게임 산업은 타 산업에 비해 짧은 시간에 여러 플랫폼 및 장르를 중심으로 고도의 성장을 하고 있다. 주요 게임 개 발사들은 미국과 유럽, 아시아에 위치하고 있으며 현재의 위치에 만족하지 않고 명실상부한 세계 게임 산업의 강자로 도약하기 위해서 노력을 하고 있다. 그 과정에서 최근 주목을 받고 있는 부분이 기능성게임이다. 기능성게임은 교육용, 스포츠, 헬스, 의료용 등을 중심 으로 빠르게 시장을 형성하고 있는 새로운 게임의 장르로서 그 성장 가능성은 매우 크다고 할 수 있다. 이러한 기능 성게임 시장에서 주도적인 역할을 하기 위해서는 현재와 같은 시장 형성기에 기술 경쟁력을 기반으로 선도적 위치를 선점하는 것이 매우 중요하고 이를 위해서 필요한 필수적인 요소 기술에 대한 R&D가 선행되어야 한다. 본 논문에서 는 기능성게임 분야별 인터뷰 및 설문 등의 조사 분석 방법을 통해 기능성게임의 분야별 R&D 핵심 기술을 제시하고 자 한다.

온라인 게임의 품질 관리는 게임의 완성도를 결정하는 중요한 단계 중 하나이다. 본 논문에서는 대학 수업을 통해 게임의 품질 관리를 효과적으로 운영하기 위한 포커스 그룹 테스트(Focus Group Test) 프로세스를 제안한다. 이를 위해 게임 테스팅 업체에서 시행하는 테스트 항목들을 검토하여 포커스 그룹 테스트를 통해 게임 품질 관리를 효과적으로 하기 위한 테스트 그룹과, 그룹별 세부 테스트 내용을 제안한다. 다음으로 대학 수업을 통해 포커스 그룹 테스트를 효 과적으로 운영하기 위한 운영 시스템을 제안한다. 마지막으로 개발 중인 상용 온라인 게임을 의뢰받아 포커스 그룹 테스트를 수행한 사례를 소개함으로서 제안한 내용이 효과적으로 운영될 수 있음을 검증한다. 제안한 방식의 수업 운 영을 통해 실무 지향적으로 수업이 가능하며 개발사와 대학 간의 효과적인 산학 협력이 가능할 것으로 판단된다

The purpose of this study was to determine the basic materials needed for nutrition and health programs for aged people. In order to set up these programs, the nutritional habit, nutritional knowledge and functional health status (mobility, functional assessment, and falls efficacy) of the aged were measured, and their relationships were determined. Data was collected on 57 aged individuals that participated in the 'Exercise Program for the Aged' conducted by the National Health Insurance Corporation. These 57 subjects were interviewed and tested by direct measurement prior to conducting the program. The data was analyzed using the SPSS 13.0 Package. According to the results, the nutritional habit ranged from 24-50 out of 50, and the average was 37.2, which was above the middle level. The average nutritional knowledge was of 4.02 out of 5, which was considered reasonably high. Especially, more than half of these subjects were uneducated, but they had a high interest in nutrition. The average mobility was determined to be 9.2 seconds and the falls efficacy was 64.2 out of 100. In addition, subjects answered that they had a fear of falling due to low confidence, which indicates that safety education for the prevention of falls should be provided to aged individuals in the future. The results showed that notable counter-relations between nutritional habit and functional health status existed.

Eukaryotic translation initiation factor 5A (eIF-5A) is essential for proliferation of eukaryotic cells, andwas identified as diagnotic marker in cervical intraepithelial neoplasia, cervical and endometrial cancer, but relatively little is known about thein vivo and in vitro expression patterns of eIF-5A in oral premalignant and malignant lesions mirror the expression levels observed in vitro with cells derived from normal oral mucosa, immortalized oral keratinocytes (IHOK) and primary and metastatic oral squamous cell carcinoma (OSCC). We used an oral squamous cell carcinoma (OSCC) progression model composed of cell lines and tissue specimens to characterize expression patterns by Western blotting and immunohistochemistry. eIF-5A and PCNA levels are elevated in IHOKand primary and metastatic OSCC cella as compatred to normal human oral keraitinocytes. eIF5A and PCNA expression was l imited to basal cells of normal oral mucosa. eIF-5A and PCNA expression is increased in dysplastic epithelium spreading to more superficial layers, and its expression levels correlated significantly with the degree of dysplasia. Well and moderately differentiated OSCC showed strong expression of eIF-5A and PCNA. These results suggest that upregulated expression of eIF-5A seems to be an important epigenetic alteration that accompanies oral carcinogenic progression, and eIF-5A could be used as an biomarker for oral premalignat lesion or squamous cell carcinoma

