저밀도 리포단백질 수용체 관련 단백질 5(LRP5)는 간과 췌장을 포함하여 많은 조직에서 발현하며 아포리포단백질 E와 결합한다. 이와 같은 LRP5 유전자의 체내 기능을 규명하기 위하여 LRP5 유전자가 결손된 생쥐를 개발하였다. 먼지 LRP5 genomic DNA는 TT2 ES 세포로부터 분리하였으며 LRP5 유전자의 엑손 18에 neo 유전자를 삽입한 vector를 구축하고 TT2 ES 세포에 도입하였다. 178개의 G418 내성을 보인 세포 중 상동유전자 재조합에 의하여 targeting vector가 LRP5 유전자 위치에 삽입된 clone은 3개였다. 키메라 생쥐는 상실배기 수정을 ES 세포와 응집시켜 생산하였으며 생산된 키메라 생쥐는 C57BL/6 생쥐와 교미를 유도하여 heterozygous를 얻었다. 또한 이들 heterozygous간의 교배에 의하여 LRP5 유전자 결손 생쥐를 생산하였다. 이러한 생쥐는 LRP5 유전자의 체내 기능연구에 있어서 모델로 이용될 것으로 생각된다.

PSS hetero 돼지를 이용하여 PSS와 관련된 유전자를 cloning 하여 유전자 구조를 분석하고 세포내의 유전자의 존재를 확인하여 PSS 돼지의 유전양식을 밝히고자 실시되었고, 그 결과를 요약하면 다음과 같다. 615번 amino acid가 N과 n에서 각각 arginine과 cysteine으로 존재함을 확인할 수 있었다. 그리고 6번 염색체(PSS 관련유전자)로부터 우성유전자 N과 열성유전자 n이 각각 유전자 좌위(locus)에 대립 유전자(alleles)로 존재함을 확인할 수 있었으며, 이러한 alleles로 존재함으로써 각각의 유전자가 나뉘어져 유전됨을 알 수 있었다.

This study was to evaluate effects of agar, sodium alginate and carrageenan on quality of Yugwa(Busuge) base. In the base preparation agar, sodium alginate and carrageenan were added 0.0, 0.1, 0.5, 1.0 and 3.0%(w/w), respectively. Volume, shape, hardness, color(L, a and b value), crude lipid content and sensory evaluation(taste and crispness) of the Yugwa base was measured. Volume of the base was higher than control in case of 0.1-0.5%(w/w) agar, sodium alginate and carrageenan, of which sodium alginate was the highest. Shapes were similar to control. Hardness and crude lipid content was decreased proportional to amount of addition of the three seesweed polysaccharides, the whiteness(L-value) was increased but the yellowness(a-value) and the redness(b-value) decreased. Taste and crispness were increased in the case of 0.1-1.0%(w/w) of sodium alginate, but agar and carrageenan decreased.

The purpose of this study was to evaluate the effects of pulsed ultrasound on wound healing and observe during the wound healing process the distribution of mast cells according to histopathologic findings. Eighty Sprague Dawley rats which were divided into 4 groups received full thickness skin wounds on the back. Each of the 5 animals was sacrificed immediately and then sacrificed again 1, 3, 6, and 12 days after injury. Specimens from the wounds were removed during healing and routinely processed with a hematoxylin-eosin stain and a toluidine blue stain. The authors then observed the distribution of mast cells under a light microscope. The results of this study were as follows: The rate of wound healing and the length of the wounds of the pulsed ultrasound group II was significantly faster than group I on day 6 and day 12 (p<.001). Group III showed the most significant effect after12 days (p<.001). Group IV also showed a significant effect at 12 days (p<.01). A low-intensity ultrasound .5 resulted in a fast healing rate. During the wound healing process mast cells had a tendency to decrease in the acute inflammatory phase. During the wound healing process mast cells were thought to contribute to the healing of the wound.

