본 연구는 대전, 충청지역 초음파실 종사자를 대상으로 병원감염관리에 관한 설문 조사를 실시하여 분석 하였고, ATP를 이용하여 임상에서 사용되는 초음파 Probe의 오염도를 측정하였으며, 70% 알코올 이용하여 소독한 후 결과를 확인하였다. 기본적인 조사 대상자의 일반적인 특성 및 직종 별 대상자의 특성으로 나누어 학력, 직종, 초음파 전문자격증 보유 유무, 기관 내 감염 담당 부서 및 감염 교육의 여부를 분석하였다. 검사 후 젤 제거 여부 및 방법, Probe의 소독여부 및 변수 별 상관분석을 수행하여 초음파 Probe의 세균감염에 관한 인식 및 세척 및 소독에 대한 수행 정도를 분석하였다. 검사 후 Probe 세척에 관하여 수건을 가장 많이 사용하였으며, Gel 용기는 3개월 이상 교환하지 않는 것으로 나타났다. 70% 알코올 소독 후 ATP 오염도는 1055.4±944.2에서 133.5±93.2으로 감소하였고 통계적으로 유의한 것으로 분석되었다.(ρ<0.01) 병원 균종은 CNS, Gram positive bacillus, Micrococcus species 검출되었다. 이를 해결하기 위해 시중에서 손쉽게 구할 수 있는 70% 알코올을 이용하여 소독을 실시하였으며 소독 후 병원균종은 검출되지 않았다. 연구 결과 초음파실을 관리하는 의료인 또는 의료기사 등은 감염관리에 대한 주기적인 교육과 감염예방에 대한 노력이 필요하다고 볼 수 있으며, 70% 알코올은 병원균 사멸에 효과적인 것으로 나타났다. 따라서 의료기관은 적극적인 병원감염관리 교육을 실시하여 종사자들의 병원감염에 관한 인식을 개선하고, 환자 감염 예방에 기여해야 할 것이다. 또한 본 연구 결과를 적절히 활용한다면 초음파 Probe를 매개로 한 감염 예방에 도움이 될 것으로 사료된다.

DXA 검사는 작은 골량의 변화로 발생하는 생물학적 변화를 가장 잘 반영할 수 있어 임상에서 우수한 성능을 발휘하는 골밀도 검사 장치이다. 검사의 정확도와 정밀도의 유지를 위하여 정도관리가 필수적으로 수행되어야 하지만 팬텀의 제작이 어렵고 상대적으로 고가의 가격이기 때문에 병원마다 보유하지 않고 있는 경우가 많다. 따라서 본 연구에서는 3D 프린터 및 시중에서 손쉽게 구할 수 있는 필라멘트의 내부채움 정도의 변화를 이용하여 교차보정 팬텀을 개발하고 상용화된 팬텀과의 비교 평가를 통해 유용성을 평가하였다. 팬텀의 개발을 위해 ABS, TPU, PLA, 30% Cu-PLA, 30% Al-PLA의 HU을 평가하였으며 각각 내부 채움 10 0%에서 -149.74±2.36, -55.62±7.14, -7.68±3.82, 87.53±1.07, 1795.20±16.15의 HU를 나타내었다. 선형회귀분석이 적용된 3D 프린팅 팬텀의 L1, L2, L3 골밀도는 0.620±0.010 g/cm2, 1.092 ±0.025 g/cm2, 1.554±0.026 g/cm2으로, 기존 팬텀과 통계적으로 높은 관련성을 보였다. 이를 활용한다면, DXA 장치의 적절한 정도관리가 가능할 것이며, FDM 3D 프린팅을 이용한 다양한 의료용 팬텀 제작에 기초 자료로 활용될 것으로 사료된다.

