Climate change may result in increases in air temperature and shifts in precipitation patterns as the result of increases in atmospheric CO2. Outdoor, naturally sunlit, plant growth chambers referred to as SPAR (Soil Plant Atmosphere Research) chambers are one of the important research facilities under controlled conditions. SPAR provide precise control of the major environmental variables influencing crop growth including temperature, humidity and atmospheric CO2 concentration. Especially the SPAR system is considered as the most realistic approach for studying crop canopy gas exchange including photosynthesis, respiration, transpiration. Indeed, many reports have confirmed that gas exchange values in SPAR is highly associated with dry matter and yield. So SPAR data can be used to develop quantitative relationships and functions, which are then tested against field data. In ARS, the SPAR system is widely used for research on: (1)Response and adaptation of crops and weeds to elevated CO2 and global warming, (2)Developing analytical and management strategies to improve crop utilization and to reduce losses due to the environment, (3)Mechanistic process-level crop simulation models for assessment of agricultural systems.

This study was conducted to clarify the effect of high temperature during winter period(autumn sowing) and spring sowing on yield, quality and growth and development in barley. The varieties used for the experiments were heenchalssalbori and keunalbori 1 having a strong spring habit characteristics. In spring sowing treatment, spikelet differentiation was proceeded rapidly and tillering was proceeded slowly compared to the development stage, because the barley sowed at spring is cultivated in high temperature and long day conditions from sowing to spikelet differentiation stage compared with autumn sowing(control). And in high temperature treatment during winter period, like spring sowing, tillering was inhibited compared to the development stage. The number of grain per panicle and the period required to heading stage from spikelet differentiation were reduced largely at spring sowing, because spring sowing treatment was conducted in high temperature and long day condition compared with autumn sowing and high temperature treatment during the period from spikelet differentiation to heading stage. Meanwhile in spring sowing treatment, average temperature during ripening stage was higher than the autumn sowing and high temperature during winter, because heading stage was so late. After all, starch, amylose content and grain weight were reduced while protein content was relatively increased in spring sowing treatment due to difference of average temperature of ripening stage. These changes affected the decrease of viscosity of peak, trough, breakdown and the increase of setback viscosity

This experiment was conducted to clarify the effect of the high temperature on physicochemical properties of barley kernels during ripening stage. High temperature treatment was lasted from each 10, 17 and 24 days after heading(DAH) until the harvest time at 21oC, 24oC, 27oC in artificial climate room. The results showed that ripening period from heading to maturity was tend to be shorter at higher temperature treatment condition and at longer duration treatment condition. and 1000-grain weight was decreased as the ripening period shortened. Furthermore, gelatinization properties was changed by high temperature due to the reduction of starch and amylose contents. As the shortening of grain filling period by a high temperature treatment, the protein content was increased. In the 10 DAH at 27oC treatment, the grain filling period was shortened by 9 days. The starch contents was reduced by 5.7 %, and the protein content was increased by 5.6 % in a such condition. Protein contents was showed negative correlations with amylose, starch contents and gelatinization properties, respectively. Starch contents, however, showed positive correlations with amlyose content and gelatinization properties.

키다리병 내성벼의 생리·생화학적 특성 연구를 알아보기 위해 남평벼와 호품벼를 비교하여 실시한 실험결과는 다음과 같다. 1. 키다리병 발병률은 품종에 관계없이 병원균 포자의 농도,노출시간에 비례하여 증가하며 특히 감수성 품종인 호품벼는그 증가 속도가 더욱 심함을 알 수 있었고 이들 감수성 품종들은 염수선을 통한 종자정선이 키다리병 예방에 중요한 역할을 한다. 2. 키다리병 감염조건에서 남평벼가 호품벼에 비해 체내 전분 및 단백질 감소 정도가 더 작았으며 α-amylase, CAT,SOD 항산화 효소 활성은 다소 높게 유지되었다. 3. 키다리병 감염조건에서 SOD를 제외한 CAT, POD, APX유전자들은 남평벼가 호품벼에 비해 모두 2 ~ 3배 이상의 강한 발현증가를 보인 반면 호품벼에서 GA positive gene인Gα(D1), GID2의 발현이 높은 반면 GA deactivating gene인EUI의 발현은 남평보다 낮은 것으로 나타났다.

