Achieving cost-effective and defect-free graphene sheets is highly desirable for sensor devices. Aiming this, few-layer graphene (~ 3) sheets are prepared by an electrochemical exfoliation with [NMP] [ HSO4] electrolyte (i.e., Bronsted acidic ionic liquid). A novel approach for the effective exfoliation of graphene sheets is demonstrated by (i) simultaneously applying a constant potential through an electrochemical cell (with different electrolyte concentrations) and (ii) together with sonication. The exfoliated graphene sheets are characterized through state-of-the-art techniques and sprayed on a glass substrate at optimum conditions. Thus, the transparent conducting sensor device is fabricated with a suitable contact electrode and used for ammonia vapor sensing and the sensor performances are highly dependent on the concentration of the ionic liquid used during the electrochemical exfoliation. The sensing response and limit of detection for the exfoliated graphene-based film were calculated as 3.56% and 432 ppb, respectively. Further studies indicated that the fabricated sensors are more selective towards ammonia molecules with quick response and recovery times.

ROK Navy Landing Ship Fast(LSF-II) is equipped with special equipment that is not compatible with other combat ships due to special missions. So there are almost no maintenance capabilities except for simple repair parts replacement and maintenance parts. The researcher determined that the way to solve these problems was to apply Performance Based Logistics (PBL), and reviewed the cases of PBL applications abroad and domestically. To confirm the current maintenance capability, we visited LSF-II operation unit to identify maintenance capabilities for each mounted equipment, and interviews with operators and maintenance practitioners confirmed the limitations of outsourcing maintenance and the need to apply PBL. In order to analyze the effect of PBL application, the measure of effectiveness and measure of performance were selected based on the opinions of LSF-II operation/maintenance practitioners and PBL experts and the practical experience of this researcher. A survey was conducted on operation/maintenance practitioners and professional personnel. Based on the survey results, the effect of applying PBL was analyzed using the AHP technique, and an efficient PBL application plan was proposed for LSF-II.

기존의 스타크래프트II 내장AI는 미리 정의한 행동 패턴을 따르기 때문에 사용자가 전략을 쉽게 파악할 수 있어 사용자의 흥미를 오랫동안 유지시키기 힘들다. 이를 해결하기 위해, 많은 강화학습 기반의 스타크래프 트II AI 연구가 진행되었다. 그러나 기존의 강화학습AI는 승률에만 중점을 두고 에이전트를 학습시킴으로써 소수의 유닛을 사용하거나 정형화 된 전략만을 사용하여 여전히 사용자들이 게임의 재미를 느끼기에 한계가 존재한다. 본 논문에서는 게임의 재미를 향상시키기 위하여, 강화학습을 활용하여 실제 플레이어와 유사한 AI을 제안한다. 에이전트에게 스타크래프트II의 상성표를 학습시키고, 정찰한 정보로 보상을 부여해 유동적 으로 전략을 변경하도록 한다. 실험 결과, 사용자가 느끼는 재미와 난이도, 유사도 부분에서 고정된 전략을 사용하는 에이전트보다 본 논문에서 제안하는 에이전트가 더 높은 평가를 받았다..

전쟁 시 각국은 고대로부터 마비전을 추구하여 적의 중심을 지향하여 최소의 희생으로 승리하고자 하였다. 또한 특수작전부대는 합동작전의 핵심부대로 각국은 특수작전부대를 증가하고 있는 추세이다. 따라서 본 연구의 목적은 전쟁양상의 변화와 더불어 마비전이 진화함에 따라 전쟁 시 마비전 사례분석을 통해 특수작전부대의 역할을 도출하고, 미래 다영역 작전 시 마비전 수행을 위한 특수작전부대의 운용방향을 제시하는 데 있 다. 연구 방법은 문헌연구를 통한 질적연구와 사례를 분석 후 비교하였다. 연구범위는 제2차 세계대전으로부터 걸프전, 이라크전을 분석하였고 미래 운용방향은 미국의 다영역작전 시 특수작전부대 운용을 연구하여 한국군에 적용하였다. 연구 결과, 마비전은 초기의 물리적 마비로부터 물리적 마비 +체계무력화+심리적 마비로 진화하며 발전하였고, 특수작전부대는 마비 전 수행에 결정적으로 기여하였으며 그 중요성은 더욱 증가하고 있음을 확인할 수 있었다. 따라서 한국군 특수작전부대는 미래 다영역작전에서 도 마비전을 수행하기 위해 지속적인 마비전 수행개념과 이를 위한 조직 편성 및 무기체계의 발전이 요구되고 있다.