야콘 분말의 이상적인 저장조건을 규명하기 위한 기초자료로서 저장상대습도(11-75%) 및 온도(25℃, 35℃, 45℃)에 따른 생 및 데친 야콘 분말의 흡습에 미치는 영향을 조사하고 그 특성을 모델링하였다. 생 및 데친 야콘 분말의 등온흡습곡선은 type III 곡선을 나타내었으며, 데친 시료의 평형수분함량은 생 시료에 비해 낮은 것으로 나타났다. GAB식으로부터 예측한 대부분의 단분자층 수분함량은 BET식으로부터 예측한 값보다 낮은 것으로 나타났으며, 생 및 데친 시료의 값은 유사하거나 일정한 패턴의 차이는 발견되지 않았다. 실험이 수행된 조건하에서 GAB와 Halsey 모델이 생 및 데친 야콘 분말의 흡습특성을 가장 잘 예측할 수 있는 것으로 확인되었다. 이러한 실험결과는 야콘의 건조가공 및 저장의 최적조건을 도출하기 위한 중요한 자료로 사용될 수 있을 것으로 판단된다.

Burdock powder (BP) has shown diverse functional activities; thus, it seems a good way to increase its consumption by incorporating BP into suitable food products such as cookies. Studies were carried out on cookies prepared by incorporating BP (2%, 4%, 6%, and 8%) into wheat flour. The cookie dough and cookies were evaluated for their physical, chemical, and sensory characteristics. The pH and density of cookie doughs ranged from 6.70-6.85 and 1.19-1.21, respectively, with no remarkable differences. Moisture content and spread factor of cookies appeared to increase with higher content of BP in the formulation but did not show significant differences (p>0.05). For color values of cookie surface, L*, a*, and b*-values significantly decreased as a result of BP substitution (p<0.05). The cookies became crispier as indicated by the reduction in the breaking strength value from 26.71 to 17.83 N. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities were significantly increased (p<0.05) with higher substitution of BP, and they were well correlated. Hedonic sensory results indicated that cookies supplemented with 2% BP received the most favorable acceptance scores for all sensory attributes evaluated. Overall, cookies with BP could be developed with improved physicochemical qualities as well as consumer acceptability.

본 연구에서는 영지버섯 균사 배양 시 헛개나무 추출물의 첨가가 영지버섯의 가나도마난디올의 생합성에 미치는 영향을 분석하기 위하여 수행하였다. 가나도마난디올은 트리터페노이드 계열의 물질이며 영지버섯의 주 요한 생리효능을 가지는 물질 중의 하나이다. 이와 관련하여, 본 연구자들은 선행연구를 통하여 가나도마난디올 이 B16F10 멜라노마 세포의 티로시나제 저해 활성 및 멜라닌 생합성 저해능에 우수한 효과가 있는 것을 확인하 였다. 본 연구에서 영지버섯 균사 배양 시 15% (v/v)의 헛개나무 추출물 첨가하면 영지버섯의 가나도마난디올 생합성이 첨가하지 않은 대조군에 비해 유의적으로 증가함을 HPLC분석을 통하여 확인하였다. 또한, 15% (v/v)의 헛개나무 추출물을 첨가한 영지균사 배양추출물의 B16F10 멜라노마 세포에 대한 멜라닌 생합성 억제 능이 첨가하지 않은 대조군에 비해 유의적으로 증가함을 확인하였다. 또한, 영지버섯 균사 배양 시 헛개나무 추출물 첨가가 미백활성을 가지는 가나도마난디올 생합성 증가뿐 아니라, 액체 및 고체 배양시 균사의 생장도 촉진하는 것을 확인하였다. 이러한 결과들은 헛개나무가 영지버섯균사의 미백활성 물질인 가나도마난디올 생 합성의 증가를 유도하고 이로 인한 영지버섯 균사의 미백 활성이 증가하는 유용한 소재로 사용될 수 있다는 것을 시사한다.

In this study, groundwater flow was analyzed targeting Dae-jeong watershed, which exhibited the largest variations of groundwater levels at the identical elevation points among the 16 watersheds of Jeju Island. The issues of the methods applied in practice were identified and improvement plans were suggested. This groundwater-flow estimates derived by applying hydraulic conductivity values onto zones of equal topographic ground level were found to be quite different from actual measured groundwater flow. Conversely, groundwater-flow estimates that utilized hydraulic conductivity values applied onto groundwater-level equipotential lines indicated relatively lesser divergences from actual measured groundwater flow. The reliabilities of the two approaches were assessed for 60 randomly selected points on DEM (digital elevation model) maps, The method using hydraulic conductivity values applied onto groundwater-level contours turned out to be the more reliable approach for the Dae-jeong watershed in Jeju Island.

