본 연구에서는 오미자 추출물이 보유하고 있는 것으로 알려진 항산화, 항균, 항염증 활성의 용매별 추출에 따른 변화를 구명하고 관련 기초자료를 제공하고자 하였다. 오미자를 물과 70% 에탄올, 99.9% 메탄올로 추출한 후, 폴리페놀 함량, 라디칼 소거능, SOD 유사활성, 항균활성, NO 생성 저해능을 평가하였다. 그 결과, 오미자 물 추출물의 폴리페놀 함량은 9.2 mg/g이었고 에탄올과 메탄올 추출에 의해서 각각 70.7%, 77.2% 증가하였다. DPPH 라디칼 소거능은 에탄올 추출물에서 IC50이 399.7 μg/mL으로 항산화능이 가장 높았고, 메탄올 추출물(400.8 μg/mL), 물 추출물(992.4 μg/mL)의 순으로 나타났다. 이를 통해, 물 추출물보다 유기용매 추출물의 폴리페놀 함량이 높음에 따라 소거활성이 증가하여, 폴리페놀 함량과 라디칼 소거활성은 연관성이 있음을 확인하였다. 병원균 3종 E.coli, S. typhimurium, St. aureus에 대한 항균 활성은 물 추출물의 경우 38.9~64.5%인 반면 유기용매 추출물에서는 70.0 ~ 85.6%로 증가하였다. 반면에 NO 생성에 대해서는 물 추출물이 대조군에 비해 35.7% 저해시켰으나 에탄올과 메탄올 추출에 의해서 각각 22.1%, 25.7% 감소하였다. 결론적으로 오미자의 유기용매(에탄올, 메탄올) 추출물은 물 추출물에 비하여 라디칼 소거능과 SOD 유사 활성, 항균 활성이 우수하였다. 반면에 항염증능은 유기용매 추출물에 비해 물 추출물이 우수한 활성을 보였다. 본 실험결과는 오미자 추출물을 건강 기능식품 개발 소재나 가공식품으로 활용할 때 주요 대상으로하는 건강기능성을 증진시키는데 기초자료로 활용할 수 있을 것으로 생각된다.

Despite many researches related with in-vitro culture of porcine spematogonial stem cells (SSCs), adherent culture system widely used has shown a limitation in the maintenance of porcine SSC self-renewal. Therefore, in order to overcome this obstacle, suspension culture, which is known to have numerous advantage over adherent culture, was applied to the culture of porcine SSCs. Porcine SSCs retrieved from neonatal testes were suspension-cultured for 5 days or 20 days, and characteristics of suspension-cultured porcine SSCs including proliferation, alkaline phosphatase (AP) activity, and self-renewal-specific gene expression were investigated and compared with those of adherent-cul-tured porcine SSCs. As the results, the suspension-cultured porcine SSCs showed entirely non-proliferative and significantly higher rate of AP-positive cells and expression of self-renewal-specific genes than the adherent-cultured porcine SSCs. In addition, long-term culture of porcine SSCs in suspension condition induced significant decrease in the yield of AP staining-positive cells on post-day 10 of culture. These results showed that suspension culture was inappropriate to culture porcine SSCs, because the culture of porcine SSCs in suspension condition didn’t stimulate proliferation and maintain AP activity of porcine SSCs, regardless of culture periods.

본 연구는 짚신나물 열수 추출물의 α-glucosidase 저해 활 성을 측정하고, 분화된 근육세포에서 glucose 이용과 인슐린 신호전달에 미치는 영향을 분석하였다. 짚신나물 열수 추출 물(10 ㎎/㎖)은 α-glucosidase 활성을 67% 저해하였으며, 같은 농도의 양성대조구인 acarbose(63%)와 유사한 저해 효과를 보였다. 짚신나물 열수 추출물이 α-glucosidase에 의한 단당 류 생성을 저해함으로 식사 후 혈당이 급격히 상승하는 것을 억제하는데 효과적인 소재로 이용 가능성을 확인하였다. 또한 근육세포에서 인슐린 저항성을 유발하기 위해 지방산(1 mM, palmitic acid)를 처리하였고, glucose의 세포내 유입이 감소 되는 것을 확인하였다. 지방산 처리 세포 모델에서 짚신나 물 열수 추출물(10 ㎍/㎖)은 glucose 이용을 유의적으로 회복 시켜 주었다. Normal 상태의 배양조건에서 근육세포의 포도 당 이용능은 짚신나물 열수 추출물(100 ㎍/㎖) 처리에 의해 유의적으로 증가하였다. 근육세포 내로 glucose 유입은 운반 단백질인 Glut4를 통해 이루어지며, 이것은 인슐린이 신호전 달을 통해 조절한다. 짚신나물 열수 추출물의 세포 내 glucose 이용 증가 효과는 인슐린 신호전달 관련 분자인 Akt 유전자 와 단백질 발현을 증가시킨 것과 관련되는 것으로 추정된다. 결론적으로, 짚신나물 열수 추출물은 소화기관에서의 탄수화 물 흡수 저해와 근육세포 내 glucose 이용 증가를 통해 혈당 조절 및 당 대사 개선에 긍정적인 영향을 미치고 있음을 확인 하였다.

Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an im-portant source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differ-entiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphoryla- tion, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.

Satellite cells were derived from muscular tissue in postnatal pig. Satellite cell is an important to growth and development in animal tissues or organs. However, the progress underlying induced differentiation is not clear. The aim of this study was to evaluate the morphologic and the transcriptome changes in porcine satellite cell (PSC) treated with insulin, rosiglitazone, or dexamethasone respectively. PSC was obtained from postnatal muscle tissue. In study 1, for study the effect of insulin and FBS on the differentiated satellite cells, cells were cultured at absence or presence of insulin treated with FBS. Total RNA was extracted for determining the expression levels of myo-genic PAX3, PAX7, Myf5, MyoD, and myogenin genes by real-time PCR. Myogenic genes decreased expression levels of mRNA in treated with insulin. In study 2, in order to clarify the relationship between rosiglitazone and lipid in differentiated satellite cells, we further examined the effect of FBS on lipid accumulation in the presence or absence of the rosiglitazone and lipid. Significant differences were observed between rosiglitazone and lipid by FBS. The mRNA of FABP4 and PPARγ increased in rosiglitazone treatment. In study 3, we examined the effect of dexame-thasone on osteogenic differentiation in PSC. The mRNA was increased osteoblasotgenic ALP and ON genes treated with dexamethasone in 2% FBS. Dexamethasone induces osteoblastogenesis in differentiated PSC. Taken together, in differentiated PSCs, FABP4 and PPARγ increased to rosiglitazone. Whereas, no differences to FBS and lipid. These results were not comparable with previous reports. Our results suggest that adipogenic, myogenic, and osteoblasto-genic could be isolated from porcine skeletal muscle, and identify culture conditions which optimize proliferation and differentiation formation of PSC.

Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90∼100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson’s, oil red O, and Alizarin red staining respectively. We per-formed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator- acti-vated receptor gamma (PPARγ) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteo-blast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were indu-ced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strat-egies for augmenting meat quality.

The centipede Scolopendra subspinipes mutilans has been a medically important arthropod species by using it as a traditional medicine for the treatment of various diseases. In this study, we derived a novel lactoferricin B like peptide (LBLP) from the whole bodies of adult centipedes, S. s. mutilans, and investigated the antifungal effect of LBLP. LBLP exerted an antifungal and fungicidal activity without hemolysis. To investigate the antifungal mechanism of LBLP, a membrane study with propidium iodide was first conducted against Candida albicans. The result showed that LBLP caused fungal membrane permeabilization. The assays of the three dimensional flow cytometric contour plot and membrane potential further showed cell shrinkage and membrane depolarization by the membrane damage. Finally, we confirmed the membrane-active mechanism of LBLP by synthesizing model membranes, calcein and FITC-dextran loaded large unilamellar vesicles. These results showed that the antifungal effect of LBLP on membrane was due to the formation of pores with radii between 0.74 nm and 1.4 nm. In conclusion, this study suggests that LBLP exerts a potent antifungal activity by pore formation in the membrane, eventually leading to fungal cell death.

