Incursions of red imported fire ant (RIFA), Solenopsis invicta into Korea have been increasing. After a first interception of a colony of S. invicta on Gamman pier, Pusan port while intensive surveillance by Animal & Plant Quarantine Agency (APQA) in September 2017, three more RIFA colonies have been found in sea port piers of Pyeongtek, Incheon and Pusan cities. The social forms and mitochondrial DNA haplotypes of the intercepted RIFA colonies were analysed by allelic discrimination assay (peptide nucleic acid probe based RT-PCR) of Gp-9 gene and mt-DNA fragment of 768 bp, which is part of the Cytochrome oxidaseⅠ gene. The colony on Gamman pier, intercepted in Sep. 2017 was previously reported as a haplotype 5 (H5) of mitochondrial DNA and a social form of polygyne. The colony on Hutchison pier of Pusan port, intercepted in June 2018 were confirmed as a H22 haplotype and a monogyne. Those different social forms show different origins of each colonies. Those on piers of Pyeongtek and Incheon ports, also found in 2018 were confirmed samely as H22 and monogyne. However, it could be putatively assumed that those two colonies were differently introduced via different container cargoes, considering those colonies were found in container yards of distantly located different sea ports. More genetic variation analyses using diverse sets of molecular markers such as microsatellites, genome-wide single nucleotide polymorphisms, etc. in nuclear gens are being proceeded for more exact introduction routes (origins).

벼메뚜기는 분류학적으로 메뚜기목(Orthoptera), 메뚜기과(Acrididae)에 속하는 곤충으로 작물에 피해를 주는 해충으로 인식하고 있지만 예로부터 단백질 보충을 위하여 채집하여 튀기거나 볶음요리 로 이용해 왔다. 벼메뚜기는 갈색거저리, 쌍별귀뚜라미 등과 함께 식품공전에 등록되어 있으나 1년 1세대로 가을철에 채집하여 이용하는 실정으로 공급확대에 한계가 있다. 최근 우리나라에서 벼메뚜기를 사육하고자 하는 농가와 다양한 용도로 가공을 통해 이용하려는 수요가 점차 증가하고 있으나 연중사육기술이 개발되지 않아 대량사육하는 농가가 거의 없는 실정이다. 이에 벼메뚜기의 연중대량사육기술 뿐만 아니라 인공사료 및 자동화 사육시설 개발에 따른 생산비 절감 기술 개발이 필요한 실정이다. 벼메뚜기의 먹이로는 여름작물로 옥수수, 수단그래스, 겨울작물로 밀, 보리 등의 볏과작물이 있으며 많은 양의 생엽과 발육에 적합한 작물의 선발 및 볏과작물의 통곡실 가루를 이용한 인공사료의 개발로 먹이공급 부족 시 15~20일 급여 가능하며, 벼메뚜기 사육시설로 단독형, 연결형, 절충형 등의 사육시설을 개발하여 연결형 사육시설의 수확량과 생존율이 가장 높았으며, 예취 급이 노동력이 단독형의 16% 소요되었다.

The purpose of this study was to investigate the effect of heel off stairway walking exercise on the increase of muscle activity and balance activity of the ankle joint muscles in university students with functional ankle instability. The conservative treatment for the control group consisted of stairway walking (n=10) and the experimental group consisted of heel off stairway walking (n=11). The therapeutic intervention of the control and experimental groups was performed a total of 12 exercise sessions, 3 times per week for 4 weeks. To compare the two groups, the level of ankle disability was assessed by using the EMG, BT4 and Pedoscan in pre-treatment and post-treatment. Muscle activity increased in both the experimental groups and control groups in each group, however there was no significant difference between the groups. Balance ability did not show any significant difference. This study demonstrates that heel-off stairway walking is effective in significantly increasing muscle activity, however did not significantly improve balance ability.