Jeju Island relies on subterranean water for over 98% of its water resources, and it is therefore necessary to continue to perform studies on drought due to climate changes. In this study, the representative standardized precipitation index (SPI) is classified by various criteria, and the spatial characteristics and applicability of drought in Jeju Island are evaluated from the results. As the result of calculating SPI of 4 weather stations (SPI 3, 6, 9, 12), SPI 12 was found to be relatively simple compared to SPI 6. Also, it was verified that the fluctuation of SPI was greater fot short-term data, and that long-term data was relatively more useful for judging extreme drought. Cluster analysis was performed using the K-means technique, with two variables extracted as the result of factor analysis, and the clustering was terminated with seven-time repeated calculations, and eventually two clusters were formed.

Background : Achyranthes japonica Nakai (AJ) is a perennial herb with a wide distribution in East Asia including Korea, China, and Japan, and it is mainly used as a medicinal plant. In Korea, AJ has been widely used to control pain and improve symptoms in OA patients. AJ contains several important phytochemicals such as saponins, inokosterone, ecdysterone, and oleanolic acid bisdesmoside. Methods and Results : The aim of this work was to investigate the antioxidant and anti-inflammatory activities of fermented and ethanol extracts of Achyranthes japonica Nakai (AJ). The extracts showed strong reductive power and nitrite scavenging, hydroxyl radical scavenging, superoxide radical scavenging, and DNA damage prevention activities. Treatment of RAW 264.7 macrophages with AJ inhibited lipopolysaccharide (LPS)-induced NO secretion and iNOS expression without affecting cell viability. AJ also inhibited cyclooxygenase 2 (COX-2) expression, leading to the suppression of COX-2-derived prostaglandin E2 production. These inhibitory effects of AJ were accompanied by reduced production of tumor necrosis factor-α and interleukins (IL)-1β, -6, and -10. Furthermore, AJ suppressed LPS-induced phosphorylation of extracellular signal regulated kinase, c-Jun N-terminal kinase, and p38. Moreover, AJ inhibited malondialdehyde production and myeloperoxidase activity in LPS-stimulated RAW 264.7 cells. Conclusion : The antioxidant activity of plants is closely related to their medicinal properties and is widely used as a parameter to determine the bioavailability of medicinal plants. The antioxidant and biological activities of AJ extracts might be due to the synergistic actions of multiple bioactive compounds. It can be concluded that AJ extracts are a potential source of biologically important drug candidates.

Background : ROS produced by oxidative stress damaged endothelial cells, and cause a variety of vascular complications. In diabetic hyperglycemia state, ROS increase. The polyol pathway occur in diabetic complications, the excess glucose is absorbed into the polyol pathway when aldose reductase increased, NADPH changes it to sorbitol. Glutathione (GSH) removes ROS. GSH level is reduced by glutathione reductase, using NADPH as an electron donor. Activation of the polyol pathway decrease NADPH, and GSH also reduced. As a result, ROS is increased. In diabetic hyperglycemia state, Glycolysis increases. Effects of increased glycolysis, protein kinase C (PKC) is increased. NAD(P)H oxidase, stimulated by PKC-dependent pathway, increases ROS in the cell. In this study, we measured the ROS scavenging activity of 5 natural products (Lycii fructus, Astragalus membranaceus, Cassia Tora, Polygonatum odoratum, Rubus Coreanus), to confirm the efficacy as diabetic antioxidants. Methods and Results : We extracted 5 natural product by distilled water and ethanol. DPPH radical scavenging activity was significantly higher in Lycii fructus, Rubus coreanus. ABTS radical scavenging activity was better Rubus coreanus, Lycii fructus, Cassia Tora. In addition to, Rubus coreanus, Cassia Tora, Lycii fructus was comparatively higher reducing power activity than other natural products. And total phenolic and flavonoid contents were much higher in Rubus coreanus compared with other extracts. Conclusion : These results suggest that Lycii fructus, Rubus coreanus can be applied as diabetic antioxidant that prevent vascular complications caused by ROS.

