Climate change has led to increased insect pests and pest distribution changes. Traditionally, chemical control using synthetic pesticides has been the main method for pest management, but the emergence of pesticide-resistant pests has become a problem. There is a need to develop new pest control agents to overcome these issues. Entomopathogenic fungi used in pest management have minimal environmental side effects and possess a mechanism of action distinct from that of synthetic pesticides. However, there is a need for the development of technologies to maximize the insecticidal effects of fungi against pests, and expressing and releasing dsRNA within the fungi can preemptively knock out the activation of the insect’s defense system, thereby enhancing the insecticidal effect. Controlling insect defense genes and using entomopathogenic fungi as bio-carriers forms a new pest management strategy. This approach, described as a “microbial insecticide agents development strategy of cassette concept, ” can versatilely modify genes and microbes. It is expected to overcome the limitations of synthetic pesticides.

Since rice is the main food in Korea, there are no regulations on corn milling yet. Corn is known as one of the world's top three food crops along with wheat and rice, and it is known that 3.5 billion people worldwide use corn for food. In addition, corn mills are not developed or sold in Korea, but the use of corn mills is increasing significantly in many countries in Southeast Asia. In the Philippines, as Korea's rice mill import increases, Korea's KAMICO (Korea Agricultural Machinery Industry Cooperative) and domestic company A agreed to develop a corn mill jointly with PHilMech, an organization affiliated with the Philippine Ministry of Agriculture. However, research on corn milling was very insignificant, so the development was carried out based on the technology of Korea's rice mill. Rice milling is performed by peeling off the skin of rice and producing brown or white rice, so it is carried out by removing the skin and cutting the skin. On the other hand, in the corn mill, the skin of the corn is peeled, pulverized and selected to produce main products suitable for edible use. Therefore, in order to develop a corn mill, processes such as peeling, transfer, grinding, sorting, and by-product separation are required, and suitable parts must be developed. In addition, the performance must be gradually improved through experiments in which corn is repeatedly milled. The Philippines produces 7.98 million tons/year of corn, which is about 100 times that of Korea, and is mostly consumed as a staple food. This is about 10% of the total crop production in the Philippines. In addition, the main cultivation complexes of corn are the mountainous regions of Tarlac or Pangasinan, and the produced corn is 72.4% of the so-called yellow corn called Arabel and Sarangani, and the remaining 27.6% are known as white corn. In this study, it was intended to produce grains of 2.5 mm or less suitable for food for yellow corn and to develop a corn mill for 200 kg per hour. Detailed conditions for development are stipulated as more than 55% of the main product recovery rate, more than 31% of the by-product recovery rate, less than 5% of the raw material loss rate, and more than 80% of the embryo dislocation rate. In this study, to achieve this, the overall process of the corn mill was developed, and the optimal conditions for the corn mill were obtained through the development of parts and empirical tests to improve performance. In addition, it was intended to achieve the development goal by evaluating and analyzing the performance of each part so that it did not conflict.

Safeguards systems and measures are determined through diversion scenario analysis based on the facility design information submitted to the IAEA when a new nuclear facility is introduced. While the concept of safeguards-by-design (SBD), which considers the safeguards from the design phase for a facility operator to minimize unplanned changes or disruption to facility operations as well as for the IAEA to increase the efficiency and effectiveness in safeguards implementation, has been emphasized for more than a decade, there is no practical tool or guidance on how to apply it. In this study, we develop a diversion path analysis tool and introduce how to apply SBD using it. A diversion path analysis tool was developed based on the elements that constitute diversion and the algorithm generated based on the initial information of facility and nuclear material flow. The results of utilizing the analysis tool depending on a different level of facility information and the safeguards set-ups were compared through examples. Taking a typical light water reactor as an example, the test analyzed the automatic generation of dedicated routes, configuration of safeguards measures, and diversion path analysis. Through this, the application and limitations of the analysis tool are discussed, and ideas for utilization according to the SBD concept and necessary regulatory guidance are proposed. The results of this study are expected to be directly utilized to domestic nuclear control during the regulation process for a construction of new nuclear power systems, and furthermore, to enhance national credibility in the engagement with the IAEA for implementation of safeguards.

