Background: The consequences of falls are often debilitating, and prevention is important. In theory, the lower the center of mass (COM), the greater postural stability during standing, and a weight belt at the waist level may help to lower the COM and improve the standing balance. Objects: We examined how the limit of stability (LOS) was affected by the lowered center of mass with the weight belt. Methods: Twenty healthy individuals participated in the LOS test. After calculating each participant’s COM, a weight belt was fastened ten centimeters below the COM. Trials were acquired with five weight belt conditions: 0%, 2%, 4%, 6%, and 8% of body weight. Outcome measures included reaction time, movement velocity, endpoint excursion, maximum excursion, and directional control in 4 cardinal moving directions. Results: None of our outcome variables were associated with a weight belt (p > 0.075), but all of them were associated with moving direction (p < 0.01). On average, movement velocity of the COM and maximum excursion were 31% and 18% greater, respectively, in mediolateral than anteroposterior direction (5.4°/s vs. 4.1°/s; 97.5% vs. 82.6%). Conclusion: Our results suggest that postural stability was not affected by the weightinduced lowered COM, informing the development and improvement of balance training strategies.

Plant regeneration from stems and leaves was carried out for the growth of biomass, hybrid poplar(Populus alba x P. glandulosa)clone, which has various uses for plant purification. Callus was well induced when stem and leaf tissues were cultured in 1.0 mg/L 2,4-D containing MS medium. Shoot regeneration was best induced by zeatin among growth regulators, and Plant regeneration was more regenerated in leaf-derived callus than in stem-derived callus. The growth of regenerated shoots at high and low concentrations of zeatin was similar to that of the control at low concentrations. As the concentration of growth regulators increased, the growth of regenerated shoots showed a big difference among individuals. Hybrid poplar showed color variation of plant stem in medium containing high concentration of growth regulator. Regenerated individuals were in vitro rooted in MS medium containing 0.5 mg/L IBA after 2 weeks of culture. and transferred to the greenhouse for acclimatization. The study is believed to be widely used for the induction of in vitro variants through organogenesis.

The plastic waste problem is deepening all over the world. Plastic wastes have serious impacts on our lives as well as environmental pollution. The production and use of plastics increases every year, but once they are produced, they usually roam the earth for hundreds or thousands of years to pollute the environment. Although there is growing interest in plastic issues around the world and environmental regulations are being tightened, but no clear solution has yet been found. This study suggests Environmental degradation index (EDI). EDI can help raise consumers’ attention to plastic wastes. In addition, EDI will contribute to reduce them in the future. As far as we know, this is the first study. We developed EDI for the confectionery packaging. This study defines four factors that may affect the environment of confectionery packaging: greenhouse gas emissions, energy consumption, methane emissions, and packaging space ratio. Then we quantify the value of each element and compute EDI as the sum of the four component values. In order to evaluate the feasibility of EDI proposed in this study, confectionery-packaging materials distributed in Korea were collected and analyzed. First, the types of confectionery are classified into pies, biscuits, and snacks and basic data was collected. Then the values of the four components were calculated using existing research data on the environment. We can use the proposed EDI to determine how much a product packing affects the environment.

In previous studies, we found the production of antibodies against cross-reactive bovine serum albumin (BSA) in D-galactose (D-gal) induced aging mouse models. We performed immunoblot analysis with mouse tissue lysates to investigate the changes in the overall autoantibody production in this animal model. And we were able to see the possibility of altering the activity of mouse natural antibodies in this process. In this study, we examined changes in existing natural antibodies in a D-gal-induced aging mouse model. Serum samples were collected from 3-week-old mice (3w), 13-week-old mice (13w), and 13-week-old mice that were treated with D-gal for 6 weeks (13wDG), beginning at the age of 8 weeks. Levels of immunoglobulins (IgM, IgG, and IgA) were quantitatively analyzed in serum samples. Tissue samples were obtained from skin, spleen, and ovary for Western blotting analyses. Natural antibody activity was examined by enzyme-linked immunosorbent assay analyses of anti- double-stranded DNA (dsDNA) antibody. Western blotting analyses using mouse tissue lysates showed that several protein bands detected by serum antibodies from 3w mice became increasingly thicker when detection was performed with serum samples from 13w and 13wDG mice, indicating quantitative increases in levels of natural antibodies. Relative amounts of total IgG, IgM, and IgA immunoglobulins sequentially increased in serum samples from 3w, 13w, and 13wDG mice. A similar tendency was observed regarding the levels of IgM and IgG antibodies against dsDNA. These results indicate increased levels of natural antibodies in the D-gal-induced aging mouse model. Therefore, this animal model could be useful for future natural antibody research.

