Exudative epidermitis (EE) is a generalized skin disease of pigs, mainly caused by Staphylococcus hyicus (S. hyicus). Antibiotic resistant S. hyicus leads to the failure of antimicrobial treatments. This necessitates proper identification of the strains in the field and determination of their antimicrobial susceptibility. This study was carried out to isolate Korean S. hyicus and determine its antimicrobial resistance. Isolate was sensitive to ceftiofur, ciprofloxacin, enrofloxacin, gentamicin, neomycin, and tylosin, but remarkably resistant to amoxicillin, lincomycin, penicillin, streptomycin, and tetracycline. Our study contributes to the understanding of the characteristics and antimicrobial resistance of Korean S. hyicus, which in turn will provide an antimicrobial treatment strategy to control EE.
In the world of SNS, once ordinary users get recognized for discriminated fashion taste, they affect other users’ choice of fashion products. Fashion companies capture the importance of these influencers and tries to take advantage of their ability. However, the benefits offered to influencers such as financial compensation may provoke negative consumer reactions. The purpose of this study is to identify what aspects of SNS influencers and how they influence other fashion consumers. We suggest that influencers’ self-disclosure and authenticity of posts are important factors which elicit positive response from consumers. We also investigate the psychological mechanism of envy which can mediate the impact of self-disclosure and authenticity on consumer response. This study was conducted by an online survey of 120 Korean women in their 20s and 30s with a 2 x 2 between-group experimental design. The data was collected from 28 November to 5 December 2016. The collected data was analyzed using SPSS 23.0 for ANOVA analysis and regression analysis.
The results were as follows. The main effect of authenticity was confirmed, while self-disclosure did not play a significant role, which indicates authenticity is the main factor in exercising influence on consumer response. The interaction of authenticity and self-disclosure was also observed. The participants of high in both self-disclosure and authenticity showed the strongest consumer reaction. However, the participants of high self-disclosure and low authenticity induced the weakest consumer response. For the impacts of self-disclosure and authenticity on envy, the participants exposed to the condition of both high self-disclosure and authenticity showed benign envy. On the other hand, malicious envy was aroused when both self-disclosure and authenticity were low. In relations between authenticity and consumer response, partial mediating effect of benign envy and malicious envy was confirmed. But, there was no mediating effect between self-disclosure and consumer response.
This research on consumer response to influencers’ posts will provide key implications to fashion companies in building marketing strategies. In order for influencers' posts displaying their daily lives to have a positive impact on consumer response, not only self-disclosure but authenticity has to be considered. Such posts can induce benign envy and subsequently impact consumers indirectly. Influencers’ posts which narrow the gap between daily lives and products and raise the authenticity are important keys to successful marketing.
Technology is changing the way of navigation. New technologies for communication and navigation can be found on virtually every vessel. System architectures define structure and cooperation of components and subsystems. IMO, IALA, costal authorities, technology provider and many more actually propose new architectures for e-Navigation. This paper looks at other transportation domains and technical as normative requirements for e-Navigation architectures. With the aim of identifying possible synergies in the research, development, certification and standardization, this paper sets out to compare requirements and approaches of these two domains with respect to safety and security aspects. Since from an autonomy perspective, the automotive domain has started earlier and therefore has achieved a higher degree of technical progress, we will start with an overview of the developments in this domain. After that, the paper discusses the requirements on automation and assistance systems in the maritime domain and gives an overview of the developments into this direction within the maritime domain. This then allows us to compare developments in both domains and to derive recommendations for further developments in the maritime domain at the end of this paper.
Impact damages induced by a low-velocity impact load on carbon fiber reinforced polymer (CFRP) composite plates fabricated with various stacking sequences were studied experimentally. The impact responses of the CFRP composite plates were significantly affected by the laminate stacking sequences. Three types of specimens, specifically quasi-isotropic, unidirectional, and cross-ply, were tested by a constant impact carrying the same impact energy level. An impact load of 3.44 kg, corresponding to 23.62 J, was applied to the center of each plate supported at the boundaries. The unidirectional composite plate showed the worst impact resistance and broke completely into two parts; this was followed by the quasi-isotropic lay-up plate that was perforated by the impact. The cross-ply composite plate exhibited the best resistance to the low-velocity impact load; in this case, the impactor bounced back. Impact parameters such as the peak impact force and absorbed energy were evaluated and compared for the impact resistant characterization of the composites made by different stacking sequences.
