기후변화, 외래병해충발생, 밀원수 감소 등 여러 요인으로 인해 우리 나라의 양봉농가는 벌꿀생산량 및 농가소득이 감소하는 추세이다. 또한 밀원이 없는 시기에 가축용 사료가 아닌 고가의 식용설탕을 꿀벌의 인공사료로 급여하고 있어 농가는 ‘생산비 상승’과 ‘밀원수 부족’을 국내양봉업이 직면한 가장 큰 문제점으로 지적하고 있다. 이에 따른 양봉농가의 생산비를 절감하고 꿀벌의 생리·생태의 개선효과를 가지는 저가형 당액사료개발을 위해 농산부산물에서 추출한 천연당을 봉군에 적용시험을 추진하였다. 양파(OS-1, OS-2), 양배추(CS-1, CS-2), 바나나(Banana)부산물에서 추출한 천연당의 봉군당 평균먹이소모량(mL)은 설탕당액(대조구) 처리구와 비교시 바나나추출당액 급여구에서 5500.00±0.00, 5133.33±404.15로 유사하였다. 그러나 꿀벌의 봉세발달은 대조구에 비해 21006.7±2137, 17403.3±2257마리로 조사되어 낮은 경향을 나타내었고 수명조사 결과는 큰 차이가 없었다. 농산부산물을 이용한 천연당은 우선 꿀벌의 기호도 향상을 우선으로 개선시켜야 할 것으로 보인다.

농촌진흥청 개발 품종인 토종벌 신품종(질병저항성 우수계통)에 대해 전남지역에서의 형질특성(봉세발달, 마리당 수밀력, 청소력 등)을 조사하였다. 질병저항성 계통(RS)으로 육성된 토종벌의 유밀기 마리당 수밀력은 들어오는 일벌의 무게가 80.89±8.95mg, 나가는 일벌의 무게가 63.56±8.90mg으로 조사되었고, 비저항성계통(NRS)은 각각 83.22±1.39, 66.67±1.20mg으로 조사되어 RS에서 그 무게차가 0.7mg 더 높았으나 통계적 유의차는 없었다. Pin killed test에 의한 사충제거능력(청소력)은 제거하지 못한 유충잔존율이 RS에서 12시간과 24시간 경과시 14.00±10.39와 7.00±3.46으로, NRS에서는 20.33±14.29, 13.33±10.41%로 조사되었으며, 특히 RS와 NRS의 사충 비제거율이 24시간 경과하였을 때 4.67±2.08, 8.33±7.77%로 RS가 우수한 특성을 보였다. 신규여왕벌 입식 후 봉군세력발달은 NRS에 비하여 RS의 일벌수, 번데기 및 유충수의 발달율이 안정적이고 다소 빠르게 증가하였다.

All aspects of hosts’ life are affected not only by symbiotic bacterial community but also endosymbionts, such as physiological function, mating preference and fitness. Wolbachia, one of the most common endosymbionts, known as a manipulator of host sex ratio, may also have a huge effects on other symbionts. Here, we assessed the effects of Wolbachia on the diversity and structure of other symbiotic microbial communities in six ant species by using next-generation sequencing. Our results show that the diversity of the symbiotic microbial communities between Wolbachia-positive species and negative species is not different. However, according to the Wolbachia infection, dominant symbiotic microbes of each host species were different as well as the composition of the overall symbiotic microbial community. Thus, this study suggests that, as Wolbachia have an important role in shaping microbiome of hosts, endosymbiont infection status deserves special consideration in studying microbiome.

Haplodiploid sex determination is prevalent in nature comprising about 25% of higher taxa. Due to its uniqueness, selfish entity may relatively easily slip in the system. Here, I will introduce 3 different types of selfish entities. First, some microorganisms are known to distort sex ratio in favor of their own prosperity. Second, a genetic fragment, called paternal sex ratio chromosome, is classified as the extremely selfish element so far in haplodiploid. Finally, haploid genome itself can evolve selfishness. The first two result in skewed sex ratio, and the last in completely new genetic relatedness. I will also discuss their evolutionary significance and potential utilization in controlling pest populations.

