This study was carried out to investigate insect community structure from different habitats in Baengnyeong island.We performed day and night collection at two different habitats (mountain, rural area) of Baengnyeong island from Mayto September in 2015. A total of 2,879 individuals of 404 species, 81 families belonging to 10 orders were collectedand identified. A dominant species was Idisia ornata Pascoe (Tenebrionidae) despite a very low percentage (6.04%) ofthe species among the catches. Results of independent t-test showed a significant high (p<0.001) of species richness onmountain. Also, seasonal results of ANOVA (Analysis of variance) were significantly influential with species abundanceand species richness. The result of NMDS analysis showed that the community structure of the insects from the mountainis different with rural area.
Oyster mushrooms are one of the most famous mushrooms for foods in Korea. ‘Santari’ developed on this study is P. pulmonarius of oyster mushroom species. ‘Santari’ is a new variety of oyster mushroom for the bottle culture. It was bred by mating with monokaryons isolated from ‘GMPO20404’ and ‘Hosan’. The optimum temperature for the mycelial growth was 26~29°C on PDA medium and that for the primordia formation and the growth of fruiting body of ‘Santari’ was 22°C and 20°C on sawdust media. It took 34 days to finish spawn running, 3 days to finish primordia formation, 3 days to finish fruitbody growth in the bottle culture. In the characteristics of fruit body, pilei were round type and brownish, stipe color was white and stipe shape was long and thick. The yield per bottle was 172g/1,100ml and was 43% higher than that of control variety(Hosan). As results of the physical properties of fruit body, springness, cohesive, gumminess and brittleness of stipe tissue were 87%, 82%, 193g and 16kg, respectively. The values of them are lower than those of control except cohesive.
Oyster mushrooms are one of the most famous mushrooms for foods in Korea. ‘Santari’ developed on this study is P. pulmonarius of oyster mushroom species. ‘Santari’ is a new variety of oyster mushroom for the bottle culture. It was bred by mating with monokaryons isolated from ‘GMPO20404’ and ‘Hosan’. The optimum temperature for the mycelial growth was 26~29°C on PDA medium and that for the primordia formation and the growth of fruiting body of ‘Santari’ was 22°C and 20°C on sawdust media. It took 34 days to finish spawn running, 3 days to finish primordia formation, 3 days to finish fruitbody growth in the bottle culture. In the characteristics of fruit body, pilei were round type and brownish, stipe color was white and stipe shape was long and thick. The yield per bottle was 172g/1,100ml and was 43% higher than that of control variety (Hosan). As results of the physical properties of fruit body, springness, cohesive, gumminess and brittleness of stipe tissue were 87%, 82%, 193g and 16kg, respectively. The values of them are lower than those of control except cohesive.
The purpose of the study was to determine the effect of joint mobilization with respiratory muscle taping on pulmonary function and chest expansion ability in patients with chronic stroke. Thirty subjects (n=30) were randomly divided into three groups: a joint mobilization group (n=10), a respiratory muscle-taping group (n=10), and a joint mobilization with respiratory muscle-taping group (n=10). Measurements for pulmonary function and chest expansion were performed to assess its effectiveness. A spirometer was used to measure the pulmonary function, and a tape measure was used to assess the chest expansion. In the joint mobilization group, peak expiratory flow (PEF) was increased. Forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and upper and lower chest expansion were also increased. All of variables of respiratory muscle-taping group also were increased. FEV1 and FVC were significantly different between joint mobilization with respiratory muscle-taping and respiratory muscle-taping group. The results of the present study suggest that mobilization with respiratory muscle taping increase the pulmonary function and chest expansion.
The purpose of this study was to examine the effects of abdominal hollowing with ultrasound biofeedback on multifidus and transversus abdominis/internal oblique muscle activities during lifting tasks. This study recruited 12 healthy men. All subjects received training on expiration without and with abdominal hollowing with ultrasound biofeedback. After receiving training for each condition, the thickness of transversus abdominis was measured using ultrasound, and then, electromyography activities of transversus abdominis/internal oblique and multifidus during lifting tasks were measured using a surface electromyography system. The differences in thickness of transversus abdominis and electromyography activities of transversus abdominis/ internal oblique and multifidus between the two conditions were analyzed using paired t-test. Thickness of transversus abdominis and transversus abdominis/internal oblique and multifidus activities were significantly increased during expiration with abdominal hollowing with ultrasound biofeedback compared to during expiration without abdominal hollowing (p < 0.05). These findings suggest that abdominal hollowing with ultrasound biofeedback is effective in increasing trunk stabilizer muscle activities during both static and dynamic tasks such as lifting.
