우리나라에서 흰등멸구는 매년 해외로부터 비래, 정착한 후 2~3세대를 경과하며 벼의 생육 및 품질 저하에 영향을 미치는 벼의 중요 해충 중 하나이며, 비래해충의 경우 비래 후 세대에 따른 변화 양상을 정확하게 예측하는 것은 방제시기와 방제수단을 결정하는데 중요하다. 공시충은 2018년 사육실에서(25±2 ℃, 60±5% RH, L:D=16:8) 누대사육하여 사용하였다. 흰등멸구가 국내에 비래 후 3세대까지 세대증식하면서 피해를 주는 것으로 가정하여 1세대와 3세대 간의 발육과 산란 등을 조사하였으며 얻어진 결과를 토대로 생명표를 작성하였다. 약충기간은 1세대와 3세대에서 각각 14.5일, 13.6일, 암컷 성충기간은 각각 17.2일, 11.8일로 나타났으며, 우화율은 98.3%, 85.0%로 조사되었 다. 산란기간은 각각 6.8일, 6.0일이었으며 산란수는 47.5마리, 122.6마리로 세대가 늘어나면서 증가하는 것으로 나타났다. 생명표 분석 결과, 순증가율, 내적자연증가율이 각각 5배, 1.7배 증가한 것으로 나타났다.

The leafhopper family Cicadellidae (Hemiptera: Auchenorrhyncha) is plant feeders, with mouthparts adapted for sucking. Almost of the them cause injuries of destruction to plants, including fruit trees and grain crops, and can be vectors of plant diseases. Therefor, Cicadellidae is one of the most important pests in the world. However, their identifications are very difficult because of small size and morphological similarities, and their exact biological information such as host plants, life cycle and distribution are not clear for most species. So, we provide and integrated biological database that present accurate diagnostic methods and ecological infromation.

The leafhopper subfamily Deltocephalinae are the biggest group belonging to the Cicadellidae. Deltocephalinae consists of 38 tribes, 923 genera, and 6,683 valid species in worldwide. The genus Scaphoideus Uhler of the subfamily is the largest genus of the tribe Scaphoideini with two subgenera and comprised about 160 species found in the world. Some of Scaphoideus species are serious pests and vectors of phytoplasma such as Scaphoideus titanus, S. luteolus. In this talk, the leafhopper genus Scaphoideus from Korea peninsula is reviewed with one new species and one new recorded species. Additionally, morphological information and descriptions of genitalia, and a key to the species of Scaphoideus are provided.

Photosynthetic characteristics and growth responses of Phalaenopsis Queen Beer ‘Mantefon’ orchid were determined in plants exposed to variable carbon dioxide (CO2) concentrations at 2-, 24-, and 36-weeks age (i.e., corresponding to juvenile, young, and mature vegetative growth stages, respectively). Plants were grown at 400 (control), 800, or 1,600 μmol･mol-1 CO2 for 6 hours during the nighttime for 32 weeks. Phalaenopsis ‘Mantefon’ in 2- and 24-week-old plants grown at 1,600 μmol･mol-1 CO2 had increased leaf number and net CO2 uptake compared with the plants grown at 400 μmol･mol-1 CO2. In 36-week-old of Phalaenopsis ‘Mantefon’, leaf number was significantly greater in plant grown at 800 and 1,600 μmol･mol-1 conditions compared with plants grown at 400 μmol･mol-1 CO2. Leaves that emerged after the start of the CO2 treatment were initially longer in the plants grown at 1,600 μmol･mol-1 CO2 than at 400 μmol·mol-1 C O2, but the final leaf length was shortest in the plants grown at 1,600 μmol･mol-1 CO2 condition. Plants showed crassulancean acid metabolism characteristic of nighttime CO2 uptake regardless plant growth stages. We found that growers may be able to promote leaf growth with increasing leaf number and reducing time to leaf initiation in the 36-week-old (i.e., mature stage) plants with 800 – 1,600 μmol·mol-1 CO2 and 2- and 24-week-old (i.e., juvenile and young stages) plants with 1,600 μmol·mol-1 C O2 for Phalaenopsis ‘Mantefon’.

