본 연구는 페튜니아(Petunia×hybrida)의 내건성 증진을 위 해 스트레스 메모리 기작을 활용한 프라이밍 처리의 효과를 구명 하고자 하였다. 건조-회복 과정을 통한 프라이밍 처리를 4회 반복 수행하였고, 두 가지 프라이밍 강도(Priming1:물 100mL, Priming2: 물 50mL 관수 후 건조)를 적용한 후 5일 동안 건조스 트레스 조건에서 내성 증진 효과를 관찰하였다. 프라이밍 강도가 높은 Priming2 처리구에서 대조군에 비해 수명이 약 25시간 연장되었다. 이는 페튜니아가 스트레스 메모리 기작을 통해 건조 스트레스에 대한 내성을 높일 수 있음을 보여준다. 본 연구는 정원 식물의 환경 스트레스 내성을 증진시키기 위한 프라이밍 기술의 유용성을 확인하였으며, 향후 다양한 환경 스트레스 조건 에서의 프라이밍 효과를 평가함으로써 화훼식물의 내건성 증진 에 대한 실용적 기술 개발에 기여할 수 있을 것으로 기대된다.

Background: The ability of adeno-associated viruses (AAVs) to transduce various cell types with minimal immune responses renders them prominent vectors for gene editing (GE), with different AAV serotypes exhibiting distinct transduction efficiencies due to their specific cellular tropism. However, detailed molecular processes of AAV infection and penetration, as well as the optimal serotype for specific purposes, remain poorly understood. Porcine models are widely used in research benefitting both human and livestock due to anatomical and physiological similarities to humans. Methods: Transduction efficiencies of 18 AAV serotypes (AAV1–9, 6.2, rh10, DJ, DJ/8, PHP.eB, PHP.S, 2-retro, 2-QuadYF, and 2.7m8) were evaluated in immortalized porcine lung epithelial cells (pLCsImt) and pulmonary alveolar macrophages 3D4/31 (PAMs 3D4/31). Results: We found AAV2, DJ, and 2.7m8 to be the most effective in both cell types. The highest enhanced green fluorescent protein expression of 52.46 ± 2.4% in pLCsImt and 64.08 ± 2.4% in PAMs 3D4/31 was observed for AAV2, while negligible transduction was observed for AAV4, rh10, DJ, PHP.eB, PHP.S, and 2-retro. AAV-DJ showed superior transduction efficiency in PK-15, as compared to AAV2 and 2.7m8. Results emphasize the cell type-specific nature of AAV serotype transduction efficiencies. Notably, AAV2 was most effective in both lung and macrophage cells, whereas AAV-DJ was more effective in renal cells. Conclusions: Our findings suggest that AAV2 was identified as the most efficient serotype for transducing pLCsImt and PAMs 3D4/31, compare to the PK-15 cells. Understanding cell type-specific preferences of AAV serotypes offer crucial insight for tailoring AAV vectors to specific tissue and optimizing genome editing strategies, with potential implications for the advancement of personalized medicine and development of treatments for human and livestock.

Clove (Syzygium aromaticum) is a highly valued medicinal plant native to Aisa. Widely used as a spice, renowned for its medicinal properties, particularly in Ayurveda and traditional Chinese medicine. In this study, clove bud extract (CBE) was prepared at different ethanol concentrations of 50%, 80%, and 90%, respectively. The antioxidant activity of the CBE was evaluated through DPPH, polyphenol, and reducing power assays, revealing its strong antioxidant potential, with 90% ethanol being the most effective extract. HPLC analysis identified eugenol (8.7 mg/g) as the major active compound, known to possess anti-inflammatory and antioxidant properties. Given the role of oxidative stress and inflammation in atopic dermatitis (AD), the therapeutic potential of CBE was explored using a 1-chloro-2, 4-dinitrobenzene (DNCB)-induced AD mouse model. Five-week-old BALB/c mice were induced with AD by topical application of DNCB. CBE was administered topically to the affected skin (back and ear) areas for 4 weeks. The treatment of CBE significantly reduced the severity of clinical dermatitis, decreased epidermal thickness, and lowered mast cell and eosinophil infiltration in skin tissue, as observed through hematoxylin eosin staining and toluidine blue staining. The results demonstrated CBE as a promising therapeutic agent for managing AD through its regulation of skin inflammation and oxidative stress, making it a potential candidate for future treatments of inflammatory skin disorders.