Samples were collected between August 2016 and May 2017 at Sangju-si, Gyeongsangbuk- do, Jeju-do, and Uljin-gun, Gangwon-do. As a result, one genus and six species were newly recorded in Korea. The unrecorded indigenous genus was Borzia, and the six species were Anagnostidinema acutissimum, Komvophoron bourrellyi, Hydrocoleum stankovicii, Borzia trilocularis, Phormidium tinctorium and Pseudanabaena lohchoides. Phormidium tinctorium and Pseudanabaena lohchoides had been reported to inhabit freshwater, but in this study, these were found in brackish water. It had also been reported that B. trilocularis trichomes are composed of up to 8 cells, but in this study, a maximum of 10 cells was observed.
The freshwater algae were collected at reservoirs and small ponds in Gyeonggi-do and Jeju-do on June 2016. Four species of Korean previously unrecorded algae were collected in domestic fresh waters: Coelastrum rugosum, Cosmarium baccatum, Cosmarium norimbergense and Staurastrum connatum var. pseudoamericanum. The morphological characteristics of the four taxa identified in this study were showed mostly similar to the characteristics of the previously reported characteristics. However, Cosmarium baccatum was larger than the previously recorded species and Staurastrum connatum var. pseudoamericanum was wider than the previously recorded species.
목 적 : 가입한 (±)구면렌즈 굴절력에 대응하는 조절력의 변화를 비교하여 렌즈의 적응효과를 알아보았다. 방 법 : 평균연령 21.76±1.76세의 51명(남자 26명, 여자 25명)을 대상으로 하였다. 대상자들의 완전교정 된 눈 앞에 S+1.00 D, S+2.00 D, S+3.00 D, S-1.00 D, S-2.00 D, S-3.00 D를 순서대로 가입하여, 가입 직 후, 15분 후, 그리고 30분 후에 각 단안조절력을 측정하였다. 측정값들은 완전교정 상태의 조절력과 비교 하였고, 렌즈 가입 전·후 측정된 단안조절력의 변화값은 가입한 굴절력 값에 대한 상대 비율로 계산하였다. 결 과 : S+1.00 D, S+2.00 D, S+3.00 D 가입 후 조절력은 완전교정 조절력보다 모두 유의하게 (p<0.001) 증가되었지만, 가입된 렌즈굴절력의 약 55~68% 수준이었다. S-1.00 D, S-2.00 D, S-3.00 D 가입 후 조절력은 완전교정 조절력보다 모두 유의하게(p<0.001) 감소되었고, 가입된 굴절력의 약 72~105% 수준이었다. 결 론 : 조절변화를 목적으로 구면렌즈를 처방할 때, 임상전문가들은 렌즈적응으로 인해 더해준 구면굴절 력 값보다 조절변화량이 대부분 감소될 것이라는 점을 고려해야 한다.
A study on indigenous diatoms was carried out at 10 sites from May 2014 to December 2016 in marine and freshwater in Korea. Seventeen species of diatoms are new to Korea and they are divided into 3 classes, 4 subclasses, 10 orders, 14 families, and 16 genera. The nomenclatures, references, dimensions, specimens examined, local habitat, distribution in Korea, and photograph are reported here. Seventeen species found in marine, freshwater, and brackish water showed species-specific habitats.
The concept of „Sustainability‟ has become as major concern and it used by consumers and corporations to convey the concept of taking care of the environment. Environmental concern has led to sustainable consumption in a variety of product categories, such as electricity, textiles, apparel, food, and grocery products (Chan, 2001; Harrison, Newholm, & Shaw, 2005; Vermeir & Verbeke, 2006a, 2006b). Interest of the negative environmental impacts are rapidly increasing in present fashion business and consumer behavior has become a rising concern of the consumption and fashion supply chain to apply sustainable consumption (Birtwistle & Moore, 2007; Fineman, 2001). The environmental and social concern recognized in fashion industry from 1990‟s. However, the complexity of conceptual definition of sustainability and ecologically responsible consumer generates different and mistaken perception to consumer. In addition, in fashion industry, the terms of „eco-fashion‟, „environmentally friendly fashion‟,„green fashion‟, „ethical fashion‟, and „sustainable fashion‟ are frequently used interchangeably to describe the same concept. These interchangeable terminology is leading to confusion of the readers by the non-unified terminology (Choi et al., 2012). Also, consumers seem to have narrow scope and little understanding of sustainable fashion. In general, consumers focuses on environmental aspect not the wide-range of complexity of environment, social, and economical concern (Cervellon, Hjerth, Ricard, & Carey, 2010). The growing number of fashion brands are leveraging on green branding initiatives. Green marketing is increasing rapidly in corporate aspects and for a consumer perspective, global consumers are recognizing a personal accountability to take responsibility for social and environmental issues. Despite the fact many of individuals‟ willingness to purchase green products has increased in the last few years, however, there is limited studies suggest that purchase of green or sustainable products. Consumer research on sustainable fashion has mainly focused on consumer behaviors towards sustainable fashion products (SFPs); however, relevant studies that examined the whole process of the predicting proenvironmental behavior cross nationally value and the eWOM are still scarce. The purposes of research model are 1) to identify the determinants of eWOM intention on consumers' purchase intentions, 2) to examine the information adoption process as precursors of purchase intention of sustainable fashion, and 3) to testify different message types effects to information adoption process.
