Genetically modified (GM) papaya (Carica papaya L.) line 55-1 (55-1), which is resistant to papaya ringspot virus infection, has been marketed internationally. Many countries such as the European Union, Japan, and Korea have a mandatory safety assessment, approval and labeling regulations for GM foods. Thus, there is a need for specific methods for detecting 55-1. In this study, we established a real-time PCR detection method applicable to 55-1 for a variety of papaya products. The limit of detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% (weight per weight [w/w]) for homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The 55-1 event-specific detection method observed parallelism (r2>0.99) between the concentration of line 55-1 cultivars and Ct values obtained in amplification plots at concentrations of 0.005-10% for SunUp DNA and 0.01-10% for Rainbow DNA. The method was applicable to the qualitative detection in various types of processed products (cocktail fruit, dried fruit, juice, etc.) containing papaya as a main ingredient. Monitoring papaya products for the presence of GM papaya were demonstrated using a P35S and T-nos real-time PCR detection method but no amplification signals were detected.

The seed shattering played a key role in the crucial step of rice domestication. Because it has been important to increase the yield human had to select the rice varieties and species with low shattering degree. The shattering habit of rice is considered to be under the relatively simple genetic control compared with other characteristics related to domestication. Several recessive genes associated with the formation of an abscission layer, sh2, sh4 and sh-h on chromosomes 1, 3 and 7, have been reported. In addition, the grain shattering of rice is considered to be caused by seed abscission. The morphology of the abscission layer can differ in many different rice varieties that show varying degrees of shattering. Accordingly, it is important to elucidate the molecular mechanism to determine why some varieties do not have abscission layers and have an easy-shattering trait. In this study, analysis of QTL for grain shattering was performed to determine the location of QTLs on the whole chromosomes of rice. Also, we tried to construct a physical map for qPs6

The White backed planthopper (WBPH), Sogatella furcifera (Horvath) is one of the serious insect pests in rice growing region in Asia. When rice is attacked by the insect it releases secondary metabolites for self-defense. In this study, we identified WBPH-mediated compounds from a cross ‘Cheongcheongbyeo/Nagdongbyeo’ doubled haploid (CNDH). The compounds were located in chromosome. Leaves and stem of CNDH lines were infected by 2∼3 insta of 3 weeks WBPH and samples were extracted by 90% methanol. Extracted compounds were analyzed through HPLC. TLC was used in separating the target compounds. QTL analysis of compound was done using winQTLcart 2.5 program. Chrysoeriol was highly contained in Cheongcheongbyeo. QTL location is found on chromosome by winQTLcart 2.5. QTL analcited with compound7 was detected on chromosome 4, 7 and 12. qFla4 was detected on chromosome 4 in RM280-RM6909 at LOD 3.5 with 30% of variation. qFla7 was detected on chromosome 7 in RM248-RM1134 with LOD 3.0 with 30% of variation. qFla12 was detected on chromosome 12 in RM1226-RM12 with LOD 2.7 with 40% of variation. Cochlioquinone was detected on chromosome8, qFla8 in RM23230-RM3689 with LOD 2.5 with 30% of variation. Chrysoeriol and Cochlioquinone separated to condition of (Chloroform: Methanol:1-Butanol:Water=4:5:6:4). Separated compounds were analyzed by LC/MS and NMR. These results, investigation is being done to ditermine how the secondary metabolites come lead to pathways of genes and its effect on WBPH relation.

