Spent mushroom substrate (SMS) is a by-product remaining after a crop of mushrooms. The fresh SMS was sample within 24 hours of removal from the production facility in DOJUN farm located in Jinju. About 11 bacterial species were isolated from fresh SMS on TSA medium. Among of them, one isolate, designated YJ02, showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium, potentially. The strain YJ02 was produced cellulase, xylanase, mannanase as hydrolase. The strain YJ02 was identified as members of the genus Bacillus by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain YJ02 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus amyloliquefaciens with 16S rDNA gene sequence similarity of 98.7%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain YJ02 was classified within the genus Bacillus, for which the name Bacillus amyloliquefaciens YJ02 is proposed.

In Korea, twelve species in genus Myrmica have been described. Some of them are morphologically similar and this makes their identification difficult. For this reason, we collected several Myrmica species in question and inferred their phylogenetic relationship using the 418bp partial COI (cytochrome C oxidase 1) region from a total of 33 individuals. We found that the CO1 haplotypes are effectively grouped into three clusters that match well to their external morphological characters. Although this three species could be distinguished by the only small part of the COI region, the two individual sample of the M. kotokuii and one sample of the M. carinata are included in the M. kurokii group. The results indicate that the morphological identification could be obscure in the three species and it requires a close examination for this phenomenon.

Wolbachia is one of the most common endosymbionts best known to induce several reproductive alterations in its insect hosts. In some cases, the insect hosts harbor more than two strains of the bacterium. The Vollenhovia emeryi ant lives in dead trees and is morphologically subdivided into the long-winged and the short-winged. Interestingly the short-winged morph is free of Wolbachia, but only the long-winged morph is multiple- infected with the Wolbachia bacterium. We sampled four populations of the long-winged morph in Korea and performed pyrosequencing in Multilocus Sequence Typing (MLST), to determine the bacterial strain diversity. Six different gene regions (coxA, fbpA, ftsZ, gatB, hcpA and wsp gene) were targeted and amplified. However, the result shows that diversity of haplotypes is very high. The pyrosequencing approach in MLST, a new method of discriminating Wolbachia strains, is promising to effectively detect multiple infections and rare haplotypes.

After pine wilt disease (PWD) at Korean pine, Pinus koraiensis has been first reported in the middle area of South Korea in 2006, it spread rapidly to the nearest area. However, it is difficult to predict the spread of damage and set up the proper control strategy specific to P. koreaiensis because the pathology and the biology of PWD in P. koraiensis are not the same with PWD in P. densiflora and P. thunbergii distributed in middle southern area. So, we selected 25 of dead P. koraiensis in PWD damaged area and analyzed the inner-tree distribution of Bursaphelenchus xylophilus and the entrance holes of Monochamus saltuarius. There was no statistical significance between height or direction of wood extracted and the distribution of B. xylophilus among 25 dead pines. The entrance holes of M. saltuarius were concentrated on 7 dead pines without leaves and the maximum number of entrance holes in individual tree was 543. It indicates that the event of spawning of M. saltuarius adult and entrance of hatched M. saltuarius larvae to wood may be dependent on the time of death of host. The entrance holes were frequently found in a southward direction. These result shows that M. salutuarius larvae prefer relatively weak woody parts to enter easily. However, the relation between the thickness of bark of P. koraiensis and the frequencies of entrance holes of M. saltuarius was not observed.

Staphylococcus species are one of prevalent pathogens found in hospitals. Microbes that are a primary cause of nosocomial infection were isolated from a dental and medical environment it may assist the reader to explain what this is and how it differs from the ‘dental health care providers and ward health care providers’. To investigate the distribution of staphylococcus species in this environment, we used vitek II to measure drug sensitivity, and further performed biochemical testing. The isolation rate of staphylococcus species from the dental and medical environment was 100% but from dental health care providers and ward health care providers were 44.4% and 33.3%, respectively. In the analyses, staphylococcus species showed resistance to diffusion of cefoxitin and oxacillin discs. These staphylococci may be sufficiently positive for the mecA gene. Our results suggest that staphylococci might be an important cause of nosocomial infection in the dental clinic.

