In this paper to prevent human errors analyzed the causes of railway accidents and human error in last 5 years(2007~2011). The 2nd Railway Comprehensive Safety Plan currently being implemented in the safety business for prevention of human error. The accidents are often resulted from multiple causes with hardware failure and human errors. And prevention of human error associated with the implementation details of the priority projects, 14 projects were selected by draw. Then Analytic Hierarchy Process(AHP) methodology was used to select what projects were effective to human error.

The nasal cavity encounters various irritants during inha¬lation such as dust and pathogens. To detect and remove these irritants, it has been postulated that the nasal mucosa epithelium has a specialized sensing system. The oral cavity, on the other hand, is known to have bitter taste receptors (T2Rs) that can detect harmful substances to prevent ingestion. Recently, solitary chemosensory cells expressing T2R subtypes have been found in the respiratory epithelium of rodents. In addition, T2Rs have been identified in the human airway epithelia. However, it is not clear which T2Rs are expressed in the human nasal mucosa epithelium and whether they mediate the removal of foreign materials through increased cilia movement. In our current study, we show that human T2R receptors indeed function also in the nasal mucosa epithelium. Our RT-PCR data indicate that the T2R subtypes (T2R3, T2R4, T2R5, T2R10, T2R13, T2R14, T2R39, T2R43, T2R44, T2R 45, T2R46, T2R47, T2R48, T2R49, and T2R50) are expressed in human nasal mucosa. Furthermore, we have found that T2R receptor activators such as bitter chemicals augments the ciliary beating frequency. Our results thus demonstrate that T2Rs are likely to function in the cleanup of inhaled dust and pathogens by increasing ciliary movement. This would suggest that T2Rs are feasible molecular targets for the development of novel treatment strategies for nasal infection and inflammation.

The use of high throughput screening (HTS) in drug development is principally for the selection new drug candidates or screening of chemical toxicants. This system minimizes the experimental environment and allows for the screening of candidates at the same time. Umbilical cord-derived stem cells have some of the characteristics of fetal stem cell and have several advantages such as the ease with which they can be obtained and lack of ethical issues. To establish a HTS system, optimized conditions that mimic typical cell culture conditions in a minimal space such as 96 well plates are needed for stem cell growth. We have thus established a novel HTS system using human umbilical cord derived-mesenchymal stem cells (hUC-MSCs). To determine the optimal cell number, hUC-MSCs were serially diluted and seeded at 750, 500, 200 and 100 cells per well on 96 well plates. The maintenance efficiencies of these dilutions were compared for 3, 7, 9, and 14 days. The fetal bovine serum (FBS) concentration (20, 10, 5 and 1%) and the cell numbers (750, 500 and 200 cells/well) were compared for 3, 5 and 7 days. In addition, we evaluated the optimal conditions for cell cycle block. These four independent optimization experiments were conducted using an MTT assay. In the results, the optimal conditions for a HTS system using hUC-MSCs were determined to be 300 cell/well cultured for 8 days with 1 or 5% FBS. In addition, we demonstrated that the optimal conditions for a cell cycle block in this culture system are 48 hours in the absence of FBS. In addition, we candidates using our HTS system which demonstrates the feasibility if using hUC-MSCs for this type of screen. Moreover, the four candidate compounds can be tested for stem cell research application.

선행 연구에서 형질전환 모상근 대량생산 최적배지 선발을 통해 WPM(Woody Plant basal salt Mixture) 배지가 형질전환 모상근 대량생산의 최적배지임을 확인할 수 있었다(Kim et al., 2012). 이에 당, 탄소원, pH, 무기염, 식물 호르몬 등이 형질전환 모상근의 수량성에 미치는 연구를 통해 최적의 형질 전환 모상근 대량생산 조건을 확립한 결과는 다음과 같다. 1. 형질전환 모상근 대량생산에 적합한 pH는 7.0이었으며, 최적의 탄소원과 농도는 1%의 sucrose임을 확인하였다. 2. 형질전환 모상근 대량생산에 적합한 적정 무기염들 (KH2PO4,NH4NO3, KNO3)의 농도는 KH2PO4 경우 MS배지와같은 농도인 0.94 mM에서 수량성이 303 g으로 가장 높았고, NH4NO3 및 KNO3 농도는 MS배지 농도의 1/4배인 3.87 mM, 3.52 mM 처리가 345 g과 358 g으로 수량성이 가장 높았다. 3. 형질전환 모상근 대량생산에 있어 식물생장조절물질 NAA, IBA의 최적 농도는 무처리 경우가 각각 595 g과 402 g으로 수량성이 가장 높아 대량생산에는 식물생장조절물질 처리가 필요 없을 것으로 판단되었다.

