High oleate oil enhance flavors and shelf life and decrease coronary heart disease mortality. A new peanut(Arachis hypogaea ssp. fastigiata L.) variety “K-Ol(Milyang56)” with high oleic acid of fatty acid composition was developed at the Department of Functional Crop, NICS, in Milyang in 2013. This variety was developed from the crossing line between cultivar “Baekseon” with large grain and erect plant type and “F435-5” with high oleate and runner type. “K-Ol” which is Shinpung plant type has 43cm of main stem length and 10 branch number per plant. This also show more resistant to late leaf spot, web blotch and stem rot, compared with check variety “Daekwang”. Each pod has two grains with short ellipse shape of brown testa and yield components is composed of 46 mature pods of per plant, 74g of 100-seed weight, 76% of pod shelling ratio in the regional yield trials(RYT). For 3 year regional yield trials the average yield of “K-Ol” had 4.45 MT/ha for kernel similar to that of check variety. Seed quality show 50.7% of crude oil and 82.9% of oleic acid. The oil acidification of “K-Ol” measured by acid value(P-A value) variation for 3 hours at 180 ℃ showed one-ninth as low as ‘Daekwang’ do. This variety is expected to develop new demand area such as the improvement of cardiovascular disorders and shelf-life, and import substitution of olive oil.

A new peanut(Arachis hypogaea ssp. fastigiata L.) variety “Danwon(Milyang51)” with short stem length and lodging resistance was developed at the Department of Functional Crop, NICS, in Miryang in 2013. This variety was developed from the crossing line between cultivar “YG 75” with large grain and fewer branch number and “SP9617” with short stem length. “Danwon” which is Shinpung plant type has 32cm of main stem length and 13 branch number per plant. Each pod has two grains with long ellipse shape of brown testa and yield components is composed of 40 mature pods of per plant, 91g of 100-seed weight, 77% of pod shelling ratio in the regional yield trials(RYT). Seed quality show 48.7% of crude oil and 26.9% of protein content. “Danwon” has resistance to lodging in even double dense planting without yield reduction. This is more resistant to early and late leaf spot, web blotch and stem rot, compared with check variety “Daekwang”. In the regional yield trials “Danwon” outyielded check variety by 6% with 4.52 MT/ha for kernel.

본 연구는 땅콩 파종후 발아기의 내습성 기초자료를 얻고자 파종일수와 침수처리에 따른 출현율을 조사하고 적정 내 습성 검정방법을 찾고자 하였으며 이를 이용하여 품종별 내 습성 차이를 구명해보고자 본시험을 수행하였다. 종자의 흡수율 50%에 도달한 것은 공히 5시간 정도 소요 되었으며 24시간 후에는 조광 66.6%, 대광 65.1%, 팔광 67.1%에 도달하여 품종간 큰 차가 없었다. 땅콩종자를 vermiculite 풋트에 파종한 후 바로 침수시켰을 때 대광땅콩 과 팔광땅콩은 공히 침수 16일까지도 90% 정도의 출현율 을 유지하였고 그 후로는 급속히 감소하여 침수 25일에는 출현율이 거의 없었다. 파종 후 발아일자 1~4일에 각각 침 수일자 1~5일간 침수처리를 하였을 때 발아일수가 길어질 수록, 침수일자가 길어질 수록 땅콩의 출현율이 급속히 떨 어졌다. 대광땅콩은 파종 1~3일 후 2일 침수처리까지, 팔광 땅콩은 파종 1~2일 후 2일 침수처리까지 정상 출현하였으 며 두 품종 공히 3일 이상 침수에서는 모든 발아일수(1~4 일)에서 출현율이 급속히 떨어지고 있었다. 파종 2일 후 3일간 침수처리 방법에 따라 국내육성 31품 종에 대해 침수 시 내습저항성을 검정한 하였을 때 출현율 은 0에서 69%까지 분포하였고 대광땅콩 가장 높았다.

