 분석한 결과, 행주는 4.8 ± 1.84 log CFU/100 g, 검출률 94.0% (50건 중 47건)의 값을 나타내었고, 냉장고 칸은 4.11 ± 1.65 log CFU/100 cm², 검출률 96% (50건 중48건), 도마는 4.04 ± 1.53 log CFU/100 cm², 검출률 90.0%(50건 중 45건)로 나타났다. 병원성 세균의 오염 실태를 조사한 결과, E. coli는 행주에서 6.0%, 냉장고 칸에서 2.0%의 검출률을 보였으며, Listeria monocytogenes 같은 경우에는 냉장고 칸에서 4.0%의 검출률을 보였다. Salmonellaspp.과 Campylobacter jejuni의 경우에는 모든 시료에서 검출되지 않았으나 Staphylococcus aureus의 경우에는 행주,도마, 냉장고 칸에서 각각 42.0%, 24.0%, 28.0%로 모두높은 검출률을 보여 위생관리 소홀의 심각성을 나타내었다. 가정 내 주방용품 및 기구의 미생물학적 분석 결과 전반적으로 위생상태가 불량한 것으로 평가되었으며 행주 및 도마, 냉장고 칸에서 위생지표세균인 대장균군과 병원성 세균인 황색포도상 구균의 높은 검출률을 보여 가정 내주방의 위생관리가 잘 되고 있지 않음을 시사하였다. 본연구를 통해 가정 내 주방에서의 위생 관리와 사용실태에 대한 인식이 변화되어 가정에서도 위생 관리의 중요성이 강조되어야 할 것이다.

This study was designed to provide the basic data of an effective nutrition education for desirable lifestyle and dietary habits to improve the nutritional status for the elderly by investigating health-related lifestyle, dietary habits, nutritional knowledge, and food intake of the elderly. The subjects included 58 elderly men and 146 elderly women. 35.8% had no more than an elementary school education. Most subjects (54.4%) were widows/widowers. Most subjects (71.0%) made over 150,000 won. 52.5% of subjects lived in houses. Smoking, drinking, exercise, the average sleeping time were significantly difference between the gender (p<0.05). In terms of dietary habits, our results showed that 80.9% of respondent eat regularly meals, including breakfast (83.5%). Both sexes prefer soft and salty food. Women enjoys more spicy and salty food compared with men and then shows meaningful difference (p<0.05). Their favorite meals are soup, stew, salad and boiled vegetables. Compared to women, men have a higher rate of correct answers about questions related to nutrition knowledge as showing significantly difference (p<0.05). In a study of dietary habits, they take in carbohydrates with the highest percentage and following by vegetables and fruits. The amount of meat, first and egg they eat is more than the previously. Eating meat is higher men as showing significant difference (p<0.05). In summary, nutritional status for the elderly shows healthy lifestyle and diet about half of those and most them try to live healthy life in future. It is hope that the elderly may need to learn proper nutrition knowledge for healthy lifestyle and nutrition education and counseling for building up healthy lifestyle and desirable dietary habits. Furthermore, it is necessary to start work to establish a baseline nutritious evaluation for the elderly and at a time to study the development of standard eating tool proven reliability and validity, consequently to provide a basic framework for the evaluation of nutritional status.

1. 우리나라 쌀 소비량은 지속적으로 감소하고 있으나 쌀 가 공식품의 매출은 꾸준히 증가하는 추세에 있다. 2. 이에 본 연구는 농촌진흥청 농식품 소비자 패널조사 자 료(703가구)를 이용하여 최근 5년간 쌀 가공식품 구매패턴의 변화를 파악하고자 하였다. 이를 위하여 쌀 가공식품을 밥류, 죽류, 면류, 떡류, 방류, 과자류, 주류, 음료류, 조미류, 가루류 10종류로 분류하고, 쌀 가공식품의 종류별, 월별, 구매장소별 구매가구 수, 구매금액, 구매빈도 등 전반적인 구매패턴의 변 화를 살펴보았다. 3. 향후 소비 증가가 예상되는 쌀 가공식품의 종류와 향후 요구되는 관련 연구과제를 제안하였다.

