본 연구는 아이스플랜트의 기능성 소금 대체제로서의 가능성을 조사하고자 천연 및 정제소금과의 NaCl, Dpinitol, 총 페놀, 총 플라보노이드 및 DPPH 라디칼 소거능에 대한 기초자료를 비교 조사하였다. 아이스플랜트 동 결건조분말의 NaCl 함량을 분석한 결과 19%이었고 Dpinitol의 함량은 777 mg/100 g으로 천일염 및 정제염에 비해 NaCl 함량은 유의적으로 낮으나 D-pinitol을 함유하고 있어 건강기능성에는 도움이 될 것으로 사료된다. 또한, 아이스플랜트 동결건조분말의 총 페놀 및 플라보노이드 함량은 각각 115 mg/100 g 및 985 mg/100 g을 함유 하고 있어 DPPH라디칼 소거능의 항산화효과를 갖는 것으로 나타났다. 따라서 아이스플랜트 분말의 bioactive compound을 함유한 대체 소금 천연소재로서 가능성을 확인한 기초 연구를 제공하였다.

농촌진흥청 국립원예특작과학원에서는 2017년 빅립 형태의 분홍색계 팔레놉시스 ‘Lovely Angel’을 개발하였다. 2009년 백색 팔레놉시스 P. ‘Timonthy’와 빅립을 가지는 분홍색 P. World Class ‘Big Foot’을 모본과 부본으로 교배하였다. 2013년 생육이 우수하고 화색, 화형, 꽃대수 등 개화 특성이 우수한 ‘09056-12’를 개체선발하여 기내 화경배양을 통해 증식하였다. 2014년부터 2017년에거쳐 1차, 2차 특성검정을 통해 품종의 안정성과 균일성을 확인하여 ‘Lovely Angel’을 육성하였다. 이 품종은 밝고 선명한 보라빛 분홍색(RHS, PVG81C)을 띄며, 줄 무늬를 가지고 있고 설판이 큰 빅립 형태가 특징적이다. 안아피는 형태의 꽃은 길이와 폭이 각각 6.1, 5.6cm이며, 1개의 꽃대에 20.6개의 소화가 착생하여 꽃 수가 비교적 많이 달리고, 꽃대 길이는 31.8cm 이다. ‘Lovely Angel’는 생육시 잎은 반하수로 자라며 길이는 18.7cm, 폭은 6.8cm이다. 신품종인 ‘Lovely Angel’은 소형 분화용으로 이용 가능할 것이다.

‘Field Green’은 농촌진흥청 국립원예특작과학원에서 2012년에 개발한 국화 신품종이다. 2008년 황색 홑꽃인 ‘Laque Roicks’를 모본으로 하고 흰녹병저항성이며 연두색 홑꽃인 ‘Frill Green’을 부본으로 인공교배를 실시하였다. 실생개체중에서 녹색이며 잘 자라고 꽃모양이 안정적인 개체를 2009년에 선발하였다. 그리고 2010년부터 2012년까지 3년동안 촉성재배와 억제재배, 자연재배을 통해 특성검정을 실시하고 최종 선발을 하였다. ‘Field Green’ 은 자연일장조건에서 10월 26일에 개화하는 품종으로 일장조절을 통해 연중재배가 가능하였다. ‘Field Green’ 품종은 밝은 녹색의 설상화와 녹색의 통상화를 가진 홑꽃이다. 한 꽃당 설상화수는 18.8매이고 꽃의 크기는 7.1cm이었으며 초장은 130.0cm으로 줄기생장이 우수하였다. 그리고 흰녹병에 저항성이고 절화수명은 20.0일 정도였다. ‘Field Green’은 2013년 2월 20일 품종보호출원(출원번호: 2013-153)하고 국립종자원에 2014년 4월 2일 품종등록번호 제4839호로 품종보호등록을 완료하였다.