간식용 대립 땅콩의 선호도가 높음에 따라 단경 대립 다수 성 신품종 육성을 위하여 대립 다수성인 ‘남광’과 ‘U2-12-6’ 의 교잡계통과 단경 품종인 ‘사도노카’를 인공교배하여 육성 한 ‘연풍’의 주요 특성을 요약하면 다음과 같다. ‘연풍’은 버 지니아 초형으로 개화기가 빠르고 지상부 생장습성은 단경다분지성이다. ‘연풍’은 ‘대광’에 비해 성숙협수, 협실비율, 100협립수, 100립중등 양호한 수량구성요소를 보이며 100립 중이 88 g인 대립품종이고, 도복은 매우 강한 특성을 나타내 며 흑반병, 그물무늬병에 비교적 강하고 낙엽율도 낮아 생육 후기까지 녹엽 유지에 유리하였다. ‘연풍’은 단백질이 31.9%, 기름함량이 49.8%이고 지방산은 올레산과 리놀산의 비율이 같은 특성을 가지고 있다. ‘연풍’은 3년간 지역적응시험 결과 ‘대광’에 비하여 전국 평균 4.81 ton로서 15% 증수되었다. ‘연풍’은 경장이 짧고 병해에도 강한 편이나 충분한 성숙을 위하여 경기북부와 강원도, 산간 고냉지를 제외한 지역에서 적응성이 높은 품종이다.

‘보원’은 단경, 소분지 계통인 ‘SP8603-16-2-B(찌바한다치 /대광땅콩)’를 모본으로 하고 내병, 대립 특성을 가지는 대원 땅콩을 부본으로 하여 2001년도에 작물시험장에서 육성한 품 종이다. ‘보원’은 신풍초형(Arachis hypogaea ssp. fastigiata) 으로 키가 작고 지상부 생육 습성이 완전 직립형이다. 분지수 가 적고 꼬투리가 달리는 포기주변 반경이 작아 기계화 재배 에 유리하며 조숙형이다. 협당립수는 2립이고 종피색은 살색 이며 100립중이 ‘대광땅콩’보다 더 무거운 91 g으로 대립종 에 속한다. ‘보원’은 그물무늬병 저항성이 ‘대광땅콩’ 보다 강 하며 수확기의 낙엽정도가 대광땅콩보다 낮고 도복저항성도 ‘대광땅콩’ 보다 더 높다. ‘보원’의 수량성은 지역적응시험에 서 3개년간 평균수량이 3,720 kg/ha으로 ‘대광땅콩’ 보다 12 ～18% 증수를 보여 전국평균 17%의 증수를 보였고 연차간 및 지역간 변이가 ‘대광땅콩’ 보다 적어 수량안정성이 높다.

Korean peanut yield by new varieties has marvelously increased since first variety with 1.09 MT/ha was developed in 1960. This means 77.58kg/ha yield increment every year from 1960 to 2012. A new peanut variety “Sinpalkwang”(Arachis hypogaea ssp. hypogaea L.) showed the highest grain yield, 5.40 MT/ha, of Korean varieties was developed at the Department of Functional Crop, NICS, in Milyang in 2012. This was developed from the cross between cultivar “Palkwang“ and crossing line from cultivar “Palkwang” and “PI156649”. “Sinpalkwang” which is Virginia plant type has 41cm of main stem length and 25 branch number per plant. 45 pod number per plant, 79% of shelling ratio and 92g of 100-grain weight in the regional yield trials(RYT) greatly contributed to increase the yield potential of this variety. Each pod has two grains with brown testa and ellipse shape. Seed quality showed 45.4% of crude oil and 29.2% of protein content. This variety also showed more resistant to web blotch and stem rot, compared with check variety, Daekwang. In the regional yield trials “Sinpalkwang” outyielded check variety by 28% with 5.40 MT/ha for kernel.

Recently, fresh peanut eaten after boil is popular to korean consumer owing to its sweet taste. A new vegetable peanut variety “Ami”(Arachis hypogaea ssp. fastigiata.) was developed at the Department of Functional Crop, NICS, in Milyang in 2012. It was developed from the cross between the short stem cultivar “Satonoka” and the large grain cultivar “Milyang 16”. “Ami” which is Shipung plant type has 50cm main stem length and 10 branch number per plant. Each pod has two grains with brown testa and long-ellipse shape and dried 100-seed weight was 84g in the regional yield trials(RYT). The Sucrose and tannin content of fresh peanut are 24.9mg/g and 4.9mg/g, respectively. Functional compound content was 3944㎍/g of Luteolin in peanut shell, This variety also showed more resistant to stem rot and lodging, compared with check variety, Palkwang. In the regional yield trials “Ami” was outyielded than check variety by 9% with 10.10MT/ha for fresh pod and by 14% with 4.51MT/ha for dried kernel.