Background: The increasing prevalence of smartphone use has been associated with musculoskeletal pain; however, the specific roles of demographic factors, smartphone usage time, posture, contents and state of addiction on pain in the upper-body regions remain unclear. Objects: This study investigated the influence of smartphone usage characteristics, including age, occupation, visual condition, duration, content, and posture, as well as smartphone addiction, on musculoskeletal pain in upper-body regions. This study aimed to comprehensively elucidate the factors contributing to the pain associated with smartphone use. Methods: A cross-sectional survey was conducted with 316 participants aged 20–59 years. Data on personal characteristics, smartphone use patterns, state of addiction (measured using the Smartphone Addiction Scale-Short Version), and musculoskeletal discomfort (Cornell Musculoskeletal Discomfort Questionnaire and Cornell Hand Discomfort Questionnaire) were collected. Binary logistic regression analysis identified significant predictors of pain in different body regions. Results: Younger age (20–30 years), being housewives or students, and vision impairment (shortsightedness) significantly increased the likelihood of neck, shoulder, and hand pain. Prolonged smartphone use (7–10 hours daily) and gaming were strongly associated with elevated pain risk, whereas moderate usage (1–4 hours daily) may be protect against lumbar pain. Non-neutral postures, especially side lying, have emerged as critical risk factors, with left-side lying linked to hand pain and right-side lying linked to upper back pain. Smartphone addiction consistently predicted pain across all regions by amplifying physical strain through prolonged engagement and poor posture. Conclusion: This study highlighted the multifactorial nature of smartphone-related musculoskeletal pain, emphasizing the roles of demographic characteristics, usage patterns, and addiction. These findings provide a foundation for developing tailored ergonomic and behavioral interventions to mitigate pain risks, particularly in high-use populations. Future research should validate these findings through longitudinal studies and objective measures.

목적 : 본 연구에서는 노래의 템포(bpm) 및 데시벨(dB)에 따른 동공 크기 및 동적 조절 변화를 비교하고, 빛의 유무에 따른 차이도 비교해 보고자 한다. 방법 : 연구 대상은 안질환이나 시각 장애가 없는 성인 20명(남자 10명, 여자 10명, 평균연령 24.25±3.99세) 이었다. 완전 교정 상태에서 명소시에서 6 m 거리의 시표로, 암소시에는 무한대 거리로 측정하였다. 두 조건 모두 헤드셋을 착용하여 네 가지 음악 드럼(D), 메탈(M), 외국 팝(F), 클래식(C)을 들었을 때 동공 크기와 조절 반응의 변화를 각각 40 dB 미만, 60 dB 미만, 80 dB 이상으로 측정하여 비교하였다. 결과 : bpm 및 dB에 따른 자극이 있을 때, 유의한 차이를 보였다(p<0.010). 두 조건 모두 동일한 dB에서 bpm 이 달라졌을 때 자극량의 차이는 있었으나, bpm 변화에 따른 동공 크기와 동적 조절 변화량은 유의한 차이가 없었 다. 동공 크기와 동적 조절 변화량을 조도에 따라 비교했을 때, D 장르의 40 dB(p<0.050)과 F 장르의 60 dB (p<0.050)에서 동공 크기 변화량의 차이가 있었다. 조도가 없을 때 동적 조절 변화량이 더 증가하여, bpm과 dB에 따른 모든 그룹에서 유의한 차이가 나타났다(p<0.050). 결론 : 본 연구 결과, 각 노래의 bpm과 dB에 따른 동공 크기와 동적 조절 변화는 자극의 유무에 따라 영향을 받는 것으로 확인되었다. 특히, 암소시에서는 동적 조절 변화에 더 큰 영향을 미쳤으며, 명소시에서는 동공크기 변 화가 컸다.

