Structural and mechanical effects of the dynamical precipitation in two copper-base alloys have been investigated over a wide range of deformation temperatures. Basing upon the information gained during the experiment, also some general conclusion may be formulated. A one concerns the nature of dynamic precipitation(DP). Under this term it is commonly understood decomposition of a supersaturated solid solution during plastic straining. The process may, however, proceed in two different ways. It may be a homogeneous one from the point of view of distribution and morphological aspect of particles or it may lead to substantial difference in shape, size and particles distribution. The effect is controlled by the mode of deformation. Hence it seems to be reasonable to distinguish DP during homogeneous deformation from that which takes place in heterogeneously deformed alloy. In the first case the process can be analyzed solely in terms of particle-dislocation-particle interrelation. Much more complex problem we are facing in heterogeneously deforming alloy. Deformation bands and specific arrangement of dislocations in form of pile-ups at grain boundaries generate additional driving force and additional nucleation sites for precipitation. Along with heterogeneous precipitation, there is a homogeneous precipitation in areas between bands of coarse slip which also deform but at much smaller rate. This form of decomposition is responsible for a specially high hardening rate during high temperature straining and for thermally stable product of the decomposition of alloy.

‘Morning Bell’ 품종은 생육이 왕성하고 엽소 및 잎마름병에 강하며 꽃이 큰 백합 신품종을 육성하기 위하여 2008년 충남 농업기술원 태안백합시험장에서 교배한 OT(Oriental hybrid× Trumpet hybrid)계통의 종간교잡종이다. 모본으로는 복색(백 색+분홍색)의 OT계통 ‘Altari’ 품종을, 부본으로는 분홍색의 Oriental계통 ‘Solaia’ 품종을 화주절단수분법에 의해 교배한 후 2009년부터 2012년까지 기내 종자배양 및 증식 육묘하여 2013년 우수계통(OT13-9)을 1차 선발하였다. 2013년부터 2015년도에 걸쳐 ‘Candy Club’ 품종을 대조구로 하여 보통재배를 통한 특성검정을 실시하였으며, 2014년 화훼연구소에서 개최 한 신품종 평가회에서 기호성이 우수한 개체를 2차 선발하여 ‘Chungnam OT-3’로 명명한 후 농촌진흥청 농작물직무육성 신품종선정위원회 심의를 거쳐 국립종자원에 ‘Morning Bell’로 품종보호 출원하였다.‘Morning Bell’ 품종의 중부지역 자연 개화는 7월 2일로 ‘Candy Club’ 품종과 비슷하였고 초장은 120.8cm이었다. 진분홍색 반점에 복색(크림색+적색)의 화색과 상향개화성이었다. 잎마름병과 진딧물 등의 병충해 방제를 주기적으로 실시하고, 양수분 관리 등 일반적인 재배관리를 통해 고품질 절화 및 구근생산이 가능하다.

‘Golden Bell’ 품종은 생육이 왕성하고 엽소 및 잎마름병에 강하며 꽃이 큰 백합 신품종을 육성하기 위하여 2007년 충남 농업기술원 태안백합시험장에서 교배한 OT(Oriental hybrid× Trumpet hybrid)계통의 종간교잡종이다. 모본으로는 노랑색의 OT계통 ‘Yelloween’ 품종을, 부본으로는 백색의 Oriental계 통 ‘Muscadet’ 품종을 화주절단수분법에 의해 교배한 후 2008년부터 2010년까지 기내 종자배양 및 증식 육묘하여 2011년 우수계통(OT11-2)을 1차 선발하였다. 2012년부터 2013년도에 걸쳐 ‘Yelloween’ 품종을 대조구로 하여 보통재배를 통한 특 성검정을 실시하였으며, 2012년 화훼연구소에서 개최한 신품종 평가회에서 기호성이 우수한 개체를 2차 선발하여 ‘Chungnam OT-4’로 명명한 후 농촌진흥청 농작물직무육성 신품종선정위 원회 심의를 거쳐 국립종자원에 ‘Golden Bell’로 품종보호 출원하였다.‘Golden Bell’ 품종의 중부지역 자연개화는 6월 16일 로 ‘Yelloween’ 품종과 비슷하였고 초장은 136.4cm이었다. 반점이 없는 노랑색의 화색과 상향개화성을 나타내었다. 재배에 있어서는 잎마름병과 진딧물 등의 병충해 방제를 주기적으로 실시하고, 양수분 관리 등 일반적인 재배관리를 통해 고품질 절화 및 구근생산이 가능하다.

