From the course of screening of useful enzyme producing microorganism from insect guts, we isolated 9 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram negative bacteria grouped on Serratia sp., Pseudomonas sp., and Burkholderia sp.. Among them, an excellent lipase producing strain, Burkholderia sp. HY-10 identified by 16S rDNA analysis and biochemical methods, was further studied its lipase producing characteristics. It was isolated from a longcorm beetle, Prionus insularis and showed cell density dependent lipase producing activity in the culture media that contained olive oil as a carbon source. Maximum lipase production was achieved in the M9 media containing 0.5% yeast extract and 0.5% olive oil when cultured at 30℃ for 36-42 hrs.

  In mobile ad-hoc networks(MANETs), nodes are mobile and network topology changes very frequently. The mobility of nodes does affect the system performance. There has been considerable attention on the mobility models, however, most work is confined to t

Slotted ALOHA(S-ALOHA) is widely used in local wireless network. We analyze the performance of contention-based model in wireless LAN using S-ALOHA protocol. We analyze the performance of binary exponential backoff (BEB) algorithm under the slotted ALOHA

We consider an AS/RS(Automated Storage/ Retrieval System) which performs both of single and dual commands. The service times of single and dual commands are assumed to be differently distributed. Steady-state probabilities at the service completion epochs

In this paper, we model a two-server queueing system with priority, to which we put a restriction on the number of servers for each customer class. customers are divided into two different classes. Class 1 customers have non-preemptive priority over class

본 연구는 판소리 원격수업을 위한 콘텐츠를 <춘향가> 중 ‘자진 사랑가’를 활용하여 1∼6차시로 개발하고, 각 수업 내용을 연계한 논술과 가창 평가방안을 제시하였다. 1∼2차시는 영상과 이미지를 활용하여 조선 후기 역사적·문화적 배경을 바탕으로 판소리의 특징까지 이해할 수 있다. 3∼ 4차시에서는 ‘자진 사랑가’를 감상하고, 온라인 학습지로 사설붙임새를 학습하여 그 음악의 맛과 멋을 느낄 수 있다. 5∼6차시는 ‘자진 사랑가’의 가락과 음악적 특징을 가락선보로 나타내고 따라 부르며, 판소리 공연에서 소리꾼과 고수, 그리고 관객의 소통 방법을 체험할 수 있다. 이를 통해 판소리의 역사와 시대적·문화적 배경을 이해하며 감상할 뿐만 아니라, 판소리의 호흡, 창법, 표현 법 등을 익히고 그 음악적 특징까지 표현함으로써 학생들의 음악 표현 활동의 범위를 넓힐 수 있 다. 따라서 본 연구는 판소리의 음악적 특징을 이해하고 귀로 들은 가락을 가락선보로 시각화하는 활동과 함께 노래함으로써 이 음악의 아름다움과 가치를 깨닫고 애호하여 생활 속에서 활용하게 하는 데 그 목적이 있다. 앞으로 더 많은 원격수업용 콘텐츠의 개발이 필요한 시점에서 본 연구가 온라인 국악 수업을 위한 콘텐츠 개발의 한 예로 적용될 수 있길 바란다.

Seed dormancy is an important adaptive mechanism to protect seeds under the unfavorable environments. Unlike to wild type species, the seed dormancy trait of cultivated crops has been weakened by breeding programs during the domestication period. Weak seed dormancy often causes preharvest sprouting (PHS) problem in many cereal crops that result in significant economic loss. The seed dormancy is a quantitative trait loci (QTL) controlled by multiple genetic and environmental factors. So far, many QTLs for seed dormancy have been identified from rice and wheat as well as in the model plant Arabidopsis. Unveiling of QTL genes and complex mechanisms underlying seed dormancy is accelerated by the rapid progress of crop genomics. In the present study, we reviewed current status of research progress on the seed dormancy QTLs and correlated genes in Arabidopsis and cereal crops.

본 연구는 대상자의 수가 적고 대조군이 없어 연구결과를 일반화시키기에 제한점이 있으나, 연구결과에서 원예치료 프로그램이 청소년의 자아 존중감과 학교생활적응에 미치는 영향을 검증한 결과 통계적으로 유의한 차이가 나타났다. 이러한 원예치료 프로그램을 대상자와 함께 하는 동안 그들의 변화하는 모습에서 프로그램 실시 전에 비해 프로그램 실시 후 청소년들의 자아존중감과 학교생활적응에 원예치료가 많은 영향력을 미친다는 사실을 알 수 있었다. 그리고 다양한 식물과 재료를 사용하여 새로운 것에 대하여 경험해보게 하고 새로운 방법을 시도해 볼 수 있도록 하여 청소년들의 건강한 성장과 발달을 위하여 다양한 원예치료 프로그램 개발이 필요하다고 사료된다.

This study was conducted to investigate the effects of Lycii fructus and Astragalus membranaceus mixed extracts on immunomodulators and prevention in a streptozotocin-induced diabetes rat model. A total of 28 male rats were divided into four dietary groups and fed a commercial diet (A), commercial diet plus induced diabetes by a streptozotocin (STZ) injection (B), induced diabetes by STZ plus medicinal crop extracts(I&H®) diet (C), and medicinal crop extracts (I&H®) diet (D). Immunoblotting analyses revealed cytokine expression, and ELISA analyses revealed immunoglobulin E and nitric oxide production. As a results, the tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) as a inflammatory cytokine were decreased. Interleukin-6 (IL-6) and signal transducer and activation of transcription 3 (STAT3) cytokine related in diabetes expression through JAK/STAT3 pathway were also decreased. Furthermore, immunoglobulin E and nitric oxide production were decreased in the serum and lens, respectively. These results suggest that Lycii fructus and Astragalus membranaceus mixed extracts provide positive effects on immunomodulators and prevention in diabetes and eye disease complications.

The purpose of this study evaluated the immunoregulatory effect of phellinus linteus ethanol (PLE) extracts on liver damage on carbon tetrachloride (CCl4) induced in rats. Four-week old Male Sprague-Dawley rats were divided into the three experimental groups randomly; Control group, CCl4 group, CCl4 + PLE group. We found that effect of PLE on IFN-γ, STAT1 and pSTAT1 was decrease in vivo. Several genes were demonstrated to be IL-4 inducible prior to the discovery of STAT6. IL-4, STAT6 and pSTAT6 decreased significantly lower in CCl4 + PLE than the CCl4 group. Our data indicated that cytokine protein production were increased in CCl4 group with CCl4 + PLE group. In our data indicate that IgA levels in MLN lymphocytes were low, while IgE was high in CCl4 + PLE group compared with CCl4 group. Therefore, the results of this study show that PLE can be proposed to protect the liver against CCl4-induced immunoregulatory activity in rats.