Background: This study was conducted to increase meat production by 30% compared to the present by selecting a giant cow over 1,000 kg and applying biotechnologies. Methods: After OPU from 1,100 kg of giant cow, the calves were produced in Hanwoo surrogate mothers. Among 23 calves six male heads were selected, three heads of them were chosen as candidate sires, and the rest three heads were raised for 30 months for performance test. The semen of three candidate sires from the age of 22 months were collected and frozen, and the calves were produced by artificial insemination. The calf was raised to the age of 30 to 33 months and performed a progeny test. Results: The average birth weight of 23 calves born by transferring giant cow-derived embryos was 42.8 kg, and the average weight of carcass from three bulls was 615.3 kg in the performance test. In the progeny test, the average birth weight of calves born after artificial insemination of semen from giant cow-derived candidate sires was 41 kg, and the average weight of carcass after raised to the age of 30 to 33 months was 562.7 kg. As a result of performance and progeny tests, it is 148 and 96 kg higher in giant cow-derived beef cattle than the average carcass (467 kg) from general Hanwoo cattle, respectively. Conclusions: This study will have laid a great foundation for the future improvement of the Korean beef industry.

This study was conducted to investigate changes in the productivity of Italian ryegrass seeds according to the timing of harvest after heading in the southern region. The Italian ryegrass variety ‘Greencall’ was sown in Jinju, Gyeongsangnam-do, in the fall of 2022. Four harvest timings were tested (30, 40, 50, and 60 days after heading), with a randomized complete block design and three replicates. Sowing in the test plots took place on October 10, 2022, and harvesting was carried out from 30 days after heading on May 18 to 60 days. The plant height was the longest (99.1 cm) in the plot harvested 30 days after heading and decreased as the harvest was delayed. No significant differences were observed among treatments in terms of lodging resistance, disease resistance, and cold resistance. However, lodging severity increased over time after heading (7∼9). Disease incidence was also higher in plots harvested 50 and 60 days after heading. The length of the spike was shortest (38.76 cm) in the plot harvested 50 days after heading, and the number of seeds per spike was the lowest (42 seeds/spike) in the plot harvested 60 days after heading. The dry matter content of seeds increased with the delay in harvest, while dry matter yield decreased, with the lowest yield observed in the plot harvested 40 days after heading (3,031 kg/ha, p<0.05). The dry matter content of seed straw was highest at 75.73% in the plot harvested 50 days after heading and dropped to 34.99% 60 days after heading due to rainfall. The dry matter productivity of the seed straw was the lowest in the plot harvested 40 days after heading. The feed value of the seed straw also decreased with delayed harvest, with an average RFV (Relative Feed Value) of 91. In conclusion, the optimal harvest timing for fall-sown Italian ryegrass intended for seed production in the southern region appears to be 30 days after heading.