신간척지에서 총체사료용 벼 주요 품종들의 담수직파 적응성을 평가하기 위하여 2012~2013년에 시험한 결과를 요약하면 다음과 같다.1. 입모율은 녹양과 수원552호 48%, 목우, 목양 및 수원544호 40~42%이었다. 2. 출수기는 녹양과 목양 8월 26~27일, 수원552호와 수원544호 9월 1~5일, 목우 9월 15일이었다. 3. m2당 수수는 278~333개였으며, 도복은 수원552호에서경미하게 발생하고, 그 외 공시품종에서는 발생하지 않았다.4. ha당 건물수량은 목우와 수원552호 15.8~16.8톤, 수원544호, 목양 및 녹양 이 13.1~14.3톤이었다.5. 조단백질 함량은 4.5~6.4%, ADF 함량은 27.1~30.1%,NDF 함량은 49.2~53.9%, TDN 함량은 65.1~67.4%이었다.6. 따라서 신간척지에서 총체사료용 벼의 담수직파재배에 가장 적합한 품종은 목우와 수원552호 이었다.

본 연구는 간척지에서 밀과 헤어리베치 혼파에 따른 녹비 생산성 향상 구명을 위하여 수행하였다. 새만금간척지에서 동계 녹비작물 헤어리베치와 밀 혼파 재배시 관행구(헤어리베치 30 kg ha-1)와 혼파구는 관행구 대비 헤어리베치 40%감축(헤어리베치 20+ 밀 50 kg ha-1), 40%증량(헤어리베치 40+ 밀 50 kg ha-1), 100%증량(헤어리베치 60+ 밀 50 kg ha-1) 하여 각각 파종하고 토양화학성, 생육 및 생산성을 검토한 결과는 다음과 같다. 공시토양은 유기물, 유효인산 함량이 적었으며 치환성마그네슘 및 나트륨 함량이 많은 약산성 염류토양 이었다. 생육은 혼파 재배에서 양호하였고, 초장은 관행 (101cm) 대비 30∼44% 길었으며 경직경은 관행 (2.75mm) 대비 19∼26% 두꺼웠다. 식물체 중 무기양분 함량은 혼파 재배시 질소, 인산, 가리 함량이 증가하고 탄소 함량이 감소하는 경향을 나타냈다. 질소생산성은 단파구 (90kg ha-1)에 비하여 혼파구에서 94∼107 kg ha-1 로 많은 반면에 탄질률은 단파구 (14.6)에 비하여 혼파구에서 12.8∼14.2로 낮았다. 생초수량은 헤어리베치 단파구 (18,790 kg ha-1)에 비하여 헤어리베치와 밀 혼파구에서 18,740∼22,080 kg ha-1로, 건물수량은 헤어리베치 단파구 (3,120 kg ha-1)에 비하여 헤어리베치와 밀 혼파구에서 3,110∼3,650 kg ha-1로 나타났다. 따라서 새만금 간척지에서 두과 녹비작물 헤어리베치와 밀의 혼파로 높은 질소 생산성 및 낮은 탄질률로 녹비효과 증진과 혼파재배로 농업생태계 다양화 및 유휴 농경지 녹색경관 조성을 기대할 수 있었다.

새로 조성된 간척농지의 토양개량을 위하여 새만금간척지에서 2007년부터 2011년까지 4년 동안 녹비작물 (여름철 : 세스바니아, 제주재래피, 수수×수단그라스, 겨울철 : 귀리, 호밀, 보리) 재배 및 시용이 토양이화학성에 미치는 영향을 조사하여얻은 결과는 다음과 같다.1. 녹비작물의 바이오매스 생산량은 평균 9.7~15.0ton/ha이었으며, 이 중에서 수수×수단그라스의 생산량이 가장 많았다.2. 녹비작물의 재배와 시용으로 토양유기물 함량이 1.3에서6.0g/kg로 매년 평균 1.2g/kg씩 증가하였다.3. 녹비작물의 재배와 토양환원에 의해 토양의 용적밀도가1.44에서 1.24Mg/m3로 감소하였다.4. 수수×수단그라스와 호밀의 이어짓기가 토양 용적밀도 감소에 가장 효과적이었고, 세스바니아 재배는 토양 질소함량 증가에 가장 효율적이었다.이상의 결과로 미루어 볼 때 간척지에서의 녹비작물 재배및 시용은 토양의 비옥도 요인을 일부 개선시킴으로써 간척농지 개량에 기여할 수 있을 것으로 기대된다. 앞으로 신간척지에서의 유기물과 관련된 다각적인 연구가 필요할 것으로 생각된다.