Along with other heavy metals, arsenic (As) is one among the substances most harmful to living organisms including humans. Owing to its morphological similarity to phosphorus, the uptake of As is influenced by photosynthesis and the phosphorus uptake pathway. In this study, we varied arsenic exposure and light intensity during nutrient solution cultivation of lettuce (Lactuca sativa L.) to determine the effect of these two factors on arsenic uptake, lettuce growth, and electron transfer in photosystem II. In the treatment exposed to 30 μmol L-1 of arsenic, the shoot arsenic concentration increased from 4.73 mg kg-1 to 18.97 mg kg-1 as the light intensity increased from 22 to 122 μmol m-2 s-1. The water content and ET2o/RC of the shoots were not affected by arsenic at low light intensity; however, at optimal light intensity, they decreased progressively with arsenic exposure. Increased light intensity stimulated the growth of plant roots and shoots; contrarily, the difference in growth decreased as the concentration of As exposure increased. The results of this study suggest that the effect of As on plant growth is dependent on light intensity; in particular, an increase in light intensity can increase the uptake of As, thereby affecting plant growth and As toxicity.

This study aimed to develop a solid self-nanoemulsifying drug delivery system (solid-SNEDDS) to enhance the formulation of ketoconazole (KTZ), a BCS Class II drug with poor solubility. Ketoconazole, which is insoluble above pH 3, requires solubilization for effective delivery. This SNEDDS comprises oil, surfactant, and co-surfactant, which spontaneously emulsify in the gastrointestinal tract environment to form nanoemulsions with droplet sizes less than 100 nm. The optimal SNE-vehicle composition of oleic acid, TPGS, and PEG 400 at a 10:80:10 weight ratio was determined based on the smallest droplet size achieved. This composition was used to prepare liquid SNEDDS containing ketoconazole. The droplet size and polydispersity index (PDI) of the resulting liquid SNEDDS were analyzed. Subsequently, solid-SNEDDS was fabricated using a spray-drying method with solidifying carriers such as silicon dioxide, crospovidone, and magnesium alumetasilicate. The physicochemical properties of the solid-SNEDDS were characterized by scanning electron microscopy and powder X-ray diffraction, and its solubility, droplet size, and PDI were evaluated. In particular, the solid-SNEDDS containing ketoconazole and crospovidone in a 2:1 weight ratio exhibited significantly enhanced solubility, highlighting its potential for improved medication adherence and dissolution rates.

Hypertension caused by high-fat and high-salt diets is is a well-known significant risk factor for cardiovascular and cerebrovascular diseases. In this study, to confirm the relationship between hypertension and immune cells, angiotensin (Ang) II was administered to Dahl salt-sensitive (SS) rats and Dahl salt-resistant (SR) rats. Then the expression of immune cells and the proinflammatory cytokines were compared between the SS and SR rats. It was observed that after administration of Ang II (50ng/kg/min) for three weeks, blood pressure was increased in the SS rats, but there was no significant change in the SR rats. In addition, the expression of T helper (Th) cells and Th 17 cells in the spleen and the expression of Th cell Rorγt and regulatory T regulatory (Treg) cells in the peripheral blood mononuclear cells did not show a significant difference between the two experimental groups even after the administration of Ang II.IL-1β expression was significantly increased in the kidney tissue of the SS rats, while there was no significant difference in the IL-6 expression in all the experimental groups. The results of this study suggest that Ang II induces hypertension by stimulating IL-1β secretion from renal macrophage in SS rats.

PURPOSES : This study aims to perform a quantitative analysis of Forward Collision Warning and crash frequency using heavy vehicle driving data collected in expressway driving environments, and to classify the driving environments where Forward Collision Warnings of heavy vehicles occur for accident-prone areas and analyze their occurrence characteristics. METHODS : A bivariate Gaussian mixture model based on inter-vehicle distance gap and speed-acceleration parameters is used to classify the environment in which Forward Collision Warning occurs for heavy vehicles driving on expressways. For this analysis, Probe Vehicle Data of 80 large trucks collected by C-ITS devices of Korea Expressway Corporation from May to June 2022. Combined with accident information from the past five years, a detailed analysis of the classified driving environments is conducted. RESULTS : The results of the clustering analysis categorizes Forward Collision Warning environments into three groups: Group I (highdensity, high-speed), Group II (high-density, low-speed), and Group III (low-density, high-speed). It reveals a positive correlation between Forward Collision Warning frequency and accident rates at these points, with Group I prevailing. Road characteristics at sites with different accident incidences showed that on-ramps and toll gates had high occurrences of both accidents and warnings. Furthermore, acceleration deviation at high-accident sites was significant across all groups, with variable speed deviations noted for each warning group. CONCLUSIONS : The Forward Collision Warning of heavy vehicles on expressways is classified into three types depending on the driving environment, and the results of these environmental classifications can be used as a basis for building a road environment that reduces the risk of crashes for heavy vehicles.