간식용 대립 땅콩의 선호도가 높음에 따라 대립 양질 다수성 신품종 육성을 위하여 단경 박피 다수성인 ‘팔광’과 직립다수성 ‘유데라카’를 인공교배하여 ‘일평’을 육성하였다. ‘일평’은 버지니아 초형으로 개화기가 빠르고 지상부 생장습성은 단경 다분지성이다. ‘일평’은 ‘대광땅콩’에 비해 성숙협수,협실비율, 100협립수 등 양호한 등숙특성을 보이며 100립중이 85 g인 대립품종이다. ‘일평’의 도복은 매우 강한 특성을나타내며 흑반병, 그물무늬병에 비교적 강하고 낙엽율도 낮아 생육후기까지 녹엽 유지에 유리하였다. ‘일평’은 단백질이27.6%, 기름함량이 49.5%이고 지방산은 올레산과 리놀산이비슷한 비율로 나타났다. ‘일평’을 3년간 지역적응시험 결과‘대광땅콩’에 비하여 전국평균 4.47 ton로서 8% 증수되었다.‘일평’은 경장이 짧고 병해에도 강한 편이나 충분한 성숙을위하여 강원도와 산간 고냉지를 제외한 지역에서 적응성이높은 품종이다.

새로운 참깨품종 ‘밀성’을 농촌진흥청 작물시험장 영남 시험장에서 2007년에 개발하였다. 교배조합은 이리4호와 ‘수원131호’로 고품질, 다수성, 내병성을 목적으로 하였다. ‘밀성’은 계통육종법을 이용하여 생산력검정시험, 지역적응성 검정시험을 거쳐 개발되었다. ‘밀성’은 소분지이고 3과성, 2실 4방의 꼬투리를 가지고 있다. 그리고 ‘밀성’의 개화기는 7월 1일로 표준품종인 ‘양백’보다 2일 빨랐다. 또한 성숙기는 8월20일로 ‘양백’과 같았다. ‘밀성’의 경장은 129 cm로 ‘양백’과비슷했다. 하지만 ‘밀성’은 ‘양백’보다 많은 27개의 착삭절을가지고 있어 밀삭형이다. ‘밀성’의 천립중은 2.79 g으로 ‘양백’보다 컸다. ‘밀성’의 역병저항성 및 도복저항성은 ‘양백’과비슷했고 ‘밀성’의 수량은 98 kg/10a로 ‘양백’보다 5% 증수하였다. ‘밀성’은 유지함량은 ‘양백’보다 적었으나 단백질함량은 많았다. 지방산 중 올레산의 비율은 45.8%였고 세사민함량은 2.8 mg/g, 세사몰린 함량은 3.0 mg/g이었다.

A new peanut variety “Sangan”(Arachis hypogaea ssp. fastigiata L.) showed the highest yielding of Korean varieties was developed at the Department of Functional Crop, NICS, in Milyang in 2011. This was developed from the crossing line between cultivar “Palkwang” with high quality and “local collection” with short stem. “Sangan” which is Shinpung plant type has 44cm of main stem length and 10 branch number per plant. Each pod has two grains with long ellipse shape of brown testa and yield components is composed of 40 mature pods of per plant, 96g of 100-seed weight, 79% of pod shelling ratio in the regional yield trials(RYT). Seed quality showed 48.5% of crude oil and 29.1% of protein content. This variety also showed more resistant to early leaf spot and web blotch, compared with check variety, Daekwang. In the regional yield trials “Sangan” outyielded check variety by 15% with 4.87 MT/ha for kernel.

A new vegetable peanut variety “Jaseon”(Arachis hypogaea ssp. fastigiata.) was developed at the Department of Functional Crop, NICS, in Milyang in 2011. It was developed from the cross between the Large grain cultivar “YG60” and the short stem cultivar “YG55”. “Jaseon” which is Shipung plant type has 43cm main stem length and 11 branch number per plant. Each pod has two grains with brown testa with long-ellipse shaped large kernel and dried 100-seed weight was 105g in the regional yield trials(RYT). The Sucrose and tannin content of fresh peanut are 25.0mg/g and 4.4mg/g, respectively. This variety also showed more resistant to late leaf spot, stem rot and lodging, compared with check variety, Palkwang. In the regional yield trials “Jaseon” was outyielded than check variety by 15% with 10.21MT/ha for fresh pod and by 11% with 4.37MT/ha for dried kernel.

