Pesticide application in agriculture provides significant benefits such as protection from disease, prevention of harmful insects, and increased crop yields. However, accurate toxicological tests and risk assessments are necessary because of many related adverse effects associated with pesticide use. In this review, we discuss and analyze residual pesticides contained in livestock feed in Korea. A pesticide residue tolerance standard for livestock feed has not been precisely established; so, risk assessments are required to ensure safety. Standards and approaches for animal criteria and appropriate methods for evaluating residual pesticides are discussed and analyzed based on technology related to animal product safety in Korea. The safety of livestock feed containing pesticides is assessed to establish maximum residue limits relative to pesticides. Analysis of residual pesticides in milk, muscle, brain, and fat was performed with a livestock residue test and safety evaluation of the detected pesticide was performed. Efficacy of organic solvent extraction and clean-up of feed was verified, and suitability of the instrument was examined to establish if they are effective, rapid, and safe. This review discussed extensively how pesticide residue tolerance in livestock feed and hazard evaluation may be applied in future studies.

Acute vascular rejection has been known as a main barrier occurring in a xenograted tissue of alpha 1,3-galactosyltransferase knock-out (GalT KO) pig into a non-human primate (NHP). Adenosine which is a final metabolite following sequential hydrolysis of nucleotide by ecto-nucleotidases such as CD39 and CD73, act as a regulator of coagulation, and inflammation. Thus xenotransplantation of CD39 and CD73 expressing pig under the GalT KO background could lead to enhanced survival of recipient NHP. We constructed a human CD39 and CD73 expression cassette designed for endothelial cell-specific expression using porcine Icam2 promoter (pIcam2-hCD39/hCD73). We performed isolation of endothelial cells (pAEC) from aorta of 4 week-old GalT KO and membrane cofactor protein expressing pig (GalT-MCP/-MCP). We were able to verify that isolated cells were endothelial-like cells using immunofluorescence staining analysis with von Willebrand factor antibody, which is well known as an endothelial maker, and tubal formation assay. To find optimal condition for efficient transfection into pAEC, we performed transfection with GFP expression vector using four programs of nucleofection, M-003, U-023, W-023 and Y-022. We were able find that the program W-023 was optimal for pAEC with regard to viability and transfection efficiency by flow cytometry and fluorescent microscopy analyses. Finally, we were able to obtain GalT-MCP/-MCP/CD39/CD73 pAEC expressing CD39 and CD73 at levels of 33.3% and 26.8%, respectively. We suggested that pACE isolated from GalT-MCP/-MCP pig might be provided as a basic resource to understand biochemical and molecular mechanisms of the rejections and as an alternative donor cells to generate GalT-MCP/-MCP/CD39/CD73 pig expressing CD39 and CD73 at endothelial cells.

The objectives of this study was to evaluate the degradability and digestibility of crude protein (CP), rumen undegradable protein (RUP), and individual amino acids (AA) on six by-product feedstuffs (BPF) (rice bran, RB; wheat bran, WB; corn gluten feed, CGF; tofu residue, TR; spent mushroom substrate from Pleurotus ostreatus, SMSP; brewers grain, BG) as ruminants feed. Three Hanwoo steers (40 months old, 520 ± 20.20 kg of body weight) fitted with a permanent rumen cannula and T-shaped duodenal cannula were used to examine of the BPF using in situ nylon bag and mobile bag technique. The bran CGF (19.2%) and food-processing residue BG (19.7%) had the highest CP contents than other feeds. The RUP value of bran RB (39.7%) and food-processing residues SMSP (81.1%) were higher than other feeds. The intestinal digestion of CP was higher in bran RB (44.2%) and food-processing residues BG (40.5%) than other feeds. In addition, intestinal digestion of Met was higher in bran RB (55.7%) and food-processing residues BG (44.0%) than other feeds. Overall, these results suggest that RB and BG might be useful as main raw ingredients in feed for ruminants. Our results can be used as baseline data for ruminant ration formulation.