By definition, endosymbionts reside in the hosts' cells and are maternally inheritted to offspring as the mitochondriondoes. Recent progresses reveal that some endosymbionts have evolved their manipulabilities of hosts' reproduction. Inthis nature, once an endosymbiont shares its host with others, their association may last long unless their evolutionaryinterest or hosts' fitness is undermined.We tested whether the association existed among the endosymbionts; Wolbachia, Spiroplasma, Cardinium and Rickettsia.The results show that there are some multiple infection cases but there is no obvious pattern in their association.

Xenotransplantation is proposed as a solution to the problem of organ shortage. However, transplantation of xenogeneic organs induces an antigen-antibody reaction in α-1,3-gal structure that are not present in humans and primates, and thus complement is also activated and organs die within minutes or hours. In this study, we used FasL gene, which is involved in the immune response of NK cell, and US11, which suppresses MHC Class I cell membrane surface expression, to inhibit cell mediated rejection in the interspecific immunity rejection, and also hDAF(CD55) was introduced to confirm the response to C3 complement. These genes were tranfeced into Korean native pig fetal fibroblasts using pCAGGS vector. And cytotoxicity of NK cell and human complement was confirmed in each cell line. The US11 inhibited the cytotoxicity of NK cell and, in addition, the simultaneous expression of US11 and Fas ligand showed excellent suppress to T-lymphocyte cytotoxicity, hDAF showed weak resistance to cytotoxicity of natural killer cell but not in CD8+ CTLs. Cytotoxicity study with human complement showed that hDAF was effective for reducing complement reaction. In this studies have demonstrated that each gene is effective in reducing immune rejection.

For the development of processed food for hypoglcemia, it is important to construct model system to confirm factors that reduce the glycemic index in real food. This study was to investigate the relationship between the gel model system and real food the high amylose type of segoami using response surface methodology. The independent variables were concentration (X1; 10, 15, 20, 25, and 30%) and steaming time (X2; 30, 35, 40, 45, and 50 min). The predicted glycemic index (Y1) was analyzed as a dependent variable. The regression of pGI was 0.7343, indicating that the model fits the data well in the prediction test. The predict glycemic index of the gel was in the range of 71.38 ~ 83.78, depending on the gel preparation conditions and predicted optimum condition was 23.7% gel concentration and 43.8 minutes of gelatinization. The predict glycemic index of rice gel were decreased with an increase of gel concentration, rather than the steaming time. In order to confirm the effect on in vitro digestibility in real food, the real food (Garraedduk) was prepared by applying the optimal conditions. As a result, there was no significant difference between the predicted value (77.1) and the experiment value (76.6). This result showed that the in vitro digestibility in real food can be predicted by applying the gel model system.

Pt@Cu/C core-shell catalysts were successfully prepared by impregnation of a carbon support with copper precursor, followed by transmetallation between platinum and copper. The Pt@Cu/C core-shell catalysts retained a core of copper with a platinum surface. The prepared catalysts were used for hydrogen production through catalytic dehydrogenation of decalin for eventual application to an onboard hydrogen supply system. Pt@Cu/C core-shell catalysts were more efficient at producing hydrogen via decalin dehydrogenation than Pt/C catalysts containing the same amount of platinum. Supported coreshell catalysts utilized platinum highly efficiently, and accordingly, are lower-cost than existing platinum catalysts. The combination of impregnation and transmetallation is a promising approach for preparation of Pt@Cu/C core-shell catalysts.

