Although aronia (Aronia melanocarpa (Michx.) Elliott) contains higher levels of polyphenols and more antioxidant activity than other berries, it is a berry that is difficult to eat raw due to its strong astringent taste and lack of sweetness. Therefore, in this study, we investigated the effect of tannin reduction of aronia by bioconversion method using mushroom mycelia cultures. Aronia and liquid cultures of Lentinula edodes and Phellinus linteus mycelia were mixed and then treated for 48 hours at 60°C. Tannin content, total polyphenol, total flavonoid and antioxidant activities (DPPH, ABTS radical-scavenging activities and FRAP activities) were investigated. The tannin content decreased from 64.2 mg ECE/g to 57.9 mg ECE/g (9.8% reduction) when treated with liquid culture of L. edodes and from 77.3 mg ECE/g to 47.9 mg ECE/g (38.1% reduction) from treatment with a liquid culture of P. linteus. Therefore treatment with mushroom mycelia culture solution may improve the palatability of aronia reducing the astringent taste.

This study investigated the quality characteristics of black soybean sediments to diversify the availability of soybean. The cooking method selected for black soybean sediment preparation was a pressure cooking process without soaking, considering the isoflavone content. The black soybean sediments were prepared by the addition of 0, 10, 30, 50 and 100% (w/w) black soybean. When 0% to 100% black soybean was added to the black soybean sediments, the moisture and crude protein contents increased from 53.17% to 54.41% and from 12.07% to 21.68%, respectively. The total isoflavone content of the black soybean sediments was increased from 2.69 μg/g to 696.09 μg/g, respectively, by the addition of black soybean. The anthocyanin content of the black soybean sediments ranged from 279.29 μg/g to 387.8 μg/g by the addition of black soybean. The total polyphenol content and the total flavonoid content of the black soybean sediments range from 1.72 mg/g to 2.00 mg/g and 0.89 mg/g to 0.92 mg/g, respectively, by the addition of black soybean. Given the isoflavones, total polyphenol, and anthocyanin content of the black soybean sediments, it is appropriate that the ratio of added black soybeans is at least 50% after the pressure-cooking process, regardless of soaking.

The purpose of this study was to investigate the quality characteristics and antioxidant activity by collecting four times on six samples of Jeonbuk regional soybean pastes (Doenjang) traditionally prepared for this study. The water content of samples decreased according to progress of the aging period and the water content of the fourth sample collected was 42.40~59.64% (p<0.05). The salinity of the fourth Doenjang samples was 11.80~18.60%. The amino-type nitrogen content was 122.67~540.33 mg% immediately after the preparation of Doenjang in the Jeonbuk region and the content of the fourth collection samples increased from 251.49 to 982.36 mg% (p<0.05). The isoflavone glycosides decreased but daidzein, genistein and glycitein, which are aglycones, increased during aging periods. The total polyphenol content of the fourth collected samples was 11.99~19.27 mg GAE/g (p<0.05). The DPPH radical scavenging activity, ABTS radical scavenging activity and FARP of the fourth Doenjang samples were 51.88~81.21%, 84.14~90.32%, and 1.08~3.11 mg Trolox/g, respectively. As a result of quality analysis on Doenjang, the superiority of traditional Doenjang has been proven by the increase of amino nitrogen content and antioxidant activity according to the aging period. However, factor analysis on quality differences of regional Doenjang should be conducted to ensure standardization and quality improvement.

최근 대추 재배 면적이 5,160ha로 전국적으로 면적이 계속 증가하고 있는 추세이다. 대추의 재배면적이 증가하면서 기존에 대추에 발생하지 않던 다양한 해충들 또한 발생을 하고 있는데 장님노린재의 경우 2015년 대추에 피해를 준다는 것이 확인 되었다. 현재 대추에 발생하는 장님노린재류는 두 종으로 애무늬고리장님노린재와 초록장님노린재이다. 애무늬고리장님노린재와 초록장님노린재는 대추 발아기부터 새순을 흡즙하여 피해를 주는데 피해를 받은 새순과 잎은 정상적으로 생육하지 못하고 잎에 구멍이 뚫리는 등 비정상적인 생육을 하게 된다. 장님노린재의 경우 거의 모든 대추과원에서 발생을 하고 있으며, 현재까지 대추과원에서 정확한 발생 기간과 방제 약제에 대한 연구가 이루어지지 않았다. 따라서 본 연구에서 2017~2018년에 걸쳐 2년 동안 발생 소장을 조사 하였으며, 시판되고 있는 농약 10종을 대상으로 약제 선발 시험을 수행하였다. 그 결과 시기별 밀도 조사의 경우 약충은 5월 상순부터 발생을 시작하여 7월 중하순까지 발생을 하였으며, 성충은 5월 하순부터 발생을 시작하여 7월 중하순까지 발생을 하였다. 또한 2017년의 경우 약충은 5월부터 꾸준히 발생량이 증가하다 7월 상순 많은 강우로 인해 밀도가 크게 감소하였으며, 2018년의 경우 5월과 7월에 발생량이 많고 6월에 발생량이 급감하여 마치 2세대가 나타나는 듯한 현상을 보였다. 물론 이는 더욱더 시간을 두고 지속적인 관찰이 필요할 것으로 판단된다. 약제 선발 시험의 경우 10종의 약제 중 페니트로티온 등 6종의 약제에서 방제가가 80% 이상인 것으로 나타났다. 따라서 대추에 발생하고 있는 장님 노린재의 피해를 줄이기 위해서는 장님노린재가 발생하기 시작하는 5월부터 방제를 시작하여 초기밀도를 낮추어야하며, 이동성이 좋은 장님노린재의 성충이 외부로부터 유입이 될 수 있으므로 성충이 발생하는 7월까지는 꾸준한 관찰을 해 주어야만 그 피해를 줄 일 수 있을 것으로 판단된다.