Atopic dermatitis (AD) usually develops in patients with an individual or family history of allergic diseases, and is characterized by chronic relapsing inflammation seen specially in childhood, association with IgE hyperproduction and precipitation by environmental factors. and wished to examine closely effect that Polygonum multiflorum isolated PM-E and PM-70M orally adminstration used to atopy dermatitis disease patient get in atopy eruption control experimentally. Atopic dermatitis is a chronically relapsing inflammatory skin disease. Animal models induced by relevant allergens play a very important role in the elucidation of the disease. This study was investigated the anti-allergic effect of PM-E and PM-70M on BMAC induced atopic dermatitis in NC/Nga mice. We summerized as the follow. PM-E and PM-70M significantly reduced the skin number of total cell number, CD4+ and CD11b+/Gr-1 cell compared with positive control and decreased the invasion of CD4+ cell in dorsal skin tissue compared with positive control group by using immunohistochemical staining and chemokine such as eotaxin and CCR3 compared with positive control group. PM-E and PM-70M markedly suppressed invasion and edema of leukocytes and mast cell in dorsal skin. Taken together, these findings suggested that PM-E and PM-70M has an anti-allergic activity and this might be useful for the clinical application to treat allergic diseases such as atopic dermatitis.

This study examined the changes in the protein and mineral composition of Gryllus bimaculatus fermented with Bacillus substilis and the mycelia of Basidiomycetes. Normal Gryllus bimaculatus (S) and experimental group data obtained after an inoculation of Bacillus substilis (SC) (KACC 19623), Pleurotus eryngii (SP) and Cordyceps millitaris (SC) were compared. The crude protein content of the Gryllus bimaculatus (control) was 75.48%, but it decreased to 64.55, 54.32, and 63.53% after fermentation with SB, SP and SC, respectively (p<0.05). An analysis of the organic elements showed that the contents of the carbon and nitrogen sources were also reduced after fermentation, and the most significant decrease was observed after fermentation with SP. In SDS-PAGE, a 120 kDa and a 48 kDa protein of Gryllus bimaculatus were found. On the other hand, protein bands faded after fermentation with SP and SC, respectively. Moreover, no visible band was observed after fermentation with SB. According to amino acid analysis, the total free amino acid content increased 3.84 and 1.74 times after fermentation with SB and SP, respectively, compared to the corresponding baseline data. In contrast, it decreased by 0.52 times after fermentation with SC. Among the essential amino acids found in crickets fermented with SB, the valine and isoleucine content was 3.57 and 2.64 times higher, respectively, than the recommended daily amount of essential amino acids.

Brucellosis is the most common zoonosis worldwide, which is caused by Brucella spp. In humans, it can be mainly occurred by direct contact with infected animals or consumption of contaminated dairy products. This study focused on human brucellosis caused by B. melitensis discovered from Chinese worker in Korea in 2015. We investigated molecular epidemiological evidence to find the infection source. We first performed several PCR methods including 16S rRNA PCR, multiplex PCR and real-time PCR to identify Brucella species. We also conducted MLVA typing for epidemiological trace-back analysis. The isolate from the patient was confirmed to B. melitensis through Brucella-specific PCR. In clustering analysis with B. melitensis from foreign countries, this human isolate was correlated with B. melitensis isolates from humans and sheep in China by 99.9% similarity. Thus, we assumed the brucellosis patient has been already infected in China followed by migration to Korea according to molecular epidemiological analysis with history evidence. Moreover, we suggest it needs to take measures to reduce the risk for intercountry transmission of brucellosis due to the influx of infected people from abroad.