To improve its textural properties as a support for platinum catalyst, carbon aerogel was chemically activated with KOH as a chemical agent. Carbon-supported platinum catalyst was subsequently prepared using the prepared carbon supports(carbon aerogel(CA), activated carbon aerogel(ACA), and commercial activated carbon(AC)) by an incipient wetness impregnation. The prepared carbon-supported platinum catalysts were applied to decalin dehydrogenation for hydrogen production. Both initial hydrogen evolution rate and total hydrogen evolution amount were increased in the order of Pt/CA < Pt/AC < Pt/ACA. This means that the chemical activation process served to improve the catalytic activity of carbon-supported platinum catalyst in this reaction. The high surface area and the well-developed mesoporous structure of activated carbon aerogel obtained from the activation process facilitated the high dispersion of platinum in the Pt/ACA catalyst. Therefore, it is concluded that the enhanced catalytic activity of Pt/ACA catalyst in decalin dehydrogenation was due to the high platinum surface area that originated from the high dispersion of platinum.

We used the three kinds of mosquito traps (Black-hole with UV light, CO2-baited Mos-hole with the newly developed attracting-solvent, CO2-baited DMS; Digital Mosquito Monitoring System) to know their collecting efficiency for the female mosquitoes in Korean rural areas. The Black-hole mosquito trap caught many kinds of insects including only few female mosquitoes. The Black-hole trap has the UV-light and the light seemed to attract other terrestrial and aquatic insects, such as the common flies, May flies, and the stone flies. Even though the trap was developed to collect mosquitoes, the trap caught only few of female mosquitoes less than 1% of all insects caught. Their selective efficiency to collect the female mosquitoes was relatively lower than other two kinds of traps. The Mos-hole and CO2-baited DMS traps had the collecting efficiency of over 80% to collect the female mosquitoes. The two traps caught the relatively lower number (less than 3% of total insects) of other insects, such as few Coleoptera and Diptera, and their collecting efficiency for the female mosquitoes was very higher. Generally speaking, mosquitoes disliked the UV light but they relatively preferred CO2 gas including the attracting-solvent. They had also been attracted the acidic solvent with CO2 gas. If we could use the efficient and selective mosquito traps with the fully understanding about the mosquito habits, we could assume that we can keep the biodiversity high around the mosquito habitats as well as to save money for the insect pest control.

This snag-dwelling arthropod community study was conducted for the ecological evaluation of dead woods at Korean fir stand in Mt. Woonak in Pocheon-si, Gyeonggi-do, from April 2010 to August 2011. We put a windows trap and an emergence trap on the trunk of each snag, and we selected the six snags during the study periods. We collected 3930 individuals (5 class, 21order, 52 families) but we didn’t include the number of unidentified larva. We separated those individuals into the three functional groups and we found out the proportion and number of each functional group from the total individuals: herbivores (27.6%, 1083) predators (10.9%, 430), detritivores (61.4%, 2413), etc(0.1%, 4). We found out the proportion and number of each taxon group for herbivores, Armadillidae (0.15%, 6), Acarina (2.57%, 101), Psocoptera (0.25%, 10), Hemiptera (0.46%, 18), Mecoptera (0.05%, 2), Hymenoptera (not ant) (5.14%, 202), Aphididae (3.82%, 150), Cicadellidae (0.4%, 16), Curculionidae (3.61%, 142), Chrysomelidae (0.23%, 9), Elateridae (3.36%, 132), Erotylidae (3.16%, 124), Nitidulidae (2.6%, 102), Pyrochoroidae (0.08%, 3), Scarabaeidae (0.31%, 12), and Cetoniidae (0.13%, 5). Predators were consisted of the following taxa groups: Araneae (2.9%,114), Chilopoda (0.31%, 12), Formicidae (4.25%, 167), Carabidae (0.08%, 3), Staphylinidae (1.09%, 43), Cleridae (0.05%, 2), Pselaphidae (0.1%, 4), Colydiidae (0.38%, 15), Harpalidae (0.1%, 4), Histeridae (0.36%, 14), and Dermestidae (0.8%, 31). Detritivores were consisted of the following taxa groups: Millipedes (0.92%, 36), Archaeognatha(1.6%, 63), Diptera (7.81%, 307), Collembola (35.47%, 1394), Protura (0.03%, 1), Dermaptera (0.1%, 4), Tettigoniidae (0.08%, 4), Raphiidophoridae (0.03%, 1), Ipidae (14.12%, 555), Silphidae (0.15%, 6), Cuculidae (0.15%, 6), Cerambycidae (0.38%, 15), Oedeeridae (0.03%, 1), Lucamnidae (0.03%, 1), Stenotrachelidae (0.05%, 2), Buprestidae (0.13%, 5), Tenebrionidae (0.23%, 9), and Mordellidae (0.1%, 4), etc. Conclusively, the snag plays an important roll as the diverse arthropods’ habitats in the Korean fir forest ecosystem.