Background : Dioscorea quinqueloba(DQ) is a medicinal herb that is used as an alternative therapy for cardiovascular disease and various medical conditions. The objective of this study was to characterize the antioxidant activities of DQ. Methods and Results : The samples were extracted with Distilled water and analyzed for total flavonoid contents, polyphenol contents, DPPH radical scavenging activity, and ABTS radical scavenging activity. H9c2 cardiomyoblast cells were subjected to H2O2, to study the protective effect of DQ on cell viability, and ROS production. The total amounts of polyphenols and flavonoids, which indicate the antioxidant capabillity of water extracts from DQ were 27.21mg/g and 22.95mg/g, respectively. The DQ water extract showed highest antioxidant activity by DPPH and ABTS scavenging activities. The DQ water extract was protected cells against H₂O₂-induced cell death without any cytotoxicity, as determined by the MTT assay. The DQ water extract also was inhibiting production of intracellular ROS. Conclusion : These observations suggest that DQ can use potentially good natural antioxidant in daily life for possible health benefits.

Background : Osteoclasts as multinucleated cells originate from hematopoietic monocyte/ macrophage precursor cell, shows the bone absorption through the commitment, differentiation, fusion, and bone resorption stages by regulation of M-CSF and RANKL. It has been reported a significant negative correlation between the increase of oxidative stress and the bone density, and when RANKL reaction to the osteoclasts precursor cells is mainly generated ROS is due to increased activity of NADPH oxidase1 (NOX1), and these ROS act as a factor which promotes osteoclasts differentiation. Thus, RANKL signaling process is important that excessive osteoclast formation and differentiation inhibited through the regulation of each step. Methods and Results : F3570 ethanol extract showed relatively high activity at in-vitro antioxidant activity. F3570 water extract inhibited ROS generation in RAW 264.7 cells stimulated with H2O2 and RANKL, even at low concentrations. The inhibitory effect of osteoclast differentiation on F3570 water extract was confirmed that shown through NF-κB pathway, MAPK pathway including ERK and JNK. F3570 ethanol extract is considered to be regulated by the p38 MAPK and the other signaling pathway. Also, F3570 both water and ethanol extract were significantly reduced gene expression such as TRAP, calcitonin receptors and integrin β3 of RANKL-induced mature osteoclast in the bone resorption stage. Conclusion : Through this study, F3570 extract revealed an outstanding inhibitory effect and signaling mechanisms in osteoclast differentiation induced by RANKL. These results suggest that F3570 is bone diseases associated with aging or osteoporosis caused by menopause in an aging society is expected to be a superior candidate for the treatment or the prevention

홍차 분말의 첨가량을 0~8%로 달리하여 제조한 스펀지 케이크의 물리·화학적 품질, 항산화 활성 및 소비자 기호도 를 비교하였다. 케이크의 pH와 높이는 홍차 분말의 첨가량 이 증가할수록 유의적으로 감소하였으며(p<0.05), 굽기 손 실률의 경우 홍차 분말의 함량이 증가함에 따라 유의적으로 증가하였다(p<0.05). 경도는 0~4% 시료 간에서는 유의적인 차이는 없었다(p>0.05). 한편 명도(L*)는 붉은색을 띄는 홍 차 분말 고유색의 영향으로 첨가량이 증가할수록 유의적으 로 감소하였고(p<0.05), 적색도(a*)와 유의적으로 증가하였 다. 황색도(b*)의 경우 증가하는 경향을 보였으나, 0~4%, 6~8% 시료 간에 유의적인 차이는 발견되지 않았다 (p>0.05). 항산화 활성을 나타내는 전자공여능과 ABTS 라 디칼 소거능 활성은 홍차분말을 첨가할수록 유의적으로 증가하는 경향이 나타났다(p<0.05). 소비자 기호도 결과 2~4% 첨가군이 색과 부드러움을 제외한 나머지 항목에서 대조군보다 높게 평가되었으며, 따라서 관능품질과 건강기 능성 효과 등을 고려할 때 소비자의 기호를 충족시키기 위한 최적 첨가비율은 2~4%가 가장 적합한 것으로 판단된 다.