The purpose of this study was to assess the changes in balance and proprioception of adults with limited ankle joint dorsiflexion, after the application of talocrural joint mobilization. The subjects of this study included 23 college students in their twenties with limited ankle joint dorsiflexion. The students were randomly assigned to the ankle joint mobilization group (AJMG, n=12) and the control group (CG, n=11). After 2 weeks of intervention using grade III talocrural joint mobilization in the anterior-posterior movement, the balance and proprioception of the subjects were assessed. Static/dynamic balance capabilities and ankle proprioception were analyzed using paired t-test and independent t-test. The dynamic balance and proprioception of AJMG were significantly improved after intervention (p<.05), In the comparison between the groups after the intervention, the dynamic balance and proprioceptive sense of AJMG were significantly improved compared to the control group (p<.05). This study suggests that AJMG can help improve the dynamic balance and proprioception.

This study aimed to analyze a high-performance liquid chromatography (HPLC) separation using a pentafluorophenyl column of parent drug hydroxychloroquine (HCQ) and its active metabolite, desethylhydroxchloroquine (DHCQ) applying to determine bioequivalence of two different formulations administered to patients. A rapid, simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for bioanalysis of HCQ and its metabolite DHCQ in human whole blood using deuterium derivative hydroxychloroquine-D4 as an internal standard (IS). A triple-quadrupole mass spectrometer was operated using electrospray ionization in multiple reaction monitoring (MRM) mode. Sample preparation involves a two-step precipitation of protein techniques. The removed protein blood samples were chromatographed on a pentafluorophenyl (PFP) column (50 mm × 4.6 mm, 2.6 μm) with a mobile phase (ammonium formate solution containing dilute formic acid) in an isocratic mode at a flow rate of 0.45 mL/min. The standard curves were found to be linear in the range of 2 – 500 ng/mL for HCQ; 2 – 2,000 ng/mL for DHCQ in spite of lacking a highly sensitive MS spectrometry system. Results of intra- and inter-day precision and accuracy were within acceptable limits. A run time of 2.2 min for HCQ and 2.03 min for DHCQ in blood sample facilitated the analysis of more than 300 human whole blood samples per day. Taken together, we concluded that the assay developed herein represents a highly qualified technology for the quantification of HCQ in human whole blood for a parallel design bioequivalence study in a healthy male.

The objectives of this study were to determine in vitro dry matter and energy utilization of hatchery waste products and to confirm whether in vivo energy digestibility of hatchery waste products could be estimated using in vitro data. Two in vitro assays were conducted for infertile eggs, unhatched eggs, culled chicks, and a mixture(20% dried infertile eggs, 20% dried unhatched eggs, and 60% dried culled chicks). In Exp.1, in vitro dry matter disappearance (IVDMD) of hatchery waste products was determined. In Exp.2, in vitro energy disappearance (IVED) was determined using undigested residues from Exp.1. The IVDMD of infertile eggs, unhatched eggs, culled chicks, and the mixture were 81.7, 88.7, 83.9, and 85.4%, respectively. The IVED of the test ingredients were 74.4, 85.1, 77.6, and 79.8%, respectively. Both IVDMD and IVED were greater in unhatched eggs compared with infertile eggs and culled chicks (p<0.05). In vivo energy digestibility was estimated well using prediction equations for hatchery waste products developed in the present study: In vivo energy digestibility(%) = 2.52 × IVDMD (%) – 133.95 with r2 = 0.70 and in vivo energy digestibility(%) = 1.63 × IVED(%) – 50.03 with r2 = 0.67. In conclusion, energy utilization of unhatched eggs was the greatest among test ingredients and energy utilization of hatchery waste products can be estimated using data from in vitro procedures.