The purpose of present study was to investigate the effect of kinesio taping on pulmonary function in stroke patients using manual wheelchairs. Twenty stroke patients were divided into a wood chair group (WCG, n=10), a wheelchairs group (WG, n=10), and a kinesio taping with wheelchairs group (KWG, n=10). Taping with wheelchairs group was applied kinesio taping on back muscles (vertical paraspinal strips and oblique strips). All three groups were trained in upright seated posture for 30 minutes. Pulmonary function tests were performed with forced expiratory volume in one second, forced vital capacity and peak expiratory flow as spirometer. There was a significant increase in forced expiratory volume in one second and forced vital capacity only in KWG. The results of this study demonstrate that kinesio taping has an immediate effect on the improvement of pulmonary function in stroke patients using manual wheelchairs.

This study aimed to determine the effect of McKenzie lumbar support on pulmonary function in Stroke patients. Twenty subjects (n=20) were divided into two groups: a McKenzie lumbar support group (MLS group=10), a control group (n=10). Pulmonary function was performed to assess its effectiveness. A spirometer was used to measure the forced vital capacity (FVC), forced expiratory volume in one second (FEV1), peak expiratory flow (PEF). The intervention was conducted for four weeks. In the MLS group, FEV1, FVC, and PEF were increased after McKenzie lumbar support. (p<0.05), while no significant differences in the variables were found in the control group (p>0.05). There were no significant differences in variables between the MLS group and the control group (p>0.05). Our findings suggest that applying Mckenzie lumbar support may be an alternative maneuver to improve pulmonary function in stroke patients.

Currently, Carposina sasakii (peach fruit moth) is considered economically important pest in organic jujube orchards. However, until now, research has not been conducted to control C. sasakii. The control period is very important for moth larvae to contact organic materials. Therefore, this study were conducted to determine when control using organic materials selected in 2017. In the method, parafin oil selected in 2017 was treated seven times at interval of seven days, and each treatment interval was seven days until 7th treatment. As a result, when the control started at the early to mid July, the control values were about 75%, and when the control started at late July to mid August, the control values were over 85%. However, when the control started after mid August, the control value was less than 30%. Therefore, in order to effectively control C. sasakii, control must be started by early August.

Gastrodia elata Blume often has been used for the treatment of headaches, convulsions, hypertension, and neurodegenerative diseases. The main active constituents are gastrodin, 4-hydroxybenzyl alcohol, vanillyl alcohol, 4-hydroxybenzylaldehyde and parishin A, B, C and E. Because Gastrodia elata has also unacceptable off-odor (swine barnyard-like) for food, there is a need to reduce it as well as allow for greater utilization as a functional food materials. In this study, a major off-odor producing substance of Gastrodia elata was fractionated by steam distillation and silica gel column chromatography. The substance was identified as p-cresol(4-methyl phenol) by GC-MS analysis and comparison of the retention time with that of an authentic compound in GC. The content of p-cresol in fermented Gastrodia elata was decreased. A fermented sample of Latobacillus sakei for 2 days was reduced to 54.7%, when compared with a unfermented sample. The five parishin derivatives in Gastrodia elata were identified by HPLC-MS analyses, and a comparison of HPLC retention times with those of authentic compounds. When compared with parishin derivatives of an unfermented Gastrodia elata, those of Gastrodia elata fermented by L. sakei, increased to 18.3% for 2 days. Increases of about 14.0~38.4% of the total phenolic compounds and 57.4~77.3% total flavonoids were found in fermented extracts, by 3 lactic acid bacteria strains. They were compared with 97.1±2.9 μg/g and 40.9±2.0 μg/g in the unfermented control, respectively. The extracts of Gastrodia elata Blume that were fermented by lactic acid bacteria had higher DPPH free radical scavenging activity and FRAP reducing power than the unfermented control.