The influence of the culture media on the growth and catechins and caffeine production was investigated in adventitious root cultures of tea tree. The growth rate of adventitious roots was higher than that of solid medium. Growth rate of adventitious roots was noted to be optimal in N6 liquid medium. Yields of (-)-epicatechin gallate (ECG) and caffeine were the highest when adventitious root cultures were maintained in Nitsch and B5 liquid medium, respectively. The production of (-)-epicatechin (EC) and (-)-epigallocatechin gallate (EGCG) from adventitious roots was maximal when cultured in 1/2MS liquid medium. The adventitious root extracts of tea tree produced on catechins as EC (20.77 mg/g) and EGCG (1.84 mg/g) in 1/2MS medium; EGC (24.39 mg/g) and caffeine (12.97 mg/g) in B5 medium and (-)-epigallocatechin (ECG) (2.16 mg/g) in Nitsch liquid medium.

The mushrooms have been used as traditional medicines and food resources in many countries. The objective of this study was to determine antioxidant compounds and to evoluate tyrosinase inhibitory activity of extracts from Hypsizigus marmoreus. The pileus and stipe of Hypsizigus marmoreus were extracted with methanol and water, separately. The methanol extract of Hypsizigus marmoreus were fractioned by hexane, chloroform, ethylacetate and buthanol. The concentrations of total polyphenolics and flavonoids in methanol extracts were investigated by colorimetric methods. The concentrations of total polyphenolics and flavonoids in methanol extract of the pileus was higher than methanol extract of the stipe. The DPPH redical scavenging activity of the pileus extract was also higher than stipe extract in methanol extract. The IC50 of DPPH redical scavenging activity of the pileus and stipe in methanol extract were 18 mg/ml and 1 10 mg/ml, respectively. The IC50 of tyrosinase inhibitory activity of the pileus and stipe in methanol extract were 500 mg/ml and 1,000 mg/ml in methanol extract. These results suggest that Hypsizigus marmoreus can be potentially used as a source of natural antioxidant agent in the cosmetic industry as well as the food, pharmaceutical and medicinal industry.

This paper deals with a planning problem for reentrant hybrid flow shop with serial stages consisted of parallel machines. In a reentrant operation, a product may revisit some stages several times and then it may cause the congestion of work in process (WIP) or equipment idleness. Preemptive goal programming based heuristics are proposed to maximize of throughput and to minimize of delayed customer demands in the hybrid flow shop.

Sweet potato whitefly (SPW), Bemisia tabaci, is the most serious pest of greenhouse tomato as a vector of tomato yellow leaf curl virus since 2008 in Jeju. This study was conducted for within-plant distribution of SPW nymphs on leaves and the characteristic of spatial distribution of SPW adults using by yellow-color sticky trap in greenhouse-grown tomato plants. The nymphs of SPW were mainly distributed on leaves of mid-lower stratum in three stratum of tomato plant. They were also more distributed on three to four leaflets from basal part in a leaf. Ten plots and three plants per plot were appropriated to estimate of mean density of SPW nymph using by visual inspection in a tomato greenhouse. The more SPW adults were caught on horizontally placed sticky trap above 50～80 cm than vertically placed sticky trap above tomato canopy. The horizontal direction trap should be placed in opened site. SPW adults were shown the aggregated distribution pattern using by Taylor’s power law (TPL) and Iwao’s patchiness regression (IPR) model which are commonly used for spatial distribution pattern analysis. TPL was showed better description than IPR for spatial distribution pattern of SPW adults. The required number of trap tended to decrease according to increase the mean density of SPW adult

Vollenhovia emeryi ant is distinguished by its wing morphology; short winged (SW) and long winged (LW). Its reproduction shows a bizarre genetic caste system distinct from other social hymenopteran insects. Unfertilized eggs undergo genome duplication and develop into clonal gynes. Fertilized eggs develop either into workers or males. The fate of the fertilized eggs is determined whether maternal genome loss (MGL) takes place after fertilization. Eggs with MGL become haploid males with only paternal half of the genome. Without MGL, the eggs become workers with maternal and paternal half of the genome. In this research, we analysed 5 nuclear genes of SW and LW individual ants. Among them, two genes from an SW male are identical to those of LW, and one gene from the SW male seems a variant of LW. The result indicates that SW males are derived from LW colonies. From the genetic relatedness point of view individuals in the same castes are genetically identical. On the other hand, between workers and two reproductives, the relatedness is asymmetrical and there is even no gene sharing between gynes and males. The conventional genetic relatedness by Hamilton is revised under this condition.