The whitebacked planthopper(WBPH), Sogatella furcifera is a serious pest of rice. The nymphs and adults suck phloem sap which causes reduced plant vigor, stunting, yellowing of leaves, delayed tillering in rice. This study was conducted to identify the optimum screening time for improved WBPH-associated QTL analysis and to develop the markers for use in breeding WBPH resistance. Resistance after 7 days infestation was observed in 100 lines(83.3%), after infestation for 14 days, resistance was observed in 14 lines(11.7%), and after infestation for 21 days, resistance was observed in 10 lines(8.3%). However, no after 14 days infestation was as similar as normal distribution in WBPH resistance. QTLs associated of the resistance detected in four regions on qWBPH1 and qWBPH8 in the intervals marker. After 7 days of infestation, the qWBPH1 was located in the interval RM3482-RM11966 and RM3709-RM11694 with LOD 4.0 and RM3709-RM11694 with LOD 3.5. After 14 days of infestation, The qWBPH1 was located in the interval RM3709-RM11694 with LOD 3.3. and RM3709-RM11694 with LOD 3.3. After 21 days of infestation, The qWBPH8 was located in the interval RM17699 with LOD 3.3. The QTLs on chromosome 1 was the most effective RM11694-RM11669 (LOD 3.3, variance 30%). The resistance lines were collected 10 plants of phenotye variation with genotype. The ratios of coincidence were used to determine resistance in 10 plants with phenotypic variation and a genotype of 8 markers. 3 markers were used: RM3482 on chr.1 represented 100%, RM8235 and RM11694 represented 80%, 90%, respectively, RM17699 on chr.8 represented 80% of the coincident ratio. These selected markers will be useful to rice breeding programs interested in new sources of WBPH resistance

Most of the melon(Cucumis melo L.) breeding lines in Korea show andromonoecious (male-perfect flowers) sex expression, which requires laborious hand emasculation to produce the F1 seeds. There is a high demand for developing monoecious (male-female flowers) elite germplasm. The present study was carried out to develop molecular markers for selecting monoecious plants based on the CmACS-7 gene [a locus with 1-aminocyclopropane-1-carboxylic acid synthase(ACS) activity] responsible for ethylene synthesis and sex determination in melon. The full length sequences of the CmACS-7 were cloned from a monoecious inbred ‘Mo23’ and an andromonoecious inbred ‘Am24’. Sequence alignment revealed a major SNP(C170T) in exon1 and 18bp indel in intron4 of the CmACS-7, and a CAPS (SNP-C170T) and SCAR (ID4-18) were developed from the SNP and indel, respectively. A total of 453 F2 plants derived from ‘Mo23’ x ‘Am24’ were determined for their sex expression and genotyped using the SCAR marker. A Mendelian ratio of 3(monoecy): 1(andromonoecy) was observed from the F2 population, and sex type of 449 plants (except for four plants that showed incomplete monoecy) cosegregated with the SCAR marker, demonstrating that CmACS-7 is a single dominant gene conferring monoecy of ‘Mo23’. Allele variation of the CmACS-7 was evaluated by genotyping 114 melon accessions with diverse geographical origins using the CAPS and SCAR. C170T-SNP in exon1 of the CmACS-7 was highly conserved in melon germplasm and perfectly matched with the phenotype, whereas the 18bp-indel mutation in intron4 existed in various forms. The results demonstrated that CAPS marker SNP-C170T can be useful for marker-assisted selection(MAS) of monoecious melon plants

Secondary plant metabolites undergo several modification reactions, including glycosylation and physiological functions. Glycosylation, which is mediated by UDP-glycosyltransferase (UGT), plays a role in the storage of secondary metabolites and in defending plants against stress. In this study, a UDP-glucosyltransferase cDNA was isolated from Brassica rapa hereinafter referred to as BrUGT. It has a full-length cDNA of 1,236 bp that contains a single open reading frame of 834 bp which encodes a polypeptide of 277 amino acid residues with a calculated mass of 31.19 kDa. BLASTX analysis hits a catalytic domain of glycos_transf_1 super family (c112012) that belongs to the glycosyltransferases group 1 with tetratricopeptide (TPR) regions. UGT gene expression analysis showed high mRNA transcripts in pistil, followed by petal, seed and calyx of flower in Brassica rapa. Furthermore, we constructed a recombinant pFLCIII vector carrying the BrUGT gene under the control of ubiquitin promoter and NOS terminator and transformed into rice using Agrobacterium tumefaciens. The UGT overexpressing rice lines were then characterized at the physiological and molecular levels. To further understand the biological function of BrUGT, transcriptional profiling of the gene in transgenic rice lines under cold, salt, PEG, H2O2, ABA and drought stress condition is underway.