This study analyzed fluctuations of ground water level of ground water wells developed in Seongsan watershed of Jeju Island until 2013 using MODFLOW, a numerical analysis model. Ground water level shows greater fluctuations from increase of pump capacity compared to the number of ground water wells. The development of ground water at the top of watershed was found to have direct influence on ground water level. Ground water wells developed until 2013 were used to continue pumping for 50 days, and ground water level of coastal region was reduced below 50% compared to the standard water level. In addition, the range of fluctuation of water level was large in the east coast region, which represents the direction of flow of ground water.

Jeju Island is the highest rain-prone area in Korea that possesses affluent water resources, but future climate changes are predicted to further increase vulnerabilities as resultant of increasing of extreme events and creating spatial-temporal imbalance in water resources. Therefore, this study aimed to provide basic information to establish a proper water resources management plan by evaluating the effects of climate change on water resources using climate change scenario. Direct runoff ratio for 15 years (2000~2014) was analyzed to be 11∼32% (average of 23%), and average direct runoff ratio for the next 86 years (2015∼2100) was found as 28%, showing an increase of about 22% compared to the present average direct runoff ratio (23%). To assess the effects of climate change on long-term runoff, monthly runoff variation of future Gangjeong watershed was analyzed by dividing three time periods as follows: Present (2000∼2030), Future 1 (2031∼2070) and Future 2 (2071∼ 2100). The estimated results showed that average monthly runoff increases in the future and the highest runoff is shown by Future 2. Extreme values has been expected to occur more frequently in the future as compared to the present.

In this study a hybrid energy dissipation device is developed by combining a steel slit damper and linear-slot friction dampers to be used for seismic retrofit of structures. The hybrid damper has an advantage in that friction dampers are activated for small earthquakes or strong wind while slit damper remains elastic, and both friction and slit dampers work simultaneously for strong earthquakes. Cyclic loading tests of the linear-slot friction, slit, and the combined hybrid dampers are carried out to evaluate their seismic energy dissipation capability.

A number of projects for development have been done continuously due to the increase of tourist in Jeju Island. However flood disaster countermeasure due to urbanization is not considered during this development projects. This study is to make basic process for the flood estimation in Han stream of Jeju Island. The variation of stream discharge due the every 5 years' land use change from 1980 to 2005. Data for flood events (rainfall and discharge) were collected for HEC-HMS model. Clark method was used for unit hydrograph analysis. For the estimation of Clark unit hydrograph parameters, Kraven II and Sabol’s empirical equations were applied. The peak discharge increased 9.9~33.67% and total discharge amount increased 12.53~30.21%. Also, time of concentration for peak discharge was reduced by 10 minutes for each event.

Rainfall-runoff model of Jeju Oedo Stream was used to compute the optimal unit hydrograph by HEC-HMS model that reflecting on watershed characteristics. Each rainfall event was comparatively analyzed with the actual flow measurement using Clark, Snyder and SCS synthetic methods for derived unit hydrograph. Subsequently, the null hypothesis was established as p-value for peak flow and peak time of each unit hydrograph by one-way ANOVA(Analysis of variance) was larger than significance level of 0.05. There was no significant difference in peak flow and peak time between different methods of unit hydrograph. As a result of comparing error rate with actual flow measurement data, Clark synthetic unit graph best reflected in Oedo Stream as compared to other methods, and error rate of Clark unit hydrograph was 0.02~1.93% and error rate at peak time was 0∼2.74%.