In order to isolate thermophilic compost-promoting bacteria with high activity of cellulase and xylanase, spent mushroom substrates with sawdust were collected from mushroom cultivation farm, Jinju, Gyeongnam in Korea. Among of the isolates, one strain, designated HJ01 was selected by agar diffusion method. The strain HJ01 was identified as members of the Bacillus subtilis by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain HJ01 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rDNA gene sequence similarity of 99.3%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain HJ01 was classified within the genus Bacillus, for which the name Bacillus subtilis HJ0 is proposed. The cellulase and xylanase activity of B. subtilis HJ0 was slightly increased according to bacterial population from exponential phase to stationary phase in growth curve for B. subtilis HJ01.

Spent mushroom substrate(SMS) is a by-product remaining after a crop of mushrooms. About 9 thermophilic strains were isolated from SMS(Flammulina velvtipes). Among of them, one isolate, designated UJ09, showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium, potentially. The strain UJ09 was produced cellulase and xylanase as hydrolase. The strain UJ21 was identified as members of the genus Bacillus by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain UJ09 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus amyloliquefaciens with 16S rDNA gene sequence similarity of 99.2%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain UJ09 was classified within the genus Bacillus, for which the name Bacillus amyloliquefaciens UJ09 is proposed.

Beet armyworm (BAW), Spodoptera exigua (Hübner), is a polyphagous lepidopteran pest mainly distributed in tropical and subtropical regions. BAW are known to migrate long distances to find suitable host plants and environmental condition. The possibility of its overwintering in temperate regions has been suspected. This study was conducted for investigating overwintering and phenology of BAW using by sex pheromone trap in Jeju during 2007 to 2011. BAW was able to overwinter at pupae stage regardless of temperature of winter season in Jeju. The times of BAW caught first on traps were different by surveyed years - early March in 2007, middle March in 2008 and 2011 and early April in 2009 and 2010. The times of BAW caught on trap tended to be more dependent on temperature variation during on November and December than on mean temperature of winter season. The peak season of adults of the overwintering generation was early to middle of May. The results of seasonal periodicity of BAW monitored by sex pheromone trap during 2009 and 2010 indicated that five generations were passed in Jeju. The peak season of 1st generation was on middle of April, 2nd generation on middle of July, 3rd generation on early of August, 4th generation on late of August and 5th generation on middle of September. There were similar patterns by different above sea levels except the season of overwintering generation adults occurrence (March to May).

Vollenhovia emeryi chosenica (Wheeler) (Hymenoptera: Myrmicinae) is an ant species frequently found in forests. In nature, two phenotypically distinct forms are found e.g. long winged and short winged. Unlike other hymenopteran insects, the ant is unique in its mode of reproduction. In this species, queens are clonally reproduced from unfertilized eggs. On the other hand, workers develop from fertilized eggs. Strikingly, haploid males are reproduced from fertilized eggs after destroying the maternal half of the genome e.g. maternal genome loss (MGL) consequently only with the paternal half of the genome. We collected the ant colonies nationwide in 2011. In this study, we demonstrate that the ant is infected with Wolbachia, the bacterial reproductive manipulator in various insects. Interestingly, only the long winged morphs seem to be infected. Furthermore, most colonies are mulitple-infected except two colonies collected from Chuncheon and Mt. Deogyu. We will discuss potential interactions among the Wolbachia infection polymorphism and wing morphology, and evolution of clonal reproduction and MGL.