이 연구는 CHO-CO와 PO필름이 시설 내 기온과 참 외의 특성, 수량에 미치는 영향을 구명하고자 수행되었 다. 2017년 1월 8일 기온의 일변화 조사에서 조광필름 처리구의 최고, 최저 및 평균 기온은 각각 38.9oC, 13.4oC, 20.1oC 이었고, PO필름 처리구에서 각각 40.0oC, 14.9oC, 20.3oC로 처리 간 차이가 거의 없었고, 2월부터 5월까지 과실특성, 품질 및 수량 조사에서도 처 리 간 차이가 없었다. 그러나 고온기인 8월 7일부터 1일 간 기온의 일변화에서 조광필름 처리구의 최고, 최저 및 평균 기온은 각각 47.2oC, 23.1oC, 32.4oC 이었고, PO필 름 처리구에서도 각각 50.3oC, 23.6oC, 34.0oC로 조광필름 처리구에서 최고 및 평균기온이 각각 3.1oC, 1.6oC 더 낮았다. 특히 주간의 기온만 비교해 보면 저온기에는 차이가 거의 없지만 고온기에는 PO필름에 비해 조광필 름 처리구에서 3.0oC까지 기온이 낮아지는 것을 알 수 있었다. 2017년 5월 26일부터 8월 15일까지의 고온기의 품질 및 수량 조사에서도 PO필름 처리구에 비해 조광필 름 처리구에서 과중이 371.6g으로 22.2g 더 가볍고, 태좌부의 당도는 14.5o brix로 1.4o brix 더 높고, 과피의 색도(a값)도 12.3으로 1.5 더 높고, 상품과율도 89.4%로 8.0%P 증가하였고 10a당 상품수량도 2,694kg으로 26% 증가하였다. 결론적으로 고온기에 조광필름은 참외 생산에 효과적으로 이용될 수 있을 것이다.

Porcine spermatogonial stem cells (SSCs) prefer three-dimensional (3D) culture systems to 2D ones for the maintenance of self-renewal. Of the many 3D culture systems, agar-based hydrogels are candidates for supporting porcine SSC self-renewal, and there are various types of agar powder that can be used. In this study, we sought to identify an agar-based 3D hydrogel system that exhibited strong efficacy in the maintenance of porcine SSC self-renewal. First, 3D hydrogels with different mechanics were prepared with various concentrations of Bacto agar, lysogeny broth (LB) agar, and agarose powder, and the 3D hydrogel with the strongest alkaline phosphatase (AP) activity and greatest increase in colony size was identified for the different types of agar powder. Second, among the porcine SSCs cultured in the different 3D hydrogels, we analyzed the colony formation, morphology, and size; AP activity; and transcription and translation of porcine SSC-related genes, and these were compared to determine the optimal 3D hydrogel system for the maintenance of porcine SSC self-renewal. We found that 0.6% (w/v) Bacto agar-, 1% (w/v) LB agar-, and 0.2% (w/v) agarose-based 3D hydrogels showed the strongest maintenance of AP activity and the most pronounced increase in colony size in the culture of porcine SSCs. Moreover, among these hydrogels, the strongest transcription and translation of porcine SSC-related genes and largest colony size were detected in porcine SSCs cultured in the 0.2% (w/v) agarose-based 3D hydrogel, whereas there were no significant differences in colony formation and morphology. These results demonstrate that the 0.2% (w/v) agarose-based 3D hydrogel can be effectively used for the maintenance of porcine SSC self-renewal.

Si3N4 is a ceramic material attracting attention in many fields because of its excellent abrasion resistance. In addition, Ti and TiAl alloys are metals used in a variety of high temperature environments, and have attracted much attention because of their high strength and high melting points. Therefore, study of the interface reaction between Si3N4 / Ti and Si3N4 / TiAl can be a useful practice to identify phase selection and diffusion control. In this study, Si3N4 / Ti5Si3 + TiN / TiN / Ti diffusing pairs were formed in the Si3N4 / Ti interfacial reaction and Si3N4 / TiN(Al) / Ti3Al / TiAl diffusion pathway was identified in the Si3N4 / TiAl interfacial reaction. The diffusion layers of the interface reactions were identified and, to investigate the kinetics of the diffusion layer, the integrated diffusion coefficients were estimated.