본 연구는 Veronica속 20종의 생장 및 개화 특성을 평가하기 위해 2년 동안 국립수목원 식물자원연구과 육종온실에서 수행하였다. Veronica속 식물은 다양한 생장 및 개화 특성을 가지고 있었다. 꼬리풀 20종을 식물 형태 및 초장에 따라 분류하였다. 포복형의 10cm 미만 초장은 V. armena와 V. repens, 직립형 30cm 미만 초장은 V. gentianoides ‘Little blues’ 등 4종, 직립형 30~60cm 사이 초장은 V. gentianoides ‘Blue Streak’ 등 7종, 직립형 60cm 이상 초장은 V. incana 등 7종이었다. 대부분의 엽색은 초록색이었고 V. incana와 V. incana ‘Silbersee’는 잎에 흰 털이 있었다. 꽃대 수는 5.3개부터 80.7개, 화수는 4.5개부터 67.3개였고 개화일은 3월 초순부터 6월 중순이었다. 화서의 형태는 총상화서, 수상화서, 취산화서로 분류하였다. 화색은 보라색 13종, 분홍색 2종, 흰색 5종이었다. 2017년 모든 개체가 개화한 종은 3종으로 V. longifolia ‘Blue Shades’, V. spicata ‘Blue Bouquet’, V. subsessilis ‘Blue Pyramid’였고, 일부 개체만 개화한 종은 7종으로 V. armena, V. gentianoides ‘Little Blues’, V. longifolia ‘Alba’, V. prostrata ‘Nestor’, V. spicata, V. spicata ‘Alba’, V. spicata ‘Sightseeing’ 이었다. 모든 개체가 개화하지 않은 종은 10종으로 V. gentianoides ‘Blue Streak’, V. incana, V. incana ‘Silbersee’, V. longifolia ‘Pink Shades’, V. orchidea ‘Blue Fingers’, V. repens, V. schmidtiana, V. spicata ‘Blue Carpet’, V. spicata ‘Pink Goblin’, V. teucrium ‘Royal Blue’였다. 반면, 2018년에는 모든 종의 개체가 개화하였다.

Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

Koreans considered themselves as a critical link between the past and future and not as an autonomous individual of now, in contrast to what many westerners emphasized and modern Koreans are beginning to think. This study is to apply the "Asian Jeong Nang silver model" as a spirit of "happiness and dignity of the elderly " as the basic policy for the aging Baby Boomers. The way of life of Jeju Haenyeos was similar to the typical life style of retired Baby Boomers, who had been devoted to social contribution until the last minute, returning expert knowledge to the community. That’s why Haenyeo community was chosen as a typical archetype, as well as other example, for a new silver model development. Cross Cultural Ageing Initiative (CCAI)’s purpose is to promote international exchange and collaboration between Korea and United States for research, education and service for the aging population. To bring together the best practices of the East and West, involving all the stake holders of the wider community such as participants in “the 2021 Global Ageing Network(GAN) Biennial Conference : East meets West: Approaches to Healthy Ageing and Wellness in both Seoul and Jeju Island in S. Korea September by GAN and GAN Korea. In particular, the upcoming “Silver Tsunami” in Korean society shows the necessity of welfare models for Korean baby boomers who have retired as highly educated professionals.

The CC chemokine receptor 5 (CCR5) is a G protein-coupled receptor that regulates chemotaxis and effector functions of immune cells. It also serves as the major co-receptor for the entry of human immunodeficiency virus (HIV). Recently, CCR5 inhibitors have been developed and used for the treatment or prevention of HIV infections. Additionally, it has been identified that CCR5 controls bone homeostasis by regulating osteoclastogenesis and the communication between osteoblasts and osteoclasts. However, the effects of CCR5 inhibition on bone tissue in elderly patients are unknown. This study aimed to examine the bone phenotype of aged CCR5 knockout (KO) mice. Femoral and tibial bones were isolated from 12-month and 18-month old wild-type (WT) and CCR5 KO mice, and microcomputed tomography and histology analyses were performed. Twelve-month-old CCR5 KO mice exhibited a decreased trabecular bone mass and cortical bone thickness in both femoral and tibial bones compared with agematched WT mice. Eighteen-month-old mice also showed a decreased trabecular bone mass in femurs compared with control WT mice, but not in tibial bones. Unlike in 12-month-old mice, the cortical margin of femurs and tibias in 18-month-old mice were rough, likely because they were aggravated by the deficiency of CCR5. Overall, our data suggest that the deficiency of CCR5 with aging can cause severe bone loss. When CCR5 inhibitors or CCR5 inactivating technologies are used in elderly patients, a preventive strategy for bone loss should be considered.