Many viruses infect cucurbits. One of the well-known symptoms is mosaic disease. Those that cause mosaic are cucumber mosaic virus (CMV), squash mosaic virus (SqMV), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV) and cucumber green mottle mosaic virus (CGMMV). WMV resistant GM squash was developed many years ago in the United States and it was on the market, but no further information was available by now pertinent to commercial aspect. Usually these viruses are not easily controlled by frequent applications of chemicals that target the insect as carriers of viruses. Therefore, it is necessary to develop commercial varieties possessing resistance against viral diseases. We have developed GM watermelon rootstocks called gongdae, using a coat protein gene of CGMMV as transgene. Those GM watermelon rootstocks showed highly resistant to CGMMV, and have been crossed to get the several BC and T generation. In order to obtain the virus resistant watermelon, watermelon lines were crossed to the selected GM watermelon rootstock. Here, we present the successful watermelon cultivars that show resistance to CGMMV. The resistance must have obtained by transferring the transgene from the GM watermelon rootstock to watermelon line

With the development of next generation sequencing (NGS) technology, the variation of sequences represented as SNP between cultivars becomes available at genome level. The major domestic cultivars with high yield have been developed by breeding of indica and japonica, it is important to localize the region of origin according to the genotype for further characterization of unique features of cultivars. For the localization of SNP at genome level, the paired end sequences of 6 major domestic rice cultivars, Ilmi, Ilpoom, Sulgaeng, Baekjinju1ho, Hwayoung and Woongwang were compared against Japonica and Indica Rice Genomes as reference genomes. The genomic DNAs were prepared from callus tissues and paired-end of the fragments were sequenced with NGS Sequencer, Illumina HISeq2000. About 50x coverage of paired-end sequences were trimmed according to the quality of the sequences, and errors were corrected with statistical analysis of kmers of 15. The trim-corrected sequences were mapped and variants were analyzed against reference genomes. The overall change rate of Ilmi against Nipponbare IRGSP 1.0 and Indica BGI 93-11 reference genomes were 0.92 base/1kb (1/1,079 base) and 8.09 base/1kb (1 base/123 bases), respectively. Among 6 cultivars, overall rate of Baekjinju1ho showed the lowest overall change rate of 0,53 base/1kb, and Hwayoung showed highest frequency of 0.92 base/1kb. Compared to high level in the range of change rate of 7.0-9.3 base/1kb against indica, domestic cultivars showed lower range of change rate 0.2-3.3 base/1kb with unique local high peak against japonica genome depend on the chromosomes. Compared to assembly of genome sequences, the variation of nucleotides compared to reference sequences is much faster and simple to characterize the genotype. The types of variation and the effect on functional categories will be presented.

Unfavorable environmental stresses are major limiting factors that affect plant productivity. Plants perceive and respond adaptively to an abiotic stress condition, and the adaptive process is controlled mainly by phytohormone, consequently, changing in gene expression pattern. Transcriptional regulator ABF3 (Abscisic acid response element Binding Factor 3) mediating the ABA-responsive gene expression plays important roles in drought and temperature tolerance. Here, we report an event of drought tolerant GM gourd in which abf3 was inserted in the genome. For drought stress experiment, T0 plants were self-pollinated and back-crossed to select cultivars for drought stress experiment. The drought tolerant GM gourds were selected for last 3 years and have been crossed to get the several BC and T generation consecutively. In this year, BC3T1, F1 (Bak x GM gourd), BC1F1 and control plants were subjected to drought stress, that is no watering for 12 days and rehydration afterwards. The GM gourds showed high tolerance to drought while the non-transformed plants were totally dried. When the plants were subjected to rehydration, the GM bottle gourds were completely revived and recovered from the drought stress. Tolerance levels to drought of BC3T1, F1 (Bak x GM gourd) and BC1F1 were 92.5%, 50.0% and 65.0%, respectively.