The relationship of in vitro starch digestibility and gel strength was investigated at various concentrations (10-30%) of rice cultivars with different amylose contents (27.9, 17.9, and 5.2%). As the rice flour concentration increased, predicted glycemic index decreased, but gel strength increased regardless of amylose contents. Gel strength correlated strongly with amylose content, whereas in vitro starch digestibility was more highly affected by rice flour concentration than by amylose contents. Moreover, the impact of degree of gelatinization on in vitro starch digestibility of high amylose rice was also examined in terms of structural features and rheological properties. The digestion rate of fully gelatinized flour was 1.7 times higher than that of native flour, while the disrupted structure with a different gelatinization degree during starch digestion was visually demonstrated through the X-ray diffraction and molecular distribution analysis. The rice flour changed from an A-type to a V-type pattern and showed difference in crystalline melting. The low molecular weight distribution increased with increasing degree of gelatinization during starch digestion. The apparent viscosity also increased with degree of gelatinization. These results demonstrated that the starch digestibility of rice was more affected by concentration than by amylose content, as well as by the degree of gelatinization due to structural difference.

The members of the genus Flavivirus are noteworthy, as they cause infectious diseases in humans, such as Zika, denguefever, yellow fever, West Nile, and Japanese encephalitis. Due to the increased awareness of the public health risk posedby flavivirus-infected mosquitoes, mosquito collections were performed in six urban parks of South Korea, as the parksare designated for human recreation but also provide suitable habitats for mosquitoes. We examined the diversity andabundance of mosquito species and conducted molecular diagnostics for the detection of flavivirus infections. Monthlycollections were carried out in each park from March to August in 2017. A total of 4,851 mosquitoes (5 genera and13 species) were collected using BG-sentinel traps and then investigated for flavivirus infections. Pathogenic flavivirusinfections causing human diseases were not observed in the field-collected mosquitoes. However, insect-specific flavivirus(ISF) infections were detected in several mosquito pools. ISF has been previously known to enhance or suppress the replicationof medically important flaviviruses in co-infected mosquito cells. In this study, partial sequences of ISF were analyzed.However, further studies are needed in order to determine its genetic characterization and biological function in vivo.

본 연구는 히터팬 처리에 의한 시설환경 개선 및 참외의 품질 및 수량성을 검정코자 수행하였다. 히터팬 처리에 따른 2017년 1월 1일부터 31일간 터널내부의 일평균 온도는 무처리구의 17.8oC에 비하여 히터팬 처리구에서는 18.7oC로 히터팬 처리구에서 0.9oC 더 높았다. 작물이 자라고 있는 지상 20cm 부위의 풍속은 무처리구의 0.05m·s-1에 비해 히터팬 처리구에서 0.24m·s-1로 4.8배 높았고 온도는 무처리구의 35.3oC에 비해 히터팬 처리구에서는 40.9oC로 히터팬 처리구에서 5.6oC 더 높았으나 습도는 무처리구의 39.1%에 비해 8.1%P 더 낮았다. 정식 51일후 개화율 조사에서 무처리구의 91%에 비하여 히터팬 처리구에서는 96%로 5%P 더 높았고, 첫 수확 소요일수도 무처리구의 86일에 비해 4일 단축되었다. 과 중은 무처리구의 339.7g에 비하여 히터팬 처리구에서 15.1g 더 무거웠고 상품과율도 90.4%에 비하여 3.4%P 더 많았으며 10a당 상품수량도 142.9kg에 비하여 38% 더 증가하여 95% 수준에서 통계적 유의성이 인정되었다. 이상의 결과를 종합 하면 히터팬 처리로 시설내 온도 상승은 물론 공기의 유동이 활발하여 작물생육에 유리하고 수확시기가 단축되고 품질이 향상되고 수량이 증가하는 것을 알 수 있었다.