This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50=21.12~28.93 μg/mL) and 2,2’-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) (IC50=28.18~52.55 μg/mL) radical scavenging ability with reducing power (IC50=59.91~93.64 μg/mL) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (≥60%). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (≥1,100 μM TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents (R2=0.71~0.79), but total phenolic contents were not correlated with NO inhibition and ORAC (R2=0.35~0.43). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

Traditionally, mistletoe is known as an effective anti-cancer medicinal plant, and lectin is recognized as a major component with cytotoxic and immuno-stimulant activity in mistletoe. A Korean mistletoe lectin (KML) has specificity to galactose and galactosamine and is distinguish from European mistletoe lectin (EML). When we examined the concentration of lectin in mistletoe originated from five different types of host trees, the result indicate that the lectin concentration is variable depending on the host tree. Noticeably, mistletoe from chestnut tree contains ten folds higher lectins than that of an oak tree. We also tested the concentration of KML and crude extract (KM-110) of Korean mistletoe that shows 90% cytotoxicity in L5178Y-ML25 lymphoma cell. In addition, the cells show 90% and 70% viability by the treatment of two neutralizing antibodies of KML, 9H7-D10 and 8B11-2C5 neutralization effect with two monoclonal antibodies of KML, 9H7-D10 and 8B11-2C5. Therefore, the result expected that the mistletoe contain some other cytotoxic components except lectin. Finally, the production of TNF-α and IL-6 by RAW 264.7 cells stimulated with lectin free-crude extract (LFKM-110) following neutralization by 9H7-D10 monoclonal antibody shows higher than that of lectin containing-crude extract (KM-110). These results suggest that the Korean mistletoe lectin ha a great potential to be developed as therapeutic agent of cancer.

The aim of this study was to investigate the effect of food neophobia on restaurant image, customer satisfaction, and loyalty in ethnic restaurants. A self-administered survey for data collection 581 customers who visited an ethnic restaurant in August, 2015. Statistical analyses included descriptive analysis, t-test, ANOVA and regression analysis for SPSS 21.0. The finding of the study indicated that the differences of food neophobia score in demographic characteristics showed significant differences by age, occupation, dining-out frequency, and source of dining-out information, not by gender. The mean value of food neophilic group was significantly higher than food neophobic group in all items of restaurant image, overall satisfaction and customer loyalty. The regression analysis showed that tangible aspect, price aspect, and food aspect of restaurant image had positive effects on overall satisfaction and customer loyalty however employee service showed different result by groups. The finding of the study offer marketing strategies for ethnic restaurants to induce customer revisit.

Environment-friendly agricultural products are consumed widely, but little is known about consumers’ acceptability. The objectives of this study were to 1) determine consumers’ acceptability of cucumber when consumed raw, 2) determine differences in appearance liking between environment-friendly and conventionally grown cucumber samples, and 3) analyze the physicochemical properties of cucumber. White dadagi, gasi, non-pesticide mini cucumber, organic gasi, and organic Joseon cucumber samples were evaluated for consumers’ acceptability using a nine-point hedonic scale; White dadagi, organic Joseon, and organic gasi cucumber samples showed the highest acceptability scores. However, there were three consumer segmentations differing their degree of cucumber liking and preference. Consumers liked the appearance of organic gasi best. Consumers’ preference of cucumbers did not change upon evaluation of the acceptability of the appearance of whole fruit with and without a label indicating an environment-friendly status. Additionally, the physicochemical properties of the cucumbers—including color (skin and flesh), length, circumference, weight, water activity, brix, and texture (hardness and fracturability)—were measured. All measurements showed significant differences among samples, with the exception of water activity and hardness. White dadagi was 3.8 brix, which was the highest among cucumbers, followed by non-pesticide mini cucumber and organic Joseon. White dadagi, organic Joseon, and organic gasi showed greater fracturability than the other cucumber samples.

A high-yield Korean rice cultivar cv. Boramchan and Hanmaeum, and rice cultivar for use in staple cv. Hopum were prepared and investigated for their physiological characteristics. Water content, water holding capacity, amylose content, damaged starch content, particle size, and pasting properties of the rice flours were measured. Moisture content of the Boramchan and Hanmaeum, made under wet and dry milling conditions, were as follows: wet conditions (14.79% and 13.56% respectively) and dry conditions (7.98% and 7.14% respectively). Water holding capacity of the Boramchan and Hanmaeum made by wet milling condition was 236.67% and 231.35%, respectively. Water holding capacity of the samples made by dry milling condition showed a higher score compared with other samples. The amylose content of Hopum, Boramchan, and Hanmaeum made by dry milling condition were 19.12%, 19.55% and 19.59%, respectively. Damaged starch contents of the samples made by wet milling showed a lower score. Final viscosity of Hopum, Boramchan, and Hanmaeum made by wet milling condition was 2,604, 3,052, and 2,917 cp, respectively. In this study, the results indicated that the super-yield Korean rice flour made by wet milling condition tends to show a lower water holding capacity, damaged starch contents and particle size, as compared to controls. However, a setback of the super-yield Korean rice flour was a higher score as compared to the controls, regardless of the milling conditions.