The purpose of this study was to determine the free amino acid contents, total phenolic contents and antioxidant activity in raw Sesamum indicum seeds (cv. Kopum and cv. Mihuk) and their sprouts germinated for 7 days. Total free amino acid contents of sprouts (29.34±3.3 mg/g DW) were significantly higher than that of raw seeds (6.85±0.39 mg/g DW). All individual free amino acid, including asparagine, alanin, arginine, and leicine were significantly increased in the sesame sprout. And also germinated sprout significantly increased the total phenolic contents (2.2±0.3 mg GAE/g) that resulted in the increased DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capacity. Subsequently, two varieties of sesame and its sprouts were analyzed for their phenolic constituents using high-performance liquid chromatography (HPLC). Catechin, sinapic acid and salicylic acid were identified as the major phenolic acid presented in sesame sprout. However, the major biological constituents sesamin and sesamolin content were significantly decreased during germination.

The perilla [Perilla frutescens (L.)], which belongs to the family Lamiaceae, have been used as a not only important traditional source of oil but also used traditional herbal medicine for treating various disease including depression, anxiety, tumor, cough, bacterial and fungal infections, allergy, detoxication and some intestinal disorders in east asian countries. In this context, luteolin isolated from the P. frutescens inhibited the linoleic acid peroxidation catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC50 of 5.0 μM. To investigate the inhibitory effect of luteolin on dioxygenase enzyme, we assayed soybean lipoxygenase-1 activity with the inhibitor. Soybean lipoxygenase-1 showed time-dependent inhibition in the presence of luteolin. Increasing luteolin concentrations led to the decrease in both the initial velocity (vi) and the steady-state rate (vs) in the progress curve. Thus luteolin showed a simple reversible noncompetitive slow-binding inhibition against soybean lipoxygenase-1 with kinetic parameter (k3 = 0.056 μM-1min-1, k4,= 0.006 μM-1min-1, Ki app = 0.106 μM).

The influenza neuraminidase (E.C. 3.2.1.18) is an antiviral target of high pharmaceutical interest because of its essential role in cleaving sialic acid residues from surface glycoproteins and facilitating release of virions from infected cell. In this context, polyphenolic compounds including luteolin, rosmarinic acid, and apigenin from Perilla frutescens were evaluated for their inhibitory effect on recombinant virus H1N1 neuraminidase. Among the test compounds, luteolin and rosmarinic acid inhibited the rvH1N1 neuraminidase with an IC50 of 8.4 and 46.7 μM, respectively. The inhibition kinetics analyzed by Dixon plots indicates that luteolin and rosmarinic acid are noncompetitive inhibitor and inhibition constant, Ki, were established as 14.3 and 43.9 μM, respectively. Subsequently, we also analyzed the rosmarinic acid and luteolin contents of 383 accessions of perilla seed germplasms by high performance liquid chromatography (HPLC). The rosmarinic acid and luteolin contents of perilla seeds were ranged from 15.7 μg/g to 2,894.9 μg/g and from 1.6 μg/g to 949.1 μg/g, respectively.

A total of 162 accessions of Perilla germplasm collected from diverse habitats in South Korea. The oil content of the seeds varied from 19.8 to 49.6%, the average being 33.3%. Oil content showed wide ranges of variation. The fatty acid composition of the seed oil showed the following overall ranges: palmitic 4.5 to 8.1%, stearic 1.6 to 7.4%, oleic 13.4 to 23.6%, linoleic 9.5 to 19.5%, and linolenic acid 48.6 to 66.4%. No correlation was found between oil content and fatty acid composition. The results obtained in this study provide useful background information for developing new cultivars with a high oil content and different fatty acid. Several accessions could be used as parental lines in breeding programs aiming to increase perilla oil quantity and quality.

This experiment was conducted to select cultivar suitable for peanut sprout from general Korean peanut cultivars developed already. On sprouting peanut, it rapidly increase the content of trans-resveratrol that has effects on anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular in mouse. For this experiment we tested peanut sprout characteristics on 37 cultivars cultivated for 7 days at 26℃ temperature. The characteristics among cultivars showed the variations as follow: The range of 100 grain weight is 56 to 142, hypocotyl length 4.3 cm to 5.8 cm, diameter of hypocotyl 5.0 to 8.0 mm, epicotyl 0.8 cm to 4.6 cm, seedling ratio per seed number 84 % to 100 %, weight per seedling 4.9 g to 8.4 g, the rate of hypocotyl cleavage 0 % to 46 %, the content of trans-resveratrol 22.5 ㎍/g to 88.2 ㎍/g and sprout yield 360 % to 820%. We considered the selecting points as high sprout yield, high seedling rate, high resveratrol content, high low brownish cotyledon, no hypocotyl cleavage, and fat hypocotyl etc. Fortunately, the best cultivar "Jokwang" was selected on these conditions with 7.8 mm diameter, clean cotyledon color, 100 % seedling rate, 0 % hypocotyl cleavage, 63.3 ㎍/g resveratrol, and 820 % sprouting yield. This cultivar may use as new food and nutraceutical material.