This study attempts to improve the understanding of the rapidly growing online video platform market such as Youtube and OTT, and to investigate the attributes and relative importance of them. For this purpose, the factors that influence the choice to use were derived through literature studies and the Focus Group Interview (FGI), and the priority of the factors was calculated through the analytic hierarchy process (AHP). The upper layer of the AHP structure was ‘Relationship’, ‘Entertainment’, ‘Informativity’, and ‘Convenience’, and the lower layer was structured into 13 elements. The importance priority analysis among the factors that influence the choice to use was done by teenagers, 20s, and 30s and the results are summarized as follows : First, Users consider the ‘Just for fun’ and ‘Satisfaction of interests’ as the most important factors, followed by ‘Easy accessibility to use’, ‘Vicarious satisfaction’, ‘Usefulness of Information’, and ‘Up-to-dateness of information’. Second, the ranking of the upper layer was in the order of ‘Entertainment’-‘Informativity’-‘Convenience’-‘Relationship’.As a result of AHP,‘Entertainment’ was 3.6 times more important than ‘Relationship’. In the comparison by age group, only teenagers regarded that ‘Convenience’ is more important than ‘Informativity’. According to the characteristics of the age group, the lower layer of teenagers consider ‘Convenient function’ to be important and ranked ‘Usefulness of information’ in 8th. While ‘Vicarious satisfaction’ ranked 4th out of 13 factors in the entire age group, those in their 20s and 30s ranked 8th, showing a difference. In the case of 20s, ‘Reasonable price’ was ranked 4th and the ‘Diversity of Information’ was ranked 5th, Otherwise 30s consider ‘Trustworthiness of Information’ to the third. Third, unlike ‘Convenience’ which was the lower-rank in the upper layer AHP analysis, ‘Easy accessibility to use’, the lower-layer of convenience, ranked third overall in the importance analysis among the 13 lower-layer factors, and showed a similar patterns in the age groups results. In the conclusion, this study demonstrates that ‘Convenience’ and ‘Vicarious satisfaction’ factors, which were not relatively well addressed in the previous studies, are the key factors to be considered in. By presenting the results of the importance analysis on each of the selected attributes, This study has a practical implication that Industries such as on-line video service platform provider can use the importance priority in establishing the directions of future strategy.

Objective: To evaluate item-factor structures of the 15 sensory items from the Survey of Pathways to Diagnosis and Services (Pathways) and examine the best fitting model. Methods: The study subjects were 1,968 children aged 6-17 years, who had ever been diagnosed with Autism Spectrum Disorder (ASD) and/or Intellectual Disability (ID). Factor analyses and item response theory models were used to determine the best fitting item-factor structure of the sensory items. The Strengths and Difficulties Questionnaires (SDQ) was used to test the concurrent validity of the sensory severity estimates. Results: A bifactor MIRT model (a general and four sensory factors) was selected as the best fitting model. All items statistically fitted to the bifactor model (p > .01) and showed moderate correlations with all five subscales of the SDQ (r s = .31 ~.51, p < .0001). The general sensory score differentiated the four different diagnostic groups (ASD, ID, ASD with ID, and no current symptoms) [F (3,1961) = 207.4, p < .0001]. Conclusion: The Pathways sensory items yielded reliable estimates of the general and each corresponding sensory factor by applying a bifactor MIRT model. The sensory score can be used as a valid sensory measure in the population survey.

Berberine has been used clinically for more than a decade to treat various diseases, has been shown to be effective in osteoblast differentiation, and is a potential treatment option for osteoporosis. However, compared with existing osteoporosis drugs, berberine is somewhat less effective. This study aimed to identify a new compound with efficacy superior to that of berberine. The osteogenic activities of various berberine derivatives were evaluated via cell differentiation in C2C12 preosteoblast cell lines. Alkaline phosphatase (ALP) staining assay and structure– activity relationship demonstrated that compound 2b had a potent osteogenic effect. Furthermore, compound 2b dose dependently increased ALP activity and showed no toxicity at the effective concentration, indicating its efficacy. Additionally, compound 2b upregulated BMP2-induced transcriptional activity in a promoter activity assay using ALP, BSP, and OC promoters.

Osteoarthritis occurs when the cartilage that gradually deteriorates as common aging-associated disease in humans and animals. There is no cure, but the treatments are available to manage to relieve pain through medication such as steroids. Growing interest has been focused on the role of cell-based therapies using mesenchymal stem cells (MSCs). In addition, mesenchymal stem cells can be isolated from almost adult tissues and known for their potential of becoming cartilage. Clinical and experimental studies indicate that the development of treatment using stem cells is double-edged sword involving a possibility such as tumorigenesis. This study focused on the electrical features during articular cartilage development and hypothesized that external electric fields promote pre-chondrogenic condensation without concern relating to genetic modification or exogenous factors. Here, it has been reported that exogenous direct electric fields drive pre-chondrogenic condensation which is the stage where cartilage formation begins by condensation of stem cells and cartilage cells in the microenvironment of the joint. Time-dependent observations also support the contribution of electrical stimulation (ES) to induce gradual aggregation of MSCs into highly compact structures within 3 days. Collectively, our findings provide the potential of electrical stimulation-driven chondrogenesis of mesenchymal stem cells in the absence of exogenous factors for repair of cartilage defects.