벼 흰잎마름병균의 정확한 진단을 위하여 PCR용 진단 kit를 개발하였다. 본 PCR kit를 개발하기 위하여 벼 흰잎마름 병균 유전체 정보 중 phage-related integrase and transposase gene의 염기서열을 이용하여 프라이머를 각각 제작하였다. 프라이머 염기서열은 XOP-F (5-CGG TCT GCT CAA TGA GGA AGA-3)와 XOP-R2 (5-TGC AAT TGG TGT TCTCCA GG-3), XOT-F (5-GTC ATA GGT GAG GCT TCCC-3)와 XOT-R2 (5-AGT GCG ATC TTT CAG CAG G-3)로 벼 흰잎마름병균의 DNA를 401bp와 492bp를 증폭하게 제작하였다. PCR 증폭은 벼 흰잎마름병균만 증폭하였으며 다른 세균인 Escherichia coli, Agrobacterim, Pectobacterium caratovora subsp. cartovorum, P. atrosepticum, Pseudomonasputida, P. syringae, P. savastanoi pv. phaeolicola, P. savastanoipv. savastanoi and P. marginalis pv. Marginalis 등은증폭되지 않아 특이성이 인정 되었다. 본 프라이머로 병이 의심되는 벼잎과 논물에서 병원균을 3시간 이내에 검출할 수 있었다.

This study was carried out to know whether the host plant feeding have an influence to subsequent prey consumption of a zoophytophagous mirid, Nesidiocoris tenuis, with biological control potential. Piece of leave of Paprika, Sesame, and cotton ball soaked with water were respectively provided to the test insects, adult mirid starved about a day, and frozen eggs of Ephestia kuehniella, were presented to them for a day and counted the number of the eggs consumed by them. The mirid fed by Sesame leave took significantly less prey than both the Paprika and the water fed one.

Maize weevil (Sittophilus zeamais) and Indian meal moth (Plodia interpunctella) are the dominant species among the stored grain insect pests of the rice grain and bran. This experiment was conducted to investigate developmental characteristic and damages of S. zeamais and P. interpunctella on the rice. Under five constant temperatures, 15, 20, 25, 30 and 35℃, developmental periods from egg to adult of Sittophilus zeamais were 43.0, 36.4, 29.2, 20.8 and 16.3 days, respectively. With egg periods being 9.6, 7.3, 5.2, 3.2 and 2.6 days, and larval periods being 25.2, 22.6, 19.8, 14.5 and 11.3 days, and adult periods being 129.3, 116.1, 108.6, 89.2 and 73.3 days, respectively. Damages of S. zeamais adult at 15, 20, 25 and 30℃ were 67.2, 96.2, 134.0 and 174.5 for 24hr on the rice. Damages of P. interpunctella larval 15, 20, 25 and 30℃ were 56.2, 78.3, 109.4 and 138.7 for 24hr on the rice. The duration of maximum occurrence were June to August for S. zeamais, late May to early August for P. interpunctella.

Insecticidal activity of active component isolated from Ruta chalepensis leaves was examined against maize weevil, Sitophilus zeamais and compared with two different bioassay system, such as direct contact and fumigant method. The methanol extract of R. chalepensis leaves had strongly (+++) insecticidal activity at 50 mg/disk against S. zeamais. Methanol extract of R. chalepensis was partitioned with hexane, chloroform, ethyl acetate, butanol and water fraction, successively. In this result, the highest activity was shown in chloroform fraction against S. zeamais. Biologically active compound derived from chloroform fraction of R. chalepensis extract was purified by using SiO2 column chromatography and prep-HPLC. The insecticidal constituent of R. chalepensis was identified as quinoline-4-carboxaldehyde by various chromatography and spectroscopic analysis methods. At 2.5 mg/disk, the most toxic activity against S. zeamais was exerted by the direct contact method (100%), followed by the fumigant method (23%). These results revealed that the contact toxicity showed 4.35 times greater than the respiration toxicity. Furthermore, these results indicate that quinoline- 4-carboxaldehyde could be useful as a new preventive agent against damage caused by stored-product insects.