Few studies have reported how temperature influences growth and development of succulents, including anthocyanin production, which could fetch better prices in the market, and understanding the factors influencing such pigments would benefit farmers. The present study investigated the effect of temperature (10°C, 20°C, and 30°C) on the growth, development, and anthocyanin concentrations in Echeveria agavoides and E. marcus. In E. agavoides, similar growth performance was observed at 10°C and 20°C based on plant height and diameter. However, subjecting the species to a high temperature of 30°C resulted in a decrease in plant height. In E. marcus, optimal growth performance was observed at 20°C. Different temperatures did not significantly affect succulent quality and color hues. Only L* values were significantly different among the Hunter’s Lab values. Similar results were observed following anthocyanin and image analyses, both of which were not significantly affected by temperature. However, an intense red pigment was observed at 20°C compared with the green pigment observed at 10°C and 30°C based on the image analysis. The results suggest that temperature influences growth, development, and anthocyanin content of Echeveria succulents, and 20°C could be the optimal temperature for the cultivation of the species.

Chamaecyparis obtusa is one of the economical conifers planted in Korea due to its good quality timber and wood characteristics. Individuals of C. obtusa containing high terpenes (HT) and low terpenes (LT) were selected for by colorimetric method. The HT of C. obtusa was delayed in wilting against various abiotic stresses compared to the LT plants. The HT group exposed to UV did not significant influence the chlorophyll content, and the chlorophyll value was higher in the HT group than the LT group. Also, chilling treatment (5℃) did not significant influence on the chlorophyll content. However treatment at -4℃ showed relatively low chlorophyll content in the LT group than the HT group. Plants exposure to high temperature was not a difference between the HT and the LT group. However, treatment at 38℃ influenced the chlorophyll content that was increased exposure time-dependently. In salt treatments, chlorophyll in the HT group was lower at high concentrations (300 and 500 mM) of NaCl. However, chlorophyll content increased to slightly in treatment time-dependently, which is 6.7% to 40%. H2O2 treatment has been a negative effect on the chlorophyll content in the HT group. All concentration of H2O2 decreased the chlorophyll content of 5% to 35%. Plants containing high terpenoids were resisted against some abiotic stress such as salt and H2O2. Our results implied that terpenoids could cause various abiotic stress resistance. These results could be utilized for efficient management and biomass production during forest silvicultures.

Stem and root of elm trees have used as traditional medical materials, but there is little information on the distribution and resources of habitats. Korean native growing Ulmus spp. (U. davidiana var. Japonica, U. parvifolia, U. davidiana, and U. macrocarpa) genetic resources studied through The National Forest Inventory of Korea data and field survey. The distributions of U. davidiana var. japonica according to elevation distributed evenly. Both U. parvifolia and U. davidiana were inhabited mostly at less than 200 m of altitude. Each Ulmaceae species widely were distributed nationwide, but a dominant species was different depending on locals. It observed that Ulmaceae inhabits mainly in steep slopes of 31-45 degrees. Most of the habitats regenerated by natural seeding and the most abundant species were a codominant tree. Distribution of trees in U davidiana var. japonica was 7 m-13 m, and in young U. parvifolia and U. macrocarpa, more than 25% of young trees less than 7 m observed. The distribution of the diameter of breast height of the U. davidiana var. japonica was 46.4% for 11-20 cm, 52.6% for 11-20 cm in U. parvifolia. The average T/R ratio was 0.83, and the mean weight ratio of root bark was 62%. As the results of this study, the domestic Ulmaceae biomassare very small. It is difficult to harvest in that the habitat on the slope. Thus, it is too hard to develop functional materials using biomass at present. Therefore, it is necessary to develop technology for the selection and propagation of elite trees of Ulmaceae.