The black-veined white, Aporia crataegi (Lepidoptera: Papilionoidea), is nearly extinct in South Korea, although substantial numbers of dried specimens are available. One of the common practices for such species is to launch re-introduction program after proper amount of genetic information are analyzed from donor and donee populations. In this study, we sequenced complete mitochondrial genome (mitogenome) of A. crataegi to design species-specific primers for subsequent population works and to further understand the mitogenome evolution in lepodiopteran Papilionoidea. The 15,140-bp long A. crataegi mitogenome that has typical sets of 37 genes is smallest among true butterfly species with overall slightly smaller size in genes and regions throughout the genome. Arrangement of the genome is identical to those of other lepidopteran mitogenomes, in which tRNA cluster located between the A+T-rich region and ND2 gene is translocated into tRNAMet, tRNAIle, and tRNAGln from ancestral arrangement, tRNAIle, and tRNAGln, tRNAMet. The A/T content of the genome at 81.3% is the highest in Pieridae, but lower than that of lycaenid species (81.7% ~ 82.7%) The high A/T content in the genome is also reflected in codon usage, accounting for 41.69% of A/T-composed codons (TTA, ATT, TTT, and ATA). Unlikely the diversified or modified usage of anticodon for tRNASer(AGN) the species of Pieridae including A. crataegi all unanimously have GCT that has been hypothesized as ancestral for Lepidoptera. A total of 111 bp of non-coding sequences are dispersed in 13 regions, ranging in size from 1–49 bp. Among them relatively longer ones (≥ 16 bp) all have relatively higher sequence identity to other regions of the genome, suggesting partial duplication of the sequences during A. crataegi evolution. As has been reported in some species of Lepidoptera, the A. crataegi A+T-region also has typically found conserved sequences (e.g., poly-T stretch, ATAGA motif, ATTTA element, microsatellite-like A/T sequence, and poly-A stretch) and one tRNA-like sequence, and this feature was commonly found in true butterfly species.

The gene (2,304-bp) encoding a novel xylanolytic enzyme (XylD) with a catalytic domain, which is 70% identical to that of Cellulomonas flavigena DSM 20109 GH6 β-1,4-cellobiohydrolase, was identified from an earthworm (Eisenia fetida)-symbiotic bacterium, Cellulosimicrobium sp. strain HY-13. The enzyme consisted of an N-terminal catalytic GH6-like domain, a fibronectin type 3 (Fn3) domain, and a C-terminal carbohydrate-binding module 2 (CBM 2). XylDΔFn3-CBM 2 displayed high transferase activity (788.3 IU mg-1) toward p-nitrophenyl (PNP) cellobioside, but did not degrade xylobiose, glucose-based materials, or other PNP-sugar derivatives. Birchwood xylan was degraded by XylDΔFn3-CBM 2 to xylobiose (59.2%) and xylotriose (40.8%). The transglycosylation activity of the enzyme, which enabled the formation of xylobiose (33.6%) and xylotriose (66.4%) from the hydrolysis of xylotriose, indicates that it is not an inverting enzyme but a retaining enzyme. The endo-β-1,4-xylanase activity of XylDΔFn3-CBM 2 increased significantly by approximately 2.0-fold in the presence of 50 mM xylobiose.

Arrowhead scale, Unaspis yanonensis, is a serious scale insect pest of citrus in Jeju. The sticky taping of 1.0 cm diameter twig was a good survey method for arrowhead scale crawler. There are three times crawler occurrence season - late May to early July, late July to middle September and late September to late October. But the third occurred crawlers are all died during overwintering in open-field groves. This study was conducted to provide information on efficient sampling plan for arrowhead scale within the tree. Twig and fruit was fit for secondary sample unit by two-stage variance analysis. Both sample number per tree (primary sample unit) was eight twigs or fruits. Taylor’s power law was better described the distribution characteristic of arrowhead scale than Iwao’s patchiness regression. The slope of both linear regressions was greater than “1” which means aggregative distribution pattern. Minimum sample size to estimate the density was calculated using by two parameters of Taylor’s power law. To estimate the mean density on twig and fruit by binomial sampling plan, the optimum tally threshold was 5 and 7, respectively.