Injection molding is a process of shaping resin materials by heating them to a temperature above their melting point and then using a mold. The resin material is injected into and cooled within the mold cavity, solidifying into the desired shape. The core and cavity components that make up the mold cavity are crucial elements for the precision molding in injection molding. In the case of precision mold production, the application of 5-axis machining technology is required to ensure high machining quality for complex shapes, and among these factors, the tool angle is a critical machining condition that determines the surface roughness of the workpiece. In this study, we aim to measure the surface roughness of the machined surface of KP4A specimens during machining processes with variations in the tool angle and analyze the correlation between the tool angle and surface roughness.

The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ≥ 0.3 O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.

본 연구는 특용작물인 인삼을 대상으로 식물공장에서의 LED 광원, Hertz와 Duty비에 따른 생리·생태학적 반응을 알아보고자 하였다. 본 실험에서는 LED 시스템을 이용하 여 광원의 종류를 단색의 적색광, 청생광, 백색광, 황색광, 원적색광 그리고 적색+청색 혼합광, 적색+청색+백색 혼합 광, 적색+원적색 혼합광으로 총 16개의 구배로 구성하였다. 적색+청색 혼합광은 Hertz를 각각 20, 60, 180, 540, 1620, 4860Hz로 처리하였고 적색+청색+백색 혼합광은 Hertz를 각각 60, 180, 540, 1620Hz로 처리하여, 이 실험을 각각 Duty비 30%, 50%, 70%로 총 3번의 실험을 진행하였다. 실험 결과, 인삼의 광합성률은 Duty비 30%일 때 적색+ 청색 혼합광의 20Hz에서 가장 높았고, Duty비 50%일 때 적색+청색 혼합광의 60Hz에서 가장 높았으며, Duty비 70%일 때 적색+청색 혼합광과 적색+청색+백색 혼합광의 60Hz에서 높았다. 인삼의 증산률은 Duty비 30%일 때 적색 +청색 혼합광의 20Hz에서 가장 높았고, Duty비 50%일 때 백색광의 180Hz에서 가장 높았으며, Duty비 70%일 때 청 색광과 백생광의 180Hz가 높았고 적색+청색 혼합광의 20Hz와 540Hz, 적색+청색+청색 혼합광의 60Hz가 높았다. 인삼의 수분이용효율은 Duty비 30%일 때 적색+청색 혼합 광의 180Hz에서 가장 높았고, Duty비 50%일 때 적색+청색 혼합광의 60Hz에서 가장 높았으며, Duty비 70%일 때 황색 광의 180Hz가 높았고 적색+청색 혼합광과 적색+청색+백 색 혼합광의 1620Hz에서 가장 높았다. 위의 결과를 종합적으로 보았을 때 인삼을 식물공장의 LED광 조건으로 재배할 경우 Duty비 50%에서 가장 경제 적인 생산에 최적으로 판단된다.

In this study, we established the pre-treatment conditions that could increase the γ-aminobutyric acid (GABA) content during barley germination. In the process, three different barley samples were prepared, which differed in the pre-treatedment processes. The specimens were stored at 50 for 1 h after being kept in water at room temperature for 4 h (HKW sample), kept in sufficient water for 4 h (KW sample), or left untreated (CO sample). After the pre-treatment, the barely samples were germinated for 35 h. A sample was taken from each batch in 5-h intervals, extracted with water, and physicochemical characteristics and radical scavenging activity were measured. As a result, we found that the contents of phenolic compounds (18.02-30.63 mg/100 g) and flavonoids (1.87-4.63 mg/100 g) were higher in HKW, showing similar trends. Also the GABA contents in the HKW and KW samples were higher than that in the CO sample. Furthermore, the radical scavenging activities of DPPH and ABTS were the highest in the HKW sample, having values in the ranges of 58.49-77.40% and 54.57-88.10%, respectively. All in all, we found that in order to increase the antioxidative activity and GABA content of the barley samples, it would be suitable to pre-treatment the specimens after the post-immersion heat treatment. In addition, pre-treating the KW samples is appropriate only after immersion time. Lastly, the optimum germination time of the batches was found to be 20-25 h.

This study investigated the antioxidant and antidiabetic activities of Stachys sieboldii Miq. extracts by solvents (water, ethanol, butanol, chloroform, and hexane). The contents of total polyphenols (7.18-37.25 mg/g) and flavonoids (0.21-5.21 mg/g) in extracts from Stachys sieboldii Miq. showed a significant difference dependent on the extraction solvents, butanol > ethanol > water > chloroform > hexane. Antioxidant activities by DPPH and ABTS radical scavenging were increased in a dose-dependent manner. These activity trends associated with the extraction solvent were different at each concentration, but resembled phenolic compound contents trend, generally. FRAP value increased in a dose-dependent manner, but there was a difference in radical scavenging activities when comparing between extraction solvents by butanol > ethanol > hexane > chloroform > water on all concentrations. The trend of α-amylase inhibition of extracts from 1,000 μg/mL to 2,000 μg/mL was not affected as enzyme activity is promoted and not inhibited. The inhibition of α-glucosidase was increased in a dose-dependent manner without water extracts, the activity on hexane extracts was higher than others per the extraction solvent. α-Glucosidase inhibition of hexane extracts showed 57.76% at 250 μg/mL, which is 2.8 times higher than the second highest chloroform extract (20.65%). From these results, we presume that the active ingredients of Stachys sieboldii Miq. is different according to the extraction solvent and also the activity is different by these major functional groups.