Insect and natural enemy fauna were surveyed at conventional (CV) and organic-farming persimmon orchards (OF) at Jinju, Korea, using sweeping net and naked eyes in 2013 and 2014. Using sweeping net, 49 species at an OF and 18 species at a CV were observed in 2013. In 2014 too, more species were observed at OF (66 species, 33 families, 7 orders) than at a CV (25 species, 15 families, 5 orders). During both the years, dominant species were all hemipteran insects like Nysius plebejus (Lygaeidae) and Cletus punctiger (Coreidae) in both types of orchards, followed by Rhopalus maculatus (Rhopalidae), Riptortus pedestris (Alydidae). Similarly, naked eye inspection also found more species at OF (192 species, 72 families, 11 orders) than at CV (104 species, 52 families, 10 orders) in 2014. Dominant species in naked eye inspection were Lycorma emelianovi (Hemiptera: Fulgoridae), Apis mellifera (Hymenoptera: Apidae), Uroleucon cephalonopli (Hemiptera: Aphididae) at OF, and A. mellifera and R. maculatus at CV in 2014. Using naked eye inspection or sweeping net in 2014, more species of natural enemies were observed at OF (31 and 9 species, respectively) than at CV (18 and 5 species, respectively).
Carbon aerogel is a porous carbon material possessing high porosity and high specific surface area. Nitrogen doping reduced the specific surface area and micropores, but it furnished basic sites to improve the CO2 selectivity. In this work, N-doped carbon aerogels were prepared with different ratios of resorcinol/melamine by using the sol-gel method. The morphological properties were characterized by scanning electron microscopy (SEM). Nitrogen content was studied by X-ray photoelectron spectroscopy (XPS) and the specific surface area and micropore volume were analyzed by N2 adsorption-desorption isotherms at 77 K. The CO2 adsorption capacity was investigated by CO2 adsorption-desorption isotherms at 298 K and 1 bar. Melamine containing N-doped CAs showed a high nitrogen content (5.54 wt.%). The prepared N-doped CAs exhibited a high CO2 capture capacity of 118.77 mg/g (at resorcinol/melamine = 1:0.3). Therefore, we confirmed that the CO2 adsorption capacity was strongly affected by the nitrogen moieties.
A partial sequence of the mitochondrial cytochrome oxidase subunit I (COI) gene is widely used as a molecular marker for species identification in animals, also termed a DNA barcode. However, the presence of more than one sequence type in a single individual, also known as heteroplasmy, is one of the shortcomings of barcode identification. In this study, we examined the extent and divergence of COI heteroplasmy, including nuclear-encoded mitochondrial pseudogenes (NUMTs), at the genomic-DNA level from 13 insect species, including four individuals of orthopteran Anapodisma miramae. Furthermore, a long fragment of mitochondrial DNA (~13.5 kb) and cDNA from A. miramae were used as a template for COI PCR to compare the patterns of heteroplasmy between DNA sources and to investigate a possible way to avoid ambiguity in DNA barcoding. When multiple numbers of clones originated from genomic DNA were sequenced, heteroplasmy was prevalent in all species (3~16 heteroplasmic copies), with a varying degree of maximum sequence divergence (<1% in 7 species, <4% in 3 species, <6% in 2 species and 2.15-8.03% in four A. miramae individuals). In five species, NUMTs also were observed when genomic DNA was used as a template. Long fragment DNA also is a source of heteroplasmic amplification, but the divergent haplotypes and NUMTs obtained in the genomic DNA-based PCR were not detected in A. miramae. On the other hand, cDNA was heteroplasmy-free, without NUMTs when multiple numbers of clones were sequenced. Consistently, one dominant haplotype was always obtained from the genomic DNA-origin clones in all species and also from the long fragment- and cDNA-origin clones of A. miramae. Furthermore, the dominant haplotype was identical in sequence, regardless of the DNA source. Thus, one possible solution to avoid the barcoding problem in relationship to heteroplasmy could be the acquisition of multiple numbers of barcoding sequences to determine a dominant haplotype that can be assigned as barcoding sequence for a given species.