꼬리말발도리(Deutzia paniculata Nakai)와 가침박달 (Exochorda serratifolia S.Moore)은 각각 5~6월과 4~5월에 흰색 꽃이 피는 화목관목으로 한국의 희귀식물이며, 관상가치가 높아 원예용으로 개발할 가치가 있다. 본 연구에서는 꼬리말발 도리와 가침박달의 종자를 이용한 번식체계를 확립하고자 플러 그 육묘에서 플러그 트레이의 셀 크기, 배지조성, 시비수준에 따른 유묘의 생육을 조사하였다. 꼬리말발도리는 플러그 트레이 의 셀 크기가 작아질수록 유묘의 생육이 불량하였고, 105구의 셀 크기에서 초장, 엽장과 엽폭, 줄기와 뿌리의 생체중 등 생육 이 가장 양호하였다. 또한 배지의 조성은 피트모스와 펄라이트 를 20:80로 혼합한 처리에서 줄기와 뿌리의 생육이 가장 우수하 였고, 펄라이트 단용인 0:100 처리에서 유묘의 생육이 가장 불 량하였다. 가침박달은 피트모스와 펄라이트를 100:0과 75:25 로 혼합한 배지에서 유묘의 생육이 가장 좋았고, 펄라이트 단 용인 0:100 배지에서 유묘의 줄기뿐만 아니라 뿌리의 생육 도 가장 불량하였다. 그리고, 육묘 기간동안 시비수준이 증가 함에 따라 유묘의 줄기 생육이 양호하였으며, 엽록소의 함량도 증가하였다. 따라서 가침박달은 플러그 트레이 당 Hyponex (N:P:K=20:20:20) 6.24.g을 관주하는 것이 고품질 묘를 생산 하는데 가장 효과적이었다. 따라서 꼬리말발도리는 105공 플러 그 트레이에 피트모스와 펄라이트의 20:80 비율의 배지를 사용 하는 것이, 가침박달은 피트모스와 펄라이트의 100:0과 75:25 비율의 배지에서 육묘기간동안 Hyponex 6.24g를 시비하는 것이 유묘 생육에 가장 적합하였다.

For centuries, humans have leveraged the health-promoting properties of plants for our well-being. While research has been conducted on numerous medicinal plants, the specific benefits of many species remain underexplored. Eupatorium Japonicum (EJ), a member of the Asteraceae family, has historically been consumed in Japan, South Korea, China, and Vietnam for its traditional use in soothing digestive issues. This study aimed to explore the radical scavenging and antiinflammatory efficacy of EJ extract using RAW 264.7 cells. The radical-scavenging effects were assessed using the DPPH and ABTS assays, where an anti-oxidative molecule in the test sample will react with a stable free radical in DPPH and ABTS causing discoloration. The anti-inflammatory efficacy was assessed using the nitric oxide (NO) assay in LPS-induced RAW 264.7 cells, where the amount of NO produced in response to infection was measured using Griess reagent. Reversetranscriptase polymerase chain reaction (RT-PCR) and real-time PCR were executed to confirm the anti-inflammatory activity by measuring the RNA levels of pro-inflammatory cytokines. The DPPH and ABTS assays revealed that EJ extract decreased oxidation in a concentration-dependent manner (7.8-1,000 μg/mL) compared to ascorbic acid and Trolox respectively. EJ extract significantly reduced NO production concentration independently. Furthermore, EJ extract showed no cytotoxic effects as determined through the MTT assay. RT-PCR and real-time PCR analyses revealed inhibition of mRNA expression of pro-inflammatory cytokines (iNOS, COX-2, TNF-α, and IL-6). Western blotting demonstrated EJ’s anti-inflammatory activity by reducing protein levels of iNOS, COX-2, TNF-α, and IL-6. These findings suggest that EJ extract exhibits anti-inflammatory activities and can be further evaluated in the future.

This study investigated the relationship between changes in language learning beliefs and English proficiency among 41 Korean university students who participated in a short-term English program. Participants’ beliefs were assessed using the Beliefs About Language Learning Inventory (BALLI), and their proficiency was measured using the Test of English for International Communication (TOEIC). Frequency analysis, descriptive statistics, paired-sample t-tests, and correlation analysis were employed to analyze the data. The study found significant improvements in both listening and reading scores, and changes in beliefs varied with proficiency gains. Students with higher proficiency gains demonstrated improved confidence and self-efficacy, and decreased instrumental motivation, whereas those with lower gains exhibited minimal changes in beliefs. Correlation analysis revealed that belief shifts, such as reduced selfconsciousness and increased integrative motivation, were positively related to proficiency gains. These findings suggest the dynamic nature of learners’ beliefs and their potential impact on language learning outcomes, highlighting the importance of addressing belief systems in English language education.