Bt gene derived from the B. thuringiensis has been used for developing GM crops, and corn, cotton and soybean producing B. thuringiensis toxins have been on the market for last 17 years or so creating a huge GM seed industry. One of the notorious pests in brassica crops is diamondback moth (DBM). In order to protect the insect plague of crops from DBM, 4-5 billion dollars have been wasted annually for applying integrated measures in worldwide. Major prevention is use of pesticides that may build the contamination level of chemicals in the ground and this practice threats the environment and ecosystem. An alternative is to develop GM brassica crops and therefore we have developed GM cabbages resistant DBM using bt gene. Lots of T0 cabbages were tested for resistance and independent GM cabbages resistant to DBM were selected. Molecular analysis was conducted to find if GM cabbage holds one copy transgene and intergenic insertion. We found an independent GM cabbage and it contained a singly copy of the transgene without disturbing the insertion site. This one called C95 line with an status of event have been self-crossed for two generation (T2). Also we are working the development of GM cabbage with different vector that contains bar gene as a selection marker. So far 17 T0 cabbages have been obtained by bar selection.

Fresh market tomato cultivars are divided largely based on fruit color appearance (red or pink), which is attributed by the trait of peel. It had been reported that mutation of the Slmyb12 gene suppresses synthesis of yellow-colored flavonoid (naringenin chalcone) in peel and causes pinkish tomato fruit. Whereas wild-type tomato plants synthesizing naringenin chalcone produce yellow-colored peel, which resulting in the fruit appearance to be red. The present study was performed to investigate the association between the Slmyb12 and fruit color of domestic tomato inbreed lines. A SCAR marker was developed from an Indel mutation site (72bp insertion in exon3) of the Slmyb12, and tested on 22 and 18 red and pink-fruited inbred lines, respectively. Unexpectedly, the results showed that all inbred lines tested had wild-type Slmyb12. The full length sequences of the Slmyb12 were cloned from two inbred lines (FCR1 and FCP1), but the sequence alignment did not identify any nucleotide variations within this gene. Furthermore, scanning of SNPs between FCR1 and FCP1 using SolCAP Tomato SNP array) found no SNPs for Slmyb12. To delimit the genomic region of the gene conferring fruit color of domestic tomato lines, we are analyzing SNPs in the genes adjacent to the Slmyb12 using an F2 population derived from FCR1 x FCP1. So far, one SNP located at 1,750kbp downstream from the stop codon of Slmyb12 was mapped using 54 F2 plants and 83% of phenotype-marker association was revealed, demonstrating that the fruit color is controlled bySlmyb12 indeed, or other neighboring gene(s) involved in the pathway of naringenin chalcone synthesis. Further study with more SNPs will clear up this question.

Rice cultivation by direct seeding allows reduced labour and production costs in addition to other benefits. However the success of this rice production method can be limited by uneven fields with poor drainage or heavy rainfall at sowing leading to early flooding conditions slowing germination and hindering crop establishment. Hence, the need to improve tolerance to anaerobic conditions in both rainfed and irrigated rice ecosystem after direct seeding. In this study QTL analysis was performed to identify QTLs associated with tolerance derived from Vietnamese variety Tai Nguyen (TN) under anaerobic conditions during germination. The population derived from a cross between TN (tolerance indica lines) and Anda (susceptible japonica), was used for collection of phenotypic data based on the survival rates of the seedlings at 21 days after sowing under 10cm of water. A total of 286 F2:3 families of the population were used for QTL mapping and the genotyping was carried out with the infinium 6K SNP-chip based on the illumina infinium platform. Two significant QTLs associated with the AG trait were detected on chromosomes 1 and 11, respectively. In Particular, the QTL on chromsome 1 had an LOD score of 7.45 and R2 of 14.21%. We plan to confirm the identified QTLs in further studies and develop varieties with improved anaerobic germination ability using advanced backcross lines.