Considering the great potential of iron chelators at inhibiting the proliferation of tumor cells, in order to determine the molecular and biological basis for the effects of iron chelator in oral cancer, we investigated the effects of iron chelator, desferrioxamine (DFO), on the gene profiling analysis of immortalized human oral keratinocytes (IHOK), and oral cancer cells (HN12), using the cDNA microarray. We identified 46 clones cDNA exhibiting more than 2 fold overexpression in DFO treated IHOK and HN12 cells, and 94 cDNA reveal more than 2 fold down-regulated expression. Examination of gene expression that differs between DFO treated vs. control IHOK and HN12 cells apprear to be related to : cell cycle regulator, cell growth and apoptosis, signal transduction and stress. p21 for cell cell cycle factor was upregualted, and cyclin-cdk gene was decreased expression, so we observed cell cycle arrest in DFO treated IHOK and HN12 cells. In tumor growth, we have identified downregulation of hemidesmosomal protein (bullous pemphigoid antigen 1) and epiregulin expression in DFO treated IHOK and oral cancer cells. Signal transducers including mitogen-activated protein kinase-activated protein kinase 5, serine/thereonine kinase 6 were downregulated with DFO treated cells, suggesting the DFO regulates the p38 MAP kianse pathway in immortalized and maignant oral keratincytes. In conclusion, we have demonstrated the high-throughput utility of cDNA array hybridization in parallel to the gene expression analysis to identify genes that are expressed differentially in DFO treated with immortalized and malignant oral keratinocytes. The differentially expressed genes identified here should be informative in DFO-induced anti-cancer effects.

형질 전환 동물 생산에는 조직 및 시기 특이적 발현 조절이 가능하다는 장점 때문에 유즙 내로 외부 유전자를 발현시키는 시스템이 널리 이용되고 있다. 유전자 발현 즉, 단백질 생산은 프로모터의 강도뿐만 아니라 mRNA의 안정성에 의해서도 조절된다. 특히, polyadenylation에 의한 poly A의 길이는 in vivo와 올 in vitro에서 mRNA 안정성 및 목적 유전자의 번역효율에 영향을 준다. 본 연구에서는 이러한 mRNA 안정성이 목적 유전자의 발현에 미치는 영향을 알아보기 위해 3'-UTR 염기 서열을 분석하였다. 이 3'-untranslated region(UTR) 내의 poly A signal을 기준으로 putative cytoplasmic polyadenylation element(CPE) 부위와 downstream elements(DSE: U-rich, G-rich, GU-rich)의 염기 서열을 분석하고, 각각의 element를 기준으로 15 종의 luciferase reporter vector를 제작하여, 생쥐 유선 세포주(HC11)와 돼지 유선 세포주(PMGC)에 각각 transfection시킨 후 48시간 동안 배양하고 luciferase 발현량을 분석하였다. PMGC의 경우, luciferase의 발현은 exon 9의 CPE 2,3 및 DSE 1을 포함한 #6 construct에서 유의적으로 높은 발현량을 보였으며, exon 9의 CPE 2, 3과 DSE를 모두 포함하고 있는 #11 construct에서도 유의적으로 높은 발현량을 보였다. 이러한 결과는 형질 전환 돼지 생산에 있어 #6 및 11 construct의 사용은 목적의 유전자를 효과적으로 발현시키는데 기여할 것으로 사료된다.

  This paper develops a process monitoring system for real-time process control. The practical case is studied on a small and medium marine equipment company. For business process reengineering of the company, we adopt an approach based on information eng

This study examined the effect of growth temperature on the electrical and optical properties ofhydrogenated Al-doped zinc oxide (AZO:H) thin films deposited by rf magnetron sputtering using a ceramictarget (98wt.% ZnO, 2wt.% Al2O3). Various AZO films on glass were prepared by changing the substratetemperature from room temperature to 200oC. It was shown that intentionally incorporated hydrogen plays animportant role on the electrical properties of AZO:H films by increasing free carrier concentration. As a result,in the 2% H2 addition at the growth temperature of 150oC, resistivity of 3.21×10-4Ω·cm, mobility of 21.9cm2/V−s, electric charge carrier concentration of 9.35×1020cm-3 was obtained. The AZO:H films show a hexagonalwurtzite structure preferentially oriented in the (002) crystallographic direction.

  QFD(Quality Function Deployment) is a system for translating specific customer needs into detailed product requirements which are deployed throughout design, production, marketing and support operations. QFD is a team tool. However, most of commercial Q

Natto is a Japanese traditional food made from whole soybeans by the fermentation of Bacillus natto. The purpose of this study was to investigate the effect of Natto mucilage on lipid metabolism in rats. The levels of total cholesterol, LDL-cholesterol and triglyceride in serum of rats fed Natto mucilage diets were lower than those in control rats, but serum HDL-cholesterol and phospholipid were higher than those in control rats. The results indicate that Natto mucilage may have more beneficial roles in lipid metablism because it decreased serum cholesterol and increased bilirubin excretion.