To evaluate the safeguards system or performance in a facility, it is crucial to analyze the diversion path for nuclear materials. However, diversion paths can range from the extremely simplified to the complicated depending on the level of knowledge and the specific person conducting the analysis. This study developed the diversion path analysis tools using an event tree and fault tree method to generating diversion paths systematically. The essential components of the diversion path were reviewed, and a logical flow was developed for systematically creating the diversion path. An algorithm was created based on the facility design components and logical flow, as well as the initial information of the nuclear materials and material flows. The event tree and fault tree analysis tools were used to test the path generation algorithm. The usage and limitations of these two logic methods are discussed, and ideas to incorporate the logic algorithm into practical program tools are suggested. The tests were analyzed on a typical light water reactor as an example, including automatic generation of dedicated pathways, configuration of safeguards measures, and analyzing paths with strategies for avoiding safeguard systems. The results led to the development of a draft pathway analyzer program that can be applied to general nuclear systems. The results of this study will be used to develop a program module that can systematically generate diversion paths using the event tree and fault tree method. It can help to guide and provide practical tools for implementing SBD.

실버 페이스트는 상대적으로 낮은 열처리로 공정이 가능하기 때문에 전자 소자 응용분야에서 유용한 전극 재료이다. 본 연구에서는 은 페이스트 전극에 대기압 플라즈마 제트를 이용하여 전극 표면을 처리 했다. 이 플라즈마 제트는 11.5 kHz 작동 주파수에서 5.5 ~ 6.5 kV의 고전압을 사용하여 아르곤 분 위기에서 생성되었다. 플라즈마 제트는 대기압에서 수행함으로써 인쇄 공정에 더 유용할 수 있다. 플라즈 마 처리시간, 인가된 전압, 가스유량에 따라 전극의 표면은 빠르게 친수성화 되었으며 접촉각의 변화가 관 찰되었다. 또한, 대면적 샘플에서 플라즈마 처리 후 접촉각의 편차가 없었는데, 이는 기판의 크기에 관계없 이 균일한 결과를 얻을 수 있었다는 것을 의미한다. 본 연구의 결과는 대면적 전자소자의 제조 및 향후 응 용 분야에서 적층 구조를 형성하는데 매우 유용할 것으로 기대된다.

Even though it is emphasized to apply safeguards-by-design (SBD) concept in the early phase of the design of a new nuclear facilities, there is no clear guideline or tools for the practical SBD implementation. Generally known approach is trying to review whether there is any conflicts or shortcomings on a conceptual safeguards components in a design information. This study tries to build a systematic tools which can be easily applied to safeguards analysis. In evaluating the safeguards system or performance in a facility, it is essential to analyze the diversion path for nuclear materials. Diversion paths, however, can be either extremely simplified or complicated depending on the level of knowledge and purpose of specific person who do analyze in the field. In the context, this study discusses the applicability of an event tree and fault tree method to generating diversion paths systematically. The essential components constituting the diversion path were reviewed and the logical flow for systematically creating the diversion path was developed. The path generation algorithm based on the facility design components and logical flow as well as the initial information of the nuclear materials and material flows was test using event tree and fault tree analysis tools. The usage and limitation of the applicability of this two logic methods are discussed and idea to incorporate the logic algorithm into the practical program tools is suggested.The results will be used to develop a program module which can systematically generate diversion paths using the event tree and fault tree method.

In order to effectively and efficiently apply safeguards to new nuclear facilities, it is recommended to apply safeguards-by-design concept. In evaluating the safeguards in the early stage of the design of a facility, it is essential to analyze the diversion path for nuclear materials. This study suggests a simple method which can generate diversion paths. The essential components constituting the diversion path were reviewed and the logical flow for systematically creating the diversion path was developed. The path generation algorithm is based on this components and logical flow as well as the initial information of the nuclear materials and material flows in a planned facilities. The results will be used to develop a program module which can systematically generate diversion paths using the event tree and fault tree method.