Background: Lower limb strength is crucial for stability and functional movement, such as walking, running, squatting, and balance, with the gluteus maximus (Gmax) being pivotal. Squat exercises are commonly used to strengthen the Gmax; however, the impact of ankle position on muscle activation during squats is not well understood. Objects: This study examined Gmax and lower limb muscle activation patterns in three ankle positions during squats, aiming to optimize rehabilitation strategies and enhance exercise prescriptions. Methods: Surface electromyography recorded the activation levels of the Gmax, vastus medialis oblique (VMO), vastus lateralis oblique (VLO), and biceps femoris (BF) across three ankle positions: neutral (NEU), dorsiflexion (DF), and plantarflexion (PF). A repeated-measures design was employed, involving 30 healthy adults (26 males and 4 females) aged 18–30 years. Muscle activation patterns were statistically analyzed to identify significant variations across these conditions, with the significance level set at p < 0.05. Results: During squats, DF of the ankle joint significantly increased Gmax activation compared with PF and NEU positions, indicating that an ankle position closer to DF may enhance hip extension. In contrast, PF was associated with heightened activation of the VMO and VLO, suggesting that this position may be beneficial for exercises focusing on knee stability. No significant changes were observed in the BF activation across the ankle positions, indicative of its limited involvement in response to variations in ankle positioning. Conclusion: These results underscore the importance of ankle joint positioning in modulating lower-limb muscle engagement during squatting. Ankle DF may be recommended to maximize Gmax activation, which is beneficial for hip-focused strengthening, whereas PF may supports knee stability by targeting quadriceps activation. This study provides evidence for adjusting ankle positioning during squat exercises to optimize specific rehabilitation and performance outcomes.

정원과 대조구 간 열 환경 요소의 차이와 정원 구성 요소 간 열 환경과 열 스트레스 지수의 차이에 대한 결과는 다음과 같다. 정원과 대조구 간 열 환경 요소의 시계열 변화에서 대기 온도의 차이는 0.8°C에서 2.4°C까지 나타났고, 흑구 온도는 4.4°C까 지 나타났으며, WBGT는 1.6°C 차이가 나타났다. 대조구에 비 해 정원의 열 환경이 모든 온도에서 낮게 나타난 것이다. 특히, 대조구와 정원의 온도 증가 폭을 비교해 보면, 정원에서 모두 온도의 완만한 증가 폭을 확인할 수 있었다. 정원이 급격한 온도 차이는 만들어낼 수 없으나, 소규모 녹지를 활용한 지속적인 온도 조절에 효과를 보일 수 있겠다. 이용객이 느끼는 열 스트레 스 지수도 정원에서 낮게 나타나 정원이 열 쾌적감 증진 역할을 할 수 있다고 판단된다. 정원 구성 요소에 따른 열 환경 변화를 비교하였을 때, 목재 소재의 시설물이 표면 온도가 높았고, 녹지 에 의해서 생긴 그늘이 온도 차이를 줄일 수 있는 요소가 되었다. 실외 미기후를 대상으로, 정원 입지 환경 중에 하나인 건물에 의한 그늘과 녹지 그늘이 열 스트레스 지수인 UTCI에서 동일하 게 ‘moderate’ 수준을 나타냈다. 즉, 건물 그늘의 일사 차단 효과로 인해 열 쾌적감이 증진되는데, 건물이 없는 소규모 녹지 에서도 교목과 초지 식재로 열 쾌적감 증진에 효과를 높일 수 있다. 이와 같이 정원 구성 요소에 미기후 조절 효과에 관련이 있는 요소를 선정하였을 때, 건물→수목→주변 포장재→정원 포 장재→시설물의 순서로 정원 입지 선정과 구성 요소 선택에 활 용할 수 있는 가이드를 마련할 수 있을 것이다.