This study was conducted to evaluate the growth characters and yield components of 18 collected sesame cultivars to get basic information on the variation for the sesame breeding using principal component analysis. All characters except days to flowering, days to maturity and 1,000 seed weight showed significantly different. Seed weight per 10 are showed higher coefficient of variance. Capsule bearing stem length and liter weight showed positive correlation with seed yield per 10 are. The principal components analysis grouped the estimated sesame cultivars into four main components which accounted for 83.7% of the total variation at the eigenvalue and its contribution to total variation obtained from principal component analysis. The first principal component (Z1 ) was applicable to increase plant height, capsule bearing stem length and 1,000-seed weight. The second principal component (Z2 ) negatively correlated with days to flowering and maturity by which it was applicable to shorten flowering and maturity date of sesame. At the scatter diagram, Yangbaek, Ansan, M1, M2, M4, M7 and M9 were classified as same group, but M10, Yanghuk, Kanghuk, M5, M6, M12 and M13 were classified as different group. This results would be helpful for sesame breeder to understand genetic relationship of some agronomic characters and select promising cross lines for the development of new sesame variety.

This study was conducted to improve the quantity of fatty acid in peanut oil for quality breeding program. To determine fatty acids of oleic acid and linoleic acid in F2 generation, we used near infrared reflectance spectroscopy (NIRS) with high R2 values of 0.983 and 0.991 for oleic and linoleic acids, respectively. For this experiment, two combinations with 2 mother plant( cultivar Sangpyeong and Baekseon) and 1 father plant (YGC 2) were analysed. Each cultivar Sangpyeong, Baekseon, and YGC-2 in oleic and linoleic acid have 41.6%, 58.8%, 76.9% and 44.9%, 26.2%, 5.7% respectively. Distribution of fatty acid in combination Sangpyeon/YGC-2 in oleic acid showed 38.2% to 83.5%, and in linoleic acid showed 43.2% to 6.3%. The combination of Baekseon/YGC2-2 also showed 44.6% to 81.0% in oleic acid, and 40.1% to 6.8%. This result showed the values of fatty acid in F2 generation had more wide continuous variation, compared with parents values.

To obtain some information on the change of antioxidant components of seeds during grain filling stage as affected by the sowing dates, lignan compounds were investigated according to days of flowering under different sowing dates. Sesamin and sesamolin contents showed significantly different by days of flowering and varieties. Both of sesamin and sesamolin content increased after flowering and reached highest at 40 days of flowering, but they started to decrease thereafter. Sesamin and sesamolin contents of sesame seeds changed with sowing dates. Generally, late sowing date of May 30 showed relatively higher accumulation rate of sesamin and sesamolin contents rather than other sowing dates, but overall patterns were a little different by varieties and lignan compounds. In case of variety Yangbaek, sesamin and sesamolin contents showed relatively higher at sowing date of May 30, but variety Yanghuck showed higher sesamin and sesamolin contents till 20 days of flowering when sowing date of May 30, but it showed to change that both lignan contents were relatively higher under sowing date of May 10.

Application of genotype by environment (G~;~times~;E ) interaction would be used for identifying optimum test condition of the varietal adaptation in the establishment of breeding purpose. Yield and yield components were used to perform additive main effect and multiplicative interaction (AMMI) analysis. Significant difference for G~;~times~;E interaction were observed for all variable examined. For yield, 0.18 of total sum of squares corresponded to G~;~times~;E interaction. Correlation analysis was carried out between genotypic scores of the first interaction principal component axis (IPCA 1) for agronomic characters. Significant correlations were observed between IPCA 1 for yield and capsule bearing stem length (CBSL), number of capsule per plant (NOC). The biplot of grain yield means for IPCA1 which accounted for 34% of the variation in total treatment sums of squares showed different reaction according to G~;~times~;E interaction, genotypes and environments. Taegu showed relatively lower positive IPCA1 scores, and it also showed smaller coefficient variation of yield mean where it is recommendable as a optimal site for the sesame cultivar adaptation and evaluation trial. In case of variables, Yangbaek and M1 showed relatively lower IPCA1 scores, but the score direction showed opposite each other on the graph. Ansan, Miryang1, Miryang4, and Miryang6 seemed to be similar group in view of yield response against IPCA1 scores. These results will be helpful to select experimental site for sesame in Korea to minimize G~;~times~;E interaction for the selection of promising genotype with higher stability.