The purpose of this study was to comparatively analyze the active stability of shoulder joints according to the frequency of overhead motions, such as serving and spiking, engaged in by female professional volleyball athletes who play different positions, and to provide the results as the basic data for developing exercise programs to prevent shoulder joint injuries. The subjects of this study were 50 Korean female professional volleyball players and positions were as follows: left and right attackers, centers, setters, and liberos. The external rotation and internal rotation muscle strength and muscle strength ratios of the dominant and non-dominant arms of all subjects were measured using Biodex. The results of this study are as follows: Frist, no significant differences were found in the internal and external rotation muscle strength of the dominant and non-dominant arms between positions. Second, for the shoulder joint muscle strength ratio of the dominant arm, by position, the setter showed significantly greater stability compared to the other positions. Third, for the shoulder joint muscle strength ratio of the non-dominant arm, by position, no significant difference in stability between positions was found. Fourth, it was found that the dominant arm had significantly greater instability of the shoulder joint than the non-dominant arm for attackers and centers, but no significant difference was found for setters and liberos. This study comparatively analyzed the muscle strength ratios of the external/internal rotations and dominant/non-dominant arms, which can determine the stability of the shoulder joints between female professional volleyball playing positions that engage in jumps and spikes using only the dominant hand and positions that do not.

Bee venom contains a variety of peptides and enzymes, including serine proteases. While the presence of serine proteases in bee venom has been demonstrated, the role of these proteins in bee venom has not been elucidated. Furthermore, there is currently no information available regarding the melanization response or the fibrin(ogen)olytic activity of bee venom serine protease, and the molecular mechanism of its action remains unknown. Here we show that bee venom serine protease (Bi-VSP) is a multifunctional enzyme. In insects, Bi-VSP acts as an arthropod prophenoloxidase (proPO)-activating factor (PPAF), thereby triggering the phenoloxidase (PO) cascade. Bi-VSP injected through the stinger induces a lethal melanization response in target insects by modulating the innate immune response. In mammals, Bi-VSP acts similarly to snake venom serine protease, which exhibits fibrin(ogen)olytic activity. Bi-VSP activates prothrombin and directly degrades fibrinogen into fibrin degradation products, defining roles forBi-VSP as a prothrombin activator, a thrombin-like protease, and a plasmin-like protease. These findings provide a novel view of the mechanism of bee venom in which the bee venom serine protease kills target insects via a melanization strategy and exhibits fibrin(ogen)olytic activity.

This study was estimated for cabbage aphid, Brevicoryne brassicae in Brassica campestris L. var. rapa (L.) Hartm. in order to institute of Economic injury levels(EILs). B. brassicae was innoculated on April 29, in differently 0, 5, 10, 20 and 40 adults per ten plant, respectively. After inoculated of B. brassicae, the density was increased until harvest ing gradually in all plots except non innoculate plot. and Higher inoculation density were increased higher than lower inoculation density. Percentage of damage leaf was higher in plots with higher initial aphid density than in plots with lower initial aphid density. And the leaf weight of commodity were decreased in higher initial aphid density. The decreasing rates of leaf weight of commodity was increased with increasing initial aphid density. The relationship between initial B. brassicae densities and the decreasing rates of leaf weight of commodity was well described by a linear regression, Y=0.8416X-3.5147, R2=0.94. Based on the relationship, the number of adults per 10 plant which can cause 5% loss of yield was estimated to be approximately 10.1. And EILs was estimated to be approximately 1.0 adults/plant in late April.