In recent years, pavement distresses have been caused by diverse factors such as spalling, deterioration of repaired sections, blow-up, and alkali aggregate reaction due to changing climate environment of a concrete pavement and its construction and maintenance conditions (supply of materials, increase in use of de-icers, etc,). As a leading repair method for deteriorated concrete pavements, partial-depth repair is implemented in accordance with guidelines of material properties for joints of a concrete pavement and field application evaluation systems, but still some of the repaired sections become deteriorated again at an early stage due to poor construction quality and failure of response to environmental impacts. Distresses that can be corrected with partial-depth repairs are largely divided into those of repair materials and of the existing pavement bonded to repair materials, and combined distress of repair materials and the existing pavement. Although re-repair methods should be different by distress type and scale than conventional pavement repair methods, appropriate repair methods and guidance for re-repairs have not been in place so far, and therefore currently, re-repair practices follow the existing manual of partial depth repairs. Therefore, this study evaluated concrete bond characteristics by removing method and repair scope for an experimental section of frequently distressed pavements to determine a re-repair scope and method for deteriorated partial depth repair sections of concrete pavement, the number of which has increased over time.

참나무와 서어나무는 온대지역에 걸쳐 넓게 분포하고 있으며, 다양한 초식곤충의 먹이원이 되는 것으로 알려져 있다. 이 연구에서는 지리산국립공원 온대림에서 참나무림(반선)과 서어나무림(상선암)에서 초식곤충 활동량을 알고자 하였다. 각 조사지역에 0.1ha의 방형구를 설치하여 참나무와 서어나무의 분포를 확인 한 뒤 개체별로 초식곤충의 활동량인 잎 손상 지수를 측정하였다. 잎 손상 지수는 손상 퍼센트로 환산하여 분석하였다. 측정결과 전체 4,413장의 잎을 관찰하였으며, 참나무림에서는 2,683장, 서어나무림에서는 1,730장의 잎이 조사되었다. 초식곤충의 활동량은 참나무림과 서어나무림에서 각각 차이를 보였으며 서어나무림인 상선암에서 참나무의 식흔량과 서어나무의 식흔량 이 참나무림인 반선보다 모두 높게 나타났다. 이 연구결과를 바탕으로 온대림 우점 수종인 참나무와 서어나무에서 초식곤충의 활동량을 정량적으로 추정할 수 있을 것으로 생각된다.

감귤그린병(Citrus Huanglongbing Disease)은 전 세계적으로 감귤산업에 가장 큰 피해를 주는 병해로 국내 미분포종인 귤나무이(Diaphorina citri Kuwayama)가 매개충으로 알려져 있다. 바이러스에 감염된 나무는 5~12년내에 대부분 고사하며 열매는 크지 못하고 색이 들지 않는 증상을 보여 상품성을 저해시킨다. 현재 접목, 묘목, 매개충등의 유입으로 전 세계적으로 확산되고 있는 실정이다. 귤나무이의 국내 분포가능성이 꾸준히 제시되면서 유입 대비를 위한 예찰방법 및 동정법 개발이 필요한 실정이다. 제주도에는 총 33종이 분포하는 것으로 알려져 있으며(권, 1983; Cho & burckhardt, 2017) 2016년부터 지금까지의 조사로 11종의 나무이와 10종의 기주식물을 확인되었다. 귤나무가 속해있는 운향과를 기주로 하는 나무이는 발견되지 않았다.

Insect structural cuticular proteins (CPs) play a major role in determining the diverse physical properties of the cuticle as a result of interactions/cross-linking among themselves and with chitin. CP genes compose a large gene family and have been classified more than ten distinct families based on the presence of unique amino acid sequence motifs. In this study, we performed RNAi-based functional analysis of eleven genes (TcCPLCP1-11) in Tribolium castaneum, which belong to CPLCP (Cuticular Proteins of Low Complexity, Proline rich) cuticular protein family. RNAi for TcCPLCP7-11 caused lethal pupal-adult molting defects and/or abnormal cuticle morphology in the resulting adults. Ultrastructural defects of the cuticles from TcCPLCP7-11-deficient insects by TEM are also discussed.

Eggs from the mosquito genus Aedes exhibit high desiccation resistance that likely facilitate spreading some of them as a vector of human disease throughout the world. However, molecular mechanism underlying the embryonic resistant to desiccation has not well understood. In this study, we performed functional study of two ovary-specific yellow genes, AalY-g and AalY-g2, in the Asian tiger mosquito, Aedes albopictus. The eggs obtained from AalY-g or AalY-g2 RNAi females showed poor desiccation resistance. TEM analysis revealed that, unlike that seen in the dsEGFP-control eggs, no high electron-dense outer-endochorion was evident in the eggs from AalY-g- or AalY-g2-deficient females. These results suggest that both yellow genes at least play roles in the chorion formation, which appears to be critical for integrity and desiccation resistance in Ae. albopictus eggs.