A 2-year-old intact female pomeranian dog presented dyspnea, labored breathing, cough, exercise intolerance, machinery heart murmur, and precordial thrill. A left-to-right patent ductus arteriosus (PDA) was diagnosed based on two-dimensional echocardiography, thoracic radiography, electrocardiography, and blood work. An angiography was performed to accurately evaluate the size and shape of the duct. An interventional approach for transcatheterial occlusion of the PDA was achieved using an Amplatz® Canine Duct Occluder (ACDO), which is a commercially available ductal occluding device. Due to the limited size of the dog’s femoral artery, a device smaller [125% of minimal ductal diameter (MDD); recommended size: 150~200% of MDD] than recommended was mounted. After placement of the ACDO, precordial thrill and continuous heart murmur disappeared, and the patient was discharged the next day after stabilization with O2 supply. Upon follow up examination, dyspnea, labored breathing, cough, exercise intolerance, and cardiomegaly were improved with no complications after the procedure. The ACDO was well maintained in position. This case represents successful clinical application of the Amplatz® Canine Duct Occluder to achieve closure of a PDA using a slightly smaller device than the recommended size.
We investigated the change of growth characters by over-expressing OsPT6 (OsPT6-OX) under phosphorus (Pi) deficient condition in rice. In analyzing the change of growth rate according to Pitreatment for two weeks, shoot growth rate of OsPT6-OX was increased in Pi deficient condition than in Pi sufficient condition. Therefore, the chlorophyll content of leaf in OsPT6-OXs did not increase even decreased in the youngest leaf. However growth rate of wild type plant in Pi deficient condition showed significant reduction with increment of chlorophyll content. In analyzing agronomic characters, the tiller number of OsPT6-OX less decreased than those of wild type plant in Pi deficient condition. Panicle number and ripening rate of wild type plant were changed according to Pi treatment. However those of OsPT6-OX did not change in such conditions. Resulting of changes in agronomic characters, yield of wild type plant decreased about 10 % in Pi deficient condition. That of OsPT6-OX did not show significant difference in Pi deficient condition. The results obtained this study demonstrated that over-expression of OsPT6 could increase phosphorus deficient stress resistance in rice.
Lacticin NK34 is a small nisin-like bacteriocin present in the supernatants of an isolate of Lactococcus lactis from jeotgal, a salted and fermented Korean food made with seafood such as shrimp, oysters, and fish. Recently, we demonstrated that a partially purified NK34 is highly effective against various Staphylococcus species in a murine infection model. In this study, the two major bacterial pathogens associated with bovine mastitis, Streptococcus aureus and S. agalactiae, were evaluated for their susceptibility to NK34 in vitro using a standard teat-dip assay as well as in vivo using mastitic cows infected with one of these pathogenic strains. The experimental analyses showed a significant decrease (up to 98 times) in the bacterial numbers between the NK34-treated and -untreated teats. Moreover, a dramatic reduction in somatic cell counts was observed at 3 days post-treatment with 10 ml of NK34 administered directly into the mastitic cows. Neither S. aureus nor S. agalactiae were recovered. Taken together, these results imply that lacticin NK34 is an alternative antimicrobial substitute for the treatment of bovine mastitis, caused especially by either S. aureus or S. agalactiae.
Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of -2.5°C, 0°C, 2.5°C and 5°C with constant humidity >80%, the queens stored at 2.5°C exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at 0°C, 5°C, 7.5°C and 12.5°C. At 2.5°C the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at 5°C. Thus, 2.5°C and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.