The purpose of this study was to investigate the eating habits and nutritional knowledge of elementary school students in regards to seafood in school meals. More than 80.6% of elementary school students reported an ‘average’ preference for seafood. While ‘taste’ and ‘healthy food’ were common reasons to prefer marine products, ‘unpleasant taste and smell’ was a common reason not to prefer seafood. Students in general stated ‘eating if possible’ as their attitude towards seafood in school meals, 38.1% responded ‘eating all’ as their intake level. There was a significant difference between preference for seafood by gender [boys, 3.44; girls, 3.64 (P<0.01)]. Also, students significantly difference preferred some types of seafood over others. The students who enjoyed eating seafood had more regular and balanced diets and a better attitude toward dietary life and health than did the counterparts who did not prefer seafood. A significant difference (P<0.05) in knowledge existed according to gender; girls had a higher level of knowledge than did boys.

During the Joseon period, kimchi was mostly made by heating the ingredients. Since salt was pricey at the time, in order to save salt and time, people used a method that involved destroying the cell wall by heating the vegetables. However, this method is no longer passed down. Thus, in this paper, we re-discovered how kimchi was made through heating while analyzing the recipes for kimchi during the Joseon period. There were 27 kinds of kimchi made through blanching. To keep the vegetables from becoming soggy, 2 kinds of kimchi were made by putting the ingredients in potassium aluminum sulfate water and 3 kinds were made through blanching the ingredients in limewater. There were 7 kinds of kimchi made by heating in vinegar, 5 kinds by boiling the ingredients, 6 kinds by stir-frying the ingredients, 2 kinds by stir-frying the ingredients with salt, and 3 kinds by steaming the ingredients. In order to eradicate unwanted germs, leaving only Lactobacillus, 25 kinds of kimchi were made by draining the boiled mixture. A total of 17 kinds of kimchi were made by heating the kimchi pot with compost including that of horses. For elders with weak teeth and poor digestion, 7 kinds of kimchi were made after heating, including 3 kinds of sukkkakdugi (cubed radish kimchi made with boiled radish). 3 kinds of chaekimchi (julienned kimchi) and 3 kinds of chaekkakdugi (kimchi with julienned radish) for elders existed as well.

This study attempted to find an efficient method for the preparation of high-purity galactooligosaccharides (HP-GOS) using β-galactosidase and yeast fermentation. GOS prepared using Lactozym 3000L showed the greatest enhancement in total GOS of the six β-galatosidases tested. GOS alone achieved 51% conversion of initial lactose. GOS production was enhanced by fermentation with commercial yeast (Saccharomyces cerevisiae); its concentration reached 71% after 36h fermentation with 8% yeast. Component sugar analysis with HPLC indicated that HP-GOS fermented with S. cerevisiae showed significantly increased levels of 4’/6’-galactosyllactose and total GOS as well as a significantly decreased glucose level. HP-GOS facilitated the growth of Lactobacillus sp. (L. acidophilus and L. casei) and Bifidobacterium sp. (B. longum and B. bifidum). In sum, high-purity GOS has been successfully produced through both an enzymatic process and yeast fermentation. GOS encourages the growth of bacteria such as Lactobacillus and Bifidobacterium that may be beneficial to human gastrointestinal health.

The influence of aging on the flavor precursors and volatile compounds of top round beef was studied. The concentrations of free amino acids, nucleotides, creatine, dipeptides, and volatile compounds were measured after top round from Hanwoo was aged at 4℃ for 21 days. The amount of free amino acids in top round significantly increased with the increase of aging period. There was no effect of aging on the concentrations of adenosine monophosphate or inosine in top round. The inosine monophosphate content of top round significantly decreased with age, while the hypoxanthine content increased. The concentrations of creatine, carnosine, and anserine in top round were not influenced by aging. In total, 24 volatile compound were identified in aged, cooked top round. Of these, the quantities of aldehydes (propanal, pentanal, hexanal, heptanal, octanal, and nonanal), hydrocarbons (pentane and octane), 2-butanone, ethyl acetate, and pyridines (4-ethynyl-pyridine and 4-acetyl-pyridine) significantly increased after aging. We conclude that the flavor of top round can be improved by aging.