Deltocephalinae (Hemiptera: Auchenorrhyncha: Cicadellidae) is the largest subfamily of Cicadellidae, and caused direct damage on their host plants by feeding and spawning, or as vectors of plant pathogens such as viruses, phytoplasmas, and bacteria. It contains majority of cicadellid vector species of economically important plant disease. Despite of economical pests, Deltocephalinae is difficult to identify due to morphological similarities and variations, yet, their biological information like host plants and taxonomical characters has not been constructed for accurate diagnosis of species. In this study, we build a database of integrated biological information such as host plants, distributional records and dorsal habitus of specimens, male genitalia, and DNA barcoding data with illustrations and photographs.

누에는 약제, 면직, 식용, 동물 사료 등에 활용도가 높아 동아시아에서 오랫동안 사육되어 왔다. 뽕잎을 먹고 자란 누에가 사료를 먹고 자란 누에보다 생장주기가 일정하고 더 건강하게 생장한다는 것은 잘 알려진 사실이다. 또한 최근의 연구에 따르면 동물 장내 미생물이 동물의 면역과 생장에 큰 역할을 한다고 알려졌다. 이러한 사실로부터 뽕잎에 존재하는 미지의 미생물이 누에 생장과 면역에 관여할 수 있다는 가설을 세우고 이를 확인하기 위한 연구를 수행하였다. 뽕잎 내생균을 분리하기 위해 뽕잎을 5mm 크기로 잘라 표면살균 후, 배지에 치상하여 자라나오는 세균을 분리하여 동정하였다. 흥미로운 점은 100개 이상의 뽕잎 절편으로부터 동일한 세균이 검출되었으며 계통학적 분석을 통해 동정한 결과, Pseudomonas syringae pv. syringae(Pss)로 확인되었다. 또한, 뽕잎을 섭식한 누에의 장 속과 분변에서 이 균의 존재를 확인하였다. 누에에 병을 일으키는 곤충병원성 곰팡이에 대한 Pss의 항진균 효과를 확인하기 위해 곤충병원성 곰팡이인 Beauveria bassiana, Cordyceps militaris와 식물병원성 곰팡이 Botrytis cinerea, Fusarium graminearum을 대상으로 대치배양을 통해 균사 생장 억제 정도를 확인하였다. 그 결과, 식물병원성 곰팡이의 생장 억제효과는 관찰되지 않았으나 곤충병원성 곰팡이의 생장이 50% 이상 감소되었다. 특히, 다량의 포자를 생성하여 곤충에 치명적인 병을 야기하는 B. bassiana의 균사 생장이 C. militaris에 비해 더 크게 감소하였을 뿐 아니라 Pss의 배양 추출물을 처리하였을 때 B. bassiana의 포자 발아가 지연되었다. 앞으로 누에 장 속에 안착한 Pss가 곤충병원성 곰팡이에 대한 면역력과 누에 생장에 미치는 영향을 확인해 볼 계획이며 이를 통해 건강하고 안정적인 누에 사육에 활용될 수 있는 미생물 제제를 만들고자 한다.

Japanese pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae) is considered as a serious pest in pine trees. To develop an eco-friendly strategy to manage this forest insect, we collected entomopathogenic fungi from Korean soil and assessed their virulence against the adults of the insect in laboratory conditions. As a result, two isolates with conidial suspension (1.0×107conidia/ml), showed 87% and 90% mortality 12 days after fungal treatment, respectively. We assessed the potential of the fungi-derived destruxin and protease as additives to the fungal isolates, and they showed insecticidal activity via feeding and spraying treatments. Finally, we produced fungal conidia in massive solid cultures and formulated wettable powders, and now studying optimal conditions of oil-based formulation with two isolates. Based on these results, we are evaluating the control efficacy of the fungal agents against M. alternatus in field conditions.