Selecting an appropriate antigen with optimal immunogenicity and physicochemical properties is a pivotal factor to develop a protein based subunit vaccine. Despite rapid progress in modern molecular cloning and recombinant protein technology, there remains a huge challenge for purifying and using protein antigens rich in hydrophobic domains, such as membrane associated proteins. To overcome current limitations using hydrophobic proteins as vaccine antigens, we adopted in silico analyses which included bioinformatic prediction and sequence-based protein 3D structure modeling, to develop a novel periodontitis subunit vaccine against the outer membrane protein FomA of Fusobacterium nucleatum. To generate an optimal antigen candidate, we predicted hydrophilicity and B cell epitope parameter by querying to web-based databases, and designed a truncated FomA (tFomA) candidate with better solubility and preserved B cell epitopes. The truncated recombinant protein was engineered to expose epitopes on the surface through simulating amino acid sequence-based 3D folding in aqueous environment. The recombinant tFomA was further expressed and purified, and its immunological properties were evaluated. In the mice intranasal vaccination study, tFomA significantly induced antigen-specific IgG and sIgA responses in both systemic and oral-mucosal compartments, respectively. Our results testify that intelligent in silico designing of antigens provide amenable vaccine epitopes from hard-to-manufacture hydrophobic domain rich microbial antigens.

Generally, fate of spematogonial stem cells (SSCs) can be determined specifically by microenvironments enclosed with various extracellular matrix (ECM) components and integrins recognizing directly ECM proteins play an pivotal role in transporting ECM-derived signals into cytoplasm, resulting in inducing a variety of biological functions such as cell attachment, self-renewal and differentiation. However, to date, studies on type of integrins expressed on the undifferentiated SSCs remain unclear. Therefore, we tried to investigate systematically what kind of integrin subunits are expressed transcriptionally or translationally in the SSCs derived from testis of hybrid B6CBAF1 mouse. For these, isolation of SSCs from testis were conducted by magnetic activated cell sorting (MACS) using Thy1 antibody. Subsequently, transcriptional and translational level of integrin α and β subunits in the isolated SSCs were measured by real-time PCR and fluorescene immunoassay, respectively. As the results, transcriptional levels of genes encoding total 25 integrin subunits were quantified, and integrin α4, α6, α7, α9, αV, αL and αE and integrin β1, β5 showed higher expression levels than other subunits. By contrast, integrin α3, α5, α 10 and α11 and integrin β2, β3, β4, β7 were weakly transcribed. When translational levels of the integrin α subunits showing high transcription level (α4, α6, α7, α9, αV αL, and αE) were measured, integrin α6, α7, α9, αV and αL were higher than integrin α4 and αE. In case of integrin β subunit, β1 evaluated more expression than β5. From these results, we speculate that the undifferentiated SSCs derived from hybrid B6CBAF1 mouse may express integrin α4β1, α6β1, α7β1, α9β1, αVβ1 and/or αVβ5 on plasma membrane. Moreover, this information will greatly contribute to constructing non-cellular niche supporting self-renewal of SSCs in the future.

A study was conducted to determine the rheological properties of pomegranate puree (PP) as affected by different gums (xanthan and guar gums) and temperatures (25, 35 and 45°C). The rheological properties of the samples were determined using a rotational rheometer at a shear range of 1 to 40 s-1. The PP added with xanthan and guar gums were found to be Non-Newtonian fluids following the Herschel-Bulkley model. The yield stress and consistency coefficient of the PP with xanthan gum at different temperatures were higher than those of the PP with guar gum but the opposite was observed for the flow behavior index. Moreover, all rheological properties of the PP regardless of type of gum addition decreased with increasing temperature. The consistency coefficient was related to temperature using an Arrhenius-type relationship. The PP with xanthan gum (16.11 kJ/mol) has higher activation energy than the PP with guar gum (11.44 kJ/mol). The yield stress values of the PP with xanthan gum and guar gum can be related to temperature by linear regression equations and the flow behavior index values by exponential regression equations.