Human protein C (hPC) is a regulator of homeostasis, suggesting its potential use as a therapy for many disease states. Protein C is a zymogen of a serine protease that is activated by thrombin. Protein C, also known as autoprothrombin ⅡA and blood coagulation factor ⅪⅤ, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. hPC is a 62 KD disulfide- linked heterodimer consisting of a 21 KD light chain and a 41 KD heavy chain which circulates as an inactive zymogen in plasma. In this study, we focus on generation of hPC transgenic mice. hPC transgenic mice were produced by using micro-injection method. The hPC cDNA was cloned into pBC1 vector under goat β-casein promoter. One-cell stage embryos microinjected were transferred to 24 recipient mice on day 1 of the estrus cycle. We screened 61 mice by the PCR. Four line transgenic mice were identified and confirmed expression of protein C gene in mammary gland and several organ. We also analyzed the expression of mRNA and protein through the northern blot and western blot in mammary gland of hPC transgenic mice. hPC was localized in the alveolar epithelial cell by immunohistochemistry. Now, we are collecting the milk from the 2 found lines. After then, we are checking the activity of hPC produced from mice milk.

The objective of this study was to investigate the proteome composition in pretermand term‐derived human umbilical cord. Umbilical cord samples were collected from 6 preterm infants with gestational age less than 36 weeks or 4 full terms together with medical information during prenatal period. Several biomarkers are routinely used clinically for predicting preterm labor; however, these factors are either nonspecific or detected too late. Protein profiles were performed on samples from both preterm‐ and termderived human umbilical cord by using Two‐dimensional gel electrophoresis (2‐DE). Approximately 200 different proteins were identified between preterm‐ and term‐delivered umbilical cords. Among them, differentially expressed 34 proteins were identified in 48 protein spots. In the preterm‐derived human umbilical cords, 15 proteins were present at higher levels (2.0‐ to 9.28‐fold increases) and 19 were present at lower levels (2.0‐ to 11.8‐ fold decreases or not detectable) compared to the control term‐derived umbilical cords. Proteomics approaches such as 2‐DE could greatly facilitate the discovery of new and better biomarkers. The high sensitivity and specificity achieved by the combined use of the selected biomarkers show great potential for the early detection of Adverse pregnancy outcomes such as pre‐eclampsia, small for gestational age infants, preterm delivery and placental abruption are associated with higher mortality. Increased amount of HIF‐1 α, GAPDH and HSP27 were observed in preterm‐derived umbilical cords was due to hypoxia‐ dependant and oxidative stress‐independent manner. Moreover, we isolated HUVEC (Human umbilical vascular endothelial cells) from preterm‐ and term‐derived umbilical cords and examined LDH activity. The results of the current study may provide important insights into the molecular mechanisms underlying umbilical cord development and also these data will contribute to a better understanding of the composition of preterm‐ and term ‐ derived human umbilical cord and aid the discovery of novel biomarkers for the prenatal diagnosis of fetal abnormalities

Cordycepin, a specific polyadenylation inhibitor, is the main functional component in Cordyceps militaris that is one of the top three renowned traditional Chinese medicines. In this study, we performed in vitro experiments to investigate the anti-invasive and anti-metastatic activities of cordycepin using human prostate carcinoma LNCaP cells. Cordycepin were administered and their effects on LPS-induced cell migration and invasion by wound healing migration assay, measurement of TER and In vitro invasiveness assay. Within the concentrations which were not cytotoxic effects, cordycepin caused a concentration-dependent suppression of LPS-induced cell migration and invasion. The anti-invasive activity of cordycepin was also found to be associated with increased tightness of the TJ, which was confirmed by an increase in TER. The activity of MMP-2 in LNCaP cells was dose-dependently inhibited by treatment with cordycepin, and this was also correlated with a decrease in expression of its mRNA and proteins, and up-regulation of TIMPs expression. Additionally, cordycepin repressed the LPS-induced NF-kB activation and phosphorylation of PI3K/AKT. Taken together, these findings suggest that cordycepin inhibited LPS-induced migration and invasion of LNCaP cells by down-regulating the expression and activity of MMP-2, and the possible targets may be NF-kB and PI3K/AKT.