Canine parvovirus type-2 (CPV-2) has been reported worldwide as the main agent associated with acute hemorrhagic enteritis, resulting in high morbidity, especially in young dogs. CPV-2 has three genetic variants, 2a, 2b, and 2c. Here, we report three cases of canine parvovirus enteritis associated with CPV-2a (2 samples) and -2c (1 sample) infections that occurred in three young dogs suffering from enteritis. Isolates from dog diarrheic fecal samples were sequenced by polymerase chain reaction (PCR) and identified as two types of CPV-2a and one type of CPV-2c. This work constitutes the first isolation and genetic characterization of CPV-2c in the Republic of Korea.

The advance of information technology with governmental support allows researchers to utilize big data to conduct rigorous rehabilitation-related studies in both the United States and Korea. While big data research has been actively conducted in the United States, few Korean rehabilitation scientists currently conduct big data research. In this paper, we discuss the existing rehabilitation-related big data in Korea and introduce the current big data research trends in the United States. In addition, we provide potential research areas by discussing the previous big data studies in the United States, including 1) functional outcomes, 2) geographical variation, 3) linkage of a population-based cohort to hospital data, 4) cross-national comparison, and 5) scale development. We also discuss various statistical methods required in big data research and compare how these methods differ compared to the traditional research methods in rehabilitation research. Lastly, we discuss the importance of workforce development in rehabilitation-related big data research. We hope this paper will provide occupational therapy scientists and clinicians with practical and useful information about big data research and accelerate big data rehabilitation research communities in Korea.

Alopecia has emerged as one of the biggest interests in modern society. Many studies have focused on the treatment of alopecia, such as transplantation of hair follicles or inhibition of the androgen pathway. Hair growth is achieved through proper proliferation of the components such as keratinocytes and dermal papilla cells (DPCs), movement, and interaction between the two cells. The present study examined the effect of the hedgehog (Hh) signaling pathway, which is an important and fundamental signal in the cell, on the morphology and the viability of human keratinocytes and DPCs. Upregulation of Hh signaling caused a morphological change and an increase in epithelium-mesenchymal transition-related gene expression but reduced the viability of keratinocytes, while the alteration of Hh signaling did not cause any change in DPCs. The results show the possibility that the regulation of Hh signaling can be applied for the treatment of alopecia.

본 연구는 학교 장면에서 학생지도 시 교사의 상업용 온라인 게임 활용 의사에 영향을 미치는 요인이 무엇인지, 그리고 각 요인들이 어떠한 영향을 미치는지를 알아보기 위해 수행되었다. 이를 위해 면대면 설문조사를 실시하였고, 전국 초, 중, 고등학교 교사와 상담교사, 전문 상담사, 총 236명의 자료가 분석되었다. 교사의 인구통계학적 특성과 교사의 상업용 온라인 게임 활용 의사의 관련성을 분석해본 결과, 남성보다 여성교사가, 교사의 연령이 높고 근무 기간이 길수록 상업용 온라인 게임 활용 의사가 낮은 것으로 확인되었다. 교사의 상업용 온라인 게임 활용 의사에 영향을 미치는 요인을 중다회귀분석방법을 통해 분석한 결과, 교사의 개방성과 디지털 게임에 대한 태도 중 디지털 게임의 유익성에 대한 인식, 디지털 미디어 활용능력이 학생지도 시 교사의 상업용 온라인 게임 활용 의사에 정의 영향을 미치는 것으로 확인되었다. 본 연구는 교과학습 및 학생 생활 지도에 있어 교육적 잠재력을 가지고 있는 상업용 온라인 게임에 대한 교사의 활용 의사를 촉진하는 주요 요인들을 밝혔다는데 그 의의가 있다.

A warehouse is more than just a place to store goods but also creates a time utility gap between production and consumption that is important in the development of trade and commerce. Malaysia has a high aspiration to be the most popular gateway in Asia. However, Malaysia Productivity Corporation has claimed that particularly in efficiency and productivity aspects, the warehouse industry is less incline to innovate and make improvement to increase. There are ten failure factors that had been identified during literature survey affecting to the warehouse operations efficiency. Therefore, this study intends to analyse and select the main failure factors that mostly affecting the warehouse operations efficiency. A Fuzzy Analytic Hierarchy Process (FAHP) technique has been used in this study to calculate the weight value of each variable through a pair-wise comparison method. The finding highlighted the four main failure factors which are ‘equipment’, ‘environment concern’, ‘special handling consideration’ and ‘human factor’ that influence directly to the warehouse operations efficiency. This paper has contributed to a new knowledge of complex warehouse operation area with recommendations action that shall be taken to manage the possible risk in future, which will also contribute to achieve the government goals.