The purpose of the study was to determine the effect of joint mobilization with respiratory muscle taping on pulmonary function and chest expansion ability in patients with chronic stroke. Thirty subjects (n=30) were randomly divided into three groups: a joint mobilization group (n=10), a respiratory muscle-taping group (n=10), and a joint mobilization with respiratory muscle-taping group (n=10). Measurements for pulmonary function and chest expansion were performed to assess its effectiveness. A spirometer was used to measure the pulmonary function, and a tape measure was used to assess the chest expansion. In the joint mobilization group, peak expiratory flow (PEF) was increased. Forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and upper and lower chest expansion were also increased. All of variables of respiratory muscle-taping group also were increased. FEV1 and FVC were significantly different between joint mobilization with respiratory muscle-taping and respiratory muscle-taping group. The results of the present study suggest that mobilization with respiratory muscle taping increase the pulmonary function and chest expansion.

We investigated the distribution of ixodid ticks in severe fever with thrombocytopenia syndrome (SFTS) case reported areas using flagging method from May to November 2014 in Korea. We collected 13,471 ixodid ticks with 2 genera and 3 species. Among them, Genus Haemaphysalis and Ixodes accounted for 99.9% (13,455) and 0.1% (16), respectively. Genus Haemaphysalis consisted of 2 species, H. longicornis (96.2%) and H. flava (3.7%). I. nipponensis was only collected in Genus Ixodes. H. longicornis was the dominant species (over 90%) in most study areas. Ixodid ticks were evenly distributed at various environmental sites, such as residences, hills, grassfields, fields, graves, copses, other sites. The further study on SFTSV infection in ixodid ticks will be needed.

A surveillance of chigger mites was performed to monitor the incidence of scrub typhus vectors at 4 environmental collection points of 6 locations from September to November 2014 in Korea. During the survey period, 420 chigger mites were collected and the dominant species was Leptotrombidium scutellare (42.6%). The first appearance of chigger mite was at 37th week (9.3.-9.10.) and the collected numbers of chigger mites was the highest at 43rd week (10.17.-10.23.). In Goryeong-gun, 299 chigger mites were collected, whereas 5 chigger mites were collected In Yesan-gun. The high environmental collecting rates were recorded at rice field (56%) and waterway (20%). The annually collected numbers (2012-2014) of chigger mites were compared with the average temperatures in August. This result suggests that the average temperature in August might be related with the annual incidence of scrub typhus vectors in Korea. However, the relationship between climate factors and the density of chigger mites needs to be studied by long-term periodical surveillance.

Immuno-MemBlot is a technique for detecting, analyzing, and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular or slot templates directly onto the membrane. Recently we developed a new Immuno-MemBlot (IMB) method applying immunoreactions and coloring procedures directly in the wells of MemBlot apparatus, which were connected by canals to perform drainage for reagent application and buffer irrigation. This IMB method was designed to get theimmunoblot results more rapidly and clearly than the previous immunoblot ones. This study is aimed to evaluate the analytical accuracy of IMB using different biological assay. In the sensitivity test of IMB the monoclonal antibody can clearly detect the 30 ng (about 12 pM) of Mucocidin peptide (35 mer), and is also available to detect at least 10 ng (about 4 pM) of Mucocidin peptide (35 mer). The IMB was effective in the quantitative analysis of methothrexate (MTX) assay for cellular apoptosis. And more, this IMB is useful to screen large number of specific samples with ease and accuracy in a short time. In the screenings for the presence of Mucocidin in saliva the quantitative comparison is conspicuous among 48 persons depend on the different conditions ofgender, drinking and smoking habits, and oral diseases. Therefore, it is presumed that, even though the target proteins were partly degraded, a specific epitope can be detected if a monoclonal antibody was still reactive. Conclusively, these data suggest that the IMB can be useful in the primary qualitativeand quantitative analysis of proteins in various fluids, i.e., blood, saliva, tear, urine, etc.

본 연구는 3,7-diarylpyrazolo [1,5-α]pyrimidines의 효과적인 one-pot regioselective 합성을 보여준다. 더욱이, 그 유도체는 뛰어난 CB1R 저해 활성을 나타냈다. 3,7-position에 diaryl group이 치환된 pyrazolo [1,5-α]pyrimidine은 CB1R 후보로서 가능성 있는 pharmacophore이다.