Afoxolaner is an insecticide and acaricide that belongs to the isoxazoline chemical compound group. it has been used as an active pharmaceutical ingrdient in veterinary medicine to treat fleas and ticks in dogs. When patents expire between 2026 and 2066, it is expected that many products will be applied for approval as generic products, and reserch to establish accurate quality control methods must be conducted and managed. HPLC method was developed for the quantitative and qualitative of afoxolaner in veterinary medicinal products. The separation of active constituents for afoxolaner was achieved on a RP18 (4.6 x 150 mm, 5 μm) column using Water : Acetonitrile : MeOH (25:30:45 v/v/v) as mobile phase, with UV detection at 245 nm. The method was validated for specificity, linearity, accuracy and precision. All calibration curves showed good linearity (R2 of 0.999) within the concentration ranges (12.5 to 400 μg/mL). For accuracy, the recovery rate was calculated by spiking three concentrations of standard into the sample blank. The recovery rate was calculated to be 99.70~100.58%. Precision was measured 9 times repeatedly through intra-day, inter-day tests using standard. It showed excellent precision by satisfying the relative standard deviation of less than 2% both intra-day and inter-day. Limit of detection (LOD) and limit of quantitation (LOQ) were 2.0 μg/mL and 6.1 μg/mL, respectively. This method was successfully applied to analyzing afoxolaner drugs distributed in Korea. The HPLC method described in this study is accurate and reproducible and could be applied for the analysis of veterinary drugs of afoxolaner.

Background: Stroke patients commonly experience functional declines in balance and gait due to decreased muscle strength and coordination issues caused by brain damage. Through repetitive training, robot-assisted gait training (RAGT) can aid in promoting neuroplasticity in stroke patients and help them acquire effective gait patterns. Additionally, convalescent rehabilitation hospitals help to ensure rapid recovery through intensive rehabilitation training. Objects: This study investigated the effects of RAGT frequency on gait and balance recovery in stroke patients in convalescent rehabilitation hospitals, providing data to optimize rehabilitation efficiency, enhance functional recovery, and support the development of personalized strategies to ensure safer and more rapid returns to daily life. Methods: This study compared the frequency of RAGT by analyzing a group receiving two units of RAGT per day for 5 days per week with a group receiving two units of RAGT per week as part of a comprehensive rehabilitation program, totaling 16 units daily, in a convalescent rehabilitation hospital. Results: In the 10-minute walking test, statistical significance was observed both within and between groups, whereas the Functional Ambulation Category, Fugl-Meyer Assessment–lower extremities, Berg Balance Scale, and timed up-and-go tests showed significance only within groups. Conclusion: End-effector RAGT and traditional gait training significantly improve gait ability, balance, and lower limb function in stroke patients.

Background: Cisplatin, a chemotherapeutic agent often causes nephrotoxic side effects. Lipopolysaccharide (LPS) is known to induce pro-inflammatory responses, often leading to septic renal injury. We hypothesized that the combination of cisplatin and LPS would amplify renal injury, thereby improving a renal injury model. Therefore, we administered both agents to mice and evaluated renal injury indicators. Methods: Eight-week-old male C57BL/6 mice were injected with cisplatin (8, 10, or 12 mg/kg) and LPS (5 mg/kg) on days 1 and 4 following of each week. Mice were euthanized at specific time points to assess renal injury. Body weight, renal weight, area, and BUN levels were measured to evaluate renal damage. Additionally, hematoxylin and eosin (H&E) and Masson’s trichrome (MT) staining were performed to assess histological changes. Results: The combination of cisplatin and LPS significantly reduced body and renal weight compared to cisplatin alone. A high dose of cisplatin (12 mg/kg) resulted in a 50% mortality, while, lower doses (8 and 10 mg/kg) showed 100% survival. Significant renal injury was observed in the 10 mg/kg cisplatin group administered for two weeks. In the 8 mg/kg cisplatin group, no changes were observed after two weeks, but renal damage appeared after four weeks. Histological evaluations in the 10 mg/kg cisplatin group administered for two weeks showed renal injury features, including tubular damage and fibrosis. Conclusions: Administering cisplatin (10 mg/kg) with LPS for two weeks or cisplatin (8 mg/kg) with LPS for four weeks resulted in a distinct renal injury, effectively establishing a renal injury mouse model.