목적: 본 연구의 목적은 여러 산업의 선행연구들을 통하여 고객에게 제공되는 관계효익의 개념을 경제적 효익, 고객화효익, 사회적효익, 심리적효익으로 구분하여 전환장벽과 재구매의도에 미치는 영향에 대하여 확인하고자 하였다.
방법: 본 연구는 안경원에서 안경테, 안경렌즈, 콘택트렌즈를 구입하였던 347명의 고객을 대상으로 설문 조사하고 그 데이터를 분석하였다. 데이터 분석은 SPSS 18.0 통계프로그램을 이용하여 빈도분석, 요인분석, 회귀분석을 실시하여 안경원의 관계효익과 전환장벽, 재구매의도에 관한 연구 가설을 검증하였다.
결과: 첫째, 관계효익 중 심리적효익과 고객화효익이 전환장벽 형성에 긍정적인 영향을 미쳤다. 둘째, 관 계효익 중 세 요인, 즉 심리적효익, 경제적효익, 고객화효익이 재구매의도에 긍정적인 영향을 미치는 것으로 나타났다. 셋째, 전환장벽이 재구매의도에 긍정적인 영향을 미치는 것으로 나타났다.
결론: 본 연구에서는 안경원의 관계효익 중에서 심리적효익이 전환장벽과 재구매의도에 중요한 역할을 하 는 것과 관계효익의 네 요인 중 고객화효익이 전환장벽 형성과 재구매의도에 영향을 주는 것을 발견하였다. 경제적효익은 재구매의도 형성에만 영향을 미치므로 안경원 경영자는 안경사의 장기 근무를 위한 노력과 재 교육을 제공하여 고객관계관리를 통한 전환장벽 형성과 재구매의도를 높이려는 노력을 기울여야 할 것이다.
Alginate lyase from Streptomyces violaceoruber was purified by DEAE sephacel chromatography and SP sepharose chromatography. The specific activity of the purified enzyme was 14.6 units/mg protein, representing a 40.6-fold purification of the crude extract. The final preparation thus obtained showed a single band on Tricine-SDS polyacrylamide gel electrophoresis whose molecular weight was determined to be 23.3 kDa. The polyMG block of sodium alginate was hydrolyzed by the purified alginate lyase and then separated by activated carbon column chromatography and bio gel P-2 gel filtration. The main hydrolysates were composed of hetero type M/G-oligosaccharides with the degrees of polymerization (D.P.) being 6 and 8. To investigate the effects of hetero type M/Goligosaccharides from the sodium alginate on the growth of some intestinal bacteria, cells were cultivated individually on the modified-MRS medium containing D.P. 6 and 8 M/G-oligosaccharides. B. longumgrew 4.25-fold and 6.44-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides compared with those of standard MRS medium. In addition, B. bifidumgrew 3.3-fold and 5.4-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides. In conclusion, D.P. 8 was more effective than D.P. 6 hetero M/G-oligosaccharides as regards the growth of Bifidobacteriumspp. and Lactobacillus spp
Colorectal cancer is a major cause of morbidity and mortality that accounts for over 9% of all incidences of cancer. Additionally, colorectal cancer is widely recognized as an environmental disease related to ill-defined cultural, social and lifestyle factors including physical activity, obesity, cigarette smoking and heavy alcohol consumption. Accordingly, natural phytochemicals and extracts have attracted attention because of their beneficial biological effects. Coenzyme Q10 (CoQ10) is a common supplementary medicine applied to increase bioenergetic capacity in various diseases. Therefore, in this study, we investigated whether CoQ10 treatment has any inhibitory effects and its related cellular mechanisms in human colon cancer HCT116 cells. A MTT assay revealed that CoQ10 slightly decreased the proliferation of HCT116 cells; however, glutathione- and superoxide dismutase- activity were unchanged in response to CoQ10 treatment. A DCF-DA assay revealed that CoQ10 slightly increased ROS release of HCT116 cells. However, in a nitric oxide (NO) assay, CoQ10 significantly increased NO production in a dose-dependent manner. The results of western blot analysis revealed that the protein levels of Bax, p21 and p53 were increased, whereas the protein level of Bcl2 was decreased suggesting that the CoQ10-mediated inhibitory mechanism is associated with apoptotic signaling. Taken together, our findings indicate that CoQ10 has an inhibitory effect on the growth of colon cancer cells via NO production that is associated with regulation of factors involved in apoptotic signaling including Bax, Bcl2, p21 and p53.