Pear (Pyrus spp.) is one of the most important temperate fruit species in the world. The identification of Pyrus spp. based on analysis and comparison of their phenotypes has been causing a number of synonyms and homonyms among Pyrus spp. For accurate identification, genotype analysis with molecular marker such as microsatellite is necessarily to use. However, It is difficult to share and compare of microsatellite profiles obtained among different laboratories because no effort has made to standardize equipment or protocols. A strategy for data comparison by dint of reference to the alleles detected in well-known cultivars will be helpful for co-work with laboratories and developing international databases. Thus the study was performed to compare results between laboratories of microsatellite DNA profiling for reproducibility and standardize allele scoring by defining reference alleles efficiently. In this study, ninety pear species including forty-four comparison Asian pears, six of comparison suspicious Asian pears and reference pears, seven basic pear species, and twenty-seven Korean native pear cultivars to development of a standard set of SSR reference alleles. Twelve primers covering most of pear genome were chosen, which are CH02b10, CH03g07, CH04e03, CH03d12, EMPc117, CH01f07a, EMPc11, CH01d09, GD147, CH01d08, CH05c06, and GD96 located in pear chromosome of 2, 3, 5, 6, 7, 10, 11, 12, 13, 15, 16, and 17 respectively. Genotyping was conducted with Fragment Analyzer. After genotyping, a set of defined standard alleles based on reference pear cultivars can facilitate data comparison among laboratories and will permit the improvement of a common international database.

Crops are exposed to various environmental stresses. These have been affecting the growth of crops, resulting in the severe loss of agronomic production in many countries. Therefore, development of new varieties of resistant crops is required to assure the desired productivity of crops in stress conditions. In this study, a putatively stress-related gene BrTSR53 was isolated from Brassica rapa. The BrTSR53 is 481 bp long and contains ORF region of 234 bp. The expression of BrTSR53 was determined by quantitative real-time PCR analysis. After 3 hr, the highest quantities of mRNA were revealed in cold and salt stress treatments. In drought stress treatments, there was the highest expression after 36 hr. Therefore, it was confirmed that the ORF in BrTSR53 should be a gene that confer increased resistance to B. rapa growing in different stress conditions. The ORF region of BrTSR53 gene was cloned into an expression vector, pYES-DEST52, and a new protein with molecular weight of 13 kDa was detected by western blot analysis. Also, stress tolerance tests showed that BrTSR53-ORF transgenic yeast exhibited increased resistance to the salt stresses compared with the control. In conclusion, the present data predicts that novel ORF in BrTSR53 can serve as an important genetic resource for abiotic stress resistance.

To reveal the linkage relationship between the Ms locus, a restorer-of-fertility gene for cytoplasmic male-sterility (CMS) caused by CMS-S cytoplasm in onion (Allium cepa L.) and previously reported molecular markers linked to the Ms locus, 11 recombinants selected from 4,273 segregating plants originating from the cross between male-sterile maternal and male-fertile paternal lines were analyzed. Results showed that genotypes of a codominant marker, jnurf12, were perfectly matched with the male-fertility phenotypes in all recombinants, but that this marker was not applicable in diverse breeding lines due to multiple band patterns. For the development of more reliable markers, a 12-bp indel was identified from the sequences which were obtained by genome walking, and was used to develop a simple PCR marker which was designated jnurf13. When 104 diverse breeding lines containing CMS-S cytoplasm were analyzed with the jnurf13 marker, male-fertility phenotypes of all breeding lines were perfectly matched with marker genotypes. To our surprise, phenotypes of 153 breeding lines containing CMS-T-like cytoplasm were also matched with genotypes of the jnurf13 marker which was linked to the Ms locus for the CMS-S system. Furthermore, phenotypes of four F2 populations containing CMS-T-like cytoplasm co-segregated perfectly with jnurf13 genotypes. Allelic segregation distortion was detected in two F2 populations using the jnurf13 maker. The results of this study were in conflict with a previous model for inheritance of fertility restoration in the CMS-T system. Therefore, we proposed a new model based on the data analyzed with the jnurf13 marker, which was in linkage disequilibrium with restorer-of-fertility genes for both CMS systems.