Air conditioner filters purify the air of indoor environments by removing air pollutants and supporting the efficiency of the unit’s cooling function. However, an air conditioner filter can become a microenvironment in which some fungi can grow as dust continues to accumulate and favorable humidity conditions are formed. Fungal growth in air conditioner filters could lead to fungal allergies or fungal diseases, in addition to emitting a foul odor. In an effort to understand what species causes this malodorous problem, we investigated the diversity of fungi found in air conditioners. Fungi were sampled from the collected air conditioner filters and grown on DG18 agar media. After purification for pure isolates, species identification was undertaken. Colony morphology was observed on PDA, MEA, CYA, and OA media. Microstructures of fruiting body, mycelia, and spores were examined using a light microscope. Molecular identification was performed by PCR and sequencing of PCR amplicons, and molecular phylogenetic analysis of sequenced DNA markers, including the Internal Transcribed Spacer (ITS), the 28S large subunit of the nuclear ribosomal RNA (LSU rDNA), the β-tubulin (BenA) gene, the Calmodulin (CaM) gene, and the DNA-directed RNA polymerase II subunit 2 (RPB2) gene. Through this identification process, we found two fungal species, Aspergillus miraensis and Dichotomopilus ramosissimus, which are unrecorded species in Korea. We will now report their morphological and molecular features.

본고는 『헨리 6세』 2부와 3부가 가부장제의 이념을 바탕으로 남녀 간의 뚜렷한 역할 구분에 기초하고 있다고 보고 전통적인 성 역할에 따르지 않는 마가렛과 같은 여성의 양상을 살핀다. 주로 마가렛의 용맹성, 전투력과 성적 매력과 방종이 주변 남성 인물의 가부장적인 가치에 대립되는 모습을 추적한다. 마가렛과 일리노어 등이 권력을 추구하는 과정에서 남성들, 특히 영국 남성을 대표하는 기사도적 영웅들과 대항하는 양상을 보이는데 이 여성 전사들이 대표하는 가치가 어떻게 남성들의 이상적 영웅주의나 부자간의 혈연 중심 관계에 대립하는지도 살핀다. 일부 남성 인물들이 감정에 치중하는 연인의 모습을 보이며 남성간의 유대를 약화시키는 결과에 이르는 아이러니한 상황 또한 살펴본다. 남성들이 이들 여성과 관련하여 남성성을 잃게 되는 과정을 분석함으로써 『헨리 6세』 2부와 3부에 담긴 가부장제의 양상과 여성 전사의 전복적 힘을 충실히 고찰하고자 한다.

In this paper, an alternative inventory policy that trades off the bullwhip effect at an upstream facility with cost minimization at a current facility, with the goal of reducing system wide total expected inventory costs, when external demand distributjon is autocorrelated, is considered. The alternative inventory policy has a form that is somewhere between one that completely neglects the autocorrleation and one that actively utilizes the autocorrelation. For this purpose, a mathematical model that allows us to evaluate system wide total expected inventory costs for a periodic review system is developed. This model enables us to identify an optimal inventory policy at a current facility that minimizes system wide total expected inventory costs by the best tradeoff of the bullwhip effect at an upstream facility with cost minimization at a current facility. From numerical experiments, it has been found that (i) when the autocorrelation is negative, the optimal policy is one that actively utilizes the autocorrelation, (ii) when the autocorrelation is small and positive, the optimal policy is one that neglects the autocorrelation, and (iii) when the autocorrelation is large and positive, the optimal policy is somewhere between one that actively utilizes the autocorrelation and one that neglect the autocorrelation.