This study was conducted to evaluate the efficacy of mulberry cake mixed diet on larval growth of Protaetia brevitarsis. As a result of adding mulberry cake to oak fermented sawdust and mulberry fermented sawdust, the survival rate of P. brevitarsis larvae were higher than those of the control when mass rearing larvae. When fed oak fermented sawdust with 3, 5% and 10% of mulberry cake, the cumulative proportion of larvae over 2.5g was 92.9% at 65 days, 90.8% at 55 days, and 96.3% at 45 days after rearing at 25±1℃, respectively. When fed mulberry fermented sawdust with 3%, 5% and 10% of mulberry cake, the cumulative proportion of larvae over 2.5g was 86.2% at 85 days, 90.7% at 75 days, and 91.5% at 75 days after rearing at 25±1℃, respectively.

This study was conducted to evaluate the efficacy of mulberry leaf mixed diet on larval growth of Protaetia brevitarsis. As a result of adding mulberry leaves to oak fermented sawdust, the survival rate of P. brevitarsis larvae were higher than those of the control when mass rearing larvae. When fed oak fermented sawdust with 5%, 10% and 20% of mulberry leaves, the cumulative proportion of larvae over 2.5g was 93.1% at 65 days, 95.6% at 55 days, and 93.9% at 55 days after rearing at 25±1℃, respectively. Also, heavy metals were not detected in larvae of P. brevitarsis fed on mulberry leaves.

Here, we investigated antioxidant defense mechanism in the spermatheca of A. mellifera queens via RNA-seq analysis of spermathecae in both mated and virgin queens. We identified the genes encoding antioxidant proteins, which were differentially expressed in the spermatheca of mated queens. The concentrations of antioxidant proteins, such as superoxide dismutase 1 (SOD1), catalase, glutathione peroxidase (GTPX), and transferrin (Tf) together with the levels of ROS, H2O2, and iron were higher in the spermathecal fluid of mated queens as opposed to those in the spermathecal fluid of virgin queens; this indicated that increase in antioxidant protein concentration is an antioxidant defense mechanism occurring in the spermathecal fluid of mated queens against ROS; this mechanism involves conversion of ROS using antioxidant enzymes and Tf-mediated inhibition of the Fenton reaction occurring between Fe2+ and H2O2. Our data indicate that an increased expression of antioxidant proteins could facilitate prolonged storage and survival of sperms in the spermatheca of mated queens, suggesting the role of antioxidant proteins in antioxidative defense against ROS.

South Korea has over 0.38 million of managed honey bee (Apis cerana) colonies before 2009 years ago, which produce the highest quantity of honey in the Korea; however, almost colony (90%) were collapsed by Korean Sacbrood Virus (KSBV) in South Korea. Korean Sacbrood Virus (KSBV) is the pathogen of A. cerana Sacbrood disease, which poses a serious threat to honeybee A. cerana, and tends to cause bee colony and even the whole apiary collapse. Colony collapse of A. cerana was first reported on the Pyeong-Chang of the South Korea in 2009. Several scientists and governments has been tried research for cure the sacbrood disease in A. cerana colony by medicines and management techniques. Unfortunately, The sacbrood disease dosen’t improve. So, we were developed a better breed of A. cerana for resistance of sacbrood virus by selection and then artificial insemination. A. cerana breeding technique was first successful applied with A. cerana in Korean. Queens was grafted from sacbrood resistance line and then it was growing in sacbrood disease colony that was survived 100%. Altogether selected 18 queens were artificially inseminated and 2,000 drones of A. cerana in Korea was used to evaluate amount of semen collection. We are select two scabrood resistance A. cerana line (R and H). R line be used for rearing the Queen. Drone was reared in H line colony. The RH hybrid were not infected sacbrood virus even spread sacbrood virus (2×106). RH colonies have very excellent hygienic behavior, brood, and sacbrood disease resistance activity.

In order to investigate novel insect growth regulators (IGRs) from actinomycetes, 363 isolates of actinomycetes were tested for their IGR activities. Among them, Streptomyces sp. AN120537 showed the highest juvenile hormone antagonist (JHAN) activity and significant insecticidal activities against larvae of Aedes albopictus and Plutella xylostella. In addition, the dead larvae showed morphological deformities such as contraction of body segments or pigmentation of all body parts. Through liquid chromatography and bioassay-guided fractionation, 7 IGR compounds were identified from the crude hexane extract of AN120537. These results suggested that Streptomyces sp. AN120537 could be useful resources for development of eco-friendly insecticidal agents.

