It is necessary to understand of temporal and spatial dynamics by establishing a periodical monitoring system for theproper management in small brown planthopper (SBPH). A dataset is including the number of SBPHs by location, collectionmethod [aerial collection net (AeCN) or light trap (LT)] and period (May~Aug.) for five years (2011~2015), and missingvalues were imputed using multiple imputation methods. Of the 15,848 individuals collected, approximately 47% and 52.9%were collected using the AeCN and LT methods, respectively. A high incidence of migratory SBPHs was observed duringJulian days 144-166 using the AeCN method. Generally, the migratory SBPHs from China composed 39.4% of the totalpopulations of SBPHs. These results would provide valuable information to predict the incidence period of migratory SBPHsand establish a proactive management system against SBPH.

Prediction of the seasonal occurrence and potential distribution of agricultural pests has accomplished by software toolsimplementing species distribution models (SDMs). In this aspect, we used CLIMEX software to evaluate the seasonaloccurrence and potential distribution of Indian meal moth, Plodia interpunctella (Hübner), which is one of household mothsdamaging dried fruits in pantries. Based on the simulation, the beginning of period for suitable climate was predictedto be from mid-March to end-March, while it might be end in late October to early November. The peak time for P.interpunctella was ranged from early or mid-July to mid-August, but depended on local geography. When applying RCP8.5 climate change scenario, it was predicted that P. interpunctella would not occur due to intensive rainfall in July andAugust in 2060.

The diamondback moth, Plutella xylostella is one of the world’s major pests. Economic cost to control this pest wasestimated between US$1.3 billion and US$2.3 billion based on management costs. Conservative estimate included yieldloss caused by 5% diamondback moth was estimated US$4 billion-US$5 billion. P. xylostella was managed by chemicalinsecticide such as organophosphates, carbamates and pyrethroids. But insecticide resistance which is caused by repeatedapplication makes it difficult to control this pest. For environmental friendly control of diamondback moth, entomopathogenicfungi could be used as alternative. We conducted bioassay to select high virulent isolate to larva of diamondback mothwith forty six entomopathogenic fungi which were isolated from soil samples by insect-bait method. As a result of bioassaytwelve isolates was selected as candidate. We investigated control efficacy of these twelve isolates with potted Chinesecabbage at laboratory and greenhouse.

Entomopathogenic fungi have been used to control pest as alternative to chemical pesticide. To kill the pest entomopathogenicfungi penetrate cuticle of pest, reach the hemocoel and utilize nutrient of host pest. Finally fungi kill the host by consumingthe host nutrient and physically damaging the tissues. But these process of fungi to control pest is needed so much timeand this point is a disadvantage for fungi. Therefore we studied other application method of fungi to control pest. Weconducted behavior test of beet armyworm to Isaria fumosoroseus which is high virulent against beet armyworm. Adultof the beet armyworm avoided oviposition at Chinese cabbage treated with I. fumosoroseus compare to control and otherhigh pathogenic isolate, Metarhizium anisopliae and this repellency of I. fumosoroseus lasted for 5days in greenhouse.Behavior of larvae to I. fumosoroseus also investigated with choice and non-choice test. Third to fifth instar larvae detectedand avoided fungi. Repellent behavior of larvae to fungi was more noticeable in younger larvae. This result may be usedto prevent the infestation of moth in crop production.

The sweetpotato whitefly Bemisia tabaci is one of the most serious pests in the greenhouse. They can infest variouscrop plants even in high warm greenhouse condition in summer. To understand the effect of heat acclimation ability ofB. tabaci, mortality was determined at various temperature conditions of the greenhouse. Adult whiteflies survived evenin 63°C in tomato greenhouse. The median lethal temperature (LT50) of the greenhouse colony was 59.9°C however LT50of the lab colony was 47.6°C. The relative expression level of heat shock proteins (hsp23, hsp70 and hsp90) were significantlylower in the greenhouse colony compare to the lab colony. This result suggests B. tabaci has very high thermal adaptability(acclimation) and its mechanism is associated with heat shock protein system.

Aphidius colemani has been used as commercially available natural enemies for the aphid control. However, some siblingspecies are difficult to distinguish their morphology even though they have different parasitic characteristics. Recentlywe identified overwintering parasitoids inside the aphid mummies at the pepper greenhouse in Yecheon. The nucleotidesequence analysis identified as A. transcaspicus which is morphologically similar with A. colemani. Those genetic differencesof COI and ITS2 sequences were 4.36-4.84% and 2.65-5.84%, respectively. While A. colemani is useful for the controlof Aphis gossypii and Myzus persicae, A. transcaspicus parasitize various species belongs to the genera Hyalopterus, Melanaphisand Rhopalosiphum. Thus, precise diagnosis of natural enemies is required for the successful pest control.