참외 육묘 시설 내에서 우수한 품질의 모종을 생산하기 위한 LED 광원의 이용 가능성을 검토하기 위해 접목 활착 후 20일 동안 광원과 광도를 달리하여 묘소질과 정식 후 생육을 비교하였다. 광원은 청색광(B), 적청색 혼합광원(RB3, RB7)을 이용하였고, 광도(PPFD)는 50, 100, 200μmol·m-2·s-1로 처리하였다. 조명시간은 일출 (7:30) 전 2시간과 일몰(17:30) 후 2시간씩 하루에 총 4 시간을 처리하는 일장연장법을 이용하였다. 참외 지상부 의 생장지표인 접수 길이와 줄기직경은 청색광의 비율이 높을수록 길어지고 굵어지는 경향을 나타내었다. 적청색 혼합광원(RB3)이 다른 광원들에 비해 건물률과 조직의 충실도가 높은 경향이었다. 광합성률은 적색광의 비율이 높을수록 증가하였으며, RB7 200μmol·m-2·s-1처리구에서 5.44μmolCO2·m-2·s-1로 가장 높았다. 정식 후 RB3 200μmol·m-2·s-1 처리구의 초장이 132.3cm로 가장 길었고, 마디수가 22.7개로 가장 많았으며 개화율도 75%로 가장 높았다. 청색광(B) 단독으로 처리한 것보다는 적청색 혼합광원(RB3)으로 처리한 것이 묘소질을 양호하게 하였고, 광도를 200μmol·m-2·s-1로 높이는 것이 우량묘 생산에 유리할 것으로 판단되었다.

A study was conducted to determine the rheological properties of gochujang and chogochujang at different temperatures (25, 35 and 45°C). The rheological properties of the samples were determined using a rotational rheometer at a shear range of 1 to 40 s-1. The gochujang and chogochujang were found to be Non-Newtonian fluids following the Herschel-Bulkley model. The yield stress and consistency coefficient of the gochujang at different temperatures were higher than those of the chogochujang but the opposite was observed for the flow behavior index. Moreover, all rheological properties of the gochujang and chogochujang decreased with increasing temperature. The consistency coefficient was related to temperature using an Arrhenius-type relationship. The gochujang (14.48 kJ/mol) has higher activation energy than the chogochujang (14.03 kJ/mol). The yield stress values of the gochujang and chogochujang can be related to temperature by linear regression equations and the flow behavior index values by exponential regression equations.

PO 필름 공급이 경북 성주지역 참외 산업에 미치는 영향을 분석하기 위하여 참외 재배면적, 재배 농가수, 수량 및 소득 등을 분석한 결과, PE 필름은 2010년에 비해 2014년도에는 전국, 경북 성주 지역에서 각각 91.5%, 94.9%, 95.2%로 감소한 반면, PO 필름 보급은 각각 342.5%, 500%, 825% 이었다. 2010년에 비해 2015년 재배면적과 농가수는 각각 8.8%, 11.9% 감소하였다. 수량과 생산량은 각각 38.2%, 25.9% 증가하였다. 조수입은 2010년 3,051 억원에서 2015년 4,020 억원으로 1.3배 증가하였고 소득도 2010년 1,936 억원에서 2015년 4,020 억원으로 2.7배 증가하였다. 이상의 결과를 종합 하면 PO 필름으로 교체되면서, 참외 생산성은 증대되고, 작업 노력 및 유해물질 배출은 감소될 것으로 보여진다.

The feasibility of incorporating licorice powder (LP) as a value-added food ingredient into convenient food products was investigated using yanggaeng as a model system. LP was incorporated into yanggaeng at amounts of 0, 2, 4, 6, and 8% (w/w) based on total weight of cooked white beans and LP. pH level decreased while moisture content and soluble solid content increased significantly with increasing levels of LP (P<0.05). In terms of color, lightness decreased while redness and yellowness increased significantly (P<0.05) with increasing levels of LP. Hardness also decreased significantly with higher amount of LP in the formulation (P<0.05). 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were significantly elevated by LP addition, and they increased significantly as LP concentration increased in the formulation (P<0.05). Finally, consumer acceptance test indicated that the highest levels of LP incorporation (8%) had an adverse effect on general consumer preferences. In contrast, yanggaeng with moderate levels of LP (2%) is recommended based on its overall scores to take advantage of the antioxidant properties of LP without sacrificing consumer acceptability.