본 연구는 체외성숙/체외수정 유래의 돼지 난자를 이용하여 체외발달시 배양액의 종류나 교체에 따른 영향을 구명하고자 수행하였다. mNCSU-23에서 체외성숙시킨 다음 mTBM에서 체외수정시킨 난자를 목적에 따라 두 가지로 나누어 실험한 결과는 다음과 같다. 1. 체외성숙/체외수정란을 NCSU-23에서 배양액 교체없이 7일 동안 배양하거나 CZB에서 4일 배양한 다음 Pig-MEM으로 옮겨서 나머지 3일간 배양한 결과, 난분할율은 배양액간 차이를 보이지 않

본 연구는 돼지 태아 섬유아세포유래 공여세포를 미세주입에 의해 주입 후 재 조합한 핵 이식 배에 대한 배양액, 세포주기의 동기화, 배양시간 및 난자의 활성화에 따른 융합율과 체외발생율에 대해 조사하였다. 핵 이식 배를 NCSU-23, TL Hepes 및 TZM-3 배양액으로 1시간 및 8시간 배양하였을 때 배반포로의 분할율은 각각 15.6%, 14.0%, 15.0% 및 13.9%, 10.5%, 13.3%로서 배양액 및 시간에 따른 분할율의 유의적인 차이

This study was performed to investigate the health status of 600 owner-driver of taxi in Seoul City during from August 1, 2003 to February 28, 2004. The prevalence of subjective symptoms were surveyed by a general health questionnaires such as Todai Health Index (THI) and the Cornell Medical Index (CMI). In the THI health check table, prevalence of the complaints of mental subjective symptom by age was high in the items of fabrication (L), digestion (C), and aggressiveness (F) from 31 to 40 years old drivers and mental subjective symptom by age was high in all items except for tiredness (I), melancholia (N), and hypersensitivity (P) for the 60 years old drivers and showed in the items of melancholia (N) and hypersensitivity (P) for the 40 years old. From the complaints of mental and physical subjective symptoms about work satisfaction in the THI health check table, the strong dissatisfaction showed in the all items except for eye and skin trouble (B). All items except hypersensitivity (P) showed dissatisfaction in prevalence of complains of mental and physical subjective symptoms about work satisfaction in the CMI health check table. From the complains of mental and physical subjective symptoms on salary satisfaction in the THI health check table, the taxi driver who earn the salary of 1 million won showed statistically significant symptom of eye and skin trouble (B) and showed high tendency in all items except for respiratory (A) and digestion (C) systems. It is suggested that the appropriate health control for owner-driver of taxi should be applied to reduce the prevalence of complaints that showed mainly in the irregular working time and sleeping disorders.

As quality becomes a primary leading factor of organizational success, various management strategies have been In-troduced to Improve quality competitiveness Quality competitiveness, however, is difficult to measure and numerous organ-izations are struggl

This study was performed to investigate the characteristics of indoor air quality (IAQ) in 30 public facilities from February to April, 2004 in Seoul. The collected samples are PM10, formaldehyde, and total suspended bacteria in indoor air and measured by the IAQ standard method of Ministry of Environment in Korea(M.E.K). As the results of the survey, the mean concentration of PM10 was 55.19 ㎍/m3 in indoor air and 51.15 ㎍/m3 in outdoor air. The mean concentration of PM10 in the Kindergarten was higher than the IAQ standards by established by M.E.K. The mean concentration of formaldehyde was 43.08 ㎍/m3 in indoor air and 5.05 ㎍/m3 in outdoor air, respectively. All of them were less than IAQ standards of Korea. The concentration of total suspended bacteria in the streamer room was higher than IAQ standards. The concentration of formaldehyde was different according to the year of construction of building. Total suspended bacteria in streamer room was necessary for control. Moreover, this study was for investigation a part of indoor air pollution condition in public facilities. It means that this study can't represent for all of public facilities. Therefore, we suggest that long and middle term country plan for management of IAQ should be established through long-term and continuous investigation of IAQ condition.

This paper is case study for ISO 9001/2000 quality management system certification of social welfare We constructed quality management system for efficient operation for service quality Improvement of welfare hall which is society welfare facilities This

본 연구에서는 경사와 위사의 장력에 기인되는 감성 봉제용 직물의 마찰특성을 KES-FB 계측기를 이용하여 분석하였다. 직물의 마찰특성은 직물표면의 마찰계수, 마찰계수의 평균편차, 그리고 표면요철도를 측정하였다. 이들 표면특성치를 분석하기 위해서 75d/36f PET 필라멘트를 경사로 사용하고 100d/192f PET 필라멘트를 위사로 사용하여 5매 주자직 직물을 Omega와 Picanol 직기에서 각각 제직하고 이들을 같이 연결하여 염색가공 공정을 진행시켰다. 이들 가공된 직물의 직물표면특성은 제직시 측정된 경사와 위사의 장력특성과 함께 분석되어 졌으며 직물의 중앙부위와 양 셀베지 부분의 직물 위치에 따른 표면특성의 차이가 분석되어졌다.