Nine fish and one clam species were collected from freshwater environments in Korea, including four lakes, two streams, and the Nakdong River, to investigate the host-associated bacteria. Hundreds of bacterial strains were isolated from the samples using a cell sorter and a dilution plating method. After identification of the bacterial strains using 16S rRNA gene sequences, 42 strains with greater than 98.7% sequence similarity with validly published species were determined to be unrecorded bacterial species in Korea. These strains were phylogenetically diverse and assigned to four phyla, six classes, 17 orders, 27 families, and 32 genera. At the genus level, the unrecorded species were classified as Corynebacterium, Mycobacterium, Mycolicibacterium, Gordonia, Williamsia, Modestobacter, Brachybacterium, Sanquibacter, Arthrobacter, and Mycolicibacterium of the class Actinobacteria; Empedobacter, and Flavobacterium of the class Flavobacteriia; Fictibacillus, Psychrobacillus, Cohnella, Paenibacillus, Rummeliibacillus, Enterococcus, and Vagococcus of the class Bacilli; Aquamicrobium, Paracoccus, and Sphingomonas of the class Alphaproteobacteria; Achromobacter, Delftia, and Deefgea of the class Betaproteobacteria; and Aeromonas, Providencia, Yersinia, Marinomonas, Acinetobacter, and Pseudomonas of the class Gammaproteobacteria. The 42 unrecorded species were subjected to further taxonomic characterization using gram staining, cellular and colony morphological determination, biochemical analyses, and phylogenetic analyses. This paper provides detailed descriptions of the 42 previously unrecorded bacterial species.

Royal jelly (RJ) is a gelatinous substance that bees produce to feed bees and queen bees. It’s frequently sold as a dietary supplement to treat a variety of physical ailments and chronic diseases. While it has long been used in traditional medicine, its applications in Western medicine remain controversial. The inhibitory effect of royal jelly on osteoarthritis was investigated in primary cultured rat cartilage cells and monosodium-iodoacetate (MIA)-induced arthritis rat model 10-hydroxy-2-decenoic acid (10-HAD) is the main fatty acid present in RJ. Among the criteria for RJ quality analysis, 10-HAD content has been proposed as a freshness parameter. We investigated the effect of RJ on the improvement of osteoarthritis on SD rats and they were divided into five groups. In this study, we examined the effect of enzymatic royal jelly (ERJ) administration on osteoarthritis. To determine the antiinflammatory effects of RJ, tumor necrosis factor alpha (TNF-α) and Interleukin-6 (IL-6) expression were measured after lipopolysaccharide (LPS) activation in RAW 264.7 cells. In in vivo animal study, osteoarthritis was induced by intra-articular injection of MIA into knee joints of rats. As a results, ERJ showed that TNF-α and IL-6 levels were decreased by ERJ treatment in a dosedependent manner. In conclusion, ERJ extract was able to inhibit articular cartilage degeneration by preventing extracellular matrix degradation and cartilage cell damage. It was considered that ERJ extract may be a potential therapeutic treatment for degenerative osteoarthritis.

최근 봄철 기온이 높아짐에 따라 마늘 뿌리응애 피해가 늘어나고 있는 실정이다. 이러한 뿌리응애 피해를 최소하 하고자 발생양상과 22종의 약제에 대하여 살비효과를 조사하였다. 마늘 재배시기 중 뿌리응애의 발생은 충북 보은 등 5지역에서 2015년부터 2017년까지 3년간 3월부터 6월까지 뿌리응애 발생밀도를 조사하여 발생양상을 확인하였다. 뿌리응애는 2016년에 가장 많이 발생하였으며, 발생시기는 월동 후 3월 상순부터 발생하여 6월 중순경까지 지속적으로 밀도가 높아지는 양상을 나타내었다. 조사된 5지역 중 가장 높은 밀도로 조사된 보은지역은 6월 상순경 주당 13.3마리가 발생하였다. 뿌리응애에 대하여 우수한 살비효과를 나타낸 약제는 분무처리시 약제처리 10일 후에서 dimethoate, azocyclotin, abamectin, hexythiazox, cabosulfan, spirotetramat, benfuracarb가 90% 이상의 살비효과를 나타내었다