As occurrence of gradually increasing extreme temperature events in Jeju Island, a hybrid downscaling technique that simultaneously applies by dynamical method and statistical method has implemented on design rainfall in order to reduce flood damages from severe storms and typhoons.As a result of computation, Case 1 shows a strong tendency to excessively compute rainfall, which is continuously increasing. While Case 2 showed similar trend as Case 1, low design rainfall has computed by rainfall in A1B scenario. Based on the design rainfall computation method mainly used in Preventive Disaster System through Pre-disaster Effect Examination System and Basic Plan for River of Jeju Island which are considering climatic change for selecting 50-year and 100-year frequencies. Case 3 selecting for Jeju rain gage station and Case 1 for Seogwipo rain gage station. The results were different for each rain gage station because of difference in rainfall characteristics according to recent climatic change, and the risk of currently known design rainfall can be increased in near future.

In the mountain streams in Jeju Island, strong turbulence and roughness usually made it nearly impossible to utilize most of intrusive instrumentation for streamflow discharge measurements. Instead, a non-intrusive fixed electro-magnetic wave surface velocimetry (fixed EWSV: Kalesto) became alternatively popular in many representative streams to measure stream discharge seamlessly. Currently, Kalesto has shown noteworthy performance with little loss in flood discharge measurements and also has successfully provided discharge for every minute. However, Kalesto has been operated to regard its measured one-point velocity as the representative mean velocity for the given cross-section. Therefore, it could be highly possible to potentially encompass discharge measurements errors. In this study, we analyzed the difference between such Kalesto discharge measurements and other alternative concurrent discharge measurements such as Acoustic Doppler Current Profiler (ADCP) and mobile EWSV which were able to measure velocity in multi-points in the cross-section. Consequently, Kalesto discharge deviated from ADCP discharge in amount of 48% for relatively low flow, and more than 20% difference for high flow compared with mobile EWSV discharge measurements. These results indicated that the one-point velocity measured by Kalesto should be used as a cross-sectional mean velocity, rather it should be accounted for as an index-velocity in conjunction with directly measured cross-sectional mean velocity by using more reliable instrumentations. After inducing Kalesto Discharge Correction Coefficient (KDCC) that actually means relationship between index velocity and cross-sectional mean velocity, the corrected discharge from Kalesto was significantly improved. Therefore, we found that index velocity method should be applied to obtain better accuracy of discharge measurement in case of Kalesto operation.

최근 몇 년간 발생한 대형 화재사고의 경우 특수구조물에 대한 화재가 많으며 화재의 피해가 커진 경우 공통적으로 초기 진압에 어려움이 있었고, 적절한 방재작업을 수행할 장비의 부재가 원인으로 분석된 사례가 많았다. 이와 같은 특수구조물의 화재에 있어서 화재 진압을 위한 접근성이 용이하고 외부에서 화원으로 직접 방수 할 수 있는 기능을 갖춘 특수 방재장비의 도입 및 기술개발의 요구가 컸다. 이에 따라 우리나라의 산업형태에 따른 도시구조, 건축구조 등에 적합하고 기존의 유사 외국 기술이 갖고 있는 기술적 한계점을 극복한 “한국형 원격조정 파괴방수차”를 개발이 요구되었다. 본 연구에서는 목조건물, 문화재, 샌드위치 판넬 구조의 공장 및 창고, 컨테이너 Box, 항공기, 철도차량 등 특수목적 구조물의 화재 발생시 구조물의 외부를 국소파괴하고 내부로 방수노즐을 삽입 후 다량의 물 또는 폼 소화액을 방수하여 효과적인 초기 진압을 수행할 수 있는 소방장비에 대해 원격조정 굴절 붐 구조를 갖는 파괴방수차량의 국산화 설계기술, 제조기술, 하이브리드 타입 파괴방수 모듈의 설계/제작을 통한 굴절 붐 방수탑 통합 기술에 대해 연구하였다. 또한, IT기술을 접목한 노즐 모듈의 자동이송 및 아웃트리거 자동 셋팅 시스템 기술과 설계의 전 과정에 걸쳐 수치해석을 통한 설계검증 기술 그리고 시제품에 대해 자체 평가기준에 따른 성능평가에 대해 연구하였다.