West Nile Virus (WNV) is transmitted by infected mosquitoes. Vector mosquitoes usually acquire these pathogens fromfeeding on an infected host, and transmit the pathogens to a naive host during feeding events. To understand the virustransmission dynamics and to survey WNV throughout country, the present study has been conducted. We collected mosquitoesat urban parks in Seongnam, Wonju, Gunsan, Daegu, and Tongyeong using CDC light trap with Dry ice from April toSeptemper in 2017 (mosquito collecting is on going). Among collected mosquitoes, blood-fed mosquitoes were conductedblood meal identification assay and the other mosquitoes were subjected to virus detection using real-time PCR method.A total of 2,290 mosquitoes representing 6 genera and 15 species were collected. The most dominant species was Culexpipiens complex (42.1%) followed by Aedes albopictus (15.1%), Ae. vexans nipponii (14.6%), Ochlerotatus koreicus (9.8%),Cx. orientalis (6.5%), and Armigeres subalbatus (4.4%). The blood meal source were of mammal (93.3%), and birds (6.7%).So far, no WNV has been detected in any mosquitoes.

The members of the genus Flavivirus are noteworthy, as they cause infectious diseases in humans, such as Zika, denguefever, yellow fever, West Nile, and Japanese encephalitis. Due to the increased awareness of the public health risk posedby flavivirus-infected mosquitoes, mosquito collections were performed in six urban parks of South Korea, as the parksare designated for human recreation but also provide suitable habitats for mosquitoes. We examined the diversity andabundance of mosquito species and conducted molecular diagnostics for the detection of flavivirus infections. Monthlycollections were carried out in each park from March to August in 2017. A total of 4,851 mosquitoes (5 genera and13 species) were collected using BG-sentinel traps and then investigated for flavivirus infections. Pathogenic flavivirusinfections causing human diseases were not observed in the field-collected mosquitoes. However, insect-specific flavivirus(ISF) infections were detected in several mosquito pools. ISF has been previously known to enhance or suppress the replicationof medically important flaviviruses in co-infected mosquito cells. In this study, partial sequences of ISF were analyzed.However, further studies are needed in order to determine its genetic characterization and biological function in vivo.

Lentinula edodes are mainly used for cultivation and food in Korea. Consumption is gradually increasing with the characteristic smell and taste. In addition, anti-cancer effect, hypertension, atherosclerosis and other effects have been proven to be a functional food. The cultivation method of highland rice changed from lumber to sawdust bag. Thus, a browned step is required so that the surface of the medium can act like a tree epidermis. The browned medium is not easily contaminated with other bacteria even when in contact with the outside air. It is also a necessary step because it suppresses evaporation of water in the medium and helps mushroom production. But it takes a very long time to browning. Therefore, it is necessary to study the browning of the medium during the cultivation of the altitude. Therefore, the color of the Lab color was measured using a colorimeter to determine the browning efficiency of the medium by LED light source type and intensity. The varieties were Nongjingo and Sanjo-701. The LED light sources were divided into upper, middle, and lower by setting the blue, green, white, and red colors at 100 to 500 ㏓. L values of light source showed the greatest decrease in blue color, followed by white, green and red. It was confirmed that the L value of Sanjo 701 was lower than that of Nongjingo. The decrease of L value by the type of light source showed the same tendency as Nongjingo, and blue was the most decreased, followed by white, green and red.

This study was carried out to develop the technology to manage the growth management of mushroom which was cultivated by long-term knowledge based on the quantified data. In this study, it was developed hardware to monitor and control the growth environment of mushroom cultivation house and an algorithm to automatically grow mushroom. Environmental management for the growth of mushrooms was made possible by cultivation sites, computers and smart phones. In order to manage the environment of mushroom cultivation house, environmental management data of farmers cultivating the highest quality mushrooms in Korea was collected, and a growth management database was created based on the collected data. The management environment for the mushroom cultivation was controlled at ±0.5 °C for temperature, 7 % for upper limit and 3 % for lower limit for relative humidity, and ±10% level for carbon dioxide concentration based on database value. As a result of cultivating mushrooms in such an environment, it was possible to produce mushrooms at almost the same level as those cultivated in farms with the best technology. Therefore, it was considered that the environmental management of the mushroom house managed by the technology based on the long experience could be managed based on the sensor data.

