본 시험은 이탈리안 라이그라스 극조생종 품종을 육성하기 위하여 2001년부터 2011년까지 국립축산과학원 초지사료과에서 수행되었다. 이탈리안 라이그라스 신품종 그린팜2호는 2배체 작물로 엽은 녹색이며, 월동 전 초형은 반직립형, 봄의 초형은 직립형이다. 그린팜 2호는 4월 28일경에출수하는 극조생종 품종이다. 또한, 그린팜 2호는 플로리다80 보다 지엽폭이 0.6 mm 넓고, 지엽의 길이가 1 cm 짧으며, 출수기의 초장은 94 cm로 플로리다 80 보다 1 cm 정도작다. 줄기 두께는 플로리다 80 보다 0.7 mm로 굵고 이삭길이는 플로리다 80 보다 2.2 cm 짧았다. 그린팜 2호의 건물수량은 11,452 kg/ha로서 플로리다 80과 대등하였다. 그린팜 2호의 상대적 사료가치는 플로리다 80이 97인데 비해그린팜 2호는 114로 월등히 높고 in vitro 소화율이 70.6%,가소화양분총량(TDN)이 63.9%로서 플로리다 80 보다 각각 1.6, 2.8% 높았고, 산성세제불용섬유(ADF)와 중성세제불용섬유(NDF)는 31.7 및 52.6%로서 플로리다 80 보다 각각 3.5% 및 6.4% 낮았다.

Background : Five medicinal herbs have been selected from the preliminary screening for in vitro anti-allergy activity (in RBL-2H3 cells). The present study is conducted to investigate the inhibitory effects of the medicinal herbs on allergic inflammation in other kind of cells. Methods and Results : Cells treated with five extracts prepared from Betula costata Trautv. (aerial part), Camellia sinensis L. (aerial part), Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) were measured for mRNA levels of TNF-α on HaCaT keratinocytes stimulated by TNF-α /INF-γ and for mRNA levels of IL-2 in Jurkat T cells mediated by PMA/A23187. Pre-treatment with the five extracts reduced the mRNA levels of TNF-α in HaCaT cells and mRNA levels of IL-2 in Jurkat T cells. In particular, the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai significantly and dose-dependently decreased the mRNA levels of TNF-α and IL-2. To determine the toxicity of the extracts from the selected medicinal herbs to HaCaT cells and Jurkat T cells, the viabilities of the cells treated with several concentrations of the five extracts were measured by MTT assay. Extracts of Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) (up to 250 ㎍/㎖) did not show cytotoxic effects on HaCaT cells and Jurkat T cells. On the other hand, 250 ㎍/㎖ of extracts of Betula costata Trautv. (aerial part) and Camellia sinensis L. (aerial part) reduced cell viability in both cells. Conclusion : These results demonstrate that the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai has an anti-inflammatory effect by inhibiting pro-inflammatory cytokine expression. Therefore, the leaf of Pyrus pyrifolia var. culta (Makino) Nakai can be a useful resource for the development of anti-allergy/anti-inflammatory materials.