Numerous environmental stresses, such as abiotic and biotic stresses, cause significant yield loss in crops and can significantly affect their development. Un the field conditions, crops are exposed to a variety of concurrent stresses. Combined high temperature and linked diseases can cause considerable damage that eventually leads to crop death. Hence, this study was conducted to characterize the genes encoding the nucleotide-binding site (NBS) motif obtained from transcriptome profiles of two cabbage genotypes with contrasting responses to heat stress. We selected 80 up-regulated genes form a total of 264 loci, among which 17 were confirmed to be complete and incomplete members of the TIR-NBS-LRR (TNL) class families, and another identified as a NFYA-HAP2 family member. Expression analysis using qRT-PCR revealed that 8 genes showed significant responses to heat shock treatment and F. oxysporum infection. Additionally, in the commercial B. oleracea cultivars with resistance to F. oxysporum, Bol007132, Bol016084, and Bol030522 genes showed dramatically higher expression levels in the F. oxysporum resistant line than the intermediate and susceptible lines. The results of this study may facilitate the identification and development of molecular markers based on multiple stress resistance genes related to heat and fungal stress under field conditions in B. oleracea.

기상조건의 변화로 우리나라에 벼멸구의 발생이 점차 증가하며 피해가 커지고 있다. 다양한 벼멸구 저항성 유전 자를 가진 벼 품종의 육성이 필요하다. 본 연구는 Marker-assisted Selection(MAS)을 통한 벼멸구 저항성 유전자 Bph3 보유 벼 품종 육성을 위해 Bph3 연관마커 탐색을 수행하였다. 각각 서로 다른 벼멸구 저항성 유전자를 가진 9개 품종의 DNA를 추출한 후 6번 염색체의 Bph3 유전자 인근의 8개의 SSR 마커를 가지고 PCR을 수행하였다. 8개 SSR 마커중 bph4를 제외한 나머지 유전자와 다른 위치의 밴드를 나타내는 RM586이 가장 적합한 마커로 판단되었 다. 선발된 RM586의 MAS 능력을 검정하기 위해 벼멸구에 감수성인 벼 품종 새누리와 Bph3 유전자를 보유한 저항 성 벼 품종 BG367-2를 교배하여 F1을 육성하였고 다시 새누리를 반복친으로 여교배 하여 BC1F1 80개체를 얻었다. 80개체를 벼멸구 저항성 검정 결과 1:1의 저항성과 감수성 분리비를 보였으며, 이들 개체의 DNA를 분리하여 RM586 마커로 PCR 후 전기영동 했을시 저항성 개체와 감수성 개체는 서로 다른 크기의 밴드를 형성 하였다. RM586로 생성된 PCR 산물의 크기는 2% agarose gel로 쉽게 분석이 가능하다. 따라서 RM586은 Bph3 유전자를 가 지는 벼멸구 저항성 품종 육성에 MAS를 사용하는데 적합한 마커로 확인되었다