A protruding mass was identified in the papilla of the right kidney of a 10-week-old male Sprague-Dawley rat. Microscopically, the neoplastic tissues were consisted of epithelial elements, where basophilic neoplastic cells displayed a high nucleus-to-cytoplasm ratio and formed tubular growth patterns characterized by small, elongated, or convoluted tubules. Blastemal elements were often arranged in aggregates or nests, composed of tightly packed basophilic polygonal to spindloid primitive cells. The surrounding interstitial tissue appeared loose and myxomatous. Based on these histological features, the diagnosis was nephroblastoma. Nephroblastoma is considered as an embryonic tumor originated from metanephric blastemal elements in the renal cortex and typically displays characteristic triphasic patterns. Also, this tumor seldom arises from or remains localized to the renal pelvis. To our literature review, this is the first nephroblastoma occurred at renal papilla in a rat.

개회나무(Syringa reticulata)는 수수꽃다리속 물푸레나무과 에 속하는 낙엽활엽교목으로 관상 및 약용 등으로 사용되는 가 치가 높은 식물이다. 본 연구는 개회나무 종자의 휴면타파와 발아특성을 구명하여 대량증식 체계를 확립하고자 하였다. 이에 따라 종자내외부특성, 수분흡수률, 변온처리(25/15, 20/10, 15/6, 5℃), 저온층적처리(0, 4, 8, 12주), 생장조절제 농도 (GA3 0, 10, 100, 1000mg·L-1)처리하여 확인하였다. 그 결과 배는 완전히 성숙되어 있는 형태였으며, 수분흡수률은 초기대비 약 39.2% 증가하였다 변온 처리조건에서는 15/6℃ 조건에서 발아율이 69.2%로 가장 높았으며, 0주 층적처리에서 가장 높은 발아율을 나타낸 것으로 보아 변온의 5℃ 처리의 결과와 동일한 양상으로 저온은 발아에 큰 영향을 미치지 않았다. 생장조절제 처리에서는 상대적으로 GA3 농도가 높은 100, 1000mg·L-1 처리에서 발아률이 통계적으로 높게 나타났다. 위 결과를 보아 개 회나무는 생리적 휴면을 가지고 있으며, 세부적으로 non-deep PD라고 판단된다.

Baicalin, a flavonoid isolated from Scutellaria baicalensis, has anti-inflammatory, antioxidant, and neuroprotective effects. Glutamate is a major neurotransmitter that plays an important role in brain function, but excessive release of glutamate causes excitotoxicity and damages cells. We investigated the neuroprotective effects of baicalin in glutamate-exposed neurons. The mouse hippocampal neuronal cell line (HT22) was cultured in a general manner, glutamate and/or baicalin were treated on the cells. Baicalin was administered 1 hr before glutamate treatment. Cells were collected 24 hr after glutamate, and cell viability was measured using MTT assay. Reactive oxygen species (ROS) and lipid peroxidation (LPO) assays were performed to measure oxidative stress. Glutamate reduced cell viability in a dose- and time-dependent manner. MTT assay showed that baicalin treatment ameliorated the decrease in cell viability due to glutamate toxicity. The effect of baicalin is dose-dependent. Glutamate caused severe nerve damage, including condensation of the cell shape, loss of dendrites and axons. However, baicalin treatment attenuated these morphological changes, and the effect of baicalin was dose-dependent. ROS and LPO analyses showed that glutamate increases oxidative stress, and baicalin attenuates this change due to glutamate toxicity. The effect of baicalin on these results was dose-dependent. We confirmed that baicalin performs an antioxidant function against glutamate toxicity in neurons. In conclusion, these results suggest that baicalin exerts neuroprotective effects on damaged neurons through antioxidant activity.