In order to identify key nations and bird species of conservation concern we described multinational collaborations as defined using network analysis linked by birds that are found in all nations in the network. We used network analysis to assess the patterns in bird occurrence for 10,422 bird inventories from 244 countries and territories. Nations that are important in multinational collaborations for bird conservation were assessed using the centrality measures, closeness and betweenness centrality. Countries important for the multinational collaboration of bird conservation were examined based on their centrality measures, which included closeness and betweenness centralities. Comparatively, the co-occurrence network was divided into four groups that reveal different biogeographical structures. A group with higher closeness centrality included countries in southern Africa and had the potential to affect species in many other countries. Birds in countries in Asia, Australia and the South Pacific that are important to the cohesiveness of the global network had a higher score of betweenness centrality. Countries that had higher numbers of bird species and more extensively distributed bird species had higher centrality scores; in these countries, birds may act as excellent indicators of trends in the co-occurrence bird network. For effective bird conservation in the world, much stronger coordination among countries is required. Bird co-occurrence patterns can provide a suitable and powerful framework for understanding the complexity of co-occurrence patterns and consequences for multinational collaborations on bird conservation.

방울토마토의 수경재배 중 붕소+칼슘+규소 및 칼슘+규소의 복합 엽면시비가 수확 후 품질과 MAP 저장 중 저장성에 미치는 영향을 알아보고자 본 연구를 실시하였다. 엽면시비한 방울 토마토(‘Unicorn’)는 반숙 과상태에서 수확하여 산소투과성 필름으로 포장한 5oC, 11oC, 그리고 24oC에서 25일, 15일, 10일간 저장하였다. 붕소+칼슘+규소 복합처리한 방울토마토가 3가지 저장온도 모두에서 호흡과 에틸렌 발생이 억제되어 MAP 저장중 가장 낮은 생체중 감소와 가장 높은 외관상 품질을 보였다. 수확 후 조사한 방울토마토의 경도, 산도, 비타민 C 함량은 붕소+칼슘+규소 복합처리에서 가장 높았으며, 3가지 온도 모두에서 MAP 저장 후에도 모두 높게 유지되었다. 그러나 과피색, 라이코펜 함량과 당도는 수확 후에는 엽면시비 처리로 차이가 없었으나, 3가지 온도 모두 붕소+칼슘+규소 복합처리에서 가장 낮은 수치를 보였다. 이상의 결과로 볼 때 붕소+칼슘+규소 복합처리는 방울토마토의 수확후 생리 작용을 억제하고 경도, 산도, 비타민 C 함량을 높여 저장성을 향상시키는 것으로 판단되었다

Germinated brown rice flour has received much attention due to its health-functional ingredients such as dietary fibers. However, the food industry has faced challenges of using germinated brown rice flour in processed foods because it causes undesirable quality attributes such as high cooking loss and sticky texture. In this study, three different types of food additives (starches, hydrocolloids, emulsifiers) were incorporated into the formulation of extruded rice cakes and their combined effects on the cooking loss and textural properties of the germinated brown rice cakes were investigated. The addition of emulsifiers had a tendency to increase cooking loss and soften the noodle texture. Most of the starches except gelatinized and high-amylose rice flours showed similar effects to the emulsifiers. Xanthan gum, gelatin, and HPMC were positively effective in reducing the cooking loss of the rice cakes and increasing their hardness. When the ingredients were combined, the mixture of saemimyeon rice flour, HPMC, and xanthan/agar reduced the cooking loss by 66.7%. Most of all, this study provided an ingredient map that was plotted on the axis of hardness and cooking loss, depending on single and combined treatments of various ingredients.