긴 역사를 통해 발전되어 온 인권과 자연법은 세계 모든 사람이 누려야 하는, 빼앗지 못할 필수 권리에 대한 내용을 담고 있다. 유럽에서는 크리스천 종교가 꽃을 피우기 전에 이미 고대부터 소포클레스와 플라톤을 비롯한 그리스와 로마 철학자들은 인간이 원래 자연적으로 누려야 할 기본권이 있다고 주장한 바 있다. 시간이 흐르면서 신학의 아버지로 불리우는 아퀴나스는 이를 더욱 발전시켜 인간은 신으로부터 태어나면서부터 받게 되는 신성한 권리를 누려야 하며 정부는 이런 권리를 법으로 인정해야 한다고 하였다. 그 이후 프랑스와 미국의 독립운동가, 또한 칸트와 같은 독일 철학자들은 이러한 이론을 민주정치로 현실화시키는데 앞장섰다. 현재는 거의 모든 민주국가의 헌법과 또한 국제법이 자연적 인권을 법의 기반으로 인정하고 있다. 오늘의 우리 사회는 인권과 자연법의 중요성을 이해하고 앞으로 이 전통을 이어나가 인간을 위한 법과 정책을 만들어야 한다.

해양운송분야에서 인적 오류의 비중 및 피해가 막대하지만, 선박운항자 관점에서의 안전성 평가를 위한 구체적인 수단이나 기술의 개발은 미흡하며, 해양사고의 예방을 위한 인간공학적 시험평가 기법 및 항해장비와의 인터페이스 설계를 위한 관련 연구가 미흡하다. 즉, 해상교통 안전성 평가를 위한 선박운항 시뮬레이터의 활용도와 신뢰성을 높이기 위한 정량적 지표의 개발이 시급히 요구되고 있다. 본 논문에서는 해상교통 안전성 평가를 위한 인간공학적 실험평가 기

Dlx3 and Dlx5 are homeobox domain proteins and are well-known regulators of osteoblastic differentiation. Since possible reciprocal relationships between osteogenic and adipogenic differentiation in mesenchymal stem cells exist, we examined the regulatory role of Dlx3 and Dlx5 on adipogenic differentiation using human dental pulp stem cells. Over-expression of Dlx3 and Dlx5 stimulated osteogenic differentiation but inhibited adipogenic differentiation of human dental pulp stem cells. Dlx3 and Dlx5 suppressed the expression of adipogenic marker genes such as C/EBPα, PPARγ, aP2 and lipoprotein lipase. Adipogenic stimuli suppressed the mRNA levels of Dlx3 and Dlx5, whereas osteogenic stimuli enhanced the expression of Dlx3 and Dlx5 in 3T3-L1 preadipocytes. These results suggest that Dlx3 and Dlx5 exert a stimulatory effect on osteogenic differentiation of stem cells through the inhibition of adipogenic differ¬entiation as well as direct stimulation.

본 연구는 식품 관련 기업과 공공기관에서 요구되는 인력의 역량 항목과 전공과목의 업무 관련 중요도를 조사하고, 여러 종류의 식품 관련 자격증에 대한 가치를 추정하여 식품 관련 인력의 수요를 예측하고자 하였다. 또한 설문 조사 결과의 신뢰성을 높이기 위해 식품 기업과 공공 기관의 업무 경험이 적어도 3년 이상된 실무자들을 대상으로 설문 조사를 실시하였다. 기업 및 공공기간에서 신입사원 채용 판단 기준 분석에 있어서 가장 중요하게 보는 것은 인성 및 성격, 식품전문지식 및 컴퓨터 능력의 순으로 나타났다. 기업별 및 공공기관으로 나누어 볼 때, 공공기관에서는 식품전문지식, 출신학교 및 학력이 기업군에 비해 중요하게 여기고 있으며, 자격증은 위탁급식업체에서 가장 중요하게 판단하는 것으로 나타났다. 업무와 전문과목 관련 중요도 분석 결과, 법규 및 위생 관련 과목이 중요하게 판단되는 것으로 나타났다.