The periodic occurrence of biological population is affected by several biological and environmental factors and ancient literature survey offers insight into ecological process related to the cyclicity. The periodic occurrence of a Lepidopteran species, the pine caterpillar (Dendrolimus spectabilis), was estimated based on ancient literature survey. The pine caterpillar was one of the most injurious defoliators of pine trees in northeast Asia, especially Korea during 1960s and 1970s. Outbreaks of this species have long history in Korea. Since about 900 years ago, damages of pine forest by this species and its control were recorded both in History of Corea dynasty which cover 475 years (918-1392) of the history of the Corea Dynasty and in the Annals of Joseon Dynasty (Joseon Wangjo Shillok) which cover 472 years (1392-1863) of the history of the Joseon Dynasty, respectively. At least over 20 and 30 occurrences of pine caterpillar were recorded during Corea dynasty and Joseon Dynasty, respectively. These results showed that the pine caterpillar had been one of the most severe forest pests during Corea and Joseon Dynasty. Our result showed that the ancient literature survey is useful to understand the long-term periodic occurrence of the pine caterpillar.

This study was conducted aiming to figure out two things. First, knowledge of the change in spatial distribution of Tetranychus urticae depending on how to control it (using pesticide or natural enemy). Second, spatial association of T. urticae and Phytoseiulus persimilis in biocontrol plot (B.P). The data was analyzed by spatial analysis by distance indices (SADIE) using global aggregation index, Ia . Ia values were 0.77-1.37 in conventional plot (C.P) and 0.88-1.68 in B.P, respectively. However, the fluctuation level of Ia in B.P was higher than C.P. Therefore, the results indicated that there was a clear spatial pattern change in B.P, i.e. prey’s spatial distribution is affected by natural enemy. And spatial association analysis showed that T. urticae and P. persimilis have positively associated. It means that T. urticae is relatively low mobile prey, and P. persimilis is relatively high mobile predator.

Two infamous ant species, red imported fire ant (RIFA, Solenopsis invicta) and tropical fire ant (TFA, S. geminata), originated from South America are invading and harming many countries around the world. Among them, RIFA, known as one of the 100 world’s worst invasive alien species, has been discovered in South Korea; in Busan (Gamman CT) in 2017, and subsequently in Busan (Hutchison CT), Pyongtaek, Incheon and Daegu in 2018. With these relentless introduction of RIFA, South Korea is not safe no more from these ants and faces a risk of settlement. Our research goal is tracing the country origin of invasive populations of RIFA and TFA based on molecular epidemiological approach. We are conducting both microsatellite and genome-wide SNP analyses in order to derive a complementary interpretation. These two methodologies are expected to result in improved resolution in population genetics analysis. Nevertheless, this work needs collaboration of many countries belonging to the range of origin and invasion of RIFA and TFA. We will briefly introduce our research project and progress of collaboration research.

고온기 화색발현이 우수하고 연중생산이 가능한 수출용 스프레이국화 신품종을 육성하기 위하여 충남농업기술원 화훼 연구소에서 2010년 분홍색의 모본 ‘Borami’를 방임수분하였다. 2011년에 종자를 파종하였고, 이중 화형과 화색이 우수한 개체를 선발하여 ‘SP11-148-01’로 계통명을 부여하였다. 2011년부터 2013년까지 주년 생산성을 위해 전조, 자연, 차광재배로 특성을 각각 검정하였고, 생육 및 개화특성은 화형과 화색이 비슷한 자주색 스프레이국화인 ‘Kingfisher’를 대조품종으로 조사하였으며, 2013년 ‘Yes Ruby’로 품종등록 출원하였다. ‘Yes Ruby’는 자주색의 설상화와 연녹색의 통상화로 가을 작형 개화기는 10월 24일로, ‘Kingfisher’의 10월 29일에 비해 빨랐다. ‘Yes Ruby’의 초장과 줄기굵기는 각각 94.9cm와 7.7mm로 ‘Kingfisher’의 89.2cm와 6.4mm보다 컸다. ‘Yes Ruby’의 꽃 직경은 6.2cm로 ‘Kingfisher’의 5.0cm보다 컸으며, 꽃잎수도 ‘Yes Ruby’가 25.7개로 ‘Kingfisher’의 23.3개보다 많았다. 착화수는 두 품종 모두 비슷하였으며, 흰녹병 저항성은 ‘Yes Ruby’가 2단계, ‘Kingfisher’는 3단계의 감염 정도를 나타내어 흰녹병에 대한 저항성이 높은 것으로 나타났다. 재배상 유의사항은 ‘Yes Ruby’는 겨울철 균일한 개화가 이뤄지지 않아 겨울철 야간온도를 18℃ 이상으로 관리해줌으로써 균일개화를 유도할 수 있다. 또한 생장억제제인 Daminozide을 처리함으로써 설상화수를 늘려 볼륨감 높은 꽃봉오리를 형성할 수 있어 고온기에도 화색발현이 우수하고 연중생산이 가능하여 안정적 수출을 통한 농가소득 증대에 기여할 수 있을 것으로 기대된다.