본 연구에서는 국내 유통 중인 농산물 9품목(n = 578)에 대한 납과 카드뮴 함량을 조사하고 이들의 섭취로 인한 위해성을 평가하고자 하였다. 납과 카드뮴의 함량은 마이크로웨이브 분해 후 ICP-MS로 분석하였다. 조사대상 농산물의 납 평균 함량은 각각 보리 0.014 mg/kg, 완두콩 0.010 mg/kg, 강낭콩 0.008 mg/kg, 녹두 0.006 mg/kg, 파인애플 0.008 mg/kg, 살구 0.016 mg/kg, 매실 0.015 mg/kg, 자두 0.021 mg/kg, 대추 0.019 mg/kg이었고, 카드뮴 평균함량은 보리 0.017 mg/kg, 완두콩 0.004 mg/kg, 강낭콩 0.007 mg/kg, 녹두 0.005 mg/kg, 파인애플 0.001 mg/kg, 살구 0.002 mg/kg, 매실 0.002 mg/kg, 자두 0.002 mg/kg, 대추 0.003 mg/kg이었다. 모든 시료의 납, 카드뮴 함량은 EU, CODEX 및 국내 기준보다 낮은 수준이었다. 조사 대상 농산물에 대한 납, 카드뮴의 인체노출량을 산출한 결과, 납은 잠정주간섭취허용량(PTWI, 25 μg/kg b.w./week)의 0.067%, 카드뮴은 월간잠정섭취허용량(PTMI, 25 μg/kg b.w./month)의 0.28%이었다. 이상의 결과는 조사 대상 농산물의 납, 카드뮴 오염도와 이들의 섭취에 의한 위해성이 모두 낮은 수준이라는 것을 보여준다.

This research explored antecedents of stickiness of m-commerce sites, based on the push–pull–mooring (PPM) framework, which was developed to explain what leads people to move from one original location to another. In the current study, the stickiness of m-commerce sites was used as the opposite concept to switching. Therefore, pull effects refer to the positive factors which make consumers stick to an m-commerce site, while push effects refer to the negative factors which make consumers not stick with an m-commerce site. After reviewing the literature, responsiveness, ubiquity, enjoyment, and effectiveness were selected as the pull effects, while privacy concern and efficiency were selected as the push effects. The amount of money spent in specific mobile sites was considered as a mooring effect. According to the results of the structural equation modeling analysis, efficiency actually had a negative impact on stickiness of m-commerce sites. Also, a test of the mooring effect suggested that the negative impact of efficiency on stickiness of m-commerce sites was greater for people who spent more money in their mobile shopping compared to those who spent less. For both the high and low spending groups, responsiveness was the important pull effect as it was the strongest predictor of enjoyment for the low spending group and the strongest predictor of effectiveness for the high spending group (note that enjoyment and effectiveness had the strongest positive impacts on stickiness of m-commerce sites for the low and high spending groups, respectively.). Therefore, the fast response and feedback on m-commerce sites should increase consumers’ stickiness to these sites regardless of the amount consumers spend on these sites. On the other hand, the duration and retention of the low spending group of consumers were influenced by enjoyment the most.

Ochratoxin A (OTA) is one of the most important mycotoxins owing to its widespread occurrence and toxicity including nephrotoxicity and potential carcinogenicity to humans. Since OTA is stable under most food processing conditions, OTA has been detected not only in a wide range of agricultural commodities such as cereal grains but also their processed products. Nonetheless, it is known that significant reduction of OTA may be achieved under higher temperature and alkaline conditions. In this study, the effects of retorting cooking process on the stability of OTA in spiked (20 μg/kg of dry weight basis) rice and oat porridge (10% solid content; w/v) in the presence and absence of baking soda was investigated using a laboratory horizontal steam retort system. The samples were heated in a pot at 85°C central temperature until it becomes gelatinized, packed in retort pouched, and heat-processed in pressurized retort machine (at 121°C for 25 min) followed by drying in 50°C oven overnight. Samples were analyzed for OTA by high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). The reduction of OTA in retorted rice and oat porridge were 54% and 17%, respectively, while greater reduction of OTA was observed at increased amount of baking soda. The reduction of OTA in retorted rice porridge with 0.5% and 1% baking soda were 55% and 66%, respectively. In the retorted oat porridge, reduction of OTA was also evident to result in 30% and 48% with 0.5% and 1.0% of added baking soda, respectively. These results suggest that OTA in rice and oat may be reduced significantly by retorting process. In addition, added baking soda may positively impact the reduction of OTA.