MicroRNAs (miRNAs) are small non-coding RNAs that mediate gene expression at the post-transcriptional level by degrading or repressing targeted mRNAs. These molecules are about 21-25 nucleotides in length and exert their effects by binding to partially complementary sites in mRNAs, predominantly in the 3'-untranslated region (3'-UTR). Recent evidence has demonstrated that miRNAs can function as oncogenes or tumor suppressors through the modulation of multiple oncogenic cellular processes in cancer development, including initiation, cell proliferation, apoptosis, invasion and metastasis. In our present study, we examined the expression profile of miRNAs related to oral cancer cell growth inhibition using normal human oral keratinocytes (NHOK) and YD-38 human oral cancer cells. By miRNA microassay analysis, 40 and 31 miRNAs among the 1,769 examined were found to be up- and down-regulated in YD-38 cells compared with NHOK cells, respectively. Using qRT-PCR analysis, the expression levels of miR-30a and miR-1246 were found to be increased in YD-38 cells compared with NHOK cells, whereas miR-203 and miR-125a were observed to be decreased. Importantly, the overexpression of miR-203 and miR-125a significantly inhibited the growth of YD-38 cells. This finding and the microarray data indicate the involvement of specific miRNAs in the development and progression of oral cancer.

감성과학에서 인간의 감성을 손쉽게 측정할 수 있는 기술이나 기법 등은 매우 중요한 분야이다. 최근 마음의 변화는 몸의 변화를 수반한다(정신신경증면역내분비학, psychoneuroimmunoendocrinology)는 전제하에 인간 감성을 체액을 통해서 측정하는 시도가 이루어지고 있다. 몸의 변화 중에 최근까지 가장 많이 연구된 것은 스트레스에 관한 것으로 이를 측정하는 지표로는 코티졸(cortisol)이라는 물질이 널리 알려져 오고 있다. 코티졸을 측정하는 기존의 방법을 열거하면 High Performance Liquid Chromatography (HPLC), fluorometric assay, reverse phase chromatography 등이 대표적인데 모두 시간이 많이 걸리고 가격이 비싸며 휴대용이 아니기에 POCT (point of care testing)에 적합하지 않다. 이에 본 연구진은 소형 초고주파 공진 소자를 만들어 타액 속의 코티졸을 측정할 수 있는 항체를 고정하고 이것이 코티졸과 결합할 때 나오는 공진신호를 읽음으로써 환자의 타액 속 코티졸을 쉽고 빠르게 측정할 수 있는 기법을 소개한다. 본 연구를 위해 제안된 공진소자는 밀리미터 크기의 소자로서 제작이 용이하며 간단한 형태이다. 최종적으로 소자표면상에 결합되는 코티졸 농도변화(100, 10, 1, 0.1 ng/ml)에 따라 거의 선형적인 주파수응답(11, 10, 9, 7 MHz) 특성을 보인다. 100 pg/ml에 해당하는 적은 코티졸의 양까지 거의 실시간에 가까운 빠른 시간 안에서 쉽게 검출이 가능하며, 뿐만 아니라 표지(labeling)가 필요 없는 장점을 가지고 있다. 이러한 농도에 따른 주파수 변화를 기반으로 하는 코티졸 감성지표센서는 무선단말시스템에 적용될 수 있는 가능성과 응용성을 가지고 있다.

Cruciferous vegetables including diindolylmethane (DIM) have been shown to have anticancer activity. Especially, DIM-pPhBr and DIM-pPhF used in this study was reported to have more effective and less toxic effects than DIM. However, there is no report presenting their anti-tumorigenic activity in oral cancer. In the present study, we examined the effects of DIM-pPhBr and DIM-pPhF on the cell proliferation and apoptosis in KB human oral cancer cells. DIM-pPhBr and DIM-pPhF decreased cell proliferation and induced apoptosis evidenced by western blot analysis, DAPI staining and sub-G1 population. This provides the first evidence that DIM-pPhBr and DIM-pPhF originating from cruciferous vegetables induce apoptotic cell death in human oral cancer cells to inhibit cancer cell proliferation.

Polybrominated diphenyl ethers (PBDEs) are a class of "brominated" (bromine containing) man-made chemicals used as flame retardant additives in plastics, foams, and textiles. PBDEs are found in various environmental contaminants in air, soil, sediment, and water, and 209 individual forms (congeners) of PBDE exist. Among these, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) is the dominant congener found in the environment. Exposure to BDE-47 is now worldwide, and levels of BDE-47 have been detected in the blood of animals, including humans. BDE-47 can adversely affect the developmental system in both humans and animals. BDEs have structural similarities to polychlorinated biphenyls and thyroid hormones. However, recent studies have shown that BDEs may act as hormonal disrupting chemicals with detrimental effects. Therefore, a reliable assessment of BDE-47 toxicological action is required to understand the detrimental impacts of BDE-47 on human health. In this review, we overview recent studies on the distribution and potential toxicological effects of BDE-47 in humans and animals.