This paper attempts to show that the word sì 巳(祀) used after the negative wù 勿 or wú 毋 functions as a “Vintransitive” (all the abbreviations and references used in this paper are given at the end of Part I). As such, it forms a VP, 勿/毋V1V2, where V2 is always one of the six “Type-A ritual Vs”—one being dăo 禱 ‘to pray’, the rest given in the paper. The semantic relationship between V1 (巳/祀) and V2 is closely examined. There are also inscriptions in which V1 is used before yú 于, a “multidirectional” preposition. This is labelled as “Nloc in the sense of ‘in, at’ (not ‘to, for’)” in the construction given in the title. The paper accounts for its motive principle. The paper argues that 巳/祀 is a Vaction meaning “conduct sì-providential ritual”. It was done at a place to seek divine direction and guidance from the deity or Power believed to have dwelt in situ. Originally presented in Takashima (2009a), this paper delves further into linguistic, philological, and cultural aspects of the 巳-ritual couched in the VP, 勿/毋V1(=巳/祀)V2. A major reason for its use is to contrast the 巳-ritual with other rituals and sacrifices. The details will be explained with examples. Part I covers “Examination I: Zhū Shēngyù’s Questions and Zhāng Yùjīn’s Interpretation”. The paper answers the former and evaluates the latter. Part II begins with “Examination II: Qiú Xīguī’s Interpretation” and ends with the conclusions of the entire paper.

In this study, we detected the presence of residual pesticides in 341 agricultural products collected from local food outlets in western Gyeonggi Province. Residual pesticides were detected in 105 (30.8%) samples. Six samples exceeded the legal limits for residual pesticides, resulting in a non-compliance rate of 1.8%, which was slightly higher than the average non-compliance rate of 1.4% in the last three years. Among the tested agricultural products, only fruits and vegetables were found to have pesticide residues, with 24 of 34 fruits (a detection rate of 70.6%) and 81 of 277 vegetables (a detection rate of 29.2%) testing positive. In total, 59 types of pesticides, including acetamiprid, which was detected 208 times, were detected and had a detection range of 0.01–2.38 mg/kg. Among the 105 agricultural products containing pesticide residues, a single pesticide was detected in 62 samples (59%) and two or more pesticides were detected in 43 samples (41%). In particular, 14 pesticides were detected in the same sample of peaches; dinotefuran was detected 21 times. Upon examining the toxicity of the detected pesticides, Class III pesticides (moderate toxicity) were detected 44 times (21.2%) and Class IV pesticides (low toxicity) were detected 164 times (78.8%). Class I, II, and III pesticides with fish toxicity were detected 68 (32.7%), 14 (6.7%), and 126 times (60.6%), respectively. Upon examining the exposure to high-frequency pesticide components detected five or more times, the hazard index was found to be ≤2.8%. Accordingly, the hazard of residual pesticides based on dietary intake was deemed insignificant.

Sulfur and nitrogen co-doped carbon dots (NSCDs) were quickly synthesized by the microwave-assisted method from triammonium citrate and thiourea. NSCDs showed a quantum yield of 11.5% with excitation and emission bands at 355 and 432 nm, respectively. Also, a fluorescence quenching was observed in the presence of Pb(II) ions, and the as-synthesized CDs were used as a sensitive probe for detecting Pb(II) in water and food samples. The results showed the optimal conditions for Pb(II) determination were CDs concentration of 0.02 mg mL− 1 at pH 6.0–7.0 and an incubation time of 20 min. The relative fluorescence intensity of NSCDs was proportional to Pb(II) concentrations in the range of 0.029–2.40 and 2.40–14.4 μmol L− 1 with a correlation coefficient (R2) of 0.998 and 0.955, respectively, and a detection limit of 9.2 × 10– 3 μmol L− 1. Responses were highly repeatable, with a standard deviation below 3.5%. The suggested method demonstrates the potential of a green, fast, and low-cost approach for Pb(II) determination in water, tea, and rice samples with satisfying results.