At present, ECDIS has been widely used in ship positioning, collision avoidance and navigation, and has developed into an important part of integrated navigation and automation system. However, most ECDIS software focuses too much on system function and technical level when realizing powerful navigation AIDS, neglecting the problems that the crew are most concerned about, such as realizing the human-computer interface operation quickly and simply as well as obtaining the information of human-computer interaction effectively and accurately. In order to improve the design of Electronic Chart Display and Information system（ECDIS）human-computer interface and its usability effectively, a new method for evaluating ECDIS human-computer interface is proposed. Starting from the concept and connotation of usability, five-factor personality model is used to measure the users’ personality type in reference to the essence of usability (user’s emotional experience) and calculate the basic emotional variant of users. Then, according to the users’ emotional stimulation after finishing the given ECDIS task and combining with the system Usability scale (SUS), a tool dedicated to usability testing and five dimensions of usability evaluation proposed by Jakob Nielsen, father of usability, the emotion relationship model of usability and users is established by using partial least square method ( PLS ), and the validity of the model is verified with an aim to locate the key indicators that affect the usability of the ECDIS human-computer interface, It will be more helpful to improve the usability of ECDIS human-computer interface.

The act of refusal takes place in everyday lives, but it has been discussed to be a difficult speech act for nonnative speakers. This study seeks to investigate Korean EFL learners’ refusals in extended discourse along with their pragmatic perceptions. Sixteen college students were engaged in oral role-plays with two native English speakers and instructed to refuse the interlocutor’s request. The refusal performances were analyzed using conversation analysis framework with respect to the interlocutors’ different powers and the learners’ proficiency levels. Learners also participated in retrospective verbal reports. Findings depict different verbal and nonverbal features illustrating learners’ sensitivity towards higher status and their different linguistic abilities. Furthermore, their verbal reports revealed that despite their pragmatic awareness, learners were not fully equipped with appropriate L2 pragmatic knowledge.

Selectins are cell membrane glycoproteins that recognize specific glycoconjugates expressed on the surface of cells. Then, selectins adjust cell-cell interactions that are important in inflammation, hemostasis and cancer metastasis. Selectins mediate leukocyte calls to move into the site of inflammation through interactions with activated endothelial cells or endogenous selectin ligands expressed in high endothelial venules. Types of selectins are divided into L-selectin, E-selectin and P-selectin, which are called to CD62L, CD62E, and CD62P, respectively. Each selectin is composed of four regions; the C-type lectin region of N-terminal, the epidermal growth factor (EGF) region, the intracellular C-terminal region, and the hydrophobic transmembrane region. They have similar structures but differ in their binding specificities and tissue distributions. The selectin family commonly recognizes the sialyl Lewis X (sLeX) on carbohydrate structures. Although biological ligands bound to each selectin are different from each other, they commonly bind to P-selectin glycoprotein ligand-1 (PSGL-1) ligand. The PSGL-1 ligand is a glycoprotein promoting cell adhesion in inflammatory responses. If the absence of selectins and their ligands in humans and animals are, should lead to persistent infections and diseases. Selectin family must be considered as a key subject for drug discovery since they have various functions depending on the ligand which they bind to.

Maritime transportation plays a significant role in global economies. Seaports as primal nodes in maritime supply chain are also indispensable in ensuring efficient and effective global trading. On the other hand, seaport inefficiencies have profound influences on the overall performance which can cause congestion, operational disruption, high cost expenses, unnecessary wastes and environmental pollution, which eventually resulting significant financial losses. As a result, business and environmental sustainability will not be achieved. In order to overcome these hindrances, a novel performance model of Lean, Agile, Resilience and Green (LARG) is proposed as management tools for enhancing the business and environmental sustainability in seaport supply chain operations. The compatibility of LARG paradigms in seaport supply chain need to be identified and analysed to achieve the research aim. This paper employed a compatibility analysis for the new LARG paradigm for enhancing seaport supply chain practices. In this analysis, all potential paradigms are thoroughly reviewed and further validated by the domain experts consisting of academic and industry experts. The result of this paper shows that 17 selected LARG paradigms are compatible with 23 seaport supply chain practices respectively. For future research, the identified paradigms can be further investigated for many purposes such as measuring their influence on seaport supply chain practices and even assessing their applicability. It is worth mentioning that this research outcomes can assist Malaysian seaport practitioners to develop an enhanced management paradigm to boost their performance based on LARG model. Moreover, this model also can be applied globally as it is able to be adapted, revised and adjusted to suit the seaport preferences. As a result, this model able to enhance business capabilities, operational efficiencies and competitive advantages of seaport supply chain operations globally.