The insecticidal effect for several commercially available environment friendly agricultural materials (EFAMs) is investigated to Cnaphalocrocis medinalis, Narana aenescens, Pseudaletia unipuncta and Parnara guttata on rice. In the insecticidal effects for 2-3rd instar larvae of lepidopteran after treatment with 19 EFAMs, more than 95% of mortality rate showed 5 EFAMs in C. medinalis, 12 EFAMs in N. aenescens, 9 EFAMs in P. unipuncta, and 14 EFAMs in P. guttata, respectively. Among 4 EFAMs selected for C. medinalis in indoor, more than 70% in control effect is seen EFAM-B(85.2%), EFAM-I(79.1%) and EFAM-M (78.3%), respectively, in rice field. The insecticidal effect for EFAMs is investigated to Nilaparvata lugens, Laodelphax striatellus and Nephotettix cincticeps on rice. In the insecticidal effects for 2-3rd nymph and adult after treatment with 19 EFAMs, more than 95% of mortality rate showed 5 EFAMs in N. lugens adult, 7 EFAMs in N. lugens nymph, 6 EFAMs in L. striatellus, and 5 EFAMs in N. cincticeps, respectively. Insecticidal effect for N. lugens was sustained until 10 day in rice treated with 7 EFAMs selected in indoor. Among them, EFAM-L, EFAM-P and EFAM-R was sustained in low density until 27 day. But EFAM-D, EFAM-L, EFAM-N and EFAM-S showed high density until 27 day and additional control was needed.

Biological control of rice insect pest is an important component of an IPM program. There are many species of natural enemies which contribute to the suppression of rice pest populations below economic injury levels. In order to use biological control more efficiently, it is a need to identify beneficial species and determine their roles in possible regulation of insect pests. There is a rich complex of biological control agents in rice and bund. This research was carried out to investigate the population density of insect pests and natural enemies in the rice field and bund. A total of 7 pest species and 15 natural enemy species were collected in the rice field. 10 pest species and 20 natural enemy species were collected in the bund, also. Changes in population density of insect pests and its prey were investigated in the rice field and bund. Population densities of insect pests were low at any time during the rice growth period in the field. This could be the high density of natural enemies. Bunds served as refuge for natural enemies when rice maturity. There are rich complex of biological control agents in rice field and bund. So, when we practice integrated pest management(IPM) of rice insect pests, we should use various natural enemies.

We have gathered rice grain and bran from conventional mills and RPC(Rice Processing Complex) for the survey of stored grain insect pests. Maize weevil(Sittophilus zeamais) is the dominant species among the stored grain insect pests of the rice grain and bran. Under three constant temperatures, 15, 20 and 25℃, developmental periods from egg to adult were 43.0, 37.5 and 29.2 days, respectively. With egg periods being 9.6, 7.3 and 5.2days, and larval periods being 25.2, 21.8 and 19.8days, and adult periods being 126.1, 110.3 and 108.6days, respectively. The adults of Maize weevil feed basically on the same the foods as the larvae but not as restricted in their diets because the larvae need to develop inside whole grains. Feeding of S. zeamais adult usually began 3∼4 days after emergence at 25℃. Then adult feeding ca. one rice a day, reaching a peak of 20∼40days after emergence.

The occurrence and damage of stink bugs were monitored at South Korea from 2007 to 2008. A total of 11 stink bug species were observed in paddy field and occurrence periods of stink bugs were significantly differed by species. In occurrence period and damage of stink bug feeding in paddy field, Scotinophara lurida occurred from June, Cletus punctiger, Eysarcoris aeneus, Rhopalus maculatus, Riptortus clavatus, E. ventralis, Stenotus rubrovittatus occurred from August and Pachygrontha antennata, C. schmidti, Trigonotylus caelestialium, Paromius exiguus occurred from September. When we were classified into two region with mountain and plain area cultivated rice, generally C. puntiger population was higher in mountain area than plain area, and E. aeneus was higher in plain area than mountain area. Also, stink bug species were higher in environment condition formed with a paddy field and a waterway overgrown with weeds. Control threshold for E. aeneus in paddy field was 1-2 individuals/heading stage, and then rate of pecky rice made to 5.0% while milk stage, dough stage and yellow ripe stage made to less than 5.0%.

The green rice leafhopper, Nephotettix cincticeps is an insect vector which transmits virus diseases of rice plant causing severe damage to the rice and its damage both quality and yield of rice. Survival rate of overwintering N. cincticeps in Alopeculus acquadis among 15 species of the winter crops and weeds was 37.8% and its most preferred. Density of N. cincticeps in environment-friendly agriculture area which cultivated Astragalus sinicus as a green manure crop in winter season increased in number wintering pass in Alopeculus acquadis and Astragalus sinicus as host plants, because of control of N. cincticeps was not enough in the previous year. Survival rate of N. cincticeps was higher mainly in habitation mixed with rice straw, Astragalus sinicus and Alopeculus acquadis as winter habitation. It warmed due to the effect of the keeping warmth inside of a rice paddy field and the bank around a rice field covered with rice straw or Astragalus sinicus. Also, habitation conditions were optimal for survival because of growing Alopeculus acquadis as the major host plant of N. cincticeps in that place.