이 연구는 일본 규슈 북쪽 서단(西端)에 위치한 작은 도시지만, 제1,2 차 세계대전에서 구(舊)일본 해군의 군사적 요충지 역할을 맡은 군항도시 사세보(佐世保)가 관광도시로 전환되는 그 과정의 설계와 실천 과정에 기반을 두고 있다. 1983년 개장한 나가사키(長崎) ‘오란다무라(Holland Village)’는 지역경제에 많은 파급효과를 가져왔고, 1992년 개장한 ‘하우 스텐보스(Huis Ten Bosch)’는 관광도시로의 전환에 있어 ‘결정체’와 같 은 역할을 했다. 오랜 기간 군항도시의 역할이 지속된 사세보는 전전기 (戦前期)와 전후기(戦後期)를 거치며 어두운 전쟁의 체험과 역사적 흔적 을 남겼다. 하지만 사세보 시민들이 염원하던 ‘평화산업항만도시’로의 전 환에 있어 결정체 역할을 한 ‘하우스텐보스’의 개장은 마치 어두운 전쟁 의 역사의 흔적을 지우듯 나가사키의 역사와 문화라는 옷을 입혀 그 부 지위에 자리 잡았다. 하우스텐보스가 조성된 부지는 전전기에는 해군병 교 하리오(針尾)분교가, 전후기에는 인양자들의 숙소이자 인양 원호국이 자리잡고 있었던 곳이었다. 이처럼 사세보의 어두운 역사의 땅 위에 하 우스텐보스가 지어지며 군항도시에서 관광도시로 전환된 과정을 흥미롭 게 살펴보고자 한다.

Ultra-violet (UV) light is one of abiotic stress factors and causes oxidative stress in plants, but a suitable level of UV radiation can be used to enhance the phytochemical content of plants. The accumulation of antioxidant phenolic compounds in UV-exposed plants may vary depending on the conditions of plant (species, cultivar, age, etc.) and UV (wavelength, energy, irradiation period, etc.). To date, however, little research has been conducted on how leaf thickness affects the pattern of phytochemical accumulation. In this study, we conducted an experiment to find out how the antioxidant phenolic content of kale (Brassica oleracea var. acephala) leaves with different thicknesses react to UV-A light. Kale seedlings were grown in a controlled growth chamber for four weeks under the following conditions: 20°C temperature, 60% relative humidity, 12-hour photoperiod, light source (fluorescent lamp), and photosynthetic photon flux density of 121±10 μmol m-2 s-1. The kale plants were then transferred to two chambers with different CO2 concentrations (382±3.2 and 1,027±11.7 μmol mol-1), and grown for 10 days. After then, each group of kale plants were subjected to UV-A LED (275+285 nm at peak wavelength) light of 25.4 W m-2 for 5 days. As a result, when kale plants with thickened leaves from treatment with high CO2 were exposed to UV-A, they had lower UV sensitivity than thinner leaves. The Fv/Fm (maximum quantum yield on photosystem II) in the leaves of kale exposed to UV-A in a low-concentration CO2 environment decreased abruptly and significantly immediately after UV treatment, but not in kale leaves exposed to UV-A in a high-concentration CO2 environment. The accumulation pattern of total phenolic content, antioxidant capacity and individual phenolic compounds varied according to leaf thickness. In conclusion, this experiment suggests that the UV intensity should vary based on the leaf thickness (age etc.) during UV treatment for phytochemical enhancement.

식물구계학적 특정종은 식물상 정보를 이용한 지역환경을 평가하는 주요 수단이며, 종의 선정과 평가는 식물구계 정보에 의거해서 이루어진다. 본 연구는 이러한 식물구계에 대해 학술적 진의를 규명하고자 하였다. 식물구계 관련 핵심문헌 7편을 검토하였으며, 식물구계 적용 근거를 비교 검증하였다. 1919년의 Nakai 울릉도 식물상 조사보고서는 식물구계에 관련한 일반적인 지리구분(지방)을 처음으로 기재했다. Oh는 1977년에 한국의 유관속식물에 대한 식물지리구계도를 처음으로 제시하였 다. 1978년에는 Lee & Yim이 중부와 남부를 가르는 구계도를 제시했고, 그 밖에 많은 연구자들에 의해 식물구계 경계는 조정되고 개정되었다. 마침내 2018년에는 Lee & Yim (1978)에서 변형된 식물구계학적 특정종의 식물구계도가 채택되었다. 그런데 이러한 식물구계들은 연구자 간에 차이가 있었고, 구계 지표 식물의 불일치성도 확인되었다. 그럼에도 그런 상이성에 대한 근거는 찾을 수 없었다. 결론적으로 도식화된 모든 식물구계도는 선험적으로 그은 임의 선에 의존하고 있었다. 본 연구는 식물구계학적 특정종이 환경영향평가 및 국가 식물상 평가를 위한 과학적 수단으로서 그 실효성과 실체성을 확보하기 위해 다음의 두 가지 생태학적 전략을 제안했다 : 종분포 고유성(nativeness) 정보를 바탕으로 하는 식물상 및 식물구계의 성취와 서식처 기반(habitat-based) 식물상 장기모니터링 시스템의 구축.