Bryopsis africana and Pyropia kinositae are reported for the first time on the list of Korean marine algal flora based on integrated morphological-molecular study. Bryopsis africana from Korea is recognized with distinct main axes, robust thalli, pinnately and radially branched above, and basally denuded. Phylogenetic analyses revealed that B. africana was placed within a clade of Bryopsis. Bryopsis africana differs from B. corymbosa by 2.7% gene sequence divergence. Pyropia kinositae is characterized by oblong to lanceolate thalli, entire margin, purplish red in color, twice as long as broad cells in transversal section view. Phylogenetic analyses revealed that P. kinositae was placed within a clade of Pyropia. Pyropia kinositae differs from P. tenera by 1.5-1.8%, P. ishigecola by 1.2-1.3%, and P. yezoensis by 1.5-1.8% gene sequence divergence respectively.

Detailed morphological studies and molecular analyses based on plastid-encoded rbcL gene sequences were undertaken on Peyssonnelia species, a poorly known genus from Korea. We report new records for the Korean coast, Peyssonnelia harveyana and P. rumoiana. Peyssonnelia harveyana is chiefly characterized by P. rubra-type anatomy, closely packed perithallial filaments in firm matrix, hypothallial filaments arranged in parallel rows, thalli with appressed margins, hypobasal calcification, and unicellular rhizoids. Peyssonnelia rumoiana is principally characterized by two vegetative features, hypothallial filaments arranged in a polyflabellate layer, and perithallial filaments arising from the whole upper surface of each hypothallial cell (Peyssonnelia rubra-type anatomy). Our rbcL analyses revealed that P. harveynana and P. rumoiana were placed within a clade of Peyssonnelia. We also propose the new combination, Sonderophycus cauliferus comb. nov., for previous Peyssonnelia caulifera. Phylogenetic analyses revealed that our S. cauliferus was placed within a clade of Sonderophycus.

Three bamboo stands(Phyllostachys pubescens(Mazel) Ohwi, P. bambusoides Sieb. et Zucc, P. nigra var. henonis Stapf ex. Rendle) were selected to determine suitable biomass equations and productivity of Gajwa and Wola National Experimental Forests, Jinju, Southern Korea. Different independent variables such as diameter at breast height(DBH) or the combination of DBH and height(H) were used to develop biomass equations(Allometric model : logY = a + blog DBH; Linear-quadratic model : Y=aDBH + bDBH2; Linear model with DBH and height : Y=a + bDBH2·H) for each bamboo component from two age-sequence(current-year, > 1-year-old) of three bamboo stands. Based on statistical indicators, the most suitable equation model to estimate biomass from bamboo stands was a linear-quadratic model. Aboveground biomass of three bamboo stands estimated by the model was 48.864 Mg ha-1 for the P. pubescens, followed by 36.632 Mg ha-1 for the P. bambusoides, and 36.504 Mg ha-1 for the P. nigra var. henonis stands, respectively. The highest biomass in the P. pubescens stand was attributed to the morphological growth characteristics such as DBH and height. Belowground biomass was also highest for P. pubescens(53.35 Mg ha-1), followed by the P. bambusoides(36.73 Mg ha-1) and the P. nigra var. henonis(29.75 Mg ha-1) stands. The results indicate that the morphological growth characteristics such as DBH and height among bamboo species were the most important factor to determine bamboo biomass productivity at a local level.

This research was relative humidity (65, 75, 85 and 95%) growth characteristics of the oak mushroom (Lentinula edodes) 'Nongjingo’. At the relative humidity was 65% in growth chamber. On this condition the moisture content of medium surface was 60.5%. That was lower than other treatments. That condition makes Pileus was 77.1% and stipe was 65%. Among the growth characteristics according to the relative humidity, the pileus diameter at 75% relative humidity were 45.5mm that largest than other treatment and pileus thickness at 75% relative humidity were largest than other treatment. The stipe thickness at 65% relative humidity were 14.8mm that lagest than other treatment. The number of available stipes at 95% relative humidity were 13.3peace/2kg that largest than other treatment. The comparison yield ability of 1 cycle was 104.2g that result in largest than other treatment at 95% relative humidity. This result was associated to the relative humidity of fruiting bodies, the lower relative humidity result in the lower of yield. However, the lower relative humidity result in the higher number of whago, Especially, at the treatment of 65% relative humidity, all the fruit bodies were produced as Whago (dry). Therefore, the higher relative humidity influenced in the higher yield but produced water mushroom. That result was thought that ‘Nongjingo’ would be possible to produce high quality mushroom by adjusting the relative humidity appropriately.