보상만족도는 직무성과, 직무몰입, 그리고 근속 등과 밀접한 관계가 있는 것으로 알려져 있다. 과학기술인력의 보상만족도도 이와 유사하며, 많은 선행 연구들은 현재의 과학기술 경쟁력 저하 및 인력 수급의 애로와 관련된 많은 문제에 대해 과학기술인력에 대한 왜곡된 보상체계를 근본적인 원인으로 꼽고 있다. 이에 본 연구는 산학연 연구기관에 현재 근무하고 있는 과학기술분야 연구 인력을 대상으로 과학기술인력들이 인지하는 보상만족도의 결정요인과 이들의 영향 정도에 대하여 실증적으로 연구하였다. 또한 이러한 보상만족도가 실제로 근속과 어떠한 영향 관계를 미치는지에 대해서도 살펴보았다. 연구 결과, 과학기술인력의 보상만족도는 직무성취도, 경제적 보상, 사회적 보상의 순서로 영향을 미치는 것으로 나타났다. 연구 인프라를 포함한 연구 환경은 보상만족도에 유의미한 영향이 나타나지 않았다. 다시 말해 과학기술인력들은 생활인적 속성을 가지고 있는 동시에, 고도화된 지식전문인으로서 지적호기심과 지적탐구욕의 충족과 같은 비경제적 보상에 민감한 지식인적 속성을 함께 가지고 있었다. 따라서 과학기술인력 보상체계 개선을 위해서는 첫째, 연구자율성 확대와 연구결과에 입각한 평가 등을 보완하여 직무성취도 향상을 도모해야 할 것이고 둘째, 상대적 열위에 있는 과학기술인력의 경제적 보상을 확대하여야 할 것이며 셋째, 인력유동성과 직업안정성 향상 및 사회적 지위 개선과 같은 사회적 보상을 확대하여야 할 것이다.

In the field of fashion in this modern era, the tendency of diversification has created a variety of hybrid patterns and this tendency is being accelerated simultaneously by the hybrid trend. This research aims to analyze the aesthetic features of hybrid fashion in the modern "Sports Inspired Fashion(SIF)." We analyze fashion in the past 10 years from 2001 S/S to 2010 S/S. We focus on the four largest worldwide collections from New York, Paris, Milano, and London and concentrate on analyzing the contents from 38 volumes of Gap Press magazine. To accomplish our goal of study, we first define the concept of sports-inspired fashion and propose a framework of analysis to study hybrid patterns by reviewing the previous studies on hybrid patterns. Second, we analyze a wide range of sports-inspired fashion examples that have appeared in Gap Press magazines for the past 10 years(those which have been inspired by sports uniforms and training wears). Third, we analyze and classify the hybrid patterns of sports-inspired fashion. The results of our research are as follows. We have collected a total of 534 SIF works from the whole set of 61359 pictures and examined 23 sports fields. In terms of seasonal changes, the SIF works were found the most in the spring collections. Then, we have identified 25 hybrid patterns. The time hybrid pattern comprises more than half of the data(58.2%). The class-culture hybrid patterns comprise 18.8%, while the gender hybrid patterns comprise 18.2%. However, the intercultural hybrid patterns were rarely found, comprising merely 5% of the data. Our analysis confirms that sports and sports wear fashion are changing and developing in truly diverse ways in this modern era. This trend has continued to influence the high fashion in the modern age and is expected to exert a continuous impact on the change of fashion in the future.

This research is a study of fashion design that applied formative features of formal beauty of architecture into clothing design; we focused on Gaudi's architectural style as well as Art Nouveau style that became popular from the end of the 19th century to the beginning of the 20th century. We noted that in general the simple and flat features of cloth impose a limitation on the expression of formal features in clothing design, but a unique diversity of designs can be achieved, evoking a sense of freshness by an ideal combination of flat patterns and draping. The aim of this research is to present a possibility of extending the sphere of design expression by creating threedimensional clothes with pattern-cutting skills and applications of three-dimensional patterns as well as flat patterns found in Gaudi's works of architecture that are distinguished in curvaceousness and formal beauty. As for the research method, we reviewed previous studies by making a close review of books, papers, the pictures and web sites related to this topic. We made our clothes on the basis of this theoretical consideration. We found the following points. First, by presenting a work of fashion inspired by architectural designs, we realized that formal beauty in architect can become a motive for clothing design in a broad scale by noting the formal images, decoration details, and formative features of architectural works. Second, the characteristic lines of Gaudi's architecture are suitable to be adapted for expressing the detailed lines of decoration in clothes. Third, we can express formative beauty in clothes by highlighting the variation of shapes and lines through various attempts of change in background pattern, even though there is a limitation in the availability of cloth material because we must choose pieces of cloth with right texture and thickness that can be cut and sewn appropriately to express formative beauty. Fourth, we confirmed that it was possible to create unique formative designs by a creative application of both flat and three-dimensional cutting.