본 연구는 OECD TG No. 458, 22Rv1/MMTV_GR-KO 전사 활성화 분석법을 포함한 세포 기반 분석법을 사용하 여 식품 및 생활용품에 포함된 파라벤과 트리클로산의 인 간 안드로겐 수용체를 매개하는 내분비계 교란 가능성을 확인하는 것을 목표로 한다. 4가지 파라벤(메틸-, 에틸-, 프 로필-, 부틸-)은 OECD TG No.458에서 AR 길항제로 확 인된 반면, 파라벤의 AR 길항 효과는 S9 간 분획물이 있 는 경우 나타나지 않았다. 트리클로산 역시 AR 길항제로 분류되었으며, 트리클로산에 의해 유도된 AR 길항 효과 는 S9 간 분획물이 존재할 때 제 1상+2상 대사에서 유의 하게 감소되었다. 파라벤과 트리클로산에 의해 유도되는 AR 길항 기전은 세포질 내 AR 이량화를 차단하여, 리간 드 결합 AR이 핵으로의 전위를 억제함으로써 AR 매개 내분비 교란 효과를 나타냈다. 이러한 결과는 4가지 파라 벤과 트리클로산이 AR 이량화 저해를 통한 AR 길항 효 과를 나타내는 AR 매개 내분비 교란 가능성을 가지고 있 으나, 간 대사 효소가 존재할 경우 내분비 교란 효과는 감 소됨을 시사한다.

본 연구는 코칭 전공 대학원생들이 코칭을 전공으로 선택한 이유를 대학원생 들의 관점에서 살펴보고 유형화하여 이들이 실질적으로 코칭 전공 대학원에서 갈망하는 요구를 이해하기 위한 목적으로 진행되었다. 이를 위하여 인간의 주관 적 인식을 분석하기에 적합한 Q방법론을 통해 연구 문제를 분석하였다. 본 연구 에서는 국내 코칭 관련 학과 대학원 석·박사과정 재학 또는 졸업한 사람들 중 목적표집 방법으로 선발된 총 21명의 P표본을 대상으로 40개의 진술문을 구성 하여 연구가 진행되었다. 연구 결과 총 4개의 유형으로 분석되었고 유형Ⅰ은 ‘경 제적 수입보다 사람을 중요시하는 기여형’, 유형Ⅱ는 ‘네트워킹보다 스스로를 중 요시하는 자아 계발형’, 유형Ⅲ은 ‘스펙을 통해 코칭의 학문 발전을 중요시하는 준비형’, 유형Ⅳ은 ‘코칭 철학을 믿는 긍정적 영향력 발휘형’으로 나타났다. 본 연구 결과는 유형화된 대학원생의 코칭전공 선택에 관한 주관성 특성을 바탕으 로 이후 국내 코칭의 학문적 발전을 위해 코칭 관련 학과 대학원의 체계적인 교 육과정 방향성의 틀로 활용될 수 있으며, 나아가 국내 코칭 정착과 발전에 필요 한 기반이 될 수 있을 것이라 기대한다.

Insect cuticle is an extracellular matrix formed primarily from two different biopolymers, chitin and protein. During each molt cycle, a new cuticle is deposited simultaneously with degradation of the old cuticle by molting fluid cuticle degrading-enzymes, including epidermal chitinases (CHTs). Insect CHTs, belonging to family 18 glycosylhydrolase (GH18), have been classified into at least eleven subgroups based on phylogenetic analyses, and group I (CHT5) and group II (CHT10) epidermal CHTs present in molting fluid. In this study we report the physiological function of MaCHT5 and MaCHT10 in the Japanese pine sawyer, Monochamus alternatus. RNAi for either MaCHT5 or MaCHT10 resulted in larval-pupal and pupal-adult molting defects, in which the insects were unable to shed completely their old cuticle and died entrapped in their exuviae. Furthermore, TEM analysis revealed a failure of degradation of the old cuticle in both MaCHT5- and MaCHT10-deficient pharate adults. In the old pupal cuticle, the chitinous horizontal laminar and vertical pore canal essentially remained intact in the endocuticular layer. These results indicate that both CHTs are required for turnover of the chitinous old cuticle, which is critical for completion of insect molting. We also discuss the possible function of two spliced variants of MaCHT10, MaCHT10a and MaCHT10b.