We report on the dispersion state of partially reduced graphene oxide (PRGO) in organic solvents, namely methyl ethyl ketone, ethyl acetate, methylene chloride, toluene, and xylene, by controlling the carbon to oxygen (C/O) atomic ratio of the PRGOs. A two-phase solvent exchange method is also proposed to transfer PRGO from water to an aprotic solvent, such as methyl ethyl ketone. We achieve relatively good dispersion in aprotic and non-polar solvents by controlling the C/O atomic ratio of the PRGOs and applying the two-phase solvent exchange method. There is an increase in the glass transition temperatures with the dispersion of PRGOs into amorphous polymers, in particular a 4.4°C increase for poly(methyl methacrylate) and 3.0°C increase for polycarbonate. Good dispersion of PRGO in a nonpolar polymer, such as linear low density polyethylene, is also obtained.
Rubus coreanus is known to have diverse biological properties, such as free radical scavenging activity and antibacterial activity. In the present study, Rubus coreanus leaf and stem extract (RLSE) was used in boar semen preservation whether it has a beneficial effect on assisted reproductive technology (ART) in mammals. Boar spermatozoa were preserved in Beltsville thawing solution (BTS) in the presence of varying concentrations of RLSE (0-10 μg/mL). Sperm motility, sperm viability, and intracellular reactive oxygen species (ROS) levels were examined after 2 days of preservation. The percentage of total motile spermatozoa and progressive motile spermatozoa improved in the spermatozoa preserved with 0.5 μg/mL RLSE. Higher proportions of viable spermatozoa were seen in the presence of 0.5 and 1 μg/mL RLSE than in the control. Intracellular ROS levels decreased when the spermatozoa were preserved in BTS with 0.1–1 μg/mL RLSE. In order to examine the bacterial growth, E. coli was added to liquid semen diluted with antibiotics-free BTS in the presence or absence of RLSE. No anti-bacterial activity of RLSE against E. coli was observed during liquid semen preservation. Although there was no inhibition of E. coli growth, the addition of RLSE might help improve sperm motility and viability during boar semen preservation, suggesting it as a potential reagent for ART in mammals.
Anthriscus sylvestris (L.) Hoffm. is a perennial herb found widely distributed in various regions of Korea, Europe, and New Zealand. The root of A. sylvestris have been extensively used in the treatment for antitussive, antipyretic, cough remedy in Oriental medicine, but the physiologically active function of the leaf of A. sylvestris is as yet unknown. In this study, we investigated the anti-cancer activity and the mechanism of cell death of water extracts of leaf of Anthriscus sylvestris (WELAS), on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that WELAS treatment inhibited cell viability in a concentration- and time-dependent manner. In addition, the treatment of WELAS markedly induced apoptosis in FaDu cells, as determined by the viability assay, DAPI stain and FACS analysis. WELAS also increased the proteolytic cleavage of procaspase-3, -9 and PARP (poly(ADP-ribose) polymerase). In addition, exposure to WELAS decreased the expression of Bcl-2 (an anti-apoptotic factor), but increased the expression of Bax (a pro-apoptotic factor), suggesting that mitochondria-dependent apoptotic pathways are mediated in WELAS-induced apoptosis. Taken together, these results indicate that water extracts of leaf of A. sylvestris inhibits cell growth and induces apoptosis via the mitochondrial-dependent apoptotic pathway in FaDu human hypopharyngeal squamous carcinoma cells. Therefore, we propose that the water extracts of leaf of A. sylvestris is a novel chemotherapeutic drug, having growth inhibitory properties and induction of apoptosis in human oral cancer cells.
in vitro studies on salt torerance was carried out with Salix gracilistyla. Shoot growth of plants treated with NaCl showed statistically significances among different levels of concentration(0~200 mM) on 1/2MS medium. Plant growth was not grown at in higher concentration than 75 mM and plants were dead over 150 mM Nacl Browned leaf was observed from 14 days at higher concentration than then 75 mM NaCl, and chlorophyll content was dramatically decreased. Main roots and rootlet in same concentrates(75 mM) or high NaCl treatment were changed the color to black. The proline and sugar contents were dramatically increased in the higher than 75 mM NaCl treatments after 14 - 15 days. To determine NaCl and minerals in salt stressed plants, leaf, stem and root of in vitro plant were subjected to ICP analysis. Na content was increased with culture days in all three parts. These results suggest that S. gracilistyla can be used vegetative restoration in coastal wetlands reclaimed.