아보카도는 에콰도르에서 경제적으로 중요한 과수이다. 건강한 종묘 생산은 아보카도 식물의 생산에서 중요한 단계이며 유익한 미생물의 처리는 식물체의 영양을 향상 시키는데 사용 될 수 있다. 아보카도 묘목에 처리한 미생물의 효과를 검정한 결과, 묘목의 뿌리부분과 지상부의 양분 흡수를 증가시켰다. T. harzianum은 뿌리에서 N과 Mg의 흡수를 유의적으로 증가시켰으며, 지상부에서는 N, Ca, Mg, Mn 및 Cu의 흡수를 증가시켰다. 반면에, G. iranicum var tenuihypharum은 뿌리에서 Ca와 Fe의 흡수를 유의적으로 증가시켰으며, 지상부에서는 N흡수를 증가시켰다. 또한, mycorrhiza의 접종으로 아보카도 묘목의 뿌리 부분과 지상부에서 P 양을 증가시키는 경향이 긍정적으로 나타났다. 두 미생물 모두 S의 흡수에 영향을 미치지 않았는데, 이는 식물 전체에서 균일한 비율을 나타냈다. 또한 인산질 비료(일인산 칼륨)의 시용은 미량 영양소의 흡수에 긍정적인 영향을 미쳤다.

Photosynthetic characteristics and growth responses of Phalaenopsis Queen Beer ‘Mantefon’ orchid were determined in plants exposed to variable carbon dioxide (CO2) concentrations at 2-, 24-, and 36-weeks age (i.e., corresponding to juvenile, young, and mature vegetative growth stages, respectively). Plants were grown at 400 (control), 800, or 1,600 μmol･mol-1 CO2 for 6 hours during the nighttime for 32 weeks. Phalaenopsis ‘Mantefon’ in 2- and 24-week-old plants grown at 1,600 μmol･mol-1 CO2 had increased leaf number and net CO2 uptake compared with the plants grown at 400 μmol･mol-1 CO2. In 36-week-old of Phalaenopsis ‘Mantefon’, leaf number was significantly greater in plant grown at 800 and 1,600 μmol･mol-1 conditions compared with plants grown at 400 μmol･mol-1 CO2. Leaves that emerged after the start of the CO2 treatment were initially longer in the plants grown at 1,600 μmol･mol-1 CO2 than at 400 μmol·mol-1 C O2, but the final leaf length was shortest in the plants grown at 1,600 μmol･mol-1 CO2 condition. Plants showed crassulancean acid metabolism characteristic of nighttime CO2 uptake regardless plant growth stages. We found that growers may be able to promote leaf growth with increasing leaf number and reducing time to leaf initiation in the 36-week-old (i.e., mature stage) plants with 800 – 1,600 μmol·mol-1 CO2 and 2- and 24-week-old (i.e., juvenile and young stages) plants with 1,600 μmol·mol-1 C O2 for Phalaenopsis ‘Mantefon’.

Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to 3 μM or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, 2 μM of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.

본 연구를 통해 잔디주머니나방속이 국내에서 처음 발견되어 보고한다. 잔디주머니나방속내 잔디주머니나방에 대한 성충, 유충, 번데기 등 형태학적 특징을 재기재하였다. 또한 신속한 종 동정을 위한 DNA바코드 정보를 제공하였다.