Process mining is an analytical technique aimed at obtaining useful information about a process by extracting a process model from events log. However, most existing process models are deterministic because they do not include stochastic elements such as the occurrence probabilities or execution times of activities. Therefore, available information is limited, resulting in the limitations on analyzing and understanding the process. Furthermore, it is also important to develop an efficient methodology to discover the process model. Although genetic process mining algorithm is one of the methods that can handle data with noises, it has a limitation of large computation time when it is applied to data with large capacity. To resolve these issues, in this paper, we define a stochastic process tree and propose a tabu search-genetic process mining (TS-GPM) algorithm for a stochastic process tree. Specifically, we define a two-dimensional array as a chromosome to represent a stochastic process tree, fitness function, a procedure for generating stochastic process tree and a model trace as a string of activities generated from the process tree. Furthermore, by storing and comparing model traces with low fitness values in the tabu list, we can prevent duplicated searches for process trees with low fitness value being performed. In order to verify the performance of the proposed algorithm, we performed a numerical experiment by using two kinds of event log data used in the previous research. The results showed that the suggested TS-GPM algorithm outperformed the GPM algorithm in terms of fitness and computation time.

Five-color bread was prepared by adding herb powder to Korean whole wheat triticum aestivum flour and the quality of baking was analyzed. The pH of the control bread was 6.15±0.12 while that of the whole wheat flour test group was 6.35±0.11, 6.29±0.12, 6.36±0.12, 6.19±0.11, and 6.01±0.13, respectively. The L value of the control bread was 67.78±0.03 and that of the whole wheat flour test group was 69.66±0.02, 60.01±0.12, 64.23±0.01, 63.34±0.01, and 61.64±0.04, respectively. The water activity was slightly increased at 2 days of storage due to the difference in water absorption and water retention. However, on the third day, the water transfer phenomenon in the bread showed water activity decrease in all wheat flour test groups. On the 1st day of the whole wheat flour test bread, the hardness values were 186.86±4.81, 165.89±3.73, 189.71±3.32, 198.38±2.19, and 184.29±3.40 g/cm2, respectively, and that of the control group was 138.84±3.72 g/cm2. The hardness of the control group and the whole wheat flour test group showed a significant difference. The internal structure of the bread in the whole wheat flour test group (100x, 500x) was not smoother than the control’s. The swelling degree of the starch particles and the cracking of the crumbs were confirmed by the adding of five-colored herbs to whole wheat flour.

This study was performed to evaluate the effect of heat stress on the status of physiological responses, blood parameter, serum T3 and cortisol, and heat shock proteins (HSP 27, 70, and 90) of Hanwoo cattle. Six Hanwoo steers (242.8 ± 7.2 kg of BW) were housed in the climate-controlled respiration chambers. The experiment consisted of 7 days (control; 0 day) at thermoneutral (air temperature (Ta) of 15oC and relative humidity (RH) of 60%; temperature-humidity index (THI) = 64), and by 3 and 6 days (treatment groups) at heat stress (Ta of 35oC and RH of 60%; THI = 87). Body temperature of each parts (frank, rump, perineum and foot) and rectal temperature elevated in heat stress groups (3 days and 6 days) than the control group (0 day). Respiration rates increased in 3 days and 6 days (88.5 ± 0.96 bpm and 86.3 ± 0.63 bpm, respectively) from 0 days (39.5 ± 0.65 bpm). Feed intake significantly decreased in heat stress groups (3 days and 6 days, 3.7 ± 0.14 kg and 4.0 ± 0.15 kg, respectively) than the control group (0 day, 5.0 ± 0.00 kg). In addition, final BW significantly decreased in heat stress groups (3 days and 6 days, 211.8 ± 4.75 kg and 215.5 ± 3.50 kg, respectively) than the control group (0 day, 240.0 ± 25.00 kg). However, heat stress has no significant effect on blood parameter, serum T3 and cortisol. Nevertheless, heat stress increased HSPs mRNA expression in liver tissue, and serum concentration of HSPs. Despite Hanwoo cattle may have high adaptive ability to heat stress, our results suggested that heat stress directly effect on body temperature and respiration rate as well as serum and tissue HSPs. Therefore, we are recommended that HSPs could be the most appropriate indicators of Hanwoo cattle response to heat stress.