김정문 알로에 제주농·공장에서는 알로에 베라와 알로에 아보레센스를 중심으로 450여종의 알로에가 보존되어 져 오고 있으며, 알로에 종 일부는 전시용으로도 활용되고 있다. 전세계 500여종의 알로에가 알려져 있는 가운데 제주농·공장의 알로에 보존 현황은 원산지가 아님에도 불구하고 많은 종수를 보유하고 있으며 보존 또한 잘 이루 어지고 있다. 또한 변종 및 아종과 더불어 교잡종 등의 분류가 제대로 이루어진다면 분류군은 증가하리라 예상된 다. 따라서 제주농·공장에 보존되고 있는 다양한 알로에 종을 김정문 알로에의 경제적 자원으로서 활용하기 위해 서 이들의 유연관계를 분자생물학 기술을 활용하여 분석하고 품종 구분에 대한 기술을 확보하는 것이 필요하다. 이에 따라 김정문 알로에 제주농·공장에 보존되어 있는 품종 중 경제성이 있을 것으로 판단되는 35종의 품종을 대 상으로 하여 Operon 사의 OPF 프라이머 5종, OPD 프라이머 15종을 사용하여 RAPD 분석을 수행하였다. 이를 통해 품종별 유연관계를 분석하였고, 10개의 그룹으로 알로에가 분류될 수 있음을 확인하였다. 이와 같은 연구를 통해 품종판별용 RAPD 마커 제작도 가능할 것으로 기대된다.

In contrast with wild species, cultivated crop genomes consist of reshuffled recombination blocks, which occurred by crossing and selection processes. Accordingly, recombination block-based genomics analysis can be an effective approach for screening target loci with agricultural traits. We propose the variation block method, a three-step process for recombination block detection and comparison. The first step is to detect variations by comparing short-read DNA sequences of the cultivar to a reference genome of the target crop. Next, sequence blocks with variation patterns are examined and defined. The boundaries between the variation-containing sequence blocks are regarded as recombination sites. All the assumed recombination sites in the cultivar set are used to split the genomes, and the resulting sequence regions are named as variation blocks. Finally, the genomes are compared using the variation blocks. The variation block method identified recurring recombination blocks accurately and successfully represented block-level diversities in the publicly available genomes of 31 soybeans and 23 rice accessions. The practicality of this approach was demonstrated by the identification of a putative locus determining soybean hilum color. We suggest that the variation block method is an efficient genomics method for recombination block-level comparison of crop genomes. We expect that this method holds the prospect of developing crop genomics by bringing genomics technology to the field of crop breeding.

Carotenoid isomerase (CRTISO) catalyzes the isomerization of prolycopene to all-trans-lycopene in the carotenoid biosynthetic pathway. We isolated a full-length promoter region of CuCRTISO from Citrus unshiu. We determined if the promoter encoded organ-specific or developmental-specific expression, and identified possible cis-acting promoter elements. The full-length promoter and two truncated versions were fused to the β-glucuronidase (GUS) gene and transformed into Arabidopsis thaliana. Transgenic lines expressing the full-length promoter (pCiso-Prom1) and truncated promoters (pCiso-Prom2 and pCiso-Prom3) showed the same developmental and organ-specific activity. GUS expression was detected in the cotyledon and root at 5 and 10 days after germination, mature leaf, and anther. The CuCRTISO promoter contained several cis-acting elements involved in hormonal and environmental stress. Drought stress or abscisic acid treatment did not induce GUS expression in any transgenic lines. Heat stress induced GUS expression in the pCiso-Prom1 line; this promoter construct contains the heat-stress responsive element (HSE). Ethylene and cold-stress treatments induced GUS expression only in the pCiso-Prom3 line, although all transgenic lines contained the same cis-acting ethylene and low-temperature response elements. which could indicate the existence of unknown repressor element(s) in the CuCRTISO promoter. These studies indicate that CuCRTISO promoter activity is regulated in a developmental and organ-specific manner that responds to heat, cold, and ethylene. These results provide new insights into the role of cis-acting element(s) in CuCRTISO promoter activity. (This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2010-0007627 and 2009-0094059), and by Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and Korea Forest Service (KFS), Republic of Korea)