실험실 조건에서 고구마 뒷날개흰밤나방의 교미 전 행동습성을 관찰하였다. 짝이 있고 어두운 상태에서 안테나를 치켜세우고 날개를 진동하는 습성을 보였으며, 암컷과 수컷 모두 날개를 진동하고 안테나를 치켜세우는 행동을 보이다가 수컷이 암컷에게로 날아가는 습성을 보였다. 불이 꺼진상태로 20여분이 지나면 안테나를 치켜세우는 반응을 보이기 시작하였으며 40분이 지났을 때부터 마치 짝을 유인하는 음향을 생성하듯이 날개를 빠르게 진동하는 반응을 보였다. 짝짓기는 우화 후 이튿날부터 관찰되었으며 대부분 4일에서 6일 사이에 짝짓기를 하였다. 짝짓기는 평균 207분 동안 지속되었고, 대부분 3회까지 반복적으로 교미를 하였다. 관찰결과를 종합적으로 분석해보면 암컷이 신호를 보내고 수컷이 이에 반응하는 것으로 보이며, 암컷이 내는 음향이 뒷날개흰밤나방의 교미전 행동습성에 관련되어 있을 가능성이 높은 것으로 판단되었다.

익산지역에서 벼 줄점팔랑나비(Parnara guttata)는 1년에 3세대를 경과하며, 5월중 하순부터 월동처에서 우화하여 논으로 이동한 다음 벼 잎에 산란하기 시작하였다. 유충에 의한 벼의 피해는 7월 하순부터 증가하여 8월 중 하순 사이에 가장 많은 것으로 관찰되었으며, 3세대 성충이 우화하기 시작하는 시기는 9월 중순이었다. 벼의 이망시기와 줄점팔랑나비에 의한 피해는 만식한 논에서 켰다. 살충제를 유충발생 최성기 약 1주일 전인 7월 하순에 처리하였을 때 91.2%의 방제효과를 나타낸 반면 8월 초순에 처리할 경우에는 방제효과가 57.2%로 낮았다.

Limonium tetragonum is a halophyte grown naturally in the coastal region in South Korea. This study was conducted to investigate the effects of salt concentrations on seed germination, seedling growth, and antioxidant capacity of L. tetragonum. Seeds were collected from naturally grown plants of L. tetragonum and those at full maturity were used in this experiment. All experiments were performed at 0%, 0.5%, 1.0%, or 2.0% of salt concentrations. Seed germination rate was highest as 86% at 20℃ and followed as higher in order of 25℃, 30℃ and 15℃. The germination rate was about 80% at 0% or 0.5% of salt concentration, but it was very low at the salt concentrations higher than 1%. Growth of L. tetragonum seedlings showed no difference in Hoagland solution containing NaCl in the range of 0% to 1.0% and seedlings survived at 2.0% of NaCl concentration. As the salt concentration increased, the content of Na+ in the shoot increased, but that of K+, Ca++, or Mg++ decreased. The antioxidant activity and the content of total polyphenol and total flavonoid in the shoot were similar at 0% and 0.5% of NaCl and were highest at 2.0% of NaCl concentration. In conclusion, performance of seed germination and plant growth of L. tetragonum was highest at 0% and 0.5% of NaCl concentration, and showed no difference in antioxidant activity, total polyphenol contents, and total flavonoid contents at the same salt concentrations.

This experiment was conducted to evaluate the effects of high temperature on the stem, leaf and grain of barley during the ripening period and to provide information for the development of high-temperature cultivation techniques and adaptive varieties. We used an artificial climate control facility, to provide a temperature 3℃ higher than the normal average temperature during the ripening stage. Although the maximum rate of starch synthesis was increased at high temperature by approximately 11%, the starch content was decreased, because the period of starch synthesis ended 4 days earlier. As in the case of starch synthesis, the expression of genes related to starch synthesis was increased at the early ripening stage in the high temperature treatment, however, the duration of expression tended to decrease rapidly. Furthermore, the partitioning rate of assimilation products in the panicle increased to a greater extent in the high temperature treatment than in the control. In contrast, for the stem and leaf, the partitioning rate of assimilation products decreased more rapidly in the high temperature treatment than in the control. On the basis of these results, it can be considered that the translocation rate of assimilation products increased to a greater extent in the high temperature treatment than in the control at the early ripening stage. These results indicate that the decrease in grain weight at high temperature during the ripening stage is attributable to an increase in the speed of starch synthesis at high temperature, but the increase in ripening speed does not compensate for the shortening of the ripening period. Finally to develop varieties and cultivation techniques suited to high temperature, we need to focus on physiological characteristics related to the duration of starch synthesis.