Background: Limited ankle dorsiflexion (DF) range of motion (ROM) is associated with mechanical dysfunctions and chronic ankle instability. Uphill treadmill walking exercise (UTWE) has the potential to improve ankle mobility and function. Objects: This study aimed to examine the immediate effects of a 15° UTWE on DF ROM and torque generation in patients with limited DF. Methods: The study included 20 adults, comprising 10 males and 10 females, with a mean age 28 years and a passive DF range of 5°–12°. After baseline assessments, participants completed 30 minutes of UTWE on a 15° incline treadmill, followed by post-assessments. DF ROM was evaluated during the stance phase of gait, and in both open-kinematic-chain (OKC) and closed-kinematic-chain (CKC) conditions using a goniometer and Image J software. DF and plantar flexion (PF) peak torque were measured with a Biodex Dynamometer. Results: Post intervention, maximum DF ROM during the stance phase of gait increased significantly from a mean of 8.54° ± 3.97° to 11.03° ± 4.41°. The DF ROM in the OKC and CKC conditions increased significantly from a mean of 8.90° ± 0.62° and 21.55° ± 0.72° to 18.00° ± 1.13° and 28.20° ± 1.00°, respectively (p < 0.0001). DF peak torque increased from 16.20 ± 1.28 N/m to 21.52 ± 1.39 N/m, and PF peak torque increased from 25.26 ± 2.51 N/m to 44.22 ± 4.20 N/m (p < 0.0002). Conclusion: UTWE significantly enhanced DF ROM and ankle torque, indicating that it may be an effective intervention for improving ankle function and preventing injury in clinical and rehabilitation settings.

Silage inoculants, which include beneficial microorganisms like lactic acid bacteria (LAB), play a vital role in modern silage production by enhancing fermentation quality. This study evaluated the effectiveness of various commercial inoculants on the fermentation dynamics of Italian ryegrass silage over 45 days. The treatments included a control group and five inoculant formulations: T1 (Lactiplantibacillus plantarum), T2 (Lactiplantibacillus plantarum and Pediococcus pentosaceus), T3 (Lactiplantibacillus plantarum and Pediococcus pentosaceus and Lactiplantibacillus buchneri), T4 (Lactiplantibacillus plantarum and Lactiplantibacillus acidophilus and Lactiplantibacillus bulgaricus), and T5 (Lactiplantibacillus plantarum and Pediococcus pentosaceus and Enterococcus faecium). After 45 days, all treatment groups exhibited significantly higher crude protein (CP) content compared to the control group (80.64 g/kg dry matter (DM), p<0.05). Treatments T2 and T5, which incorporated combinations of Lactiplantibacillus plantarum, Pediococcus pentosaceus and Enterococcus faecium, showed higher CP contents at 105.53 and 107.05 g/kg DM, respectively. The inoculated silages also demonstrated a rapid pH reduction within the early days, with Lactiplantibacillus plantarum in T1 reducing the pH to 4.0 within four days. Additionally, inoculated treatments had significantly higher lactic acid levels than the control (67.96 g/kg DM, p<0.05), and T3 (Lactiplantibacillus buchneri) produced higher acetic acid levels (16.07 g/kg DM, p<0.05) than other inoculants. The control group also had a notably higher ammonia nitrogen content. In conclusion, while single-strain inoculants like Lactiplantibacillus plantarum are effective for rapid acidification, the use of combined bacterial strains can further enhance silage quality by improving lactic acid fermentation and nutrient preservation, particularly in treatments like Lactiplantibacillus plantarum and Pediococcus pentosaceus and Lactiplantibacillus buchneri and Enterococcus faecium.