The total mushroom production in Korea in 2015 comprised about 200,000 ton with a value of about 700 billion won (one trillion won if mushroom factory products are included, 1 $=1,128 won). This is almost equal to the amounts generated by flowers and medicinal crops. Even though the cultivation area and the number of farms have been decreased since 2006, the scale of the farms is increasing. Also mushroom productivity and consumption (4.9 ㎏ / person in 2015) are steadily increasing. Major cultivated species are Flammulina velutipes, Pleurotus ostreatus, Pleurotus eryngii, Lentinula edodes and Agaricus bisporus, which cover 90% of the total production. Shelves, logs, plastic bags or plastic bottles are mostly used for cultivation depending on the kind of mushroom. Mushroom export estimated about 43 million $ in 2016. Major exported mushrooms are Flammulina velutipes, Pleurotus eryngii, Lentinula edodes, Pleurotus ostreatus, Tricholoma matsutake and Agaricus bisporus that are destined for the U.S., the Netherlands, Australia, Canada, Hong Kong, Japan and several other countries. Mushroom research in the RDA started in 1967. In the beginning, development of cultivation methods using rice straw was studied together with distribution of improved strains of the button mushroom. The costs of button mushroom production strongly increased due the improving national economy, shortage of workers, increase of wages and the oil crisis in the nation. This caused a weak competitive position for button mushroom export and resulted in reduction of the button mushroom cultivation area. After development of a cotton waste cultivation method for the oyster mushroom (Pleurotus ostreastus), this mushroom was popularized and mainly studied. Development of automated plastic bottle cultivation systems in the 1990s changed shelf cultivation to mass production. The bottle system was applied to the oyster mushroom but also to F. velutipes and P. eryngii. Mushroom research in the RDA nowadays focuses on analysis of mushroom genetics in combination with development of new mushroom varieties, mushroom physiology and cultivation. Further studied are environmental factors for cultivation, disease control, development and utilization of mushroom substrate resources, post-harvest management and improvement of marketable traits. Finally, the RDA manages the collection, classification, identification and preservation of mushroom resources.

This study was carried out to explore possibilities of cultivating horticultural crops in the air-dome greenhouse in comparison to the common iron-frame greenhouse as the standard. The levels of carbon dioxide and atmospheric pressure measured inside the air-dome greenhouse turned out to be higher than those measured inside the iron-frame greenhouse. Contrastingly, light intensity was relatively weaker inside the air-dome greenhouse due to the air-inflated double layers. Plants of melon and cherry tomato were cultivated from May 2 to August 12, 2016, respectively in the two greenhouses. For melon plants, growth in the air-dome greenhouse effectively increased fruit weight as well as trunk circumference compared to iron-frame greenhouse. Moreover, soluble sugar content of melon fruit was significantly higher when cultivated in the air-dome greenhouse. For cherry tomato plants, fruit yield of cherry tomato was significantly increased inside the air-dome greenhouse. Furthermore, it has been found that the air-dome greenhouse was considerably effective in shortening the growing period of melon and cherry tomato plants in comparison to the iron-frame greenhouse.

consumption, has been attempted to replace with sharing economy which is consumer-to-consumer’s activity of obtaining, giving, or sharing the access to goods and services (Hamari et al., 2016). Research argued that the sharing economy develops based on information and communication technology (ICT) as it is an emerging economic-technological phenomenon, proliferation of using social networking site (SNS) (e.g. Instagram, Facebook, YouTube), and increased consumer awareness (Belk, 2014; Hamari et al., 2016). SNS refers to a digital environment that allows individual to create his/her space where sharing and constructing relationship with others are possible (Lin & Lu, 2011). Among the numbers of SNSs, Instagram is gaining notable attention as powerful marketing tool which may especially be important for fashion industry. Hutchins (2017) reported that number of its daily active users exceeds 400 million, and 90% of users are under 35 who may be familiar with the idea of the sharing economy. In terms of marketing, 53% of Instagram users are found to follow their favorite brands (Hutchins. 2017). According to Ryan and Deci (2000), consumer behavior of using information technology such as Instagram was influenced by extrinsic and intrinsic motivations. Although numerous researches have done with the sharing economy and SNS respectively, despite the recognized role of SNS in the sharing economy, comprehensive and empirical study of the sharing economy and SNS is very limited. Hence, the purpose of this research is to investigate consumer’s motivations to use Instagram for participating sharing economy by developing research framework based on the motivation theory (Ryan & Deci, 2000) perspective.

Rubus coreanus is known to have diverse biological properties, such as free radical scavenging activity and antibacterial activity. In the present study, Rubus coreanus leaf and stem extract (RLSE) was used in boar semen preservation whether it has a beneficial effect on assisted reproductive technology (ART) in mammals. Boar spermatozoa were preserved in Beltsville thawing solution (BTS) in the presence of varying concentrations of RLSE (0-10 μg/mL). Sperm motility, sperm viability, and intracellular reactive oxygen species (ROS) levels were examined after 2 days of preservation. The percentage of total motile spermatozoa and progressive motile spermatozoa improved in the spermatozoa preserved with 0.5 μg/mL RLSE. Higher proportions of viable spermatozoa were seen in the presence of 0.5 and 1 μg/mL RLSE than in the control. Intracellular ROS levels decreased when the spermatozoa were preserved in BTS with 0.1–1 μg/mL RLSE. In order to examine the bacterial growth, E. coli was added to liquid semen diluted with antibiotics-free BTS in the presence or absence of RLSE. No anti-bacterial activity of RLSE against E. coli was observed during liquid semen preservation. Although there was no inhibition of E. coli growth, the addition of RLSE might help improve sperm motility and viability during boar semen preservation, suggesting it as a potential reagent for ART in mammals.

Mesenchymal stem cells (MSCs) have been considered an alternative source of neuronal lineage cells, which are difficult to isolate from brain and expand in vitro. Previous studies have reported that MSCs expressing Nestin (Nestin+ MSCs), a neuronal stem/progenitor cell marker, exhibit increased transcriptional levels of neural development-related genes, indicating that Nestin+ MSCs may exert potential with neurogenic differentiation. Accordingly, we investigated the effects of the presence of Nestin+ MSCs in bone-marrow-derived primary cells (BMPCs) on enhanced neurogenic differentiation of BMPCs by identifying the presence of Nestin+ MSCs in uncultured and cultured BMPCs. The percentage of Nestin+ MSCs in BMPCs was measured per passage by double staining with Nestin and CD90, an MSC marker. The efficiency of neurogenic differentiation was compared among passages, revealing the highest and lowest yields of Nestin+ MSCs. The presence of Nestin+ MSCs was identified in BMPCs before in vitro culture, and the highest and lowest percentages of Nestin+ MSCs in BMPCs was observed at the third (P3) and fifth passages (P5). Moreover, significantly the higher efficiency of differentiation into neurons, oligodendrocyte precursor cells and astrocytes was detected in BMPCs at P3, compared with P5. In conclusion, these results demonstrate that neurogenic differentiation can be enhanced by increasing the proportion of Nestin+ MSCs in cultured BMPCs.

For the development of processed food for hypoglcemia, it is important to construct model system to confirm factors that reduce the glycemic index in real food. This study was to investigate the relationship between the gel model system and real food the high amylose type of segoami using response surface methodology. The independent variables were concentration (X1; 10, 15, 20, 25, and 30%) and steaming time (X2; 30, 35, 40, 45, and 50 min). The predicted glycemic index (Y1) was analyzed as a dependent variable. The regression of pGI was 0.7343, indicating that the model fits the data well in the prediction test. The predict glycemic index of the gel was in the range of 71.38 ~ 83.78, depending on the gel preparation conditions and predicted optimum condition was 23.7% gel concentration and 43.8 minutes of gelatinization. The predict glycemic index of rice gel were decreased with an increase of gel concentration, rather than the steaming time. In order to confirm the effect on in vitro digestibility in real food, the real food (Garraedduk) was prepared by applying the optimal conditions. As a result, there was no significant difference between the predicted value (77.1) and the experiment value (76.6). This result showed that the in vitro digestibility in real food can be predicted by applying the gel model system.

Evaluation of the antioxidant activity of the bakery products is important in order to establish healthy consumption patterns. Aronia and blackberry extract at different levels (0%, 5%, 10%, 15%, and 20%) were used to investigate their comparative effects on the physical properties and antioxidant capacity of cookies. The final cookies were evaluated for physicochemical and sensory properties as well as total phenol content, DPPH radical scavenging activity, and FRAP assay. Substitution of aronia and blackberry extracts significantly (p<0.05) increased the moisture content and spread ratio compared to the control that did not contain the extract. However, types of the extract (aronia or blackberry) did not significantly affects the moisture and spread ratio of cookies at the same concentrations (p>0.05). The lightness and yellowness values of cookies were decreased while the redness value was increased by the amount of the extract compared to control (p<0.05). For textural properties, the hardness and chewiness of cookies were increased by the concentration of extract, and highest at 20% of both extracts. Polyphenol content in the cookies was increased with the concentration of the extract added into the cookies. Consistently, the antioxidant activity was significantly increased as the concentration of the extract was increased (p<0.05). Cookies with aronia extract showed higher antioxidant activity compared to that with blackberry (p<0.05), due to the presence of higher content of polyphenol. In terms of appearance, flavor, taste, texture and overall acceptability, the sensory evaluation scores of cookies with 20% aronia and 10% blackberry were significantly higher compared to the control (p<0.05). Based on above result, using more than 10